Rorug03G0212800

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001358
Physical Location & Seq
Reverse (-)
19176931 .. 19178027
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0212800.1

Sequence Viewer

Length: 684 bp
ATGTCAGAGAAACAAGCCCACTTGAATGGAGCCTATTACGGCCCATCAATCCCGCCGAGAGAGAACTACCACCGCCCCGGCCGCGGCGGAGGTCCCCTCAGCTGCTGCTGTAGCTGCATCTTCGGTCTCGTCTTCAAGCTCATCTTCTCCGCAGTTGTCTTCATGGGGTTAGCCTTCCTCGTCTTCTGGCTCATAGTCCGCCCCACCAGAGTCAAGTTCCACGTAACAGATGCCACTCTCACACAATTCAACTTCTCCACCGATAGCAACCTGCACTACAATCTCGCTCTCAACCTCACCATCCGAAACCCCAACAAGAAAATCGGCGTTTACTATGATCGCATAGAAGCCAGAGCTCTTTACGAGGACCAGAGGTTTAGTACGGTCACTCTGACCCCATTTTACCAAGGCCACCAAACCACAAACGTTTTGAACCCGGTGTTTCAGGGACAGCAATTGATTGTTGGATCCAACTTTCTTGAGGAGTTTAATCAACAGAAGAGTGCTGGAGTTTACGAGATCGAGATGAAGCTTTATCTGAGGATTCGGTTCAAGTTGGGTAGAATCAAGACCGGAAAGTTCAAGCCTAGAATTGAATGTGACTTCAAGGTTCCTTTGAGTCAGAATGGAAATTCAGTTAGTACTTTTCAGACTGAGAAATGCTCAATTGATTACTTCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

227

Amino Acids

26.01

Weight (kDa)

9.39

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 99 - 195 1e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccII CGCG 1 cut(s) 84
AciI CCGC 7 cut(s) 53, 73, 82, 84, 87, 150, 199
AclI AACGTT 1 cut(s) 426
AclWI GGATC 2 cut(s) 462, 475
AcoI YGGCCR 1 cut(s) 79
AcsI RAATTY 1 cut(s) 631
AfaI GTAC 2 cut(s) 382, 643
AfiI CCNNNNNNNGG 1 cut(s) 83
AgsI TTSAA 9 cut(s) 25, 136, 250, 433, 553, 583, 596, 607, 679
AluBI AGCT 5 cut(s) 102, 114, 139, 356, 532
AluI AGCT 5 cut(s) 102, 114, 139, 356, 532
Alw21I GWGCWC 1 cut(s) 358
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 2 cut(s) 462, 475
AlwNI CAGNNNCTG 1 cut(s) 105
AoxI GGCC 3 cut(s) 40, 79, 409
ApeKI GCWGC 3 cut(s) 102, 105, 114
ApoI RAATTY 1 cut(s) 631
AspS9I GGNCC 3 cut(s) 41, 92, 367
AsuC2I CCSGG 2 cut(s) 78, 437
AsuHPI GGTGA 1 cut(s) 289
AvaII GGWCC 2 cut(s) 92, 367
BamHI GGATCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 3 cut(s) 124, 151, 175
Bbv12I GWGCWC 1 cut(s) 358
BbvCI CCTCAGC 1 cut(s) 98
BbvI GCAGC 3 cut(s) 89, 92, 101
BccI CCATC 2 cut(s) 52, 308
BceAI ACGGC 1 cut(s) 55
BcnI CCSGG 2 cut(s) 78, 437
BcoDI GTCTC 1 cut(s) 131
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 279
BisI GCNGC 5 cut(s) 82, 85, 103, 106, 115
BlsI GCNGC 5 cut(s) 83, 86, 104, 107, 116
BmcAI AGTACT 1 cut(s) 643
Bme1390I CCNGG 2 cut(s) 78, 437
Bme18I GGWCC 2 cut(s) 92, 367
BmgT120I GGNCC 3 cut(s) 41, 92, 367
BmiI GGNNCC 4 cut(s) 31, 94, 469, 612
BmrFI CCNGG 2 cut(s) 78, 437
BmsI GCATC 2 cut(s) 126, 220
BpiI GAAGAC 3 cut(s) 124, 151, 175
BplI GAGNNNNNCTC 4 cut(s) 81, 113, 647, 679
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 1 cut(s) 98
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 2 cut(s) 78, 437
BsaAI YACGTR 1 cut(s) 223
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 76, 82, 406
BsaWI WCCGGW 1 cut(s) 572
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseDI CCNNGG 3 cut(s) 76, 82, 406
BseGI GGATG 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 83
BseMII CTCAG 3 cut(s) 112, 530, 645
BseRI GAGGAG 1 cut(s) 497
BseX3I CGGCCG 1 cut(s) 79
BseXI GCAGC 3 cut(s) 89, 92, 101
BsgI GTGCAG 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 82
BshFI GGCC 3 cut(s) 42, 81, 411
BsiEI CGRYCG 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 358
BsiSI CCGG 3 cut(s) 78, 437, 573
BslFI GGGAC 2 cut(s) 78, 462
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 131
BsmFI GGGAC 2 cut(s) 78, 462
BsnI GGCC 3 cut(s) 42, 81, 411
Bso31I GGTCTC 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 3 cut(s) 337, 467, 519
BspACI CCGC 7 cut(s) 53, 73, 82, 84, 87, 150, 199
BspANI GGCC 3 cut(s) 42, 81, 411
BspCNI CTCAG 3 cut(s) 111, 531, 646
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 4 cut(s) 31, 94, 469, 612
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 2 cut(s) 462, 475
BspTNI GGTCTC 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 76, 82, 406
BssMI GATC 3 cut(s) 337, 467, 519
BssT1I CCWWGG 1 cut(s) 406
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 1 cut(s) 494
BstBAI YACGTR 1 cut(s) 223
BstDEI CTNAG 3 cut(s) 98, 539, 654
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 300
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 3 cut(s) 340, 470, 522
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 337, 467, 519
BstMCI CGRYCG 1 cut(s) 82
BstMWI GCNNNNNNNGC 3 cut(s) 81, 111, 114
BstSCI CCNGG 2 cut(s) 76, 435
BstSFI CTRYAG 1 cut(s) 109
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 3 cut(s) 89, 92, 101
BstV2I GAAGAC 3 cut(s) 124, 151, 175
BstX2I RGATCY 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 26
BstYI RGATCY 1 cut(s) 467
BstZI CGGCCG 1 cut(s) 79
BsuRI GGCC 3 cut(s) 42, 81, 411
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 1 cut(s) 300
BveI ACCTGC 1 cut(s) 279
CaiI CAGNNNCTG 1 cut(s) 105
Cfr13I GGNCC 3 cut(s) 41, 92, 367
Cfr42I CCGCGG 1 cut(s) 85
Csp6I GTAC 2 cut(s) 381, 642
CviAII CATG 1 cut(s) 163
CviQI GTAC 2 cut(s) 381, 642
DdeI CTNAG 3 cut(s) 98, 539, 654
DpnI GATC 3 cut(s) 339, 469, 521
DpnII GATC 3 cut(s) 337, 467, 519
EaeI YGGCCR 1 cut(s) 79
EagI CGGCCG 1 cut(s) 79
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 2 cut(s) 102, 188
Ecl136II GAGCTC 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 358
Eco31I GGTCTC 1 cut(s) 131
Eco47I GGWCC 2 cut(s) 92, 367
Eco52I CGGCCG 1 cut(s) 79
Eco53kI GAGCTC 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 356
EcoO109I RGGNCCY 1 cut(s) 92
EcoT14I CCWWGG 1 cut(s) 406
EcoT38I GRGCYC 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 1 cut(s) 166
FaiI YATR 4 cut(s) 164, 194, 336, 344
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 2 cut(s) 78, 462
FatI CATG 1 cut(s) 162
FauI CCCGC 1 cut(s) 60
Fnu4HI GCNGC 5 cut(s) 82, 85, 103, 106, 115
FokI GGATG 1 cut(s) 287
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 5 cut(s) 82, 85, 103, 106, 115
FspBI CTAG 1 cut(s) 588
GluI GCNGC 5 cut(s) 82, 85, 103, 106, 115
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 3 cut(s) 42, 81, 411
HapII CCGG 3 cut(s) 78, 437, 573
Hin1II CATG 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 4 cut(s) 210, 544, 564, 619
HpaII CCGG 3 cut(s) 78, 437, 573
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 2 cut(s) 331, 514
Hpy188I TCNGA 6 cut(s) 7, 305, 393, 540, 624, 651
Hpy188III TCNNGA 3 cut(s) 479, 523, 568
Hpy8I GTNNAC 2 cut(s) 331, 514
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 2 cut(s) 222, 426
HpyCH4V TGCA 2 cut(s) 117, 274
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 111, 114
HpyF3I CTNAG 3 cut(s) 98, 539, 654
HpySE526I ACGT 2 cut(s) 222, 426
Hsp92II CATG 1 cut(s) 166
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 3 cut(s) 337, 467, 519
LmnI GCTCC 1 cut(s) 29
Lsp1109I GCAGC 3 cut(s) 89, 92, 101
LweI GCATC 2 cut(s) 126, 220
MaeI CTAG 1 cut(s) 588
MaeII ACGT 2 cut(s) 222, 426
MaeIII GTNAC 3 cut(s) 223, 385, 599
MalI GATC 3 cut(s) 339, 469, 521
MboI GATC 3 cut(s) 337, 467, 519
MboII GAAGA 6 cut(s) 112, 124, 136, 151, 175, 511
MfeI CAATTG 3 cut(s) 455, 666, 679
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 6 cut(s) 245, 455, 591, 631, 666, 679
MlyI GAGTC 2 cut(s) 219, 628
MmeI TCCRAC 2 cut(s) 445, 495
MnlI CCTC 8 cut(s) 83, 107, 188, 305, 358, 366, 475, 534
MseI TTAA 1 cut(s) 489
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 2 cut(s) 84, 102
MspI CCGG 3 cut(s) 78, 437, 573
MspR9I CCNGG 2 cut(s) 78, 437
MunI CAATTG 3 cut(s) 455, 666, 679
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 81, 111, 114
NciI CCSGG 2 cut(s) 78, 437
NdeII GATC 3 cut(s) 337, 467, 519
NlaIII CATG 1 cut(s) 166
NlaIV GGNNCC 4 cut(s) 31, 94, 469, 612
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 2 cut(s) 385, 599
PfeI GAWTC 2 cut(s) 544, 564
PkrI GCNGC 5 cut(s) 83, 86, 104, 107, 116
PleI GAGTC 2 cut(s) 218, 627
PpsI GAGTC 2 cut(s) 218, 627
Ppu21I YACGTR 1 cut(s) 223
PpuMI RGGWCCY 1 cut(s) 92
Psp124BI GAGCTC 1 cut(s) 358
Psp1406I AACGTT 1 cut(s) 426
Psp5II RGGWCCY 1 cut(s) 92
PspN4I GGNNCC 4 cut(s) 31, 94, 469, 612
PspPI GGNCC 3 cut(s) 41, 92, 367
PspPPI RGGWCCY 1 cut(s) 92
PstNI CAGNNNCTG 1 cut(s) 105
PsuI RGATCY 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 102
RsaI GTAC 2 cut(s) 382, 643
RsaNI GTAC 2 cut(s) 381, 642
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 358
SacII CCGCGG 1 cut(s) 85
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 5 cut(s) 82, 85, 103, 106, 115
Sau3AI GATC 3 cut(s) 337, 467, 519
Sau96I GGNCC 3 cut(s) 41, 92, 367
ScaI AGTACT 1 cut(s) 643
SchI GAGTC 2 cut(s) 219, 628
ScrFI CCNGG 2 cut(s) 78, 437
SduI GDGCHC 1 cut(s) 358
SfaNI GCATC 2 cut(s) 126, 220
SfcI CTRYAG 1 cut(s) 109
Sfr303I CCGCGG 1 cut(s) 85
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 2 cut(s) 92, 367
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 6 cut(s) 245, 455, 591, 631, 666, 679
SsiI CCGC 7 cut(s) 53, 73, 82, 84, 87, 150, 199
SspMI CTAG 1 cut(s) 588
SstI GAGCTC 1 cut(s) 358
StyD4I CCNGG 2 cut(s) 76, 435
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 2 cut(s) 225, 429
TaqI TCGA 1 cut(s) 522
TaqII GACCGA 1 cut(s) 113
TasI AATT 6 cut(s) 245, 455, 591, 631, 666, 679
TatI WGTACW 1 cut(s) 641
TauI GCSGC 2 cut(s) 84, 87
TfiI GAWTC 2 cut(s) 544, 564
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 2 cut(s) 385, 599
TseI GCWGC 3 cut(s) 102, 105, 114
Tsp45I GTSAC 2 cut(s) 385, 599
TspDTI ATGAA 2 cut(s) 151, 542
VpaK11BI GGWCC 2 cut(s) 92, 367
XapI RAATTY 1 cut(s) 631
XspI CTAG 1 cut(s) 588
ZrmI AGTACT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.