Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0002056

Physical Location & Seq

Genomic Coordinates Reverse (-)

18271241 .. 18274641

Gene Structure 3401 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g26420.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1089 bp

ATGAGCTGCTTCAGCTGTTGTGATGAAGATGATATGCATAAAGCTACTGACAATGGAGGTCCTTACATGATGAAAAACCCTGCAGGGAATGAGGGAAGTCTTCATGCCTCTGGTAATGCGCCCAGGGGTGGTCAAGCCGTAAAGGTCCAGCCTATTGAGGTTCCGCTTATACCGGTAGAGGAGCTAAAGGAGATTACGGATAACTTCGGGACAAATGCCTTGATTGGGGAAGGTTCGTATGGAAGAGTATATTATGGGGTACTAAAAAGTGGGGAGCATGCAGCAATCAAGAAGCTAGATGCCAGCAAACAACCTGATGAGGAATATCTTGCACAGGTTTCTATGGTATCACGGCTGAAACATGATAATTTTGTTCAACTGCTTGGTTACTGTGTGGATGGAAATTCTCGTGTTCTTGCCTACGAGTTTGCATCTAATGGATCTCTCCATGATATTCTACATGGAAGGAAAGGTGTCAAAGGAGCTCAACCTGGTCCTGTTCTAACCTGGGCACAACGTGTTAAAATTGCAGTGGGTGCTGCCAGAGGACTCGAGTATTTACATGAGAAGGCGGATCCCCATATCATTCATCGCGACATCAAGTCCAGCAATGTACTAATATTTGATGATGATGTTGCTAAGATTGCAGACTTTGATTTATCCAATCAAGCTCCTGATATGGCAGCACGTCTTCACTCTACTCGTGTTCTTGGAACCTTTGGTTATCACGCCCCAGAATATGCGATGACTGGACAGTTGAATGCCAAAAGTGATGTTTACAGTTTTGGTGTTGTGCTGCTAGAGCTCTTGACTGGAAGAAAACCTGTTGATCATACATTACCACGGGGACAGCAGAGCCTAGTGACATGGGCTACACCAAAACTCAGTGAAGACAAAGTTAGGCAGTGTGTTGATGCAAGACTAGGAGGAGAATTCCCCGCAAAGGCTGTTGCGAAGATGGCGGCTGTTGCTGCCTTGTGTGTGCAATATGAAGCTGATTTCCGCCCAAACATGAGCATTGTGGTCAAAGCCCTACAACCCCTGCTAAATGCTCGGCCTGGACCCACTGGTGAATCTCCAAGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

363

Amino Acids

39.15

Weight (kDa)

6.31

Isoelectric Point (pI)

28.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	72 - 279	5.5e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	73 - 345	1.5e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002056)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47060 AT2G47060 AT2G47060 AT2G47060 AT3G62220 AT3G62220
fragaria_vesca	FvH4_1g26420 FvH4_1g26420
malus_domestica	MD09G1292500.v1.1 MD17G1283600.v1.1
prunus_persica	Prupe.3G003900_v2.0.a1 Prupe.3G003900_v2.0.a1 Prupe.3G003900_v2.0.a1
pyrus_communis	pycom09g19700
rosa_chinensis	RchiOBHm_Chr3g0486191 RchiOBHm_Chr5g0052601
rosa_laevigata	RLG00000023094 RLG00000034754
rosa_multiflora	Rmu_co8331037.1_g000001 Rmu_sc0001209.1_g000013 Rmu_sc0001851.1_g000047 Rmu_sc0003459.1_g000004 Rmu_sc0017021.1_g000001
rosa_roxburghii	Rroxscaffold_1G00008910 Rroxscaffold_6G00394750 Rroxscaffold_6G00396000
rosa_rugosa	Rorug02G0403900 Rorug02G0403900 Rorug02G0403900 Rorug03G0223900 Rorug03G0223900 Rorug03G0224000 Rorug06G0040100
rosa_samantha	Rh1AG084600 Rh1CG082400 Rh1DG088900 Rh3AG273900 Rh3AG282400 Rh3BG309200 Rh3DG303800 Rh3DG314200 Rh5BG354300 Rh5DG369900
rosa_wichuraiana	Rw3G024270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	594
AciI	CCGC	5 cut(s)	164, 572, 939, 962, 1003
AclWI	GGATC	3 cut(s)	448, 569, 582
AcsI	RAATTY	2 cut(s)	403, 932
AdeI	CACNNNGTG	1 cut(s)	518
AfaI	GTAC	2 cut(s)	261, 615
AfiI	CCNNNNNNNGG	3 cut(s)	128, 225, 943
AflIII	ACRYGT	1 cut(s)	517
AgeI	ACCGGT	1 cut(s)	172
AgsI	TTSAA	2 cut(s)	377, 760
AhdI	GACNNNNNGTC	1 cut(s)	601
AjiI	CACGTC	1 cut(s)	689
AjnI	CCWGG	4 cut(s)	122, 490, 506, 1057
Alw21I	GWGCWC	2 cut(s)	487, 807
AlwI	GGATC	3 cut(s)	448, 569, 582
Ama87I	CYCGRG	1 cut(s)	551
AoxI	GGCC	1 cut(s)	1055
ApeKI	GCWGC	6 cut(s)	6, 281, 539, 683, 796, 971
ApoI	RAATTY	2 cut(s)	403, 932
AsiGI	ACCGGT	1 cut(s)	172
AspLEI	GCGC	1 cut(s)	121
AspS9I	GGNCC	4 cut(s)	59, 145, 494, 1061
AsuHPI	GGTGA	1 cut(s)	1082
AvaI	CYCGRG	1 cut(s)	551
AvaII	GGWCC	4 cut(s)	59, 145, 494, 1061
BaeGI	GKGCMC	1 cut(s)	514
BamHI	GGATCC	1 cut(s)	574
BanII	GRGCYC	2 cut(s)	487, 807
BauI	CACGAG	2 cut(s)	408, 702
BbsI	GAAGAC	3 cut(s)	92, 683, 897
Bbv12I	GWGCWC	2 cut(s)	487, 807
BbvI	GCAGC	5 cut(s)	293, 526, 695, 783, 958
BccI	CCATC	2 cut(s)	392, 952
BceAI	ACGGC	2 cut(s)	122, 368
BciT130I	CCWGG	4 cut(s)	124, 492, 508, 1059
BclI	TGATCA	1 cut(s)	829
BfaI	CTAG	4 cut(s)	296, 800, 860, 923
BfmI	CTRYAG	1 cut(s)	81
BisI	GCNGC	7 cut(s)	7, 282, 540, 684, 797, 963, 972
BlsI	GCNGC	7 cut(s)	8, 283, 541, 685, 798, 964, 973
Bme1390I	CCNGG	4 cut(s)	124, 492, 508, 1059
Bme18I	GGWCC	4 cut(s)	59, 145, 494, 1061
BmeRI	GACNNNNNGTC	1 cut(s)	601
BmeT110I	CYCGRG	1 cut(s)	551
BmgBI	CACGTC	1 cut(s)	689
BmgT120I	GGNCC	4 cut(s)	59, 145, 494, 1061
BmiI	GGNNCC	4 cut(s)	162, 576, 715, 1063
BmrFI	CCNGG	4 cut(s)	124, 492, 508, 1059
BmsI	GCATC	3 cut(s)	289, 440, 904
BpiI	GAAGAC	3 cut(s)	92, 683, 897
BsaJI	CCNNGG	4 cut(s)	122, 123, 507, 842
BsaWI	WCCGGW	1 cut(s)	172
Bsc4I	CCNNNNNNNGG	3 cut(s)	128, 225, 943
Bse118I	RCCGGY	1 cut(s)	172
Bse1I	ACTGG	3 cut(s)	754, 817, 1072
Bse3DI	GCAATG	1 cut(s)	616
BseBI	CCWGG	4 cut(s)	124, 492, 508, 1059
BseDI	CCNNGG	4 cut(s)	122, 123, 507, 842
BseGI	GGATG	1 cut(s)	403
BseLI	CCNNNNNNNGG	3 cut(s)	128, 225, 943
BseMI	GCAATG	1 cut(s)	616
BseMII	CTCAG	1 cut(s)	898
BseNI	ACTGG	3 cut(s)	754, 817, 1072
BseRI	GAGGAG	2 cut(s)	194, 942
BseSI	GKGCMC	1 cut(s)	514
BseXI	GCAGC	5 cut(s)	293, 526, 695, 783, 958
Bsh1236I	CGCG	1 cut(s)	594
BshFI	GGCC	1 cut(s)	1057
BshTI	ACCGGT	1 cut(s)	172
BsiHKAI	GWGCWC	2 cut(s)	487, 807
BsiHKCI	CYCGRG	1 cut(s)	551
BsiSI	CCGG	1 cut(s)	173
BslFI	GGGAC	2 cut(s)	223, 861
BslI	CCNNNNNNNGG	3 cut(s)	128, 225, 943
BsmFI	GGGAC	2 cut(s)	223, 861
BsmI	GAATGC	1 cut(s)	766
BsnI	GGCC	1 cut(s)	1057
BsoBI	CYCGRG	1 cut(s)	551
Bsp1286I	GDGCHC	3 cut(s)	487, 514, 807
Bsp143I	GATC	3 cut(s)	440, 574, 829
Bsp68I	TCGCGA	1 cut(s)	594
BspACI	CCGC	5 cut(s)	164, 572, 939, 962, 1003
BspANI	GGCC	1 cut(s)	1057
BspCNI	CTCAG	1 cut(s)	897
BspFNI	CGCG	1 cut(s)	594
BspLI	GGNNCC	4 cut(s)	162, 576, 715, 1063
BspMAI	CTGCAG	1 cut(s)	85
BspPI	GGATC	3 cut(s)	448, 569, 582
BsrDI	GCAATG	1 cut(s)	616
BsrFI	RCCGGY	1 cut(s)	172
BsrI	ACTGG	3 cut(s)	754, 817, 1072
BssAI	RCCGGY	1 cut(s)	172
BssECI	CCNNGG	4 cut(s)	122, 123, 507, 842
BssMI	GATC	3 cut(s)	440, 574, 829
BssSI	CACGAG	2 cut(s)	408, 702
Bst2BI	CACGAG	2 cut(s)	408, 702
Bst2UI	CCWGG	4 cut(s)	124, 492, 508, 1059
Bst4CI	ACNGT	3 cut(s)	392, 756, 782
Bst6I	CTCTTC	1 cut(s)	238
BstAPI	GCANNNNNTGC	1 cut(s)	536
BstC8I	GCNNGC	2 cut(s)	279, 304
BstDEI	CTNAG	2 cut(s)	639, 884
BstDSI	CCRYGG	1 cut(s)	842
BstF5I	GGATG	1 cut(s)	403
BstFNI	CGCG	1 cut(s)	594
BstHHI	GCGC	1 cut(s)	121
BstKTI	GATC	3 cut(s)	443, 577, 832
BstMBI	GATC	3 cut(s)	440, 574, 829
BstMWI	GCNNNNNNNGC	7 cut(s)	12, 536, 644, 802, 959, 968, 971
BstNI	CCWGG	4 cut(s)	124, 492, 508, 1059
BstNSI	RCATGY	1 cut(s)	281
BstSCI	CCNGG	4 cut(s)	122, 490, 506, 1057
BstSFI	CTRYAG	1 cut(s)	81
BstSLI	GKGCMC	1 cut(s)	514
BstUI	CGCG	1 cut(s)	594
BstV1I	GCAGC	5 cut(s)	293, 526, 695, 783, 958
BstV2I	GAAGAC	3 cut(s)	92, 683, 897
BstX2I	RGATCY	2 cut(s)	440, 574
BstYI	RGATCY	2 cut(s)	440, 574
BsuRI	GGCC	1 cut(s)	1057
BtgI	CCRYGG	1 cut(s)	842
BtgZI	GCGATG	2 cut(s)	575, 758
BtrI	CACGTC	1 cut(s)	689
BtsCI	GGATG	1 cut(s)	403
BtsI	GCAGTG	2 cut(s)	537, 911
BtsIMutI	CAGTG	4 cut(s)	537, 892, 911, 1065
BtuMI	TCGCGA	1 cut(s)	594
Cac8I	GCNNGC	2 cut(s)	279, 304
CfoI	GCGC	1 cut(s)	121
Cfr10I	RCCGGY	1 cut(s)	172
Cfr13I	GGNCC	4 cut(s)	59, 145, 494, 1061
CsiI	ACCWGGT	1 cut(s)	490
Csp6I	GTAC	2 cut(s)	260, 614
CspAI	ACCGGT	1 cut(s)	172
CviAII	CATG	9 cut(s)	67, 104, 278, 362, 449, 461, 563, 867, 1012
CviQI	GTAC	2 cut(s)	260, 614
DdeI	CTNAG	2 cut(s)	639, 884
DpnI	GATC	3 cut(s)	442, 576, 831
DpnII	GATC	3 cut(s)	440, 574, 829
DraIII	CACNNNGTG	1 cut(s)	518
DriI	GACNNNNNGTC	1 cut(s)	601
Eam1104I	CTCTTC	1 cut(s)	238
Eam1105I	GACNNNNNGTC	1 cut(s)	601
EarI	CTCTTC	1 cut(s)	238
EciI	GGCGGA	2 cut(s)	587, 992
Ecl136II	GAGCTC	2 cut(s)	485, 805
Eco24I	GRGCYC	2 cut(s)	487, 807
Eco47I	GGWCC	4 cut(s)	59, 145, 494, 1061
Eco53kI	GAGCTC	2 cut(s)	485, 805
Eco88I	CYCGRG	1 cut(s)	551
EcoICRI	GAGCTC	2 cut(s)	485, 805
EcoO109I	RGGNCCY	1 cut(s)	59
EcoRI	GAATTC	1 cut(s)	932
EcoRII	CCWGG	4 cut(s)	122, 490, 506, 1057
EcoT22I	ATGCAT	1 cut(s)	39
EcoT38I	GRGCYC	2 cut(s)	487, 807
FaeI	CATG	9 cut(s)	70, 107, 281, 365, 452, 464, 566, 870, 1015
FaqI	GGGAC	2 cut(s)	223, 861
FatI	CATG	9 cut(s)	66, 103, 277, 361, 448, 460, 562, 866, 1011
FauI	CCCGC	1 cut(s)	946
FbaI	TGATCA	1 cut(s)	829
Fnu4HI	GCNGC	7 cut(s)	7, 282, 540, 684, 797, 963, 972
FokI	GGATG	1 cut(s)	410
FriOI	GRGCYC	2 cut(s)	487, 807
Fsp4HI	GCNGC	7 cut(s)	7, 282, 540, 684, 797, 963, 972
FspBI	CTAG	4 cut(s)	296, 800, 860, 923
GlaI	GCGC	1 cut(s)	120
GluI	GCNGC	7 cut(s)	7, 282, 540, 684, 797, 963, 972
HaeIII	GGCC	1 cut(s)	1057
HapII	CCGG	1 cut(s)	173
HhaI	GCGC	1 cut(s)	121
Hin1II	CATG	9 cut(s)	70, 107, 281, 365, 452, 464, 566, 870, 1015
Hin6I	GCGC	1 cut(s)	119
HinP1I	GCGC	1 cut(s)	119
HindIII	AAGCTT	1 cut(s)	1081
HinfI	GANTC	2 cut(s)	549, 1073
HpaII	CCGG	1 cut(s)	173
HphI	GGTGA	1 cut(s)	1082
Hpy166II	GTNNAC	1 cut(s)	778
Hpy188III	TCNNGA	5 cut(s)	208, 289, 593, 674, 808
Hpy8I	GTNNAC	1 cut(s)	778
HpyAV	CCTTC	3 cut(s)	224, 459, 562
HpyCH4III	ACNGT	3 cut(s)	392, 756, 782
HpyCH4IV	ACGT	2 cut(s)	517, 688
HpyCH4V	TGCA	9 cut(s)	37, 83, 281, 332, 431, 530, 647, 917, 985
HpyF10VI	GCNNNNNNNGC	7 cut(s)	12, 536, 644, 802, 959, 968, 971
HpyF3I	CTNAG	2 cut(s)	639, 884
HpySE526I	ACGT	2 cut(s)	517, 688
Hsp92II	CATG	9 cut(s)	70, 107, 281, 365, 452, 464, 566, 870, 1015
HspAI	GCGC	1 cut(s)	119
Ksp22I	TGATCA	1 cut(s)	829
Kzo9I	GATC	3 cut(s)	440, 574, 829
LmnI	GCTCC	4 cut(s)	181, 274, 482, 676
Lsp1109I	GCAGC	5 cut(s)	293, 526, 695, 783, 958
LweI	GCATC	3 cut(s)	289, 440, 904
MabI	ACCWGGT	1 cut(s)	490
MaeI	CTAG	4 cut(s)	296, 800, 860, 923
MaeII	ACGT	2 cut(s)	517, 688
MaeIII	GTNAC	2 cut(s)	386, 862
MalI	GATC	3 cut(s)	442, 576, 831
MboI	GATC	3 cut(s)	440, 574, 829
MboII	GAAGA	7 cut(s)	38, 92, 255, 683, 828, 902, 967
MflI	RGATCY	2 cut(s)	440, 574
MhlI	GDGCHC	3 cut(s)	487, 514, 807
MluCI	AATT	4 cut(s)	367, 403, 525, 932
MlyI	GAGTC	1 cut(s)	543
MnlI	CCTC	8 cut(s)	50, 85, 118, 151, 172, 313, 539, 920
Mph1103I	ATGCAT	1 cut(s)	39
MseI	TTAA	1 cut(s)	522
MspA1I	CMGCKG	1 cut(s)	15
MspI	CCGG	1 cut(s)	173
MspR9I	CCNGG	4 cut(s)	124, 492, 508, 1059
Mva1269I	GAATGC	1 cut(s)	766
MvaI	CCWGG	4 cut(s)	124, 492, 508, 1059
MvnI	CGCG	1 cut(s)	594
MwoI	GCNNNNNNNGC	7 cut(s)	12, 536, 644, 802, 959, 968, 971
NdeII	GATC	3 cut(s)	440, 574, 829
NlaIII	CATG	9 cut(s)	70, 107, 281, 365, 452, 464, 566, 870, 1015
NlaIV	GGNNCC	4 cut(s)	162, 576, 715, 1063
NmeAIII	GCCGAG	1 cut(s)	1033
NmuCI	GTSAC	1 cut(s)	862
NruI	TCGCGA	1 cut(s)	594
NsiI	ATGCAT	1 cut(s)	39
NspI	RCATGY	1 cut(s)	281
PaeI	GCATGC	1 cut(s)	281
PaeR7I	CTCGAG	1 cut(s)	551
PasI	CCCWGGG	1 cut(s)	123
PctI	GAATGC	1 cut(s)	766
PfeI	GAWTC	1 cut(s)	1073
PinAI	ACCGGT	1 cut(s)	172
PkrI	GCNGC	7 cut(s)	8, 283, 541, 685, 798, 964, 973
PleI	GAGTC	1 cut(s)	543
PpsI	GAGTC	1 cut(s)	543
PpuMI	RGGWCCY	1 cut(s)	59
Psp124BI	GAGCTC	2 cut(s)	487, 807
Psp5II	RGGWCCY	1 cut(s)	59
Psp6I	CCWGG	4 cut(s)	122, 490, 506, 1057
PspGI	CCWGG	4 cut(s)	122, 490, 506, 1057
PspN4I	GGNNCC	4 cut(s)	162, 576, 715, 1063
PspPI	GGNCC	4 cut(s)	59, 145, 494, 1061
PspPPI	RGGWCCY	1 cut(s)	59
PspXI	VCTCGAGB	1 cut(s)	551
PstI	CTGCAG	1 cut(s)	85
PsuI	RGATCY	2 cut(s)	440, 574
PvuII	CAGCTG	1 cut(s)	15
RruI	TCGCGA	1 cut(s)	594
RsaI	GTAC	2 cut(s)	261, 615
RsaNI	GTAC	2 cut(s)	260, 614
SacI	GAGCTC	2 cut(s)	487, 807
SaqAI	TTAA	1 cut(s)	522
SatI	GCNGC	7 cut(s)	7, 282, 540, 684, 797, 963, 972
Sau3AI	GATC	3 cut(s)	440, 574, 829
Sau96I	GGNCC	4 cut(s)	59, 145, 494, 1061
SbfI	CCTGCAGG	1 cut(s)	85
SchI	GAGTC	1 cut(s)	543
ScrFI	CCNGG	4 cut(s)	124, 492, 508, 1059
SdaI	CCTGCAGG	1 cut(s)	85
SduI	GDGCHC	3 cut(s)	487, 514, 807
SexAI	ACCWGGT	1 cut(s)	490
SfaNI	GCATC	3 cut(s)	289, 440, 904
SfcI	CTRYAG	1 cut(s)	81
Sfr274I	CTCGAG	1 cut(s)	551
SinI	GGWCC	4 cut(s)	59, 145, 494, 1061
SlaI	CTCGAG	1 cut(s)	551
SmlI	CTYRAG	1 cut(s)	551
SmoI	CTYRAG	1 cut(s)	551
SphI	GCATGC	1 cut(s)	281
Sse8387I	CCTGCAGG	1 cut(s)	85
Sse9I	AATT	4 cut(s)	367, 403, 525, 932
SsiI	CCGC	5 cut(s)	164, 572, 939, 962, 1003
SspI	AATATT	1 cut(s)	621
SspMI	CTAG	4 cut(s)	296, 800, 860, 923
SstI	GAGCTC	2 cut(s)	487, 807
StyD4I	CCNGG	4 cut(s)	122, 490, 506, 1057
TaaI	ACNGT	3 cut(s)	392, 756, 782
TaiI	ACGT	2 cut(s)	520, 691
TaqI	TCGA	1 cut(s)	552
TasI	AATT	4 cut(s)	367, 403, 525, 932
TatI	WGTACW	1 cut(s)	613
TauI	GCSGC	1 cut(s)	965
TfiI	GAWTC	1 cut(s)	1073
Tru1I	TTAA	1 cut(s)	522
Tru9I	TTAA	1 cut(s)	522
TscAI	CASTG	4 cut(s)	537, 892, 911, 1072
TseFI	GTSAC	1 cut(s)	862
TseI	GCWGC	6 cut(s)	6, 281, 539, 683, 796, 971
Tsp45I	GTSAC	1 cut(s)	862
TspDTI	ATGAA	5 cut(s)	39, 86, 92, 578, 1005
TspGWI	ACGGA	1 cut(s)	212
TspRI	CASTG	4 cut(s)	537, 892, 911, 1072
VpaK11BI	GGWCC	4 cut(s)	59, 145, 494, 1061
XapI	RAATTY	2 cut(s)	403, 932
XceI	RCATGY	1 cut(s)	281
XhoI	CTCGAG	1 cut(s)	551
XspI	CTAG	4 cut(s)	296, 800, 860, 923
Zsp2I	ATGCAT	1 cut(s)	39

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.