Rmu_sc0007199.1_g000001

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007199.1
OG0001358
Physical Location & Seq
Reverse (-)
1 .. 2893
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007199.1_g000001.1.cds

Sequence Viewer

Length: 919 bp
atggcggcgtacgaggcaactggcggagcgaacccggactggctgaacaaaggtgacaacgcatggcagatgacctccgccacgctggtgggtctccagagcgtaccggggctcgtcatcctctacgggagcatagtgaagaagaagtgggcggtaaactccgctttcatggccctctacgccttcgccgccgtcttgatctgctgggtgacgtgggcctacaagatgtcgttcggggagaagctgctgccgttttggggcaaggccgggcctgccttagggcagaagtttctgctcaagcaggctgccatccccgcctccacacactactacgccaacggcgatgtcgagactgccatggccacgccgtggtacccgatggcgagcatggtgtattttcactgcatgtttgcggccataacaccgatactgttggcagggtcggtgctggggagaatgaacttcaaggcgtggatgatgttcgtgcctctgtggctgaccttctcgtacaccgtcggtgcgtttagcatgtggggcggagggtttttatttcattggggagttatggactacgccggcgggtacgtcattcatctttcttcagggaccgctggtttcatcactgcttactgggtaggacctaggctgaagaaggacagggagagattcccaccaaacaacgtgctgctgactttggcgggagcggggttgctgtggatgggatgggccggtttcaacggcggagatccgtatgcggccaacataacagcctccatggctgtcctcaacactaacatctgcgccgccacgagtctcgtggtgtggacggcgctcgatgtcatcttcttcaacaagccctccgtcatcggagcagtccagggaatgattaccggcctcgtcgtcattactcccgctgcag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

306

Amino Acids

33.09

Weight (kDa)

9.15

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 372
AccB7I CCANNNNNTGG 1 cut(s) 369
AccBSI CCGCTC 1 cut(s) 704
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 3 cut(s) 360, 414, 756
AcuI CTGAAG 2 cut(s) 585, 668
AdeI CACNNNGTG 1 cut(s) 369
AfaI GTAC 5 cut(s) 11, 105, 374, 509, 584
AfiI CCNNNNNNNGG 3 cut(s) 257, 278, 369
AgsI TTSAA 3 cut(s) 466, 736, 850
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 1 cut(s) 876
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 1 cut(s) 244
AluI AGCT 1 cut(s) 244
Alw26I GTCTC 3 cut(s) 98, 344, 818
AlwI GGATC 1 cut(s) 740
AoxI GGCC 9 cut(s) 171, 216, 264, 269, 360, 414, 726, 756, 892
ApeKI GCWGC 5 cut(s) 244, 247, 305, 685, 914
Asp718I GGTACC 1 cut(s) 372
AspA2I CCTAGG 1 cut(s) 641
AspLEI GCGC 2 cut(s) 803, 832
AspS9I GGNCC 6 cut(s) 172, 216, 269, 606, 638, 726
AsuC2I CCSGG 3 cut(s) 35, 108, 268
AsuHPI GGTGA 2 cut(s) 65, 220
AvaII GGWCC 2 cut(s) 606, 638
AvrII CCTAGG 1 cut(s) 641
AxyI CCTNAGG 1 cut(s) 277
BalI TGGCCA 1 cut(s) 362
BanI GGYRCC 1 cut(s) 372
BanII GRGCYC 1 cut(s) 114
BauI CACGAG 2 cut(s) 808, 815
BbvI GCAGC 5 cut(s) 231, 234, 292, 672, 901
BccI CCATC 4 cut(s) 317, 373, 712, 717
BceAI ACGGC 6 cut(s) 176, 235, 352, 355, 754, 843
BciT130I CCWGG 1 cut(s) 878
BcnI CCSGG 3 cut(s) 35, 108, 268
BcoDI GTCTC 3 cut(s) 98, 344, 818
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 1 cut(s) 915
BfoI RGCGCY 1 cut(s) 833
BglI GCCNNNNNGGC 2 cut(s) 493, 776
BlnI CCTAGG 1 cut(s) 641
Bme1390I CCNGG 4 cut(s) 35, 108, 268, 878
Bme18I GGWCC 2 cut(s) 606, 638
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 6 cut(s) 172, 216, 269, 606, 638, 726
BmiI GGNNCC 2 cut(s) 374, 607
BmrFI CCNGG 4 cut(s) 35, 108, 268, 878
BmrI ACTGGG 1 cut(s) 640
BmuI ACTGGG 1 cut(s) 640
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 3 cut(s) 35, 108, 268
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 6 cut(s) 107, 357, 368, 641, 774, 877
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 3 cut(s) 257, 278, 369
Bse118I RCCGGY 3 cut(s) 575, 728, 890
Bse1I ACTGG 3 cut(s) 25, 44, 635
Bse21I CCTNAGG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 878
BseDI CCNNGG 6 cut(s) 107, 357, 368, 641, 774, 877
BseGI GGATG 5 cut(s) 117, 309, 480, 723, 728
BseLI CCNNNNNNNGG 3 cut(s) 257, 278, 369
BseNI ACTGG 3 cut(s) 25, 44, 635
BseXI GCAGC 5 cut(s) 231, 234, 292, 672, 901
BseYI CCCAGC 2 cut(s) 204, 448
BshFI GGCC 9 cut(s) 173, 218, 266, 271, 362, 416, 728, 758, 894
BshNI GGYRCC 1 cut(s) 372
BsiSI CCGG 6 cut(s) 35, 107, 267, 576, 729, 891
BsiWI CGTACG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 3 cut(s) 257, 278, 369
BsmAI GTCTC 3 cut(s) 98, 344, 818
BsmFI GGGAC 1 cut(s) 619
BsnI GGCC 9 cut(s) 173, 218, 266, 271, 362, 416, 728, 758, 894
Bso31I GGTCTC 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 2 cut(s) 198, 745
Bsp19I CCATGG 2 cut(s) 357, 774
BspANI GGCC 9 cut(s) 173, 218, 266, 271, 362, 416, 728, 758, 894
BspLI GGNNCC 2 cut(s) 374, 607
BspMAI CTGCAG 1 cut(s) 919
BspPI GGATC 1 cut(s) 740
BspT107I GGYRCC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 98
BsrBI CCGCTC 1 cut(s) 704
BsrFI RCCGGY 3 cut(s) 575, 728, 890
BsrI ACTGG 3 cut(s) 25, 44, 635
BssAI RCCGGY 3 cut(s) 575, 728, 890
BssECI CCNNGG 6 cut(s) 107, 357, 368, 641, 774, 877
BssMI GATC 2 cut(s) 198, 745
BssSI CACGAG 2 cut(s) 808, 815
BssT1I CCWWGG 3 cut(s) 357, 641, 774
Bst2BI CACGAG 2 cut(s) 808, 815
Bst2UI CCWGG 1 cut(s) 878
Bst4CI ACNGT 2 cut(s) 432, 514
BstC8I GCNNGC 4 cut(s) 273, 303, 385, 577
BstDEI CTNAG 1 cut(s) 277
BstDSI CCRYGG 3 cut(s) 357, 368, 774
BstENI CCTNNNNNAGG 1 cut(s) 276
BstF5I GGATG 5 cut(s) 117, 309, 480, 723, 728
BstH2I RGCGCY 1 cut(s) 833
BstHHI GCGC 2 cut(s) 803, 832
BstKTI GATC 2 cut(s) 201, 748
BstMAI GTCTC 3 cut(s) 98, 344, 818
BstMBI GATC 2 cut(s) 198, 745
BstMWI GCNNNNNNNGC 9 cut(s) 14, 170, 179, 188, 272, 314, 493, 534, 776
BstNI CCWGG 1 cut(s) 878
BstNSI RCATGY 2 cut(s) 409, 532
BstSCI CCNGG 4 cut(s) 33, 106, 266, 876
BstSFI CTRYAG 1 cut(s) 915
BstV1I GCAGC 5 cut(s) 231, 234, 292, 672, 901
BstX2I RGATCY 1 cut(s) 745
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 745
Bsu36I CCTNAGG 1 cut(s) 277
BsuRI GGCC 9 cut(s) 173, 218, 266, 271, 362, 416, 728, 758, 894
BtgI CCRYGG 3 cut(s) 357, 368, 774
BtgZI GCGATG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 5 cut(s) 117, 309, 480, 723, 728
BtsI GCAGTG 2 cut(s) 400, 621
BtsIMutI CAGTG 2 cut(s) 400, 621
Cac8I GCNNGC 4 cut(s) 273, 303, 385, 577
CfoI GCGC 2 cut(s) 803, 832
Cfr10I RCCGGY 3 cut(s) 575, 728, 890
Cfr13I GGNCC 6 cut(s) 172, 216, 269, 606, 638, 726
Csp6I GTAC 5 cut(s) 10, 104, 373, 508, 583
CviAII CATG 7 cut(s) 63, 169, 358, 388, 406, 529, 775
CviQI GTAC 5 cut(s) 10, 104, 373, 508, 583
DdeI CTNAG 1 cut(s) 277
DpnI GATC 2 cut(s) 200, 747
DpnII GATC 2 cut(s) 198, 745
DraIII CACNNNGTG 1 cut(s) 369
EaeI YGGCCR 3 cut(s) 360, 414, 756
EciI GGCGGA 4 cut(s) 39, 67, 552, 756
Eco130I CCWWGG 3 cut(s) 357, 641, 774
Eco24I GRGCYC 1 cut(s) 114
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 2 cut(s) 606, 638
Eco57I CTGAAG 2 cut(s) 585, 668
Eco81I CCTNAGG 1 cut(s) 277
EcoNI CCTNNNNNAGG 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 876
EcoT14I CCWWGG 3 cut(s) 357, 641, 774
EcoT38I GRGCYC 1 cut(s) 114
ErhI CCWWGG 3 cut(s) 357, 641, 774
FaeI CATG 7 cut(s) 66, 172, 361, 391, 409, 532, 778
FaqI GGGAC 1 cut(s) 619
FatI CATG 7 cut(s) 62, 168, 357, 387, 405, 528, 774
FauI CCCGC 4 cut(s) 322, 572, 691, 697
FokI GGATG 5 cut(s) 104, 296, 487, 730, 735
FriOI GRGCYC 1 cut(s) 114
FspBI CTAG 1 cut(s) 642
GlaI GCGC 2 cut(s) 802, 831
GsaI CCCAGC 2 cut(s) 208, 452
GsuI CTGGAG 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 833
HaeIII GGCC 9 cut(s) 173, 218, 266, 271, 362, 416, 728, 758, 894
HapII CCGG 6 cut(s) 35, 107, 267, 576, 729, 891
HhaI GCGC 2 cut(s) 803, 832
Hin1II CATG 7 cut(s) 66, 172, 361, 391, 409, 532, 778
Hin6I GCGC 2 cut(s) 801, 830
HinP1I GCGC 2 cut(s) 801, 830
HinfI GANTC 2 cut(s) 666, 811
HpaII CCGG 6 cut(s) 35, 107, 267, 576, 729, 891
HphI GGTGA 2 cut(s) 65, 220
Hpy166II GTNNAC 3 cut(s) 157, 510, 825
Hpy188I TCNGA 1 cut(s) 869
Hpy188III TCNNGA 3 cut(s) 97, 196, 349
Hpy8I GTNNAC 3 cut(s) 157, 510, 825
Hpy99I CGWCG 2 cut(s) 518, 902
HpyAV CCTTC 3 cut(s) 193, 511, 646
HpyCH4III ACNGT 2 cut(s) 432, 514
HpyCH4IV ACGT 3 cut(s) 212, 585, 681
HpyCH4V TGCA 2 cut(s) 405, 917
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 170, 179, 188, 272, 314, 493, 534, 776
HpyF3I CTNAG 1 cut(s) 277
HpySE526I ACGT 3 cut(s) 212, 585, 681
Hsp92II CATG 7 cut(s) 66, 172, 361, 391, 409, 532, 778
HspAI GCGC 2 cut(s) 801, 830
KpnI GGTACC 1 cut(s) 376
KroI GCCGGC 1 cut(s) 575
KroNI GCCGGC 1 cut(s) 577
Kzo9I GATC 2 cut(s) 198, 745
LmnI GCTCC 4 cut(s) 26, 129, 701, 869
Lsp1109I GCAGC 5 cut(s) 231, 234, 292, 672, 901
MaeI CTAG 1 cut(s) 642
MaeII ACGT 3 cut(s) 212, 585, 681
MaeIII GTNAC 2 cut(s) 53, 208
MalI GATC 2 cut(s) 200, 747
MbiI CCGCTC 1 cut(s) 704
MboI GATC 2 cut(s) 198, 745
MboII GAAGA 6 cut(s) 151, 154, 591, 661, 835, 838
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 362
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 1 cut(s) 820
Mox20I TGGCCA 1 cut(s) 362
MreI CGCCGGCG 1 cut(s) 575
MroNI GCCGGC 1 cut(s) 575
MscI TGGCCA 1 cut(s) 362
MslI CAYNNNNRTG 1 cut(s) 86
Msp20I TGGCCA 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 611, 914
MspI CCGG 6 cut(s) 35, 107, 267, 576, 729, 891
MspR9I CCNGG 4 cut(s) 35, 108, 268, 878
MvaI CCWGG 1 cut(s) 878
MwoI GCNNNNNNNGC 9 cut(s) 14, 170, 179, 188, 272, 314, 493, 534, 776
NaeI GCCGGC 1 cut(s) 577
NciI CCSGG 3 cut(s) 35, 108, 268
NcoI CCATGG 2 cut(s) 357, 774
NdeII GATC 2 cut(s) 198, 745
NgoMIV GCCGGC 1 cut(s) 575
NlaIII CATG 7 cut(s) 66, 172, 361, 391, 409, 532, 778
NlaIV GGNNCC 2 cut(s) 374, 607
NmuCI GTSAC 2 cut(s) 53, 208
NspI RCATGY 2 cut(s) 409, 532
OliI CACNNNNGTG 1 cut(s) 86
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 345
PdiI GCCGGC 1 cut(s) 577
PfeI GAWTC 1 cut(s) 666
Pfl23II CGTACG 1 cut(s) 9
PflMI CCANNNNNTGG 1 cut(s) 369
PleI GAGTC 1 cut(s) 819
PpsI GAGTC 1 cut(s) 819
PpuMI RGGWCCY 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 876
PspFI CCCAGC 2 cut(s) 204, 448
PspGI CCWGG 1 cut(s) 876
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 374, 607
PspPI GGNCC 6 cut(s) 172, 216, 269, 606, 638, 726
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 1 cut(s) 745
RsaI GTAC 5 cut(s) 11, 105, 374, 509, 584
RsaNI GTAC 5 cut(s) 10, 104, 373, 508, 583
RseI CAYNNNNRTG 1 cut(s) 86
Sau3AI GATC 2 cut(s) 198, 745
Sau96I GGNCC 6 cut(s) 172, 216, 269, 606, 638, 726
SchI GAGTC 1 cut(s) 820
ScrFI CCNGG 4 cut(s) 35, 108, 268, 878
SduI GDGCHC 1 cut(s) 114
SetI ASST 8 cut(s) 55, 77, 215, 246, 503, 588, 643, 684
SfcI CTRYAG 1 cut(s) 915
SgrAI CRCCGGYG 1 cut(s) 575
SinI GGWCC 2 cut(s) 606, 638
SmiMI CAYNNNNRTG 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 4 cut(s) 33, 106, 266, 876
StyI CCWWGG 3 cut(s) 357, 641, 774
TaaI ACNGT 2 cut(s) 432, 514
TaiI ACGT 3 cut(s) 215, 588, 684
TaqI TCGA 2 cut(s) 348, 834
TauI GCSGC 5 cut(s) 8, 191, 416, 758, 806
TfiI GAWTC 1 cut(s) 666
TscAI CASTG 2 cut(s) 407, 628
TseFI GTSAC 2 cut(s) 53, 208
TseI GCWGC 5 cut(s) 244, 247, 305, 685, 914
Tsp45I GTSAC 2 cut(s) 53, 208
TspDTI ATGAA 5 cut(s) 157, 473, 542, 581, 607
TspGWI ACGGA 2 cut(s) 738, 850
TspRI CASTG 2 cut(s) 407, 628
Van91I CCANNNNNTGG 1 cut(s) 369
VpaK11BI GGWCC 2 cut(s) 606, 638
XagI CCTNNNNNAGG 1 cut(s) 276
XceI RCATGY 2 cut(s) 409, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 814
XmaJI CCTAGG 1 cut(s) 641
XspI CTAG 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.