Rh3CG295600

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0001358
Physical Location & Seq
Forward (+)
30222794 .. 30231202
8409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG295600.1

Sequence Viewer

Length: 1245 bp
ATGGCGGCGTACGAGGCAACTGGCGGAGCGAACCCGGACTGGCTGAACAAAGGTGACAACGCATGGCAGATGACCTCTGCCACGCTGGTGGGTCTCCAGAGCATGCCGGGGCTCGTCATCCTCTACGGGAGCATAGTGAAGAAGAAGTGGGCGGTAAACTCTGCTTTCATGGCCCTCTACGCCTTCGCCGCCGTCGTGATCTGCTGGGTGACGTGGGCCTACAAGATGTCGTTCGGGGAGAAGCTGCTGCCGTTTTGGGGCAAGGCCGGGCCTGCTTTAGGGCAGAAGTTTCTGCTCAAGCAGGCTGCCATCCCCGCCTCCACACACTACCACGCCAACGGCGATCTCGAGACTGCCATGGCCACGCCGTGGTACCCGATGGCGAGCATGGTGTACTTTCACTGCATGTTTGCGGCCATAACACCGATACTGTTGGCAGGGTCGGTGCTGGGGAGAATGAACTTCAAGGCGTGGATGATGTTCGTGCCTCTGTGGCTGACCTTCTCGTACACCGTCGGTGCGTTTAGCATGTGGGGCGGAGGGTTTTTATTTCATTGGGGAGTTATGGACTACGCCGGCGGGTACGTCATTCATCTTTCTTCAGGGACCGCTGGTTTCATCACTGCTTACTGGGTAGGACCTAGGGCGCAGATGGACAGGGAGAGATTCCCACCAAACAACGTGCTGCTGACTTTGGCGGGAGCAGGGTTGCTGTGGATGGGATGGGCTGGTTTCAACGGCGGAGATCCGTATGCGGCCAACATAACCGCCTCCATGGCTGTCCTCAACACTAACATCTGCGCCGCCACGAGTCTCTTGGTGTGGACGGCGCTCGATGTCATCTTCTTCAACAAGCCCTCCGTCATCGGAGCAGTCCAGGGAATGATTACCGGCCTCGTCGTCATTACTCCCGCTGCAGGTCTTGTACAAGGATGGGCAGCAATAGTGATGGGAGTATTGGCTGGCAGCGTGCCATGGTTCACCATGATGATCGTAGACAAAAGGTGGAGACTCTTAACCGCAGTTGACGACACCCTCGGCGTCGTGCATACCCACGCAGTGGCCGGATTCCTAGGCGGCATATTGACCGGTATCTTTGCCGAGCCCCAACTATGTGCGCTCTTCTTACCGGCCACGAACTCTCGCGGGGTAGCCTACGGGGGCATCCAAGTGTTCAAACAAATCGTAGCCGCCCTGTTCATCATCGGATGGAACATCGTGCACAATCCATTATCAAGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ammonium_transp

Protein Analysis

414

Amino Acids

44.58

Weight (kDa)

9.08

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 21 - 407 1.1e-70 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 908
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 372
AccB7I CCANNNNNTGG 2 cut(s) 369, 1060
AccI GTMKAC 1 cut(s) 996
AccII CGCG 1 cut(s) 1146
AclWI GGATC 1 cut(s) 740
AcoI YGGCCR 5 cut(s) 360, 414, 756, 1062, 1131
AcuI CTGAAG 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 1041
AdeI CACNNNGTG 2 cut(s) 369, 1060
AfaI GTAC 6 cut(s) 11, 374, 395, 509, 584, 927
AfiI CCNNNNNNNGG 5 cut(s) 257, 278, 369, 917, 1060
AgeI ACCGGT 1 cut(s) 1088
AgsI TTSAA 4 cut(s) 466, 736, 850, 1177
AjiI CACGTC 1 cut(s) 213
AjnI CCWGG 1 cut(s) 876
AleI CACNNNNGTG 1 cut(s) 86
AluBI AGCT 1 cut(s) 244
AluI AGCT 1 cut(s) 244
Alw21I GWGCWC 1 cut(s) 1224
Alw26I GTCTC 4 cut(s) 98, 344, 818, 1003
Alw44I GTGCAC 1 cut(s) 1220
AlwI GGATC 1 cut(s) 740
Ama87I CYCGRG 1 cut(s) 347
ApaLI GTGCAC 1 cut(s) 1220
ApeKI GCWGC 7 cut(s) 244, 247, 305, 685, 914, 938, 966
AsiGI ACCGGT 1 cut(s) 1088
Asp718I GGTACC 1 cut(s) 372
AspA2I CCTAGG 2 cut(s) 641, 1072
AspLEI GCGC 4 cut(s) 649, 803, 832, 1120
AspS9I GGNCC 5 cut(s) 172, 216, 269, 606, 638
AsuC2I CCSGG 3 cut(s) 35, 108, 268
AsuHPI GGTGA 3 cut(s) 65, 220, 973
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 2 cut(s) 606, 638
AvrII CCTAGG 2 cut(s) 641, 1072
BaeGI GKGCMC 1 cut(s) 1224
BalI TGGCCA 1 cut(s) 362
BanI GGYRCC 1 cut(s) 372
BanII GRGCYC 2 cut(s) 114, 1107
BauI CACGAG 1 cut(s) 808
Bbv12I GWGCWC 1 cut(s) 1224
BbvI GCAGC 7 cut(s) 231, 234, 292, 672, 901, 950, 978
BccI CCATC 8 cut(s) 317, 373, 646, 712, 717, 927, 943, 1203
BceAI ACGGC 6 cut(s) 176, 235, 352, 355, 754, 843
BciT130I CCWGG 1 cut(s) 878
BcnI CCSGG 3 cut(s) 35, 108, 268
BcoDI GTCTC 4 cut(s) 98, 344, 818, 1003
BfaI CTAG 2 cut(s) 642, 1073
BfmI CTRYAG 1 cut(s) 915
BfoI RGCGCY 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 908
BglI GCCNNNNNGGC 2 cut(s) 493, 776
BlnI CCTAGG 2 cut(s) 641, 1072
Bme1390I CCNGG 4 cut(s) 35, 108, 268, 878
Bme18I GGWCC 2 cut(s) 606, 638
BmeT110I CYCGRG 1 cut(s) 347
BmgBI CACGTC 1 cut(s) 213
BmgT120I GGNCC 5 cut(s) 172, 216, 269, 606, 638
BmiI GGNNCC 2 cut(s) 374, 607
BmrFI CCNGG 4 cut(s) 35, 108, 268, 878
BmrI ACTGGG 1 cut(s) 640
BmsI GCATC 1 cut(s) 1173
BmuI ACTGGG 1 cut(s) 640
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 3 cut(s) 35, 108, 268
BsaHI GRCGYC 1 cut(s) 1041
BsaI GGTCTC 1 cut(s) 98
BsaJI CCNNGG 9 cut(s) 107, 357, 368, 641, 774, 877, 974, 1036, 1072
BsaWI WCCGGW 1 cut(s) 1088
BsaXI ACNNNNNCTCC 2 cut(s) 842, 872
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 278, 369, 917, 1060
Bse118I RCCGGY 4 cut(s) 575, 890, 1088, 1129
Bse1I ACTGG 3 cut(s) 25, 44, 635
BseBI CCWGG 1 cut(s) 878
BseDI CCNNGG 9 cut(s) 107, 357, 368, 641, 774, 877, 974, 1036, 1072
BseGI GGATG 8 cut(s) 117, 309, 480, 723, 728, 938, 1164, 1214
BseLI CCNNNNNNNGG 5 cut(s) 257, 278, 369, 917, 1060
BseNI ACTGG 3 cut(s) 25, 44, 635
BseSI GKGCMC 1 cut(s) 1224
BseXI GCAGC 7 cut(s) 231, 234, 292, 672, 901, 950, 978
BseYI CCCAGC 2 cut(s) 204, 448
Bsh1236I CGCG 1 cut(s) 1146
BshNI GGYRCC 1 cut(s) 372
BshTI ACCGGT 1 cut(s) 1088
BsiHKAI GWGCWC 1 cut(s) 1224
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 8 cut(s) 35, 107, 267, 576, 891, 1065, 1089, 1130
BsiWI CGTACG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 5 cut(s) 257, 278, 369, 917, 1060
BsmAI GTCTC 4 cut(s) 98, 344, 818, 1003
BsmFI GGGAC 1 cut(s) 619
Bso31I GGTCTC 1 cut(s) 98
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 3 cut(s) 114, 1107, 1224
Bsp1407I TGTACA 1 cut(s) 925
Bsp143I GATC 4 cut(s) 198, 343, 745, 990
Bsp19I CCATGG 3 cut(s) 357, 774, 974
BspFNI CGCG 1 cut(s) 1146
BspLI GGNNCC 2 cut(s) 374, 607
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 1 cut(s) 740
BspQI GCTCTTC 1 cut(s) 1127
BspT107I GGYRCC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 98
BsrFI RCCGGY 4 cut(s) 575, 890, 1088, 1129
BsrGI TGTACA 1 cut(s) 925
BsrI ACTGG 3 cut(s) 25, 44, 635
BssAI RCCGGY 4 cut(s) 575, 890, 1088, 1129
BssECI CCNNGG 9 cut(s) 107, 357, 368, 641, 774, 877, 974, 1036, 1072
BssMI GATC 4 cut(s) 198, 343, 745, 990
BssNI GRCGYC 1 cut(s) 1041
BssSI CACGAG 1 cut(s) 808
BssT1I CCWWGG 5 cut(s) 357, 641, 774, 974, 1072
Bst2BI CACGAG 1 cut(s) 808
Bst2UI CCWGG 1 cut(s) 878
Bst4CI ACNGT 2 cut(s) 432, 514
Bst6I CTCTTC 1 cut(s) 1127
BstACI GRCGYC 1 cut(s) 1041
BstAUI TGTACA 1 cut(s) 925
BstC8I GCNNGC 7 cut(s) 104, 273, 303, 385, 577, 964, 971
BstDSI CCRYGG 4 cut(s) 357, 368, 774, 974
BstENI CCTNNNNNAGG 1 cut(s) 276
BstF5I GGATG 8 cut(s) 117, 309, 480, 723, 728, 938, 1164, 1214
BstFNI CGCG 1 cut(s) 1146
BstH2I RGCGCY 1 cut(s) 833
BstHHI GCGC 4 cut(s) 649, 803, 832, 1120
BstKTI GATC 4 cut(s) 201, 346, 748, 993
BstMAI GTCTC 4 cut(s) 98, 344, 818, 1003
BstMBI GATC 4 cut(s) 198, 343, 745, 990
BstMWI GCNNNNNNNGC 9 cut(s) 14, 170, 179, 188, 272, 314, 493, 534, 776
BstNI CCWGG 1 cut(s) 878
BstNSI RCATGY 3 cut(s) 106, 409, 532
BstSCI CCNGG 4 cut(s) 33, 106, 266, 876
BstSFI CTRYAG 1 cut(s) 915
BstSLI GKGCMC 1 cut(s) 1224
BstUI CGCG 1 cut(s) 1146
BstV1I GCAGC 7 cut(s) 231, 234, 292, 672, 901, 950, 978
BstX2I RGATCY 1 cut(s) 745
BstXI CCANNNNNNTGG 1 cut(s) 88
BstYI RGATCY 1 cut(s) 745
BtgI CCRYGG 4 cut(s) 357, 368, 774, 974
BtrI CACGTC 1 cut(s) 213
BtsCI GGATG 8 cut(s) 117, 309, 480, 723, 728, 938, 1164, 1214
BtsI GCAGTG 3 cut(s) 400, 621, 1065
BtsIMutI CAGTG 3 cut(s) 400, 621, 1065
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 7 cut(s) 104, 273, 303, 385, 577, 964, 971
CfoI GCGC 4 cut(s) 649, 803, 832, 1120
Cfr10I RCCGGY 4 cut(s) 575, 890, 1088, 1129
Cfr13I GGNCC 5 cut(s) 172, 216, 269, 606, 638
CseI GACGC 1 cut(s) 1030
Csp6I GTAC 6 cut(s) 10, 373, 394, 508, 583, 926
CspAI ACCGGT 1 cut(s) 1088
CviQI GTAC 6 cut(s) 10, 373, 394, 508, 583, 926
DpnI GATC 4 cut(s) 200, 345, 747, 992
DpnII GATC 4 cut(s) 198, 343, 745, 990
DraIII CACNNNGTG 2 cut(s) 369, 1060
EaeI YGGCCR 5 cut(s) 360, 414, 756, 1062, 1131
Eam1104I CTCTTC 1 cut(s) 1127
EarI CTCTTC 1 cut(s) 1127
EciI GGCGGA 3 cut(s) 39, 552, 756
Eco130I CCWWGG 5 cut(s) 357, 641, 774, 974, 1072
Eco24I GRGCYC 2 cut(s) 114, 1107
Eco31I GGTCTC 1 cut(s) 98
Eco47I GGWCC 2 cut(s) 606, 638
Eco57I CTGAAG 1 cut(s) 585
Eco88I CYCGRG 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 276
EcoO109I RGGNCCY 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 876
EcoT14I CCWWGG 5 cut(s) 357, 641, 774, 974, 1072
EcoT38I GRGCYC 2 cut(s) 114, 1107
ErhI CCWWGG 5 cut(s) 357, 641, 774, 974, 1072
FaqI GGGAC 1 cut(s) 619
FauI CCCGC 5 cut(s) 322, 572, 691, 919, 1139
FblI GTMKAC 1 cut(s) 996
FokI GGATG 8 cut(s) 104, 296, 487, 730, 735, 945, 1151, 1221
FriOI GRGCYC 2 cut(s) 114, 1107
FspBI CTAG 2 cut(s) 642, 1073
GlaI GCGC 4 cut(s) 648, 802, 831, 1119
GsaI CCCAGC 2 cut(s) 208, 452
GsuI CTGGAG 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 833
HapII CCGG 8 cut(s) 35, 107, 267, 576, 891, 1065, 1089, 1130
HgaI GACGC 1 cut(s) 1030
HhaI GCGC 4 cut(s) 649, 803, 832, 1120
Hin1I GRCGYC 1 cut(s) 1041
Hin6I GCGC 4 cut(s) 647, 801, 830, 1118
HinP1I GCGC 4 cut(s) 647, 801, 830, 1118
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HinfI GANTC 4 cut(s) 666, 811, 1011, 1068
HpaII CCGG 8 cut(s) 35, 107, 267, 576, 891, 1065, 1089, 1130
HphI GGTGA 3 cut(s) 65, 220, 973
Hpy166II GTNNAC 8 cut(s) 157, 394, 510, 825, 981, 997, 1027, 1222
Hpy188I TCNGA 2 cut(s) 869, 1208
Hpy188III TCNNGA 4 cut(s) 97, 196, 347, 349
Hpy8I GTNNAC 8 cut(s) 157, 394, 510, 825, 981, 997, 1027, 1222
Hpy99I CGWCG 4 cut(s) 197, 518, 902, 1046
HpyAV CCTTC 2 cut(s) 193, 511
HpyCH4III ACNGT 2 cut(s) 432, 514
HpyCH4IV ACGT 3 cut(s) 212, 585, 681
HpyCH4V TGCA 4 cut(s) 405, 917, 1048, 1222
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 170, 179, 188, 272, 314, 493, 534, 776
HpySE526I ACGT 3 cut(s) 212, 585, 681
Hsp92I GRCGYC 1 cut(s) 1041
HspAI GCGC 4 cut(s) 647, 801, 830, 1118
KpnI GGTACC 1 cut(s) 376
KroI GCCGGC 1 cut(s) 575
KroNI GCCGGC 1 cut(s) 577
Kzo9I GATC 4 cut(s) 198, 343, 745, 990
LguI GCTCTTC 1 cut(s) 1127
LmnI GCTCC 4 cut(s) 26, 129, 701, 869
Lsp1109I GCAGC 7 cut(s) 231, 234, 292, 672, 901, 950, 978
LweI GCATC 1 cut(s) 1173
MaeI CTAG 2 cut(s) 642, 1073
MaeII ACGT 3 cut(s) 212, 585, 681
MaeIII GTNAC 2 cut(s) 53, 208
MalI GATC 4 cut(s) 200, 345, 747, 992
MboI GATC 4 cut(s) 198, 343, 745, 990
MboII GAAGA 6 cut(s) 151, 154, 591, 835, 838, 1114
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 3 cut(s) 114, 1107, 1224
MlsI TGGCCA 1 cut(s) 362
MluNI TGGCCA 1 cut(s) 362
MlyI GAGTC 2 cut(s) 820, 1005
Mox20I TGGCCA 1 cut(s) 362
MreI CGCCGGCG 1 cut(s) 575
MroNI GCCGGC 1 cut(s) 575
MscI TGGCCA 1 cut(s) 362
MseI TTAA 1 cut(s) 1016
MslI CAYNNNNRTG 3 cut(s) 86, 986, 1169
Msp20I TGGCCA 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 611, 914
MspI CCGG 8 cut(s) 35, 107, 267, 576, 891, 1065, 1089, 1130
MspR9I CCNGG 4 cut(s) 35, 108, 268, 878
MvaI CCWGG 1 cut(s) 878
MvnI CGCG 1 cut(s) 1146
MwoI GCNNNNNNNGC 9 cut(s) 14, 170, 179, 188, 272, 314, 493, 534, 776
NaeI GCCGGC 1 cut(s) 577
NciI CCSGG 3 cut(s) 35, 108, 268
NcoI CCATGG 3 cut(s) 357, 774, 974
NdeII GATC 4 cut(s) 198, 343, 745, 990
NgoMIV GCCGGC 1 cut(s) 575
NlaIV GGNNCC 2 cut(s) 374, 607
NmeAIII GCCGAG 2 cut(s) 1017, 1126
NmuCI GTSAC 2 cut(s) 53, 208
NspI RCATGY 3 cut(s) 106, 409, 532
OliI CACNNNNGTG 1 cut(s) 86
PaeI GCATGC 1 cut(s) 106
PaeR7I CTCGAG 1 cut(s) 347
PciSI GCTCTTC 1 cut(s) 1127
PcsI WCGNNNNNNNCGW 3 cut(s) 192, 339, 345
PdiI GCCGGC 1 cut(s) 577
PfeI GAWTC 2 cut(s) 666, 1068
Pfl23II CGTACG 1 cut(s) 9
PflMI CCANNNNNTGG 2 cut(s) 369, 1060
PinAI ACCGGT 1 cut(s) 1088
PleI GAGTC 2 cut(s) 819, 1005
PpsI GAGTC 2 cut(s) 819, 1005
PpuMI RGGWCCY 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 638
Psp6I CCWGG 1 cut(s) 876
PspFI CCCAGC 2 cut(s) 204, 448
PspGI CCWGG 1 cut(s) 876
PspLI CGTACG 1 cut(s) 9
PspN4I GGNNCC 2 cut(s) 374, 607
PspPI GGNCC 5 cut(s) 172, 216, 269, 606, 638
PspPPI RGGWCCY 1 cut(s) 638
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 1 cut(s) 745
RsaI GTAC 6 cut(s) 11, 374, 395, 509, 584, 927
RsaNI GTAC 6 cut(s) 10, 373, 394, 508, 583, 926
RseI CAYNNNNRTG 3 cut(s) 86, 986, 1169
SapI GCTCTTC 1 cut(s) 1127
SaqAI TTAA 1 cut(s) 1016
Sau3AI GATC 4 cut(s) 198, 343, 745, 990
Sau96I GGNCC 5 cut(s) 172, 216, 269, 606, 638
SchI GAGTC 2 cut(s) 820, 1005
ScrFI CCNGG 4 cut(s) 35, 108, 268, 878
SduI GDGCHC 3 cut(s) 114, 1107, 1224
SfaNI GCATC 1 cut(s) 1173
SfcI CTRYAG 1 cut(s) 915
Sfr274I CTCGAG 1 cut(s) 347
SgrAI CRCCGGYG 1 cut(s) 575
SinI GGWCC 2 cut(s) 606, 638
SlaI CTCGAG 1 cut(s) 347
SmiMI CAYNNNNRTG 3 cut(s) 86, 986, 1169
SmlI CTYRAG 2 cut(s) 296, 347
SmoI CTYRAG 2 cut(s) 296, 347
SphI GCATGC 1 cut(s) 106
SspMI CTAG 2 cut(s) 642, 1073
StyD4I CCNGG 4 cut(s) 33, 106, 266, 876
StyI CCWWGG 5 cut(s) 357, 641, 774, 974, 1072
TaaI ACNGT 2 cut(s) 432, 514
TaiI ACGT 3 cut(s) 215, 588, 684
TaqI TCGA 3 cut(s) 348, 834, 1240
TatI WGTACW 2 cut(s) 393, 925
TauI GCSGC 7 cut(s) 8, 191, 416, 758, 806, 1080, 1193
TfiI GAWTC 2 cut(s) 666, 1068
Tru1I TTAA 1 cut(s) 1016
Tru9I TTAA 1 cut(s) 1016
TscAI CASTG 3 cut(s) 407, 628, 1065
TseFI GTSAC 2 cut(s) 53, 208
TseI GCWGC 7 cut(s) 244, 247, 305, 685, 914, 938, 966
Tsp45I GTSAC 2 cut(s) 53, 208
TspDTI ATGAA 6 cut(s) 157, 473, 542, 581, 607, 1189
TspGWI ACGGA 2 cut(s) 738, 850
TspRI CASTG 3 cut(s) 407, 628, 1065
Van91I CCANNNNNTGG 2 cut(s) 369, 1060
VneI GTGCAC 1 cut(s) 1220
VpaK11BI GGWCC 2 cut(s) 606, 638
XagI CCTNNNNNAGG 1 cut(s) 276
XceI RCATGY 3 cut(s) 106, 409, 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 814
XhoI CTCGAG 1 cut(s) 347
XmaJI CCTAGG 2 cut(s) 641, 1072
XmiI GTMKAC 1 cut(s) 996
XspI CTAG 2 cut(s) 642, 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.