RchiOBHm_Chr3g0484631

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0001358
Physical Location & Seq
Forward (+)
31025779 .. 31028070
2292 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44934

Sequence Viewer

Length: 801 bp
ATGCTGACGTTGGTCGAGTTTCAGGTAGGACCTAGGGTGCAGATGGACAGGGAGAGATTCCCACCAAACAACGTGCTGCTGACTTTGGTGGGAGCAGGGTTGCTGTGGATGGGATGGGCCGGTTTCAACGGCGGAGATCCGTATGCGGCCAACATAACCGCCTCCATGGCTGTCCTCAACACTAACATTTGCGCCGCCACGAGTCTCTTGGTGTGGACGGCGCTCGATGTCATCTTCTTCAACAAGCCCTCCGTCATCGGAGCAGTCCAGGGAATGATTACTGGCCTCGTCGTCATTACTCCCGCTGCAGGTCTTGTACAAGGATGGGCAGCAATAGTGATGGGAGTATTGGCTGGCAGCGTGCCATGGTTCACCATGATGATCGTAGACAAAAGGTGGAGACTCTTAACCGCAGTTGACGACACCCTCGGCGTCGTCCATACCCACGCAGTGGCCGGATTCCTAGGCGGCATATTGACCGGCATCTTTGCCGAGCCCCAACTATGTGCGCTCTTCTTGCAAGTCACGAACTCTCGCGGGGTAGCCTACGGGGGCATCCAAGTGTTCAAACAAATCGCAGCTGCCCTGTTCATCATCGGATGGAACATCGTGGTGACCTCAATCATTTGTGTGGCAATTCGGTTTGTGGTTCCTCTACGCATGCCGGAGGAGCAGTTGTTGATTGGAGATAACGCAGTGCATGGGGAAGAGGCCTACGCTTTGTGGGGTGATGGGGAGCTGTACGATCATCCTTCTACTATTATAAATAATAACGGACGTGTGGCTGAGGCTCCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

266

Amino Acids

28.42

Weight (kDa)

5.09

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 9 - 239 1.7e-48 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 764
Acc36I ACCTGC 1 cut(s) 299
AccB7I CCANNNNNTGG 1 cut(s) 451
AccI GTMKAC 1 cut(s) 387
AccII CGCG 1 cut(s) 537
AciI CCGC 8 cut(s) 132, 146, 159, 195, 303, 411, 468, 537
AclWI GGATC 1 cut(s) 131
AcoI YGGCCR 2 cut(s) 147, 453
AcyI GRCGYC 1 cut(s) 432
AdeI CACNNNGTG 1 cut(s) 451
AfaI GTAC 2 cut(s) 318, 743
AfiI CCNNNNNNNGG 2 cut(s) 308, 451
AflIII ACRYGT 1 cut(s) 778
AgsI TTSAA 3 cut(s) 127, 241, 568
AjiI CACGTC 1 cut(s) 779
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 2 cut(s) 581, 739
AluI AGCT 2 cut(s) 581, 739
Alw26I GTCTC 2 cut(s) 209, 394
AlwI GGATC 1 cut(s) 131
AoxI GGCC 5 cut(s) 117, 147, 283, 453, 711
ApeKI GCWGC 6 cut(s) 76, 305, 329, 357, 578, 581
AspA2I CCTAGG 2 cut(s) 32, 463
AspLEI GCGC 3 cut(s) 194, 223, 511
AspS9I GGNCC 2 cut(s) 29, 117
AsuHPI GGTGA 3 cut(s) 364, 625, 740
AvaII GGWCC 1 cut(s) 29
AvrII CCTAGG 2 cut(s) 32, 463
BanII GRGCYC 1 cut(s) 498
BauI CACGAG 1 cut(s) 199
BbvCI CCTCAGC 1 cut(s) 786
BbvI GCAGC 6 cut(s) 63, 292, 341, 369, 568, 590
BccI CCATC 7 cut(s) 37, 103, 108, 318, 334, 594, 725
BceAI ACGGC 2 cut(s) 145, 234
BciT130I CCWGG 1 cut(s) 269
BcoDI GTCTC 2 cut(s) 209, 394
BfaI CTAG 2 cut(s) 33, 464
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 299
BglI GCCNNNNNGGC 1 cut(s) 167
BisI GCNGC 9 cut(s) 77, 147, 195, 306, 330, 358, 469, 579, 582
BlnI CCTAGG 2 cut(s) 32, 463
BlsI GCNGC 9 cut(s) 78, 148, 196, 307, 331, 359, 470, 580, 583
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 1 cut(s) 29
BmgBI CACGTC 1 cut(s) 779
BmgT120I GGNCC 2 cut(s) 29, 117
BmiI GGNNCC 2 cut(s) 651, 792
BmrFI CCNGG 1 cut(s) 269
BmrI ACTGGG 1 cut(s) 789
BmsI GCATC 2 cut(s) 492, 564
BmuI ACTGGG 1 cut(s) 789
BoxI GACNNNNGTC 1 cut(s) 11
Bpu10I CCTNAGC 1 cut(s) 786
BsaHI GRCGYC 1 cut(s) 432
BsaJI CCNNGG 6 cut(s) 32, 165, 268, 365, 427, 463
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 659, 662, 689, 692
Bsc4I CCNNNNNNNGG 2 cut(s) 308, 451
Bse118I RCCGGY 2 cut(s) 119, 479
Bse1I ACTGG 2 cut(s) 286, 795
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 6 cut(s) 32, 165, 268, 365, 427, 463
BseGI GGATG 6 cut(s) 114, 119, 329, 555, 605, 748
BseLI CCNNNNNNNGG 2 cut(s) 308, 451
BseMII CTCAG 1 cut(s) 777
BseNI ACTGG 2 cut(s) 286, 795
BseRI GAGGAG 1 cut(s) 683
BseXI GCAGC 6 cut(s) 63, 292, 341, 369, 568, 590
BsgI GTGCAG 1 cut(s) 59
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 5 cut(s) 119, 149, 285, 455, 713
BsiSI CCGG 4 cut(s) 120, 456, 480, 665
BslI CCNNNNNNNGG 2 cut(s) 308, 451
BsmAI GTCTC 2 cut(s) 209, 394
BsnI GGCC 5 cut(s) 119, 149, 285, 455, 713
Bsp1286I GDGCHC 1 cut(s) 498
Bsp1407I TGTACA 1 cut(s) 316
Bsp143I GATC 3 cut(s) 136, 381, 745
Bsp19I CCATGG 2 cut(s) 165, 365
BspACI CCGC 8 cut(s) 132, 146, 159, 195, 303, 411, 468, 537
BspANI GGCC 5 cut(s) 119, 149, 285, 455, 713
BspCNI CTCAG 1 cut(s) 778
BspFNI CGCG 1 cut(s) 537
BspLI GGNNCC 2 cut(s) 651, 792
BspMAI CTGCAG 1 cut(s) 310
BspMI ACCTGC 1 cut(s) 299
BspPI GGATC 1 cut(s) 131
BspQI GCTCTTC 1 cut(s) 518
BsrFI RCCGGY 2 cut(s) 119, 479
BsrGI TGTACA 1 cut(s) 316
BsrI ACTGG 2 cut(s) 286, 795
BssAI RCCGGY 2 cut(s) 119, 479
BssECI CCNNGG 6 cut(s) 32, 165, 268, 365, 427, 463
BssMI GATC 3 cut(s) 136, 381, 745
BssNI GRCGYC 1 cut(s) 432
BssSI CACGAG 1 cut(s) 199
BssT1I CCWWGG 4 cut(s) 32, 165, 365, 463
Bst2BI CACGAG 1 cut(s) 199
Bst2UI CCWGG 1 cut(s) 269
Bst6I CTCTTC 2 cut(s) 518, 702
BstACI GRCGYC 1 cut(s) 432
BstAUI TGTACA 1 cut(s) 316
BstC8I GCNNGC 3 cut(s) 355, 362, 662
BstDEI CTNAG 1 cut(s) 786
BstDSI CCRYGG 2 cut(s) 165, 365
BstEII GGTNACC 1 cut(s) 613
BstF5I GGATG 6 cut(s) 114, 119, 329, 555, 605, 748
BstFNI CGCG 1 cut(s) 537
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 3 cut(s) 194, 223, 511
BstKTI GATC 3 cut(s) 139, 384, 748
BstMAI GTCTC 2 cut(s) 209, 394
BstMBI GATC 3 cut(s) 136, 381, 745
BstMWI GCNNNNNNNGC 3 cut(s) 167, 517, 670
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 664
BstPAI GACNNNNGTC 1 cut(s) 11
BstPI GGTNACC 1 cut(s) 613
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 306
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 6 cut(s) 63, 292, 341, 369, 568, 590
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BsuRI GGCC 5 cut(s) 119, 149, 285, 455, 713
BtgI CCRYGG 2 cut(s) 165, 365
BtrI CACGTC 1 cut(s) 779
BtsCI GGATG 6 cut(s) 114, 119, 329, 555, 605, 748
BtsI GCAGTG 2 cut(s) 456, 702
BtsIMutI CAGTG 2 cut(s) 456, 702
BveI ACCTGC 1 cut(s) 299
Cac8I GCNNGC 3 cut(s) 355, 362, 662
CfoI GCGC 3 cut(s) 194, 223, 511
Cfr10I RCCGGY 2 cut(s) 119, 479
Cfr13I GGNCC 2 cut(s) 29, 117
CseI GACGC 1 cut(s) 421
Csp6I GTAC 2 cut(s) 317, 742
CviAII CATG 5 cut(s) 166, 366, 376, 661, 701
CviQI GTAC 2 cut(s) 317, 742
DdeI CTNAG 1 cut(s) 786
DpnI GATC 3 cut(s) 138, 383, 747
DpnII GATC 3 cut(s) 136, 381, 745
DraIII CACNNNGTG 1 cut(s) 451
EaeI YGGCCR 2 cut(s) 147, 453
Eam1104I CTCTTC 2 cut(s) 518, 702
EarI CTCTTC 2 cut(s) 518, 702
EciI GGCGGA 1 cut(s) 147
Eco130I CCWWGG 4 cut(s) 32, 165, 365, 463
Eco147I AGGCCT 1 cut(s) 713
Eco24I GRGCYC 1 cut(s) 498
Eco47I GGWCC 1 cut(s) 29
Eco91I GGTNACC 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 29
EcoO65I GGTNACC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 4 cut(s) 32, 165, 365, 463
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 4 cut(s) 32, 165, 365, 463
FaeI CATG 5 cut(s) 169, 369, 379, 664, 704
FatI CATG 5 cut(s) 165, 365, 375, 660, 700
FauI CCCGC 2 cut(s) 310, 530
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 9 cut(s) 77, 147, 195, 306, 330, 358, 469, 579, 582
FokI GGATG 6 cut(s) 121, 126, 336, 542, 612, 735
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 9 cut(s) 77, 147, 195, 306, 330, 358, 469, 579, 582
FspBI CTAG 2 cut(s) 33, 464
GlaI GCGC 3 cut(s) 193, 222, 510
GluI GCNGC 9 cut(s) 77, 147, 195, 306, 330, 358, 469, 579, 582
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 5 cut(s) 119, 149, 285, 455, 713
HapII CCGG 4 cut(s) 120, 456, 480, 665
HgaI GACGC 1 cut(s) 421
HhaI GCGC 3 cut(s) 194, 223, 511
Hin1I GRCGYC 1 cut(s) 432
Hin1II CATG 5 cut(s) 169, 369, 379, 664, 704
Hin6I GCGC 3 cut(s) 192, 221, 509
HinP1I GCGC 3 cut(s) 192, 221, 509
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 4 cut(s) 57, 202, 402, 459
HpaII CCGG 4 cut(s) 120, 456, 480, 665
HphI GGTGA 3 cut(s) 364, 625, 740
Hpy166II GTNNAC 4 cut(s) 216, 372, 388, 418
Hpy188I TCNGA 2 cut(s) 260, 599
Hpy188III TCNNGA 1 cut(s) 526
Hpy8I GTNNAC 4 cut(s) 216, 372, 388, 418
Hpy99I CGWCG 2 cut(s) 293, 437
HpyAV CCTTC 1 cut(s) 762
HpyCH4IV ACGT 3 cut(s) 8, 72, 778
HpyCH4V TGCA 4 cut(s) 40, 308, 520, 700
HpyF10VI GCNNNNNNNGC 3 cut(s) 167, 517, 670
HpyF3I CTNAG 1 cut(s) 786
HpySE526I ACGT 3 cut(s) 8, 72, 778
Hsp92I GRCGYC 1 cut(s) 432
Hsp92II CATG 5 cut(s) 169, 369, 379, 664, 704
HspAI GCGC 3 cut(s) 192, 221, 509
Kzo9I GATC 3 cut(s) 136, 381, 745
LguI GCTCTTC 1 cut(s) 518
LmnI GCTCC 5 cut(s) 92, 260, 670, 736, 796
Lsp1109I GCAGC 6 cut(s) 63, 292, 341, 369, 568, 590
LweI GCATC 2 cut(s) 492, 564
MaeI CTAG 2 cut(s) 33, 464
MaeII ACGT 3 cut(s) 8, 72, 778
MaeIII GTNAC 2 cut(s) 523, 613
MalI GATC 3 cut(s) 138, 383, 747
MboI GATC 3 cut(s) 136, 381, 745
MboII GAAGA 4 cut(s) 226, 229, 505, 719
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 498
MluCI AATT 1 cut(s) 636
MlyI GAGTC 2 cut(s) 211, 396
MseI TTAA 1 cut(s) 407
MslI CAYNNNNRTG 4 cut(s) 377, 560, 611, 629
MspA1I CMGCKG 2 cut(s) 305, 581
MspI CCGG 4 cut(s) 120, 456, 480, 665
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 3 cut(s) 167, 517, 670
NcoI CCATGG 2 cut(s) 165, 365
NdeII GATC 3 cut(s) 136, 381, 745
NlaIII CATG 5 cut(s) 169, 369, 379, 664, 704
NlaIV GGNNCC 2 cut(s) 651, 792
NmeAIII GCCGAG 2 cut(s) 408, 517
NmuCI GTSAC 2 cut(s) 523, 613
NspI RCATGY 1 cut(s) 664
PaeI GCATGC 1 cut(s) 664
PceI AGGCCT 1 cut(s) 713
PciSI GCTCTTC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 57, 459
PflMI CCANNNNNTGG 1 cut(s) 451
PkrI GCNGC 9 cut(s) 78, 148, 196, 307, 331, 359, 470, 580, 583
PleI GAGTC 2 cut(s) 210, 396
PpsI GAGTC 2 cut(s) 210, 396
PpuMI RGGWCCY 1 cut(s) 29
PshAI GACNNNNGTC 1 cut(s) 11
PsiI TTATAA 1 cut(s) 764
Psp5II RGGWCCY 1 cut(s) 29
Psp6I CCWGG 1 cut(s) 267
PspEI GGTNACC 1 cut(s) 613
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 2 cut(s) 651, 792
PspPI GGNCC 2 cut(s) 29, 117
PspPPI RGGWCCY 1 cut(s) 29
PstI CTGCAG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 136
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 2 cut(s) 318, 743
RsaNI GTAC 2 cut(s) 317, 742
RseI CAYNNNNRTG 4 cut(s) 377, 560, 611, 629
SapI GCTCTTC 1 cut(s) 518
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 9 cut(s) 77, 147, 195, 306, 330, 358, 469, 579, 582
Sau3AI GATC 3 cut(s) 136, 381, 745
Sau96I GGNCC 2 cut(s) 29, 117
SchI GAGTC 2 cut(s) 211, 396
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 1 cut(s) 498
SfaNI GCATC 2 cut(s) 492, 564
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 29
SmiMI CAYNNNNRTG 4 cut(s) 377, 560, 611, 629
SphI GCATGC 1 cut(s) 664
Sse9I AATT 1 cut(s) 636
SseBI AGGCCT 1 cut(s) 713
SsiI CCGC 8 cut(s) 132, 146, 159, 195, 303, 411, 468, 537
SspMI CTAG 2 cut(s) 33, 464
StuI AGGCCT 1 cut(s) 713
StyD4I CCNGG 1 cut(s) 267
StyI CCWWGG 4 cut(s) 32, 165, 365, 463
TaiI ACGT 3 cut(s) 11, 75, 781
TaqI TCGA 2 cut(s) 15, 225
TasI AATT 1 cut(s) 636
TatI WGTACW 1 cut(s) 316
TauI GCSGC 3 cut(s) 149, 197, 471
TfiI GAWTC 2 cut(s) 57, 459
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TscAI CASTG 2 cut(s) 456, 702
TseFI GTSAC 2 cut(s) 523, 613
TseI GCWGC 6 cut(s) 76, 305, 329, 357, 578, 581
Tsp45I GTSAC 2 cut(s) 523, 613
TspDTI ATGAA 1 cut(s) 580
TspGWI ACGGA 3 cut(s) 129, 241, 789
TspRI CASTG 2 cut(s) 456, 702
Van91I CCANNNNNTGG 1 cut(s) 451
VpaK11BI GGWCC 1 cut(s) 29
XceI RCATGY 1 cut(s) 664
XcmI CCANNNNNNNNNTGG 1 cut(s) 205
XmaJI CCTAGG 2 cut(s) 32, 463
XmiI GTMKAC 1 cut(s) 387
XspI CTAG 2 cut(s) 33, 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.