Rroxscaffold_6G00397590

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001358
Physical Location & Seq
Reverse (-)
19287808 .. 19294010
6203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397590.1

Sequence Viewer

Length: 1548 bp
ATGGCGGCCCCGGCGTACCAGGCGACTGGCGGAGCGGTGCCGGACTGGCTGAACAAAGGTGACAACGCATGGCAGATGATCTCCGCCACGCTGGTGGGTCTCCAGAGCGTGCCGGGGCTGGTCATCCTCTACGGGAGCATAGTGAAGAAGAAGTGGGCGGTCAACTCCGCCTTCATGGCCCTCTATGCCTTCGCCGCCGTCGTGCTTTGCTGGGTGTCGTGGGCCTACAAGATGTCGTTCGGGGACAAGCTGCTGCCGTTTTGGGGAAAGGCCGGGCCTGCCTTAGGGCAGAAGTTTTTGCTCAAGCACGCTGCCATCCCCGAATCGACACAGTACCGCCACAACCACAGTCTTGAGAGTCCCATGATCATGCCGTTGTACCCGATGGCGACCATGGTGTGGTTCCAGTGCGTGTTCGCGGCCATAACACTGATACTGTTGGCAGGGTCGGTGCTGGGGAGAATGAACTTCAAGGCGTGGATGATGTTCGTGCCTCTGTGGCTGACGTTCTCGTACACCATCGGTGCTTTTAGCTTGTGGGGTGGAGGGTTCTTGTTCCACTGGGGTGTTATGGACTACTCCGGCGGGTACGTCATTCATCTTTCTTCAGGGATTGCTGGTTTCACCACTGCTTACTGGAAGACAGTGAGTTATGCTTTATGCATGTGTGCTATTAGTGAATCTTTTGCAGTGGGTGTTAATGACAATGCCATTGAGGAATGTGGTTGTATTGGACAAATCATGGTAGGACCTAGGGTGAAGAAGGACAGGGAGAGATTCCCACCAAACAACGTACTGCTGACGTTGGCCGGAGCAGGGTTGCTGTGGATGGGATGGGCCGGTTTCAACGGCGGAGATCCGTACGCGGCCAACACAGACTCCTCCATGGCTGTCCTCAACACTAACATCTGCGCTGCCACGAGTCTCTTGGTGTGGACATGGCTCGATGTCATCTTCTTCAACAAGCCCTCCGTCATCGGAGCTGTCCAGGGAATGATTACTGGCCTCGTCTGTATTACTCCTGCTGCAGGTCTTGTACAAGGATGGGCAGCAGCAGTGATGGGAGTATTGTCTGGCAGCGTGCCATGGTTCACGATGATGATCGTAGACAAAAGGTGGAGACTCTTAACCGTCGTTGACGACACCCTTGGCGTTGTCCACACCCACGCAGTGGCCGGATACCTAGGCGGCATATTGACCGGCATCTTTGCCGAGCCCCAACTATGCGCACTCTTCTTACCTGTCACGAACTCTCGCGGGGTAGTCTACGGGGGCATGCAAGTGTTCAAACAAATCGTAGGCGGCCTGTTCATCATCGGATGGAACATCGTGGTGACCTCGATCATTTGTGTGGCAATCCGGTTTGTGATTCCTCTACGGATGCCAGAGGAGCAGTTGTTGATTGGAGATGATGCAGTGCATGGGGAAGAGGCCTATGCCTTGTGGGGTGATGGGGAGTTGTATGATGAAACTAAGCATGATAAACATTACTCGGATGATGCCTCACAGGGTAAGTTTGCATCCAGTGGTGCCACTCAGGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

515

Amino Acids

55.78

Weight (kDa)

6.36

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 23 - 212 4.6e-26 Ammonium Transporter Family
Ammonium_transp PF00909 245 - 479 7.8e-47 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1228
Acc36I ACCTGC 1 cut(s) 1019
AccB1I GGYRCC 2 cut(s) 37, 1529
AccB7I CCANNNNNTGG 2 cut(s) 399, 1171
AccBSI CCGCTC 1 cut(s) 35
AccI GTMKAC 2 cut(s) 1107, 1266
AccII CGCG 3 cut(s) 419, 866, 1257
AclWI GGATC 1 cut(s) 851
AcoI YGGCCR 4 cut(s) 420, 807, 867, 1173
AcuI CTGAAG 1 cut(s) 591
AdeI CACNNNGTG 1 cut(s) 1171
AfaI GTAC 8 cut(s) 17, 335, 380, 515, 590, 795, 863, 1038
AfiI CCNNNNNNNGG 6 cut(s) 263, 284, 399, 816, 1028, 1171
AgsI TTSAA 4 cut(s) 472, 847, 961, 1288
AjnI CCWGG 2 cut(s) 18, 987
AleI CACNNNNGTG 2 cut(s) 92, 564
AluBI AGCT 3 cut(s) 250, 534, 983
AluI AGCT 3 cut(s) 250, 534, 983
Alw26I GTCTC 3 cut(s) 104, 929, 1114
AlwI GGATC 1 cut(s) 851
ApeKI GCWGC 8 cut(s) 250, 253, 311, 914, 1025, 1049, 1052, 1077
AspA2I CCTAGG 2 cut(s) 752, 1183
AspLEI GCGC 2 cut(s) 914, 1229
AspS9I GGNCC 6 cut(s) 7, 178, 222, 275, 749, 837
AsuC2I CCSGG 3 cut(s) 11, 114, 274
AsuHPI GGTGA 5 cut(s) 71, 616, 769, 1345, 1460
AvaII GGWCC 1 cut(s) 749
AvrII CCTAGG 2 cut(s) 752, 1183
AxyI CCTNAGG 1 cut(s) 283
BanI GGYRCC 2 cut(s) 37, 1529
BanII GRGCYC 1 cut(s) 1218
BauI CACGAG 1 cut(s) 919
BbsI GAAGAC 1 cut(s) 647
BbvI GCAGC 8 cut(s) 237, 240, 298, 901, 1012, 1061, 1064, 1089
BccI CCATC 9 cut(s) 323, 379, 527, 823, 828, 1038, 1054, 1314, 1445
BceAI ACGGC 4 cut(s) 182, 241, 358, 865
BciT130I CCWGG 2 cut(s) 20, 989
BciVI GTATCC 1 cut(s) 1172
BclI TGATCA 1 cut(s) 366
BcnI CCSGG 3 cut(s) 11, 114, 274
BcoDI GTCTC 3 cut(s) 104, 929, 1114
BfaI CTAG 2 cut(s) 753, 1184
BfmI CTRYAG 1 cut(s) 1026
BfuAI ACCTGC 1 cut(s) 1019
BfuI GTATCC 1 cut(s) 1172
BglI GCCNNNNNGGC 3 cut(s) 46, 176, 499
BlnI CCTAGG 2 cut(s) 752, 1183
Bme1390I CCNGG 5 cut(s) 11, 20, 114, 274, 989
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 6 cut(s) 7, 178, 222, 275, 749, 837
BmiI GGNNCC 4 cut(s) 9, 39, 404, 1531
BmrFI CCNGG 5 cut(s) 11, 20, 114, 274, 989
BmrI ACTGGG 1 cut(s) 571
BmsI GCATC 5 cut(s) 1212, 1371, 1402, 1489, 1529
BmuI ACTGGG 1 cut(s) 571
BpiI GAAGAC 1 cut(s) 647
BpmI CTGGAG 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 287, 374
BpuMI CCSGG 3 cut(s) 11, 114, 274
BsaBI GATNNNNATC 1 cut(s) 1100
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 9 cut(s) 9, 113, 393, 752, 885, 988, 1085, 1147, 1183
BsaWI WCCGGW 1 cut(s) 1359
BsaXI ACNNNNNCTCC 6 cut(s) 863, 893, 953, 983, 1382, 1412
Bsc4I CCNNNNNNNGG 6 cut(s) 263, 284, 399, 816, 1028, 1171
Bse118I RCCGGY 2 cut(s) 839, 1199
Bse1I ACTGG 7 cut(s) 31, 50, 406, 566, 641, 1006, 1524
Bse21I CCTNAGG 1 cut(s) 283
Bse8I GATNNNNATC 1 cut(s) 1100
BseBI CCWGG 2 cut(s) 20, 989
BseDI CCNNGG 9 cut(s) 9, 113, 393, 752, 885, 988, 1085, 1147, 1183
BseJI GATNNNNATC 1 cut(s) 1100
BseLI CCNNNNNNNGG 6 cut(s) 263, 284, 399, 816, 1028, 1171
BseNI ACTGG 7 cut(s) 31, 50, 406, 566, 641, 1006, 1524
BseRI GAGGAG 2 cut(s) 871, 1403
BseXI GCAGC 8 cut(s) 237, 240, 298, 901, 1012, 1061, 1064, 1089
BseYI CCCAGC 2 cut(s) 210, 454
Bsh1236I CGCG 3 cut(s) 419, 866, 1257
BshNI GGYRCC 2 cut(s) 37, 1529
BsiWI CGTACG 1 cut(s) 861
BslFI GGGAC 2 cut(s) 257, 345
BslI CCNNNNNNNGG 6 cut(s) 263, 284, 399, 816, 1028, 1171
BsmAI GTCTC 3 cut(s) 104, 929, 1114
BsmFI GGGAC 2 cut(s) 257, 345
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 1218
Bsp1407I TGTACA 1 cut(s) 1036
Bsp143I GATC 5 cut(s) 78, 366, 856, 1101, 1341
Bsp19I CCATGG 3 cut(s) 393, 885, 1085
BspFNI CGCG 3 cut(s) 419, 866, 1257
BspLI GGNNCC 4 cut(s) 9, 39, 404, 1531
BspMAI CTGCAG 1 cut(s) 1030
BspMI ACCTGC 1 cut(s) 1019
BspPI GGATC 1 cut(s) 851
BspT107I GGYRCC 2 cut(s) 37, 1529
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 839, 1199
BsrGI TGTACA 1 cut(s) 1036
BsrI ACTGG 7 cut(s) 31, 50, 406, 566, 641, 1006, 1524
BssAI RCCGGY 2 cut(s) 839, 1199
BssECI CCNNGG 9 cut(s) 9, 113, 393, 752, 885, 988, 1085, 1147, 1183
BssMI GATC 5 cut(s) 78, 366, 856, 1101, 1341
BssSI CACGAG 1 cut(s) 919
BssT1I CCWWGG 6 cut(s) 393, 752, 885, 1085, 1147, 1183
Bst2BI CACGAG 1 cut(s) 919
Bst2UI CCWGG 2 cut(s) 20, 989
Bst4CI ACNGT 5 cut(s) 333, 350, 438, 646, 1132
Bst6I CTCTTC 2 cut(s) 1238, 1422
BstAUI TGTACA 1 cut(s) 1036
BstC8I GCNNGC 5 cut(s) 110, 279, 309, 1082, 1277
BstDEI CTNAG 3 cut(s) 283, 1473, 1536
BstDSI CCRYGG 3 cut(s) 393, 885, 1085
BstEII GGTNACC 1 cut(s) 1333
BstENI CCTNNNNNAGG 2 cut(s) 282, 1026
BstFNI CGCG 3 cut(s) 419, 866, 1257
BstHHI GCGC 2 cut(s) 914, 1229
BstKTI GATC 5 cut(s) 81, 369, 859, 1104, 1344
BstMAI GTCTC 3 cut(s) 104, 929, 1114
BstMBI GATC 5 cut(s) 78, 366, 856, 1101, 1341
BstMWI GCNNNNNNNGC 9 cut(s) 11, 20, 46, 176, 185, 194, 278, 499, 1390
BstNI CCWGG 2 cut(s) 20, 989
BstNSI RCATGY 2 cut(s) 667, 1279
BstPI GGTNACC 1 cut(s) 1333
BstSCI CCNGG 5 cut(s) 9, 18, 112, 272, 987
BstSFI CTRYAG 1 cut(s) 1026
BstUI CGCG 3 cut(s) 419, 866, 1257
BstV1I GCAGC 8 cut(s) 237, 240, 298, 901, 1012, 1061, 1064, 1089
BstV2I GAAGAC 1 cut(s) 647
BstX2I RGATCY 1 cut(s) 856
BstXI CCANNNNNNTGG 2 cut(s) 26, 94
BstYI RGATCY 1 cut(s) 856
Bsu36I CCTNAGG 1 cut(s) 283
BsuI GTATCC 1 cut(s) 1172
BtgI CCRYGG 3 cut(s) 393, 885, 1085
BtsI GCAGTG 5 cut(s) 627, 696, 1062, 1176, 1422
BveI ACCTGC 1 cut(s) 1019
Cac8I GCNNGC 5 cut(s) 110, 279, 309, 1082, 1277
CfoI GCGC 2 cut(s) 914, 1229
Cfr10I RCCGGY 2 cut(s) 839, 1199
Cfr13I GGNCC 6 cut(s) 7, 178, 222, 275, 749, 837
Csp6I GTAC 8 cut(s) 16, 334, 379, 514, 589, 794, 862, 1037
CspCI CAANNNNNGTGG 1 cut(s) 1522
CviQI GTAC 8 cut(s) 16, 334, 379, 514, 589, 794, 862, 1037
DdeI CTNAG 3 cut(s) 283, 1473, 1536
DpnI GATC 5 cut(s) 80, 368, 858, 1103, 1343
DpnII GATC 5 cut(s) 78, 366, 856, 1101, 1341
DraIII CACNNNGTG 1 cut(s) 1171
EaeI YGGCCR 4 cut(s) 420, 807, 867, 1173
Eam1104I CTCTTC 2 cut(s) 1238, 1422
EarI CTCTTC 2 cut(s) 1238, 1422
EciI GGCGGA 4 cut(s) 45, 73, 157, 867
Eco130I CCWWGG 6 cut(s) 393, 752, 885, 1085, 1147, 1183
Eco147I AGGCCT 1 cut(s) 1433
Eco24I GRGCYC 1 cut(s) 1218
Eco31I GGTCTC 1 cut(s) 104
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 591
Eco81I CCTNAGG 1 cut(s) 283
Eco91I GGTNACC 1 cut(s) 1333
EcoNI CCTNNNNNAGG 2 cut(s) 282, 1026
EcoO109I RGGNCCY 1 cut(s) 749
EcoO65I GGTNACC 1 cut(s) 1333
EcoRII CCWGG 2 cut(s) 18, 987
EcoT14I CCWWGG 6 cut(s) 393, 752, 885, 1085, 1147, 1183
EcoT22I ATGCAT 1 cut(s) 665
EcoT38I GRGCYC 1 cut(s) 1218
ErhI CCWWGG 6 cut(s) 393, 752, 885, 1085, 1147, 1183
FaqI GGGAC 2 cut(s) 257, 345
FauI CCCGC 2 cut(s) 578, 1250
FbaI TGATCA 1 cut(s) 366
FblI GTMKAC 2 cut(s) 1107, 1266
FriOI GRGCYC 1 cut(s) 1218
FspAI RTGCGCAY 1 cut(s) 1228
FspBI CTAG 2 cut(s) 753, 1184
FspI TGCGCA 1 cut(s) 1228
GlaI GCGC 2 cut(s) 913, 1228
GsaI CCCAGC 2 cut(s) 214, 458
GsuI CTGGAG 1 cut(s) 86
HhaI GCGC 2 cut(s) 914, 1229
Hin6I GCGC 2 cut(s) 912, 1227
HinP1I GCGC 2 cut(s) 912, 1227
HincII GTYRAC 2 cut(s) 163, 1138
HindII GTYRAC 2 cut(s) 163, 1138
HinfI GANTC 8 cut(s) 323, 358, 680, 777, 878, 922, 1122, 1369
HphI GGTGA 5 cut(s) 71, 616, 769, 1345, 1460
Hpy166II GTNNAC 8 cut(s) 163, 516, 936, 1092, 1108, 1138, 1159, 1267
Hpy188I TCNGA 3 cut(s) 980, 1319, 1495
Hpy188III TCNNGA 4 cut(s) 103, 353, 1093, 1246
Hpy8I GTNNAC 8 cut(s) 163, 516, 936, 1092, 1108, 1138, 1159, 1267
Hpy99I CGWCG 2 cut(s) 203, 1136
HpyAV CCTTC 3 cut(s) 181, 199, 757
HpyCH4III ACNGT 5 cut(s) 333, 350, 438, 646, 1132
HpyCH4IV ACGT 4 cut(s) 506, 591, 792, 803
HpyCH4V TGCA 7 cut(s) 663, 689, 1028, 1279, 1415, 1420, 1520
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 20, 46, 176, 185, 194, 278, 499, 1390
HpyF3I CTNAG 3 cut(s) 283, 1473, 1536
HpySE526I ACGT 4 cut(s) 506, 591, 792, 803
HspAI GCGC 2 cut(s) 912, 1227
Ksp22I TGATCA 1 cut(s) 366
Kzo9I GATC 5 cut(s) 78, 366, 856, 1101, 1341
LmnI GCTCC 5 cut(s) 32, 135, 812, 980, 1390
Lsp1109I GCAGC 8 cut(s) 237, 240, 298, 901, 1012, 1061, 1064, 1089
LweI GCATC 5 cut(s) 1212, 1371, 1402, 1489, 1529
MaeI CTAG 2 cut(s) 753, 1184
MaeII ACGT 4 cut(s) 506, 591, 792, 803
MaeIII GTNAC 3 cut(s) 59, 1243, 1333
MalI GATC 5 cut(s) 80, 368, 858, 1103, 1343
MbiI CCGCTC 1 cut(s) 35
MboI GATC 5 cut(s) 78, 366, 856, 1101, 1341
MboII GAAGA 9 cut(s) 157, 160, 597, 652, 772, 946, 949, 1225, 1439
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 1 cut(s) 1218
MlyI GAGTC 4 cut(s) 367, 872, 931, 1116
Mph1103I ATGCAT 1 cut(s) 665
MseI TTAA 2 cut(s) 699, 1127
MslI CAYNNNNRTG 7 cut(s) 92, 368, 564, 1097, 1280, 1331, 1349
MspR9I CCNGG 5 cut(s) 11, 20, 114, 274, 989
MvaI CCWGG 2 cut(s) 20, 989
MvnI CGCG 3 cut(s) 419, 866, 1257
MwoI GCNNNNNNNGC 9 cut(s) 11, 20, 46, 176, 185, 194, 278, 499, 1390
NciI CCSGG 3 cut(s) 11, 114, 274
NcoI CCATGG 3 cut(s) 393, 885, 1085
NdeII GATC 5 cut(s) 78, 366, 856, 1101, 1341
NlaIV GGNNCC 4 cut(s) 9, 39, 404, 1531
NmeAIII GCCGAG 1 cut(s) 1237
NmuCI GTSAC 3 cut(s) 59, 1243, 1333
NsbI TGCGCA 1 cut(s) 1228
NsiI ATGCAT 1 cut(s) 665
NspI RCATGY 2 cut(s) 667, 1279
OliI CACNNNNGTG 2 cut(s) 92, 564
PaeI GCATGC 1 cut(s) 1279
PceI AGGCCT 1 cut(s) 1433
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PfeI GAWTC 4 cut(s) 323, 680, 777, 1369
Pfl23II CGTACG 1 cut(s) 861
PflMI CCANNNNNTGG 2 cut(s) 399, 1171
PleI GAGTC 4 cut(s) 366, 872, 930, 1116
PpsI GAGTC 4 cut(s) 366, 872, 930, 1116
PpuMI RGGWCCY 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 749
Psp6I CCWGG 2 cut(s) 18, 987
PspEI GGTNACC 1 cut(s) 1333
PspFI CCCAGC 2 cut(s) 210, 454
PspGI CCWGG 2 cut(s) 18, 987
PspLI CGTACG 1 cut(s) 861
PspN4I GGNNCC 4 cut(s) 9, 39, 404, 1531
PspPI GGNCC 6 cut(s) 7, 178, 222, 275, 749, 837
PspPPI RGGWCCY 1 cut(s) 749
PstI CTGCAG 1 cut(s) 1030
PsuI RGATCY 1 cut(s) 856
RsaI GTAC 8 cut(s) 17, 335, 380, 515, 590, 795, 863, 1038
RsaNI GTAC 8 cut(s) 16, 334, 379, 514, 589, 794, 862, 1037
RseI CAYNNNNRTG 7 cut(s) 92, 368, 564, 1097, 1280, 1331, 1349
SaqAI TTAA 2 cut(s) 699, 1127
Sau3AI GATC 5 cut(s) 78, 366, 856, 1101, 1341
Sau96I GGNCC 6 cut(s) 7, 178, 222, 275, 749, 837
SchI GAGTC 4 cut(s) 367, 872, 931, 1116
ScrFI CCNGG 5 cut(s) 11, 20, 114, 274, 989
SduI GDGCHC 1 cut(s) 1218
SfaNI GCATC 5 cut(s) 1212, 1371, 1402, 1489, 1529
SfcI CTRYAG 1 cut(s) 1026
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 7 cut(s) 92, 368, 564, 1097, 1280, 1331, 1349
SmlI CTYRAG 2 cut(s) 302, 353
SmoI CTYRAG 2 cut(s) 302, 353
SphI GCATGC 1 cut(s) 1279
SseBI AGGCCT 1 cut(s) 1433
SspMI CTAG 2 cut(s) 753, 1184
StuI AGGCCT 1 cut(s) 1433
StyD4I CCNGG 5 cut(s) 9, 18, 112, 272, 987
StyI CCWWGG 6 cut(s) 393, 752, 885, 1085, 1147, 1183
TaaI ACNGT 5 cut(s) 333, 350, 438, 646, 1132
TaiI ACGT 4 cut(s) 509, 594, 795, 806
TaqI TCGA 3 cut(s) 326, 945, 1340
TatI WGTACW 1 cut(s) 1036
TauI GCSGC 6 cut(s) 8, 197, 422, 869, 1191, 1305
TfiI GAWTC 4 cut(s) 323, 680, 777, 1369
Tru1I TTAA 2 cut(s) 699, 1127
Tru9I TTAA 2 cut(s) 699, 1127
TseFI GTSAC 3 cut(s) 59, 1243, 1333
TseI GCWGC 8 cut(s) 250, 253, 311, 914, 1025, 1049, 1052, 1077
Tsp45I GTSAC 3 cut(s) 59, 1243, 1333
TspDTI ATGAA 5 cut(s) 163, 479, 587, 1300, 1482
TspGWI ACGGA 3 cut(s) 849, 961, 1393
Van91I CCANNNNNTGG 2 cut(s) 399, 1171
VpaK11BI GGWCC 1 cut(s) 749
XagI CCTNNNNNAGG 2 cut(s) 282, 1026
XceI RCATGY 2 cut(s) 667, 1279
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XmaJI CCTAGG 2 cut(s) 752, 1183
XmiI GTMKAC 2 cut(s) 1107, 1266
XspI CTAG 2 cut(s) 753, 1184
Zsp2I ATGCAT 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.