Prupe.4G283900_v2.0.a1

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0001358
Physical Location & Seq
Forward (+)
24528689 .. 24532571
3883 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G283900.1

Sequence Viewer

Length: 1482 bp
ATGGCTGCTAGTCCTCCTCCATATAACCCCGTGCCGGTTGCGTACCAGGGAGGTGCCGGCGCGGTGCCAGTCTGGTTGAACAAAGGGGACAACGCATGGCAAATGGTAGCAGCCACACTCGTCGGCCTCCAAAGCGTGCCGGGTCTTGTCATTCTGTACGGAAGCATAGTAAAAAAGAAATGGGCAGTGAACTCAGCTTTCATGGCTCTCTACGCTTTTGCGGCGGTCGTAATCTGCTGGGTGTCGTGGGCTTACAAGATGTCGTTTGGGGACAAGCTCCTACCCTTCTGGGGCAAAGCTGGCCCTGCCTTGGGCCAGAAGTTCCTCATCAACCAGGCAGCTCTGCCAGCGAGCACGCACTACTTCAGCGACCACAAGACTGTGGAGACTGCTGAGATCACACCCTGGTACCCGATGGCGACCATGGTGTGGTTTCAGTGCGTGTTTGCAGCCATTACGCTGATACTGCTGGCGGGGTCGGTGCTTGGGAGGATGAACTTCAAGGCGTGGATGATGTTCGTGCCGCTTTGGCTTACATTTTCGTACACTGTCGGAGCGTTCAGCTTGTGGGGTGGAGGCTTCTTGTTCCATTGGGGTGTCATGGACTATTCCGGTGGTTATGTCATTCATCTTTCTTCCGGGATTGCTGGCTTCACCACTGCTTACTGGGTAGGACCAAGGGTGAAGAAGGACAGGGAGAGATTTCCACCAAACAACGTACTTCTGGTGTTAGCCGGAGCCGGGTTGCTGTGGATGGGATGGGCAGGTTTCAACGGGGGAGATCCGTACGCGGCCAACACGGACTCATCCATGGCCGTCCTCAACACCAACATCTGCGCCGCCACGAGCTTCTTGGTTTGGACTTGGCTCGATGTCATCTTCTTTGAGAAGCCTTCCGTCATTGGAGCTGTCCAAGGAATGATCACTGGCCTCGTATGCATTACTCCTGCTGCAGGTCTTGTTCAAGGATGGGCCGCTATAGTGATGGGAGTTATGTCAGGCAGTGTGCCATGGTTCACCATGATGATAGTGGACAAGAGGTGGAAACTGCTATCCGCAGTTGACGACACCCTTGGTGTAGTTCACACCCACGCCGTGGCCGGGTTTCTTGGCGGCGTTTTAACGGGGCTTTTCGCTGAGCCTGAACTATGTGCACTCTTTTTGCCCGTCACAAACTCGCGCGGAGGCGTGTACGGAGGCTCAGGAGGAATGCAAATCCTCAAACAAATTGTGGGAGCCCTATTTATCATTGGATGGAATCTTGTGGTCACCTCCATTATTTGCCTAGTCTTACGGTGTATAGTTCCGTTGCGCATGCCGGAGGAACAGTTGCTGATTGGGGATGACGCAGTGCATGGAGAAGAGGCATATGCATTGTGGGGTGATGGGGAGAAGTATGATGTTACTAGGCATGAGCTTTACTCCGATGATACGTCACACGCTCAAAAATATCCATCCAGTGGTGCCACTCAGGTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

494

Amino Acids

53.26

Weight (kDa)

6.5

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1313
Acc36I ACCTGC 2 cut(s) 755, 944
Acc65I GGTACC 1 cut(s) 408
AccB1I GGYRCC 4 cut(s) 53, 64, 408, 1463
AccB7I CCANNNNNTGG 3 cut(s) 429, 1096, 1460
AccII CGCG 4 cut(s) 62, 791, 1180, 1182
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 3 cut(s) 792, 813, 1098
AcuI CTGAAG 1 cut(s) 349
AdeI CACNNNGTG 1 cut(s) 1096
AfaI GTAC 7 cut(s) 44, 158, 410, 545, 720, 788, 1193
AgsI TTSAA 4 cut(s) 79, 502, 772, 965
AjnI CCWGG 3 cut(s) 45, 333, 404
AleI CACNNNNGTG 1 cut(s) 1472
AluBI AGCT 8 cut(s) 197, 277, 299, 341, 564, 849, 908, 1417
AluI AGCT 8 cut(s) 197, 277, 299, 341, 564, 849, 908, 1417
Alw21I GWGCWC 2 cut(s) 356, 1156
Alw26I GTCTC 1 cut(s) 380
Alw44I GTGCAC 1 cut(s) 1152
AlwI GGATC 1 cut(s) 776
AlwNI CAGNNNCTG 1 cut(s) 1333
AoxI GGCC 8 cut(s) 124, 301, 313, 792, 813, 928, 972, 1098
ApaLI GTGCAC 1 cut(s) 1152
ApeKI GCWGC 5 cut(s) 5, 110, 338, 449, 950
Asp718I GGTACC 1 cut(s) 408
AspLEI GCGC 4 cut(s) 62, 839, 1182, 1314
AspS9I GGNCC 4 cut(s) 302, 313, 674, 972
AsuC2I CCSGG 4 cut(s) 141, 640, 742, 1102
AsuHPI GGTGA 5 cut(s) 646, 694, 1009, 1261, 1394
AvaII GGWCC 1 cut(s) 674
BaeGI GKGCMC 1 cut(s) 1156
BaeI ACNNNNGTAYC 2 cut(s) 392, 425
BanI GGYRCC 4 cut(s) 53, 64, 408, 1463
BanII GRGCYC 1 cut(s) 1240
BauI CACGAG 1 cut(s) 844
Bbv12I GWGCWC 2 cut(s) 356, 1156
BbvI GCAGC 4 cut(s) 122, 350, 461, 937
BccI CCATC 8 cut(s) 409, 748, 753, 963, 979, 1248, 1379, 1462
BceAI ACGGC 2 cut(s) 800, 1079
BciT130I CCWGG 3 cut(s) 47, 335, 406
BclI TGATCA 1 cut(s) 921
BcnI CCSGG 4 cut(s) 141, 640, 742, 1102
BcoDI GTCTC 1 cut(s) 380
BfaI CTAG 3 cut(s) 9, 1286, 1407
BfmI CTRYAG 2 cut(s) 951, 978
BfuAI ACCTGC 2 cut(s) 755, 944
BglI GCCNNNNNGGC 1 cut(s) 529
BlpI GCTNAGC 1 cut(s) 1137
Bme1390I CCNGG 7 cut(s) 47, 141, 335, 406, 640, 742, 1102
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 4 cut(s) 302, 313, 674, 972
BmiI GGNNCC 6 cut(s) 55, 66, 410, 739, 1237, 1465
BmrFI CCNGG 7 cut(s) 47, 141, 335, 406, 640, 742, 1102
BmrI ACTGGG 1 cut(s) 676
BmuI ACTGGG 1 cut(s) 676
BplI GAGNNNNNCTC 2 cut(s) 1406, 1438
Bpu10I CCTNAGC 1 cut(s) 1201
Bpu1102I GCTNAGC 1 cut(s) 1137
BpuMI CCSGG 4 cut(s) 141, 640, 742, 1102
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 2 cut(s) 1313, 1343
Bse118I RCCGGY 2 cut(s) 34, 56
Bse1I ACTGG 4 cut(s) 68, 671, 931, 1458
BseBI CCWGG 3 cut(s) 47, 335, 406
BseGI GGATG 9 cut(s) 498, 516, 759, 764, 806, 974, 1259, 1348, 1454
BseMII CTCAG 4 cut(s) 207, 384, 1128, 1215
BseNI ACTGG 4 cut(s) 68, 671, 931, 1458
BseRI GAGGAG 1 cut(s) 6
BseSI GKGCMC 1 cut(s) 1156
BseXI GCAGC 4 cut(s) 122, 350, 461, 937
BseYI CCCAGC 1 cut(s) 237
Bsh1236I CGCG 4 cut(s) 62, 791, 1180, 1182
Bsh1285I CGRYCG 1 cut(s) 228
BshFI GGCC 8 cut(s) 126, 303, 315, 794, 815, 930, 974, 1100
BshNI GGYRCC 4 cut(s) 53, 64, 408, 1463
BsiEI CGRYCG 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 356, 1156
BsiSI CCGG 9 cut(s) 35, 57, 140, 612, 639, 735, 741, 1101, 1319
BsiWI CGTACG 1 cut(s) 786
BslFI GGGAC 2 cut(s) 101, 284
BsmAI GTCTC 1 cut(s) 380
BsmFI GGGAC 2 cut(s) 101, 284
BsmI GAATGC 1 cut(s) 1215
BsnI GGCC 8 cut(s) 126, 303, 315, 794, 815, 930, 974, 1100
Bsp1286I GDGCHC 3 cut(s) 356, 1156, 1240
Bsp143I GATC 3 cut(s) 396, 781, 921
Bsp1720I GCTNAGC 1 cut(s) 1137
Bsp19I CCATGG 3 cut(s) 423, 810, 1010
BspANI GGCC 8 cut(s) 126, 303, 315, 794, 815, 930, 974, 1100
BspCNI CTCAG 4 cut(s) 206, 385, 1129, 1214
BspFNI CGCG 4 cut(s) 62, 791, 1180, 1182
BspLI GGNNCC 6 cut(s) 55, 66, 410, 739, 1237, 1465
BspMAI CTGCAG 1 cut(s) 955
BspMI ACCTGC 2 cut(s) 755, 944
BspPI GGATC 1 cut(s) 776
BspT107I GGYRCC 4 cut(s) 53, 64, 408, 1463
BsrFI RCCGGY 2 cut(s) 34, 56
BsrI ACTGG 4 cut(s) 68, 671, 931, 1458
BssAI RCCGGY 2 cut(s) 34, 56
BssMI GATC 3 cut(s) 396, 781, 921
BssSI CACGAG 1 cut(s) 844
BssT1I CCWWGG 7 cut(s) 309, 423, 677, 810, 913, 1010, 1072
Bst2BI CACGAG 1 cut(s) 844
Bst2UI CCWGG 3 cut(s) 47, 335, 406
Bst4CI ACNGT 4 cut(s) 382, 550, 1296, 1329
Bst6I CTCTTC 1 cut(s) 1356
BstC8I GCNNGC 9 cut(s) 58, 137, 301, 348, 352, 356, 471, 649, 1316
BstDEI CTNAG 5 cut(s) 193, 393, 1137, 1201, 1470
BstDSI CCRYGG 4 cut(s) 423, 810, 1010, 1095
BstEII GGTNACC 1 cut(s) 1267
BstENI CCTNNNNNAGG 1 cut(s) 951
BstF5I GGATG 9 cut(s) 498, 516, 759, 764, 806, 974, 1259, 1348, 1454
BstFNI CGCG 4 cut(s) 62, 791, 1180, 1182
BstHHI GCGC 4 cut(s) 62, 839, 1182, 1314
BstKTI GATC 3 cut(s) 399, 784, 924
BstMAI GTCTC 1 cut(s) 380
BstMBI GATC 3 cut(s) 396, 781, 921
BstMCI CGRYCG 1 cut(s) 228
BstNI CCWGG 3 cut(s) 47, 335, 406
BstNSI RCATGY 1 cut(s) 1318
BstPI GGTNACC 1 cut(s) 1267
BstSCI CCNGG 7 cut(s) 45, 139, 333, 404, 638, 740, 1100
BstSFI CTRYAG 2 cut(s) 951, 978
BstSLI GKGCMC 1 cut(s) 1156
BstUI CGCG 4 cut(s) 62, 791, 1180, 1182
BstV1I GCAGC 4 cut(s) 122, 350, 461, 937
BstX2I RGATCY 1 cut(s) 781
BstXI CCANNNNNNTGG 1 cut(s) 1474
BstYI RGATCY 1 cut(s) 781
BsuRI GGCC 8 cut(s) 126, 303, 315, 794, 815, 930, 974, 1100
BtgI CCRYGG 4 cut(s) 423, 810, 1010, 1095
BtsCI GGATG 9 cut(s) 498, 516, 759, 764, 806, 974, 1259, 1348, 1454
BtsI GCAGTG 4 cut(s) 192, 657, 1009, 1356
BtsIMutI CAGTG 8 cut(s) 192, 443, 546, 657, 924, 1009, 1356, 1465
BveI ACCTGC 2 cut(s) 755, 944
Cac8I GCNNGC 9 cut(s) 58, 137, 301, 348, 352, 356, 471, 649, 1316
CaiI CAGNNNCTG 1 cut(s) 1333
CfoI GCGC 4 cut(s) 62, 839, 1182, 1314
Cfr10I RCCGGY 2 cut(s) 34, 56
Cfr13I GGNCC 4 cut(s) 302, 313, 674, 972
CseI GACGC 1 cut(s) 1355
Csp6I GTAC 7 cut(s) 43, 157, 409, 544, 719, 787, 1192
CviQI GTAC 7 cut(s) 43, 157, 409, 544, 719, 787, 1192
DdeI CTNAG 5 cut(s) 193, 393, 1137, 1201, 1470
DpnI GATC 3 cut(s) 398, 783, 923
DpnII GATC 3 cut(s) 396, 781, 921
DraIII CACNNNGTG 1 cut(s) 1096
EaeI YGGCCR 3 cut(s) 792, 813, 1098
Eam1104I CTCTTC 1 cut(s) 1356
EarI CTCTTC 1 cut(s) 1356
Eco130I CCWWGG 7 cut(s) 309, 423, 677, 810, 913, 1010, 1072
Eco24I GRGCYC 1 cut(s) 1240
Eco47I GGWCC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 349
Eco91I GGTNACC 1 cut(s) 1267
EcoNI CCTNNNNNAGG 1 cut(s) 951
EcoO65I GGTNACC 1 cut(s) 1267
EcoRII CCWGG 3 cut(s) 45, 333, 404
EcoT14I CCWWGG 7 cut(s) 309, 423, 677, 810, 913, 1010, 1072
EcoT22I ATGCAT 2 cut(s) 941, 1375
EcoT38I GRGCYC 1 cut(s) 1240
ErhI CCWWGG 7 cut(s) 309, 423, 677, 810, 913, 1010, 1072
FaqI GGGAC 2 cut(s) 101, 284
FauI CCCGC 1 cut(s) 466
FauNDI CATATG 1 cut(s) 1369
FbaI TGATCA 1 cut(s) 921
FokI GGATG 9 cut(s) 505, 523, 766, 771, 793, 981, 1266, 1355, 1441
FriOI GRGCYC 1 cut(s) 1240
FspBI CTAG 3 cut(s) 9, 1286, 1407
FspI TGCGCA 1 cut(s) 1313
GlaI GCGC 4 cut(s) 61, 838, 1181, 1313
GsaI CCCAGC 1 cut(s) 241
HaeIII GGCC 8 cut(s) 126, 303, 315, 794, 815, 930, 974, 1100
HapII CCGG 9 cut(s) 35, 57, 140, 612, 639, 735, 741, 1101, 1319
HgaI GACGC 1 cut(s) 1355
HhaI GCGC 4 cut(s) 62, 839, 1182, 1314
Hin6I GCGC 4 cut(s) 60, 837, 1180, 1312
HinP1I GCGC 4 cut(s) 60, 837, 1180, 1312
HincII GTYRAC 1 cut(s) 1063
HindII GTYRAC 1 cut(s) 1063
HinfI GANTC 2 cut(s) 803, 1258
HpaII CCGG 9 cut(s) 35, 57, 140, 612, 639, 735, 741, 1101, 1319
HphI GGTGA 5 cut(s) 646, 694, 1009, 1261, 1394
Hpy166II GTNNAC 8 cut(s) 190, 546, 1017, 1033, 1063, 1084, 1154, 1192
Hpy188I TCNGA 2 cut(s) 554, 1426
Hpy188III TCNNGA 1 cut(s) 1203
Hpy8I GTNNAC 8 cut(s) 190, 546, 1017, 1033, 1063, 1084, 1154, 1192
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 3 cut(s) 295, 682, 903
HpyCH4III ACNGT 4 cut(s) 382, 550, 1296, 1329
HpyCH4IV ACGT 2 cut(s) 717, 1433
HpyCH4V TGCA 7 cut(s) 449, 939, 953, 1154, 1213, 1354, 1373
HpyF3I CTNAG 5 cut(s) 193, 393, 1137, 1201, 1470
HpySE526I ACGT 2 cut(s) 717, 1433
HspAI GCGC 4 cut(s) 60, 837, 1180, 1312
KpnI GGTACC 1 cut(s) 412
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
Ksp22I TGATCA 1 cut(s) 921
Kzo9I GATC 3 cut(s) 396, 781, 921
LmnI GCTCC 5 cut(s) 282, 554, 737, 905, 1235
Lsp1109I GCAGC 4 cut(s) 122, 350, 461, 937
MaeI CTAG 3 cut(s) 9, 1286, 1407
MaeII ACGT 2 cut(s) 717, 1433
MaeIII GTNAC 4 cut(s) 1168, 1267, 1402, 1434
MalI GATC 3 cut(s) 398, 783, 923
MboI GATC 3 cut(s) 396, 781, 921
MboII GAAGA 4 cut(s) 627, 697, 871, 1373
MflI RGATCY 1 cut(s) 781
MhlI GDGCHC 3 cut(s) 356, 1156, 1240
MluCI AATT 1 cut(s) 1227
MlyI GAGTC 1 cut(s) 797
MmeI TCCRAC 1 cut(s) 532
Mph1103I ATGCAT 2 cut(s) 941, 1375
MroNI GCCGGC 1 cut(s) 56
MseI TTAA 1 cut(s) 1121
MslI CAYNNNNRTG 3 cut(s) 594, 1022, 1472
MspI CCGG 9 cut(s) 35, 57, 140, 612, 639, 735, 741, 1101, 1319
MspR9I CCNGG 7 cut(s) 47, 141, 335, 406, 640, 742, 1102
Mva1269I GAATGC 1 cut(s) 1215
MvaI CCWGG 3 cut(s) 47, 335, 406
MvnI CGCG 4 cut(s) 62, 791, 1180, 1182
NaeI GCCGGC 1 cut(s) 58
NciI CCSGG 4 cut(s) 141, 640, 742, 1102
NcoI CCATGG 3 cut(s) 423, 810, 1010
NdeI CATATG 1 cut(s) 1369
NdeII GATC 3 cut(s) 396, 781, 921
NgoMIV GCCGGC 1 cut(s) 56
NlaIV GGNNCC 6 cut(s) 55, 66, 410, 739, 1237, 1465
NmuCI GTSAC 3 cut(s) 1168, 1267, 1434
NsbI TGCGCA 1 cut(s) 1313
NsiI ATGCAT 2 cut(s) 941, 1375
NspI RCATGY 1 cut(s) 1318
OliI CACNNNNGTG 1 cut(s) 1472
PaeI GCATGC 1 cut(s) 1318
PctI GAATGC 1 cut(s) 1215
PdiI GCCGGC 1 cut(s) 58
PfeI GAWTC 1 cut(s) 1258
Pfl23II CGTACG 1 cut(s) 786
PflMI CCANNNNNTGG 3 cut(s) 429, 1096, 1460
PfoI TCCNGGA 1 cut(s) 638
PleI GAGTC 1 cut(s) 797
PpsI GAGTC 1 cut(s) 797
Psp6I CCWGG 3 cut(s) 45, 333, 404
PspEI GGTNACC 1 cut(s) 1267
PspFI CCCAGC 1 cut(s) 237
PspGI CCWGG 3 cut(s) 45, 333, 404
PspLI CGTACG 1 cut(s) 786
PspN4I GGNNCC 6 cut(s) 55, 66, 410, 739, 1237, 1465
PspPI GGNCC 4 cut(s) 302, 313, 674, 972
PstI CTGCAG 1 cut(s) 955
PstNI CAGNNNCTG 1 cut(s) 1333
PsuI RGATCY 1 cut(s) 781
RsaI GTAC 7 cut(s) 44, 158, 410, 545, 720, 788, 1193
RsaNI GTAC 7 cut(s) 43, 157, 409, 544, 719, 787, 1192
RseI CAYNNNNRTG 3 cut(s) 594, 1022, 1472
SaqAI TTAA 1 cut(s) 1121
Sau3AI GATC 3 cut(s) 396, 781, 921
Sau96I GGNCC 4 cut(s) 302, 313, 674, 972
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 7 cut(s) 47, 141, 335, 406, 640, 742, 1102
SduI GDGCHC 3 cut(s) 356, 1156, 1240
SfcI CTRYAG 2 cut(s) 951, 978
SinI GGWCC 1 cut(s) 674
SmiMI CAYNNNNRTG 3 cut(s) 594, 1022, 1472
SphI GCATGC 1 cut(s) 1318
Sse9I AATT 1 cut(s) 1227
SspMI CTAG 3 cut(s) 9, 1286, 1407
StyD4I CCNGG 7 cut(s) 45, 139, 333, 404, 638, 740, 1100
StyI CCWWGG 7 cut(s) 309, 423, 677, 810, 913, 1010, 1072
TaaI ACNGT 4 cut(s) 382, 550, 1296, 1329
TaiI ACGT 2 cut(s) 720, 1436
TaqI TCGA 1 cut(s) 870
TasI AATT 1 cut(s) 1227
TauI GCSGC 6 cut(s) 224, 526, 794, 842, 977, 1116
TfiI GAWTC 1 cut(s) 1258
Tru1I TTAA 1 cut(s) 1121
Tru9I TTAA 1 cut(s) 1121
TscAI CASTG 8 cut(s) 192, 443, 553, 664, 931, 1009, 1356, 1465
TseFI GTSAC 3 cut(s) 1168, 1267, 1434
TseI GCWGC 5 cut(s) 5, 110, 338, 449, 950
Tsp45I GTSAC 3 cut(s) 1168, 1267, 1434
TspDTI ATGAA 3 cut(s) 190, 509, 617
TspGWI ACGGA 6 cut(s) 174, 774, 815, 886, 1209, 1296
TspRI CASTG 8 cut(s) 192, 443, 553, 664, 931, 1009, 1356, 1465
Van91I CCANNNNNTGG 3 cut(s) 429, 1096, 1460
VneI GTGCAC 1 cut(s) 1152
VpaK11BI GGWCC 1 cut(s) 674
XagI CCTNNNNNAGG 1 cut(s) 951
XceI RCATGY 1 cut(s) 1318
XcmI CCANNNNNNNNNTGG 2 cut(s) 850, 1027
XspI CTAG 3 cut(s) 9, 1286, 1407
Zsp2I ATGCAT 2 cut(s) 941, 1375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.