FvH4_2g02870

branched-chain-amino-acid transaminase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002089
Physical Location & Seq
Reverse (-)
2254448 .. 2258069
3622 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02870.t2

Sequence Viewer

Length: 1080 bp
ATGCCTTCTTCACCTTTGCAAACAACCACTGAATCTCACAACCACAGCCTGAAAAACGGTGAACGGTATGCTGAAGGCATTAACTGGGATGATCTTGGGTTTGGACTGATTCCAATCGATTACATGTACACTATGAAATGCTCCGACAGAGAAAACTTTACACAAGGACAACTCTCTCCCTTTGGAAAGATTGAGCTCTGTCCATCTGCTGGAATATTAAATTATGCACAGGGACTATACGAAGGTCTTAAGGCAATTAGAACAGAAGATGGTCGTATTTTGTTGTTTCGGACTAAAGAGAATGCTCTGAGAATGAAGATGGGTGCAGATAGATTGTGCATGCCATCTCCATCTGTTGAGCAATTTGTTGAGGCTGTGATGCAAACTGTCCAAGCCAACAAGCGTTGGGTACCACCTCCAGGAAAAGGATCACTGTATGTTAGGCCTTTGCTCATGGCGAGTGGATGTAATCTGGGTTTGGGGCCGGGAAAGGAGTATACATTCGTAATCTATGCTTCTCCAGTTGGTAATTATATCAAGGGAAGGGCGGCCCTCAATTTATTTGTTGAACATGTTTACCACAGAGCTGCTCCTGGTGGAACTGGAGGTGTCAAGTCTGTCACCAATTACTCACCTGTTTATCAGCCACTAACGCAAGCAAGGGCCAAGGGGTTCTCTGATATCTTATTCCTAGATTCGGAAACTGGTAAAAACATTGAGGAGATTGCAGCAGCTAACATTTTCATTCTCAAGGGTAATGTTCTATCAACTCCAACATTGAAACTAGGAACTATCCTCCCTGGAATAACCCGTAAAAGCATCATTGACATTGCCCGTGATTTTGGTTACCAGGTGGAGGAGAGTGTGATCCCAGTGGAGGATTTACTTGAAGCTGACGAAGCTTTCTGCACTGGAACTGCCGTGATTGTCAACTCAGTTGGTTCAGTCACTTATCAAGACAAGAGGGTTGAATATAAAACAGGAGAAGGATCATTATGTCAGAAGCTGTATGAAACACTAACAGGAATCCAAACAGGTCGTCTTGAGGACAAGAATGGATGGACGCTAGAGGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

360

Amino Acids

39.38

Weight (kDa)

5.93

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 88 - 321 3.9e-31 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 209
AccI GTMKAC 1 cut(s) 497
AciI CCGC 1 cut(s) 548
AclWI GGATC 3 cut(s) 436, 862, 997
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 2 cut(s) 128, 411
AfiI CCNNNNNNNGG 6 cut(s) 209, 419, 425, 697, 856, 877
AflII CTTAAG 1 cut(s) 248
AflIII ACRYGT 2 cut(s) 123, 571
AgsI TTSAA 4 cut(s) 569, 781, 890, 971
AjnI CCWGG 4 cut(s) 418, 592, 799, 849
AluBI AGCT 6 cut(s) 196, 587, 734, 893, 902, 1006
AluI AGCT 6 cut(s) 196, 587, 734, 893, 902, 1006
Alw21I GWGCWC 1 cut(s) 198
AlwI GGATC 3 cut(s) 436, 862, 997
AlwNI CAGNNNCTG 1 cut(s) 1006
AoxI GGCC 4 cut(s) 443, 482, 549, 663
ApeKI GCWGC 3 cut(s) 587, 728, 731
ArsI GACNNNNNNTTYG 2 cut(s) 1024, 1056
Asp718I GGTACC 1 cut(s) 409
AspS9I GGNCC 3 cut(s) 482, 550, 663
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 4 cut(s) 3, 71, 613, 624
BanI GGYRCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 3 cut(s) 574, 740, 743
BccI CCATC 6 cut(s) 211, 263, 313, 352, 358, 1053
BceAI ACGGC 1 cut(s) 905
BciT130I CCWGG 4 cut(s) 420, 594, 801, 851
BcnI CCSGG 1 cut(s) 486
BfaI CTAG 3 cut(s) 692, 785, 1067
BfrI CTTAAG 1 cut(s) 248
BisI GCNGC 4 cut(s) 549, 588, 729, 732
BlsI GCNGC 4 cut(s) 550, 589, 730, 733
Bme1390I CCNGG 5 cut(s) 420, 486, 594, 801, 851
BmgT120I GGNCC 3 cut(s) 482, 550, 663
BmiI GGNNCC 2 cut(s) 411, 483
BmrFI CCNGG 5 cut(s) 420, 486, 594, 801, 851
BmrI ACTGGG 2 cut(s) 94, 866
BmsI GCATC 2 cut(s) 369, 828
BmuI ACTGGG 2 cut(s) 94, 866
BpmI CTGGAG 3 cut(s) 402, 504, 624
BpuEI CTTGAG 2 cut(s) 734, 1064
BpuMI CCSGG 1 cut(s) 486
Bsa29I ATCGAT 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 2 cut(s) 666, 799
Bsc4I CCNNNNNNNGG 6 cut(s) 209, 419, 425, 697, 856, 877
Bse1I ACTGG 6 cut(s) 89, 521, 607, 709, 872, 916
Bse3DI GCAATG 1 cut(s) 828
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 4 cut(s) 420, 594, 801, 851
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 666, 799
BseGI GGATG 3 cut(s) 94, 470, 1064
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 6 cut(s) 209, 419, 425, 697, 856, 877
BseMI GCAATG 1 cut(s) 828
BseMII CTCAG 2 cut(s) 299, 948
BseNI ACTGG 6 cut(s) 89, 521, 607, 709, 872, 916
BseRI GAGGAG 2 cut(s) 734, 872
BseXI GCAGC 3 cut(s) 574, 740, 743
BsgI GTGCAG 2 cut(s) 345, 892
BshFI GGCC 4 cut(s) 445, 484, 551, 665
BshNI GGYRCC 1 cut(s) 409
BshVI ATCGAT 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 1 cut(s) 485
BslFI GGGAC 1 cut(s) 246
BslI CCNNNNNNNGG 6 cut(s) 209, 419, 425, 697, 856, 877
BsmFI GGGAC 1 cut(s) 246
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 4 cut(s) 445, 484, 551, 665
Bsp1286I GDGCHC 1 cut(s) 198
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 4 cut(s) 91, 428, 867, 989
BspACI CCGC 1 cut(s) 548
BspANI GGCC 4 cut(s) 445, 484, 551, 665
BspCNI CTCAG 2 cut(s) 300, 947
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 411, 483
BspPI GGATC 3 cut(s) 436, 862, 997
BspT107I GGYRCC 1 cut(s) 409
BspTI CTTAAG 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 828
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 6 cut(s) 89, 521, 607, 709, 872, 916
BssECI CCNNGG 2 cut(s) 666, 799
BssMI GATC 4 cut(s) 91, 428, 867, 989
BssNAI GTATAC 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 666
Bst1107I GTATAC 1 cut(s) 498
Bst2UI CCWGG 4 cut(s) 420, 594, 801, 851
Bst4CI ACNGT 4 cut(s) 59, 66, 388, 435
BstAFI CTTAAG 1 cut(s) 248
BstAUI TGTACA 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 341, 657
BstDEI CTNAG 2 cut(s) 308, 934
BstEII GGTNACC 1 cut(s) 845
BstF5I GGATG 3 cut(s) 94, 470, 1064
BstKTI GATC 4 cut(s) 94, 431, 870, 992
BstMBI GATC 4 cut(s) 91, 428, 867, 989
BstMWI GCNNNNNNNGC 2 cut(s) 652, 899
BstNI CCWGG 4 cut(s) 420, 594, 801, 851
BstNSI RCATGY 3 cut(s) 127, 343, 575
BstPI GGTNACC 1 cut(s) 845
BstSCI CCNGG 5 cut(s) 418, 484, 592, 799, 849
BstV1I GCAGC 3 cut(s) 574, 740, 743
BstZ17I GTATAC 1 cut(s) 498
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 4 cut(s) 445, 484, 551, 665
BsuTUI ATCGAT 1 cut(s) 117
BtsCI GGATG 3 cut(s) 94, 470, 1064
BtsIMutI CAGTG 4 cut(s) 27, 431, 879, 909
Cac8I GCNNGC 2 cut(s) 341, 657
CaiI CAGNNNCTG 1 cut(s) 1006
Cfr13I GGNCC 3 cut(s) 482, 550, 663
ClaI ATCGAT 1 cut(s) 117
CseI GACGC 1 cut(s) 1072
CsiI ACCWGGT 1 cut(s) 849
Csp6I GTAC 2 cut(s) 127, 410
CviAII CATG 4 cut(s) 124, 340, 454, 572
CviQI GTAC 2 cut(s) 127, 410
DdeI CTNAG 2 cut(s) 308, 934
DpnI GATC 4 cut(s) 93, 430, 869, 991
DpnII GATC 4 cut(s) 91, 428, 867, 989
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 666
Eco147I AGGCCT 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 682
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 93
Eco91I GGTNACC 1 cut(s) 845
EcoICRI GAGCTC 1 cut(s) 196
EcoO65I GGTNACC 1 cut(s) 845
EcoRII CCWGG 4 cut(s) 418, 592, 799, 849
EcoRV GATATC 1 cut(s) 682
EcoT14I CCWWGG 1 cut(s) 666
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 666
FaeI CATG 4 cut(s) 127, 343, 457, 575
FaqI GGGAC 1 cut(s) 246
FatI CATG 4 cut(s) 123, 339, 453, 571
FblI GTMKAC 1 cut(s) 497
Fnu4HI GCNGC 4 cut(s) 549, 588, 729, 732
FokI GGATG 3 cut(s) 101, 477, 1071
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 4 cut(s) 549, 588, 729, 732
FspBI CTAG 3 cut(s) 692, 785, 1067
GluI GCNGC 4 cut(s) 549, 588, 729, 732
GsuI CTGGAG 3 cut(s) 402, 504, 624
HaeIII GGCC 4 cut(s) 445, 484, 551, 665
HapII CCGG 1 cut(s) 485
HgaI GACGC 1 cut(s) 1072
Hin1II CATG 4 cut(s) 127, 343, 457, 575
HincII GTYRAC 2 cut(s) 931, 1075
HindII GTYRAC 2 cut(s) 931, 1075
HindIII AAGCTT 1 cut(s) 900
HinfI GANTC 4 cut(s) 32, 109, 695, 1026
HpaII CCGG 1 cut(s) 485
HphI GGTGA 4 cut(s) 3, 71, 613, 624
Hpy166II GTNNAC 6 cut(s) 62, 129, 498, 577, 931, 1075
Hpy188I TCNGA 6 cut(s) 145, 291, 309, 679, 700, 1002
Hpy188III TCNNGA 2 cut(s) 956, 1043
Hpy8I GTNNAC 6 cut(s) 62, 129, 498, 577, 931, 1075
HpyAV CCTTC 5 cut(s) 15, 68, 236, 537, 980
HpyCH4III ACNGT 4 cut(s) 59, 66, 388, 435
HpyCH4V TGCA 7 cut(s) 19, 227, 326, 339, 382, 728, 909
HpyF10VI GCNNNNNNNGC 2 cut(s) 652, 899
HpyF3I CTNAG 2 cut(s) 308, 934
Hsp92II CATG 4 cut(s) 127, 343, 457, 575
KpnI GGTACC 1 cut(s) 413
Kzo9I GATC 4 cut(s) 91, 428, 867, 989
LmnI GCTCC 2 cut(s) 146, 595
Lsp1109I GCAGC 3 cut(s) 574, 740, 743
LweI GCATC 2 cut(s) 369, 828
MabI ACCWGGT 1 cut(s) 849
MaeI CTAG 3 cut(s) 692, 785, 1067
MaeIII GTNAC 3 cut(s) 619, 845, 946
MalI GATC 4 cut(s) 93, 430, 869, 991
MboI GATC 4 cut(s) 91, 428, 867, 989
MboII GAAGA 2 cut(s) 278, 328
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 6 cut(s) 220, 255, 362, 529, 556, 625
MmeI TCCRAC 2 cut(s) 168, 797
MseI TTAA 3 cut(s) 81, 218, 249
MspCI CTTAAG 1 cut(s) 248
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 5 cut(s) 420, 486, 594, 801, 851
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 4 cut(s) 420, 594, 801, 851
MwoI GCNNNNNNNGC 2 cut(s) 652, 899
NciI CCSGG 1 cut(s) 486
NdeII GATC 4 cut(s) 91, 428, 867, 989
NlaIII CATG 4 cut(s) 127, 343, 457, 575
NlaIV GGNNCC 2 cut(s) 411, 483
NmuCI GTSAC 2 cut(s) 619, 946
NspI RCATGY 3 cut(s) 127, 343, 575
PaeI GCATGC 1 cut(s) 343
PceI AGGCCT 1 cut(s) 445
PciI ACATGT 2 cut(s) 123, 571
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 4 cut(s) 32, 109, 695, 1026
PflMI CCANNNNNTGG 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 4 cut(s) 550, 589, 730, 733
PscI ACATGT 2 cut(s) 123, 571
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 4 cut(s) 418, 592, 799, 849
PspEI GGTNACC 1 cut(s) 845
PspGI CCWGG 4 cut(s) 418, 592, 799, 849
PspN4I GGNNCC 2 cut(s) 411, 483
PspPI GGNCC 3 cut(s) 482, 550, 663
PstNI CAGNNNCTG 1 cut(s) 1006
RsaI GTAC 2 cut(s) 128, 411
RsaNI GTAC 2 cut(s) 127, 410
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 3 cut(s) 81, 218, 249
SatI GCNGC 4 cut(s) 549, 588, 729, 732
Sau3AI GATC 4 cut(s) 91, 428, 867, 989
Sau96I GGNCC 3 cut(s) 482, 550, 663
ScrFI CCNGG 5 cut(s) 420, 486, 594, 801, 851
SduI GDGCHC 1 cut(s) 198
SexAI ACCWGGT 1 cut(s) 849
SfaNI GCATC 2 cut(s) 369, 828
SmlI CTYRAG 3 cut(s) 248, 749, 1043
SmoI CTYRAG 3 cut(s) 248, 749, 1043
SphI GCATGC 1 cut(s) 343
Sse9I AATT 6 cut(s) 220, 255, 362, 529, 556, 625
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 1 cut(s) 548
SspI AATATT 1 cut(s) 216
SspMI CTAG 3 cut(s) 692, 785, 1067
SstI GAGCTC 1 cut(s) 198
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 5 cut(s) 418, 484, 592, 799, 849
StyI CCWWGG 1 cut(s) 666
TaaI ACNGT 4 cut(s) 59, 66, 388, 435
TaqI TCGA 1 cut(s) 117
TasI AATT 6 cut(s) 220, 255, 362, 529, 556, 625
TatI WGTACW 1 cut(s) 126
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 4 cut(s) 32, 109, 695, 1026
Tru1I TTAA 3 cut(s) 81, 218, 249
Tru9I TTAA 3 cut(s) 81, 218, 249
TscAI CASTG 4 cut(s) 34, 438, 879, 916
TseFI GTSAC 2 cut(s) 619, 946
TseI GCWGC 3 cut(s) 587, 728, 731
Tsp45I GTSAC 2 cut(s) 619, 946
TspDTI ATGAA 4 cut(s) 149, 329, 733, 1026
TspRI CASTG 4 cut(s) 34, 438, 879, 916
Van91I CCANNNNNTGG 1 cut(s) 209
Vha464I CTTAAG 1 cut(s) 248
XceI RCATGY 3 cut(s) 127, 343, 575
XmiI GTMKAC 1 cut(s) 497
XspI CTAG 3 cut(s) 692, 785, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.