Rorug03G0212700

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001358
Physical Location & Seq
Reverse (-)
19161456 .. 19162557
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0212700.1

Sequence Viewer

Length: 684 bp
ATGTCAGAGAAACAAGCCCACTTGAATGGAGCCTATTACGGCCCATCAATCCCGCCAACAAAAGACTACCACCGCCCCGGCCGCGGCGGAGGTCCCATTGGCTGCTGCTGCAGCTGCATCTTCGGCCTCGTCTTCAAGCTCATCTTCACCGCAGTCGTCTTCATGGGATTAGCCTTCCTTGTCTTCTGGCTCATAGTCAGGCCAACCAGAGTCAAGTTTCACGTCACCGATGCCACGCTCACGCAATTCAACTTCTCCACAGATAGCAACCTGCACTACAACCTTGCCCTCAACCTCACCATCCGAAACCCCAACAAGAAGATCGGTGTCTACTACGATCGCATTGAAGCCAGAGCTCTTTACGAGGGCCAGAGGTTTAGTACGGTCACCCTCACGCCATTTTACCAAGGCCACAAAACCACAAACGTTTTGAACCCGGTGTTTCAGGGACAGCAATTGATTGTTGGATCCAACTTGCTTGATGAGTTTAATCAACAGAAGAGTGCAGGGGTTTACGAGATTGAGATGAAGCTTTATCTGAGGATTCGGTTCAAGTTGGGTAGAATCAAGACCGGAAAGTTCAAGCCTAGAATTGAGTGTGACTTGAAGGTTCCTTTGAACCAGAATGGGAATTCAGTTGGTACTTTTCAGACTGAGAAGTGCTCCATTAATTACTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

227

Amino Acids

25.8

Weight (kDa)

9.58

Isoelectric Point (pI)

29.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 194 1.9e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccI GTMKAC 1 cut(s) 330
AccII CGCG 1 cut(s) 84
AciI CCGC 6 cut(s) 53, 73, 82, 84, 87, 150
AclI AACGTT 1 cut(s) 426
AclWI GGATC 2 cut(s) 462, 475
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 631
AfaI GTAC 2 cut(s) 382, 643
AfiI CCNNNNNNNGG 1 cut(s) 83
AjiI CACGTC 1 cut(s) 223
AluBI AGCT 4 cut(s) 114, 139, 356, 532
AluI AGCT 4 cut(s) 114, 139, 356, 532
Alw21I GWGCWC 2 cut(s) 358, 665
AlwI GGATC 2 cut(s) 462, 475
AoxI GGCC 6 cut(s) 40, 79, 124, 200, 367, 409
ApeKI GCWGC 5 cut(s) 102, 105, 108, 111, 114
ApoI RAATTY 1 cut(s) 631
AseI ATTAAT 1 cut(s) 669
AspS9I GGNCC 3 cut(s) 41, 92, 367
AsuC2I CCSGG 2 cut(s) 78, 437
AsuHPI GGTGA 4 cut(s) 139, 217, 289, 379
AvaII GGWCC 1 cut(s) 92
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 3 cut(s) 124, 151, 175
Bbv12I GWGCWC 2 cut(s) 358, 665
BbvI GCAGC 5 cut(s) 89, 92, 95, 101, 123
BccI CCATC 2 cut(s) 52, 308
BceAI ACGGC 1 cut(s) 55
BcnI CCSGG 2 cut(s) 78, 437
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 279
BisI GCNGC 7 cut(s) 82, 85, 103, 106, 109, 112, 115
BlsI GCNGC 7 cut(s) 83, 86, 104, 107, 110, 113, 116
Bme1390I CCNGG 2 cut(s) 78, 437
Bme18I GGWCC 1 cut(s) 92
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 41, 92, 367
BmiI GGNNCC 4 cut(s) 31, 94, 469, 612
BmrFI CCNGG 2 cut(s) 78, 437
BmsI GCATC 2 cut(s) 126, 220
BpiI GAAGAC 3 cut(s) 124, 151, 175
BplI GAGNNNNNCTC 2 cut(s) 647, 679
BpuMI CCSGG 2 cut(s) 78, 437
BsaJI CCNNGG 3 cut(s) 76, 82, 406
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseDI CCNNGG 3 cut(s) 76, 82, 406
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 2 cut(s) 530, 645
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 5 cut(s) 89, 92, 95, 101, 123
BsgI GTGCAG 2 cut(s) 257, 525
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 2 cut(s) 82, 340
BshFI GGCC 6 cut(s) 42, 81, 126, 202, 369, 411
BsiEI CGRYCG 2 cut(s) 82, 340
BsiHKAI GWGCWC 2 cut(s) 358, 665
BsiSI CCGG 3 cut(s) 78, 437, 573
BslFI GGGAC 2 cut(s) 78, 462
BslI CCNNNNNNNGG 1 cut(s) 83
BsmFI GGGAC 2 cut(s) 78, 462
BsnI GGCC 6 cut(s) 42, 81, 126, 202, 369, 411
Bsp1286I GDGCHC 2 cut(s) 358, 665
Bsp143I GATC 3 cut(s) 321, 337, 467
BspACI CCGC 6 cut(s) 53, 73, 82, 84, 87, 150
BspANI GGCC 6 cut(s) 42, 81, 126, 202, 369, 411
BspCNI CTCAG 2 cut(s) 531, 646
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 4 cut(s) 31, 94, 469, 612
BspMAI CTGCAG 1 cut(s) 113
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 2 cut(s) 462, 475
BssECI CCNNGG 3 cut(s) 76, 82, 406
BssMI GATC 3 cut(s) 321, 337, 467
BssT1I CCWWGG 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 494
BstDEI CTNAG 2 cut(s) 539, 654
BstDSI CCRYGG 1 cut(s) 82
BstEII GGTNACC 1 cut(s) 385
BstF5I GGATG 1 cut(s) 300
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 3 cut(s) 324, 340, 470
BstMBI GATC 3 cut(s) 321, 337, 467
BstMCI CGRYCG 2 cut(s) 82, 340
BstMWI GCNNNNNNNGC 5 cut(s) 81, 108, 111, 114, 123
BstPI GGTNACC 1 cut(s) 385
BstSCI CCNGG 2 cut(s) 76, 435
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 89, 92, 95, 101, 123
BstV2I GAAGAC 3 cut(s) 124, 151, 175
BstX2I RGATCY 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 1 cut(s) 467
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 6 cut(s) 42, 81, 126, 202, 369, 411
BtgI CCRYGG 1 cut(s) 82
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 279
Cfr13I GGNCC 3 cut(s) 41, 92, 367
Cfr42I CCGCGG 1 cut(s) 85
Csp6I GTAC 2 cut(s) 381, 642
CviAII CATG 1 cut(s) 163
CviQI GTAC 2 cut(s) 381, 642
DdeI CTNAG 2 cut(s) 539, 654
DpnI GATC 3 cut(s) 323, 339, 469
DpnII GATC 3 cut(s) 321, 337, 467
EaeI YGGCCR 1 cut(s) 79
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 92
Eco52I CGGCCG 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 356
Eco91I GGTNACC 1 cut(s) 385
EcoICRI GAGCTC 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 92
EcoO65I GGTNACC 1 cut(s) 385
EcoRI GAATTC 1 cut(s) 631
EcoT14I CCWWGG 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 1 cut(s) 166
FaiI YATR 2 cut(s) 164, 194
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 2 cut(s) 78, 462
FatI CATG 1 cut(s) 162
FauI CCCGC 1 cut(s) 60
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 7 cut(s) 82, 85, 103, 106, 109, 112, 115
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 7 cut(s) 82, 85, 103, 106, 109, 112, 115
FspBI CTAG 1 cut(s) 588
GluI GCNGC 7 cut(s) 82, 85, 103, 106, 109, 112, 115
HaeIII GGCC 6 cut(s) 42, 81, 126, 202, 369, 411
HapII CCGG 3 cut(s) 78, 437, 573
Hin1II CATG 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 3 cut(s) 210, 544, 564
HpaII CCGG 3 cut(s) 78, 437, 573
HphI GGTGA 4 cut(s) 139, 217, 289, 379
Hpy166II GTNNAC 2 cut(s) 331, 514
Hpy188I TCNGA 4 cut(s) 7, 305, 540, 651
Hpy188III TCNNGA 1 cut(s) 568
Hpy8I GTNNAC 2 cut(s) 331, 514
HpyAV CCTTC 2 cut(s) 184, 601
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 222, 426
HpyCH4V TGCA 4 cut(s) 111, 117, 274, 506
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 108, 111, 114, 123
HpyF3I CTNAG 2 cut(s) 539, 654
HpySE526I ACGT 2 cut(s) 222, 426
Hsp92II CATG 1 cut(s) 166
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 3 cut(s) 321, 337, 467
LmnI GCTCC 2 cut(s) 29, 668
Lsp1109I GCAGC 5 cut(s) 89, 92, 95, 101, 123
LweI GCATC 2 cut(s) 126, 220
MaeI CTAG 1 cut(s) 588
MaeII ACGT 2 cut(s) 222, 426
MaeIII GTNAC 3 cut(s) 223, 385, 599
MalI GATC 3 cut(s) 323, 339, 469
MboI GATC 3 cut(s) 321, 337, 467
MboII GAAGA 7 cut(s) 112, 124, 136, 151, 175, 331, 511
MfeI CAATTG 2 cut(s) 455, 679
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 2 cut(s) 358, 665
MluCI AATT 6 cut(s) 245, 455, 591, 631, 670, 679
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 2 cut(s) 445, 495
MnlI CCTC 8 cut(s) 83, 137, 299, 305, 358, 366, 401, 534
MseI TTAA 2 cut(s) 489, 669
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 2 cut(s) 84, 114
MspI CCGG 3 cut(s) 78, 437, 573
MspR9I CCNGG 2 cut(s) 78, 437
MunI CAATTG 2 cut(s) 455, 679
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 5 cut(s) 81, 108, 111, 114, 123
NciI CCSGG 2 cut(s) 78, 437
NdeII GATC 3 cut(s) 321, 337, 467
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 4 cut(s) 31, 94, 469, 612
NmuCI GTSAC 3 cut(s) 223, 385, 599
PfeI GAWTC 2 cut(s) 544, 564
PkrI GCNGC 7 cut(s) 83, 86, 104, 107, 110, 113, 116
Ple19I CGATCG 1 cut(s) 340
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PpuMI RGGWCCY 1 cut(s) 92
PshBI ATTAAT 1 cut(s) 669
Psp124BI GAGCTC 1 cut(s) 358
Psp1406I AACGTT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 92
PspEI GGTNACC 1 cut(s) 385
PspN4I GGNNCC 4 cut(s) 31, 94, 469, 612
PspPI GGNCC 3 cut(s) 41, 92, 367
PspPPI RGGWCCY 1 cut(s) 92
PstI CTGCAG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 467
PvuI CGATCG 1 cut(s) 340
PvuII CAGCTG 1 cut(s) 114
RsaI GTAC 2 cut(s) 382, 643
RsaNI GTAC 2 cut(s) 381, 642
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 358
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 2 cut(s) 489, 669
SatI GCNGC 7 cut(s) 82, 85, 103, 106, 109, 112, 115
Sau3AI GATC 3 cut(s) 321, 337, 467
Sau96I GGNCC 3 cut(s) 41, 92, 367
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 2 cut(s) 78, 437
SduI GDGCHC 2 cut(s) 358, 665
SfaNI GCATC 2 cut(s) 126, 220
SfcI CTRYAG 1 cut(s) 109
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 24
Sse9I AATT 6 cut(s) 245, 455, 591, 631, 670, 679
SsiI CCGC 6 cut(s) 53, 73, 82, 84, 87, 150
SspMI CTAG 1 cut(s) 588
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 76, 435
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 225, 429
TasI AATT 6 cut(s) 245, 455, 591, 631, 670, 679
TauI GCSGC 2 cut(s) 84, 87
TfiI GAWTC 2 cut(s) 544, 564
Tru1I TTAA 2 cut(s) 489, 669
Tru9I TTAA 2 cut(s) 489, 669
TseFI GTSAC 3 cut(s) 223, 385, 599
TseI GCWGC 5 cut(s) 102, 105, 108, 111, 114
Tsp45I GTSAC 3 cut(s) 223, 385, 599
TspDTI ATGAA 2 cut(s) 151, 542
VpaK11BI GGWCC 1 cut(s) 92
VspI ATTAAT 1 cut(s) 669
XapI RAATTY 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.