MD10G1114000.v1.1

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0002089
Physical Location & Seq
Forward (+)
18710720 .. 18714625
3906 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1114000.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGCCTCCTTCTTTGCAAAAGATTTCTCAATCTGTCGAAAGTCGCAGTGCGGATGAAAAGTACGCTGATGTGAACTGGGATGAGCTTGGATTTGGAGTAATTCCCACTGAATTCATGTACATCATGAAATGTTCCAATGGAGGTGAATTCTCACAAGGCCATCTCACTCCCTATCGAAACATTGAGCTCAGCCCATCTGCTGGAGTCTTGAATTATGGACAGGGTTTATTCGAAGGTCTTAAGGCGTACAGGACAGAAGATGATCGATTTCTGCTCTTTAGGCCTGAAGAGAATGCTTTAAGAATGCAGATGGGAGCAGAGAGAATGTTAATGCCATCACCATCTACTCAACAATTCCTGGATGCTGTGAAGCAAACAGTCCTTGCCAATAAGCGTTGGATACCACCTCTAGGGAAAGGAACGCTGTATGTTAGGCCGTTGCTCATTGGAAGCGGACCTGTTCTTGGCGTGGCACCGGCAGCAGAATACACATTCCTCGTATTTGCTGCTCCAGTCGGTATTTATCACAAGGGCCCGGCAGGCCTGAACTTGTATGTCGAGGATCAACTGCATAGAGCTACTCCTGGTGGAACTGGAGGTGTCAAGTCCATCACCAACTATTCGCCGGCTTACCTAGCACAACAACAAGCAAGGGCCAAAGGGTTTTCTGATGTCCTATTCCTGGATTCCGTGACCGGAAAATACATAGAGGAGCTGACAGCATGTAATATTTTCATTCTGAAGGGTAATGTTCTTGCAACTCCAGCAGTTCATGGAACTATTCTTCCTGGAATCACTCGTAAAAGCATCATCGCCATTGCCCTCGATTTTGGATACCAGGTAGAGGAACGTGTAATTCCGGTGGAGGATTTGCTTGAAGCTGACGAAGCTTTCTGCACAGGAACTGCGGTTGTTGTCACCCCAATTGGTGCTGCAACTTATCAAGGCAAAAGGGTTGAGTACAACACAGGAAAAGGAACACTCTTTCACAAACTGCGTGAAACCCTAACAGGAATCCAAACAGGGCGTCTGGAAGACAAGAAGGGATGGACCATGGAGATCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

356

Amino Acids

38.85

Weight (kDa)

6.03

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 75 - 309 4.2e-35 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 472
AccB7I CCANNNNNTGG 1 cut(s) 200
AciI CCGC 3 cut(s) 50, 453, 908
AclWI GGATC 1 cut(s) 570
AcsI RAATTY 2 cut(s) 110, 146
AcuI CTGAAG 2 cut(s) 306, 761
AcyI GRCGYC 1 cut(s) 1027
AfaI GTAC 4 cut(s) 62, 119, 248, 962
AfiI CCNNNNNNNGG 5 cut(s) 200, 410, 464, 682, 844
AflII CTTAAG 1 cut(s) 239
AflIII ACRYGT 1 cut(s) 850
AgsI TTSAA 2 cut(s) 211, 878
AjnI CCWGG 5 cut(s) 357, 583, 681, 787, 837
AloI GAACNNNNNNTCC 2 cut(s) 769, 801
AluBI AGCT 6 cut(s) 85, 187, 578, 715, 881, 890
AluI AGCT 6 cut(s) 85, 187, 578, 715, 881, 890
Alw21I GWGCWC 1 cut(s) 189
AlwI GGATC 1 cut(s) 570
AlwNI CAGNNNCTG 1 cut(s) 905
AoxI GGCC 6 cut(s) 157, 281, 434, 532, 541, 654
ApaI GGGCCC 1 cut(s) 536
ApeKI GCWGC 3 cut(s) 479, 506, 932
ApoI RAATTY 2 cut(s) 110, 146
ArsI GACNNNNNNTTYG 2 cut(s) 1012, 1044
AspS9I GGNCC 5 cut(s) 455, 532, 533, 654, 1050
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 4 cut(s) 155, 330, 604, 910
AsuII TTCGAA 1 cut(s) 231
AvaII GGWCC 2 cut(s) 455, 1050
BaeGI GKGCMC 1 cut(s) 536
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 2 cut(s) 189, 536
BbsI GAAGAC 1 cut(s) 1041
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 3 cut(s) 491, 493, 919
BccI CCATC 7 cut(s) 168, 202, 304, 343, 349, 617, 1041
BceAI ACGGC 1 cut(s) 421
BciT130I CCWGG 5 cut(s) 359, 585, 683, 789, 839
BciVI GTATCC 2 cut(s) 393, 827
BcnI CCSGG 1 cut(s) 536
BfaI CTAG 2 cut(s) 410, 635
BfrI CTTAAG 1 cut(s) 239
BfuI GTATCC 2 cut(s) 393, 827
BglI GCCNNNNNGGC 1 cut(s) 540
BisI GCNGC 3 cut(s) 480, 507, 933
BlpI GCTNAGC 1 cut(s) 188
BlsI GCNGC 3 cut(s) 481, 508, 934
Bme1390I CCNGG 6 cut(s) 359, 536, 585, 683, 789, 839
Bme18I GGWCC 2 cut(s) 455, 1050
BmgT120I GGNCC 5 cut(s) 455, 532, 533, 654, 1050
BmiI GGNNCC 2 cut(s) 474, 534
BmrFI CCNGG 6 cut(s) 359, 536, 585, 683, 789, 839
BmrI ACTGGG 1 cut(s) 85
BmsI GCATC 2 cut(s) 352, 816
BmuI ACTGGG 1 cut(s) 85
BpiI GAAGAC 1 cut(s) 1041
BpmI CTGGAG 4 cut(s) 222, 495, 615, 747
Bpu1102I GCTNAGC 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 231
BpuMI CCSGG 1 cut(s) 536
Bsa29I ATCGAT 1 cut(s) 265
BsaHI GRCGYC 1 cut(s) 1027
BsaJI CCNNGG 1 cut(s) 1053
BsaWI WCCGGW 2 cut(s) 695, 859
Bsc4I CCNNNNNNNGG 5 cut(s) 200, 410, 464, 682, 844
Bse118I RCCGGY 2 cut(s) 475, 625
Bse1I ACTGG 3 cut(s) 80, 512, 598
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 5 cut(s) 359, 585, 683, 789, 839
BseCI ATCGAT 1 cut(s) 265
BseDI CCNNGG 1 cut(s) 1053
BseGI GGATG 4 cut(s) 58, 85, 367, 1052
BseLI CCNNNNNNNGG 5 cut(s) 200, 410, 464, 682, 844
BseMI GCAATG 1 cut(s) 816
BseMII CTCAG 1 cut(s) 202
BseNI ACTGG 3 cut(s) 80, 512, 598
BseRI GAGGAG 1 cut(s) 725
BseSI GKGCMC 1 cut(s) 536
BseXI GCAGC 3 cut(s) 491, 493, 919
BsgI GTGCAG 1 cut(s) 880
BshFI GGCC 6 cut(s) 159, 283, 436, 534, 543, 656
BshNI GGYRCC 1 cut(s) 472
BshVI ATCGAT 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 5 cut(s) 476, 536, 626, 696, 860
BslI CCNNNNNNNGG 5 cut(s) 200, 410, 464, 682, 844
BsmI GAATGC 2 cut(s) 298, 309
BsnI GGCC 6 cut(s) 159, 283, 436, 534, 543, 656
Bsp119I TTCGAA 1 cut(s) 231
Bsp120I GGGCCC 1 cut(s) 532
Bsp1286I GDGCHC 2 cut(s) 189, 536
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 3 cut(s) 262, 562, 1059
Bsp1720I GCTNAGC 1 cut(s) 188
Bsp19I CCATGG 1 cut(s) 1053
BspACI CCGC 3 cut(s) 50, 453, 908
BspANI GGCC 6 cut(s) 159, 283, 436, 534, 543, 656
BspCNI CTCAG 1 cut(s) 201
BspDI ATCGAT 1 cut(s) 265
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 474, 534
BspPI GGATC 1 cut(s) 570
BspT104I TTCGAA 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 472
BspTI CTTAAG 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 816
BsrFI RCCGGY 2 cut(s) 475, 625
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 3 cut(s) 80, 512, 598
BssAI RCCGGY 2 cut(s) 475, 625
BssECI CCNNGG 1 cut(s) 1053
BssMI GATC 3 cut(s) 262, 562, 1059
BssNI GRCGYC 1 cut(s) 1027
BssT1I CCWWGG 1 cut(s) 1053
Bst2UI CCWGG 5 cut(s) 359, 585, 683, 789, 839
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 1 cut(s) 1027
BstAFI CTTAAG 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 541, 627
BstDEI CTNAG 1 cut(s) 188
BstDSI CCRYGG 1 cut(s) 1053
BstF5I GGATG 4 cut(s) 58, 85, 367, 1052
BstKTI GATC 3 cut(s) 265, 565, 1062
BstMBI GATC 3 cut(s) 262, 562, 1059
BstMWI GCNNNNNNNGC 6 cut(s) 280, 479, 540, 635, 764, 887
BstNI CCWGG 5 cut(s) 359, 585, 683, 789, 839
BstNSI RCATGY 1 cut(s) 726
BstSCI CCNGG 6 cut(s) 357, 534, 583, 681, 787, 837
BstSLI GKGCMC 1 cut(s) 536
BstV1I GCAGC 3 cut(s) 491, 493, 919
BstV2I GAAGAC 1 cut(s) 1041
Bsu15I ATCGAT 1 cut(s) 265
BsuI GTATCC 2 cut(s) 393, 827
BsuRI GGCC 6 cut(s) 159, 283, 436, 534, 543, 656
BsuTUI ATCGAT 1 cut(s) 265
BtgI CCRYGG 1 cut(s) 1053
BtgZI GCGATG 1 cut(s) 796
BtsCI GGATG 4 cut(s) 58, 85, 367, 1052
BtsI GCAGTG 1 cut(s) 52
BtsIMutI CAGTG 2 cut(s) 52, 105
Cac8I GCNNGC 2 cut(s) 541, 627
CaiI CAGNNNCTG 1 cut(s) 905
CciI TCATGA 1 cut(s) 123
Cfr10I RCCGGY 2 cut(s) 475, 625
Cfr13I GGNCC 5 cut(s) 455, 532, 533, 654, 1050
ClaI ATCGAT 1 cut(s) 265
CseI GACGC 1 cut(s) 1016
CsiI ACCWGGT 1 cut(s) 837
Csp6I GTAC 4 cut(s) 61, 118, 247, 961
CviAII CATG 5 cut(s) 115, 124, 723, 773, 1054
CviQI GTAC 4 cut(s) 61, 118, 247, 961
DdeI CTNAG 1 cut(s) 188
DpnI GATC 3 cut(s) 264, 564, 1061
DpnII GATC 3 cut(s) 262, 562, 1059
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 1053
Eco147I AGGCCT 2 cut(s) 283, 543
Eco24I GRGCYC 2 cut(s) 189, 536
Eco47I GGWCC 2 cut(s) 455, 1050
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 2 cut(s) 306, 761
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 532
EcoRI GAATTC 2 cut(s) 110, 146
EcoRII CCWGG 5 cut(s) 357, 583, 681, 787, 837
EcoT14I CCWWGG 1 cut(s) 1053
EcoT38I GRGCYC 2 cut(s) 189, 536
ErhI CCWWGG 1 cut(s) 1053
FaeI CATG 5 cut(s) 118, 127, 726, 776, 1057
FatI CATG 5 cut(s) 114, 123, 722, 772, 1053
Fnu4HI GCNGC 3 cut(s) 480, 507, 933
FokI GGATG 4 cut(s) 65, 92, 374, 1059
FriOI GRGCYC 2 cut(s) 189, 536
Fsp4HI GCNGC 3 cut(s) 480, 507, 933
FspBI CTAG 2 cut(s) 410, 635
GluI GCNGC 3 cut(s) 480, 507, 933
GsuI CTGGAG 4 cut(s) 222, 495, 615, 747
HaeIII GGCC 6 cut(s) 159, 283, 436, 534, 543, 656
HapII CCGG 5 cut(s) 476, 536, 626, 696, 860
HgaI GACGC 1 cut(s) 1016
Hin1I GRCGYC 1 cut(s) 1027
Hin1II CATG 5 cut(s) 118, 127, 726, 776, 1057
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 4 cut(s) 204, 686, 792, 1014
HpaII CCGG 5 cut(s) 476, 536, 626, 696, 860
HphI GGTGA 4 cut(s) 155, 330, 604, 910
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 2 cut(s) 670, 741
Hpy188III TCNNGA 3 cut(s) 124, 208, 1031
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 18, 227, 736, 1036
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 850
HpyCH4V TGCA 6 cut(s) 16, 307, 571, 758, 897, 935
HpyF10VI GCNNNNNNNGC 6 cut(s) 280, 479, 540, 635, 764, 887
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 850
Hsp92I GRCGYC 1 cut(s) 1027
Hsp92II CATG 5 cut(s) 118, 127, 726, 776, 1057
KroI GCCGGC 1 cut(s) 625
KroNI GCCGGC 1 cut(s) 627
Kzo9I GATC 3 cut(s) 262, 562, 1059
LmnI GCTCC 3 cut(s) 314, 514, 712
Lsp1109I GCAGC 3 cut(s) 491, 493, 919
LweI GCATC 2 cut(s) 352, 816
MabI ACCWGGT 1 cut(s) 837
MaeI CTAG 2 cut(s) 410, 635
MaeII ACGT 1 cut(s) 850
MaeIII GTNAC 2 cut(s) 691, 916
MalI GATC 3 cut(s) 264, 564, 1061
MboI GATC 3 cut(s) 262, 562, 1059
MboII GAAGA 4 cut(s) 269, 299, 776, 1046
MfeI CAATTG 2 cut(s) 924, 1063
MhlI GDGCHC 2 cut(s) 189, 536
MluCI AATT 8 cut(s) 99, 110, 146, 211, 353, 855, 924, 1063
MlyI GAGTC 1 cut(s) 213
MmeI TCCRAC 1 cut(s) 377
MroNI GCCGGC 1 cut(s) 625
MseI TTAA 3 cut(s) 240, 299, 329
MspCI CTTAAG 1 cut(s) 239
MspI CCGG 5 cut(s) 476, 536, 626, 696, 860
MspR9I CCNGG 6 cut(s) 359, 536, 585, 683, 789, 839
MunI CAATTG 2 cut(s) 924, 1063
Mva1269I GAATGC 2 cut(s) 298, 309
MvaI CCWGG 5 cut(s) 359, 585, 683, 789, 839
MwoI GCNNNNNNNGC 6 cut(s) 280, 479, 540, 635, 764, 887
NaeI GCCGGC 1 cut(s) 627
NciI CCSGG 1 cut(s) 536
NcoI CCATGG 1 cut(s) 1053
NdeII GATC 3 cut(s) 262, 562, 1059
NgoMIV GCCGGC 1 cut(s) 625
NlaIII CATG 5 cut(s) 118, 127, 726, 776, 1057
NlaIV GGNNCC 2 cut(s) 474, 534
NmuCI GTSAC 2 cut(s) 691, 916
NspI RCATGY 1 cut(s) 726
NspV TTCGAA 1 cut(s) 231
PagI TCATGA 1 cut(s) 123
PceI AGGCCT 2 cut(s) 283, 543
PctI GAATGC 2 cut(s) 298, 309
PdiI GCCGGC 1 cut(s) 627
PfeI GAWTC 3 cut(s) 686, 792, 1014
PflMI CCANNNNNTGG 1 cut(s) 200
PfoI TCCNGGA 3 cut(s) 357, 681, 787
PkrI GCNGC 3 cut(s) 481, 508, 934
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 5 cut(s) 357, 583, 681, 787, 837
PspGI CCWGG 5 cut(s) 357, 583, 681, 787, 837
PspN4I GGNNCC 2 cut(s) 474, 534
PspOMI GGGCCC 1 cut(s) 532
PspPI GGNCC 5 cut(s) 455, 532, 533, 654, 1050
PstNI CAGNNNCTG 1 cut(s) 905
RsaI GTAC 4 cut(s) 62, 119, 248, 962
RsaNI GTAC 4 cut(s) 61, 118, 247, 961
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 3 cut(s) 240, 299, 329
SatI GCNGC 3 cut(s) 480, 507, 933
Sau3AI GATC 3 cut(s) 262, 562, 1059
Sau96I GGNCC 5 cut(s) 455, 532, 533, 654, 1050
SchI GAGTC 1 cut(s) 213
ScrFI CCNGG 6 cut(s) 359, 536, 585, 683, 789, 839
SduI GDGCHC 2 cut(s) 189, 536
SexAI ACCWGGT 1 cut(s) 837
SfaNI GCATC 2 cut(s) 352, 816
SfiI GGCCNNNNNGGCC 1 cut(s) 540
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 455, 1050
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 8 cut(s) 99, 110, 146, 211, 353, 855, 924, 1063
SseBI AGGCCT 2 cut(s) 283, 543
SsiI CCGC 3 cut(s) 50, 453, 908
SspI AATATT 1 cut(s) 730
SspMI CTAG 2 cut(s) 410, 635
SstI GAGCTC 1 cut(s) 189
StuI AGGCCT 2 cut(s) 283, 543
StyD4I CCNGG 6 cut(s) 357, 534, 583, 681, 787, 837
StyI CCWWGG 1 cut(s) 1053
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 853
TaqI TCGA 6 cut(s) 36, 175, 231, 265, 558, 825
TasI AATT 8 cut(s) 99, 110, 146, 211, 353, 855, 924, 1063
TatI WGTACW 2 cut(s) 117, 960
TfiI GAWTC 3 cut(s) 686, 792, 1014
Tru1I TTAA 3 cut(s) 240, 299, 329
Tru9I TTAA 3 cut(s) 240, 299, 329
TscAI CASTG 2 cut(s) 52, 112
TseFI GTSAC 2 cut(s) 691, 916
TseI GCWGC 3 cut(s) 479, 506, 932
Tsp45I GTSAC 2 cut(s) 691, 916
TspDTI ATGAA 5 cut(s) 69, 103, 140, 724, 761
TspGWI ACGGA 1 cut(s) 679
TspRI CASTG 2 cut(s) 52, 112
Van91I CCANNNNNTGG 1 cut(s) 200
Vha464I CTTAAG 1 cut(s) 239
VpaK11BI GGWCC 2 cut(s) 455, 1050
XapI RAATTY 2 cut(s) 110, 146
XceI RCATGY 1 cut(s) 726
XspI CTAG 2 cut(s) 410, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.