MD05G1110500.v1.1

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002089
Physical Location & Seq
Forward (+)
22742992 .. 22749005
6014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1110500.v1.1.491

Sequence Viewer

Length: 852 bp
ATGTTTGGAATATTAGAAGGTCTCAAGGCAATCAGGACGAAAGATGGTCGCATTCAGCTGTTTCGCCCGGAAGAGAATGCTCTAAGAATGAAGACGGGTGCAGAGAGATTGTGCATGCCATGTCCATCTACTGAGCAATTTCTTGCTGCTTTAAAACAAGCAGTCCTTGCCAACAAGCGTTGGGTACCACCTTCAGGGAAAGGAGTATTGTATATTAGGCCATTGCTCATTGGAAGTGGTTCTGTTCTTGGCTTGGGGTCAGCAACAGAGTACACATTCCTAATATTTGCTACTCCAATTGGTAGTTATCACAAGAGTGGCAGCACCATGAATTTGTACATTGAGAATGAGGTTCGTAGGGCCACTCCTGGTGGAACTGGAGGCATCAAAAGCATCACCAACTATTCACCAATTTTTAAAACAGTACAGAAGGCTAGGGCCGAAGGGTTCACTGACGTCCTCTTTCTGGATGCAGCCACTGGAAAAAATATTGAGGAGTGTTCTTCATCTAATATTTTCATTGTGAAGGATAATGTGATTTTAACTCCACCAACCAATGGAACTGTCCTTCCAGGGATCACAAGGAAAAGCATCATAGAAATTGCTCTCCATCTCAATTACGTGGTGGAGGAACGTGATGTTTCACTAGAGGATGTGTTGGCTGCTGATGAAGTTTTCTGTACTGGGACTGCTGTGGATGTCATTGCCGTTTCTAGCATAACATACCGTGACAAAAGGGTTGAATACAGAAGAGGGAAAGAAACTGTGTTTTGCAAACTGCGTGAAATTCTTACTGAGATTCAAACAGGTTTGATTGAGGACAAAATGGGGTGGACATTGCTGATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

284

Amino Acids

31.13

Weight (kDa)

8.2

Isoelectric Point (pI)

49.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 11 - 240 4.5e-39 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
Acc65I GGTACC 1 cut(s) 184
AccB1I GGYRCC 1 cut(s) 184
AccB7I CCANNNNNTGG 1 cut(s) 557
AclWI GGATC 1 cut(s) 584
AcsI RAATTY 2 cut(s) 331, 786
AcuI CTGAAG 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 456
AfaI GTAC 5 cut(s) 186, 272, 338, 426, 682
AfiI CCNNNNNNNGG 3 cut(s) 194, 466, 557
AgsI TTSAA 2 cut(s) 743, 803
AjnI CCWGG 2 cut(s) 367, 571
AleI CACNNNNGTG 1 cut(s) 315
AloI GAACNNNNNNTCC 2 cut(s) 553, 585
AluBI AGCT 1 cut(s) 58
AluI AGCT 1 cut(s) 58
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 1 cut(s) 584
AlwNI CAGNNNCTG 1 cut(s) 479
AoxI GGCC 3 cut(s) 218, 360, 438
ApeKI GCWGC 4 cut(s) 146, 321, 473, 662
ApoI RAATTY 2 cut(s) 331, 786
Asp700I GAANNNNTTC 1 cut(s) 238
Asp718I GGTACC 1 cut(s) 184
AspS9I GGNCC 2 cut(s) 360, 438
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 388, 399
BanI GGYRCC 1 cut(s) 184
BbsI GAAGAC 1 cut(s) 98
BbvI GCAGC 4 cut(s) 133, 333, 485, 649
BccI CCATC 3 cut(s) 38, 133, 618
BceAI ACGGC 1 cut(s) 692
BciT130I CCWGG 2 cut(s) 369, 573
BcnI CCSGG 1 cut(s) 68
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 3 cut(s) 435, 647, 714
BisI GCNGC 4 cut(s) 147, 322, 474, 663
BlsI GCNGC 4 cut(s) 148, 323, 475, 664
Bme1390I CCNGG 3 cut(s) 68, 369, 573
BmgT120I GGNCC 2 cut(s) 360, 438
BmiI GGNNCC 1 cut(s) 186
BmrFI CCNGG 3 cut(s) 68, 369, 573
BmrI ACTGGG 1 cut(s) 693
BmsI GCATC 4 cut(s) 393, 402, 460, 600
BmuI ACTGGG 1 cut(s) 693
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 8
BpuMI CCSGG 1 cut(s) 68
BsaAI YACGTR 1 cut(s) 622
BsaHI GRCGYC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 572
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 466, 557
Bse1I ACTGG 3 cut(s) 382, 484, 688
Bse3DI GCAATG 3 cut(s) 221, 702, 836
BseBI CCWGG 2 cut(s) 369, 573
BseDI CCNNGG 1 cut(s) 572
BseGI GGATG 3 cut(s) 475, 658, 703
BseLI CCNNNNNNNGG 3 cut(s) 194, 466, 557
BseMI GCAATG 3 cut(s) 221, 702, 836
BseMII CTCAG 2 cut(s) 123, 786
BseNI ACTGG 3 cut(s) 382, 484, 688
BseRI GAGGAG 1 cut(s) 509
BseXI GCAGC 4 cut(s) 133, 333, 485, 649
BsgI GTGCAG 1 cut(s) 120
BshFI GGCC 3 cut(s) 220, 362, 440
BshNI GGYRCC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 68
BslFI GGGAC 1 cut(s) 700
BslI CCNNNNNNNGG 3 cut(s) 194, 466, 557
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 700
BsmI GAATGC 2 cut(s) 51, 82
BsnI GGCC 3 cut(s) 220, 362, 440
Bso31I GGTCTC 1 cut(s) 26
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 1 cut(s) 576
BspANI GGCC 3 cut(s) 220, 362, 440
BspCNI CTCAG 2 cut(s) 124, 787
BspLI GGNNCC 1 cut(s) 186
BspPI GGATC 1 cut(s) 584
BspT107I GGYRCC 1 cut(s) 184
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 3 cut(s) 221, 702, 836
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 3 cut(s) 382, 484, 688
BssECI CCNNGG 1 cut(s) 572
BssMI GATC 1 cut(s) 576
BssNI GRCGYC 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 369, 573
Bst4CI ACNGT 4 cut(s) 424, 565, 728, 766
Bst6I CTCTTC 2 cut(s) 66, 745
BstACI GRCGYC 1 cut(s) 456
BstAPI GCANNNNNTGC 1 cut(s) 167
BstAUI TGTACA 1 cut(s) 336
BstBAI YACGTR 1 cut(s) 622
BstC8I GCNNGC 1 cut(s) 116
BstDEI CTNAG 3 cut(s) 83, 132, 795
BstF5I GGATG 3 cut(s) 475, 658, 703
BstKTI GATC 1 cut(s) 579
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 576
BstMWI GCNNNNNNNGC 2 cut(s) 167, 390
BstNI CCWGG 2 cut(s) 369, 573
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 3 cut(s) 66, 367, 571
BstV1I GCAGC 4 cut(s) 133, 333, 485, 649
BstV2I GAAGAC 1 cut(s) 98
BsuRI GGCC 3 cut(s) 220, 362, 440
BtsCI GGATG 3 cut(s) 475, 658, 703
BtsIMutI CAGTG 2 cut(s) 450, 477
Cac8I GCNNGC 1 cut(s) 116
CaiI CAGNNNCTG 1 cut(s) 479
Cfr13I GGNCC 2 cut(s) 360, 438
Csp6I GTAC 5 cut(s) 185, 271, 337, 425, 681
CviAII CATG 3 cut(s) 115, 120, 328
CviJI RGCY 8 cut(s) 58, 220, 252, 362, 434, 440, 476, 662
CviKI_1 RGCY 8 cut(s) 58, 220, 252, 362, 434, 440, 476, 662
CviQI GTAC 5 cut(s) 185, 271, 337, 425, 681
DdeI CTNAG 3 cut(s) 83, 132, 795
DpnI GATC 1 cut(s) 578
DpnII GATC 1 cut(s) 576
DraI TTTAAA 2 cut(s) 153, 418
Eam1104I CTCTTC 2 cut(s) 66, 745
EarI CTCTTC 2 cut(s) 66, 745
Eco31I GGTCTC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 367, 571
FaeI CATG 3 cut(s) 118, 123, 331
FaiI YATR 7 cut(s) 116, 121, 213, 329, 596, 719, 724
FalI AAGNNNNNCTT 2 cut(s) 150, 182
FaqI GGGAC 1 cut(s) 700
FatI CATG 3 cut(s) 114, 119, 327
Fnu4HI GCNGC 4 cut(s) 147, 322, 474, 663
FokI GGATG 3 cut(s) 482, 665, 710
Fsp4HI GCNGC 4 cut(s) 147, 322, 474, 663
FspBI CTAG 3 cut(s) 435, 647, 714
GluI GCNGC 4 cut(s) 147, 322, 474, 663
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 3 cut(s) 220, 362, 440
HapII CCGG 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 3 cut(s) 118, 123, 331
HinfI GANTC 1 cut(s) 799
HpaII CCGG 1 cut(s) 68
HphI GGTGA 2 cut(s) 388, 399
Hpy166II GTNNAC 3 cut(s) 273, 450, 834
Hpy188III TCNNGA 2 cut(s) 34, 467
Hpy8I GTNNAC 3 cut(s) 273, 450, 834
HpyAV CCTTC 6 cut(s) 11, 201, 424, 437, 520, 578
HpyCH4III ACNGT 4 cut(s) 424, 565, 728, 766
HpyCH4IV ACGT 3 cut(s) 456, 621, 634
HpyCH4V TGCA 4 cut(s) 101, 114, 473, 774
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 390
HpyF3I CTNAG 3 cut(s) 83, 132, 795
HpySE526I ACGT 3 cut(s) 456, 621, 634
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 3 cut(s) 118, 123, 331
KpnI GGTACC 1 cut(s) 188
Kzo9I GATC 1 cut(s) 576
Lsp1109I GCAGC 4 cut(s) 133, 333, 485, 649
LweI GCATC 4 cut(s) 393, 402, 460, 600
MaeI CTAG 3 cut(s) 435, 647, 714
MaeII ACGT 3 cut(s) 456, 621, 634
MaeIII GTNAC 1 cut(s) 728
MalI GATC 1 cut(s) 578
MboI GATC 1 cut(s) 576
MboII GAAGA 4 cut(s) 83, 103, 495, 762
MfeI CAATTG 1 cut(s) 297
MluCI AATT 7 cut(s) 137, 297, 331, 411, 600, 616, 786
MnlI CCTC 8 cut(s) 343, 374, 470, 487, 622, 643, 746, 811
MroXI GAANNNNTTC 1 cut(s) 238
MseI TTAA 3 cut(s) 152, 417, 542
MslI CAYNNNNRTG 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 1 cut(s) 68
MspR9I CCNGG 3 cut(s) 68, 369, 573
MunI CAATTG 1 cut(s) 297
Mva1269I GAATGC 2 cut(s) 51, 82
MvaI CCWGG 2 cut(s) 369, 573
MwoI GCNNNNNNNGC 2 cut(s) 167, 390
NciI CCSGG 1 cut(s) 68
NdeII GATC 1 cut(s) 576
NlaIII CATG 3 cut(s) 118, 123, 331
NlaIV GGNNCC 1 cut(s) 186
NmuCI GTSAC 1 cut(s) 728
NspI RCATGY 1 cut(s) 118
OliI CACNNNNGTG 1 cut(s) 315
PaeI GCATGC 1 cut(s) 118
PctI GAATGC 2 cut(s) 51, 82
PdmI GAANNNNTTC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 799
PflMI CCANNNNNTGG 1 cut(s) 557
PkrI GCNGC 4 cut(s) 148, 323, 475, 664
Ppu21I YACGTR 1 cut(s) 622
Psp6I CCWGG 2 cut(s) 367, 571
PspGI CCWGG 2 cut(s) 367, 571
PspN4I GGNNCC 1 cut(s) 186
PspPI GGNCC 2 cut(s) 360, 438
PstNI CAGNNNCTG 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 5 cut(s) 186, 272, 338, 426, 682
RsaNI GTAC 5 cut(s) 185, 271, 337, 425, 681
RseI CAYNNNNRTG 1 cut(s) 315
SaqAI TTAA 3 cut(s) 152, 417, 542
SatI GCNGC 4 cut(s) 147, 322, 474, 663
Sau3AI GATC 1 cut(s) 576
Sau96I GGNCC 2 cut(s) 360, 438
ScrFI CCNGG 3 cut(s) 68, 369, 573
SetI ASST 8 cut(s) 22, 60, 193, 354, 459, 624, 637, 811
SfaNI GCATC 4 cut(s) 393, 402, 460, 600
SmiMI CAYNNNNRTG 1 cut(s) 315
SmlI CTYRAG 1 cut(s) 23
SmoI CTYRAG 1 cut(s) 23
SphI GCATGC 1 cut(s) 118
Sse9I AATT 7 cut(s) 137, 297, 331, 411, 600, 616, 786
SspI AATATT 4 cut(s) 12, 285, 490, 514
SspMI CTAG 3 cut(s) 435, 647, 714
StyD4I CCNGG 3 cut(s) 66, 367, 571
TaaI ACNGT 4 cut(s) 424, 565, 728, 766
TaiI ACGT 3 cut(s) 459, 624, 637
TasI AATT 7 cut(s) 137, 297, 331, 411, 600, 616, 786
TatI WGTACW 4 cut(s) 270, 336, 424, 680
TfiI GAWTC 1 cut(s) 799
Tru1I TTAA 3 cut(s) 152, 417, 542
Tru9I TTAA 3 cut(s) 152, 417, 542
TscAI CASTG 2 cut(s) 457, 484
TseFI GTSAC 1 cut(s) 728
TseI GCWGC 4 cut(s) 146, 321, 473, 662
Tsp45I GTSAC 1 cut(s) 728
TspDTI ATGAA 5 cut(s) 104, 344, 495, 508, 684
TspRI CASTG 2 cut(s) 457, 484
Van91I CCANNNNNTGG 1 cut(s) 557
XapI RAATTY 2 cut(s) 331, 786
XceI RCATGY 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 238
XspI CTAG 3 cut(s) 435, 647, 714
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.