Rh6DG032500

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0002089
Physical Location & Seq
Reverse (-)
3193127 .. 3196131
3005 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG032500.1

Sequence Viewer

Length: 1080 bp
ATGTCTTCTTCAGCTTTGCAAACAACTGGTGAATCTAACAACCACAGTCTGAAAAACGGTGAAATGTATGCTGATGGCATTAACTGGGATGATCTTGGGTTTGGACTGATTCCAATCGATTACATGTACACCATGAAATGCTCCGACGGAGAAAACTTTTCACAAGGACATCTCTCTCCCTTTGGAAAGATTGAGCTTTGCCCATCTGCTGGAATATTGAATTATGGACAGGGACTATTCGAAGGTCTTAAGGCGACCAGAACAGAAGATGGTCGTATTATGCTGTTTCGGCCCCAAGAGAATGCTCTAAGAATGAAGATGGGTGCAGAGAGAATGTGCATGCCATCTCCATCCGTTGAGCAATTTGTTGATGCTGTGAAGCAAACAGTCTTCGCCAACAAGCGTTGGGTACCACCTCCAGGAAAAGGATCATTGTATATTAGGCCTTTGCTTATGGGGAGTGGATCTAATCTGGGCTTGGGGCCGGGACTGGAGTATACATTCCTAATATTTGCTTCTCCAGTTGGTAATAATTATCACAAGGGAAGGGCAGCCCTCAATTTGTATGTGGAGCATAAAATCCACAGAGCTACTCCTGGTGGAACTGGAGGTGTCAAGTCCGTCACCAATTACTCACCTGTTTACCAAACACAACAGCAAGCAAGGGCCAAGGGGTTCTCTGATGTCTTATTCCTAGATTCTGCAACTGGTAAAAACATTGAGGAGATTGTAGCAGCTAACATTTTCATTCTGAAGGGTAATGTCATTTCAACTCCAACACTGAAACATGGAACTATCCTCCCTGGAGTAACCCGTAAAAGCATCATTGACATTGCCCTTGATTTTGGTTACCAGGTGGAGGAGCGTGTGATCCCAGTGGAGGATTTACTTGCAGCCGATGAAGCTTTCTGCACAGGAACTGCCGTGGTTGTCAACCCTATTGGCGCTGTAACTTATCAAGACAAGAGGGTTGAATACAAAACAGGAGGAGCATTATGTCAGAAACTGTATGAAACGCTAACAGGAATCCAAACAGGTCGTCTTGAGGACAAGAAGGGATGGACCCTAGAGGTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

359

Amino Acids

39.12

Weight (kDa)

6.61

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 78 - 315 4.2e-32 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 409
AccB7I CCANNNNNTGG 1 cut(s) 209
AccI GTMKAC 1 cut(s) 497
AclWI GGATC 3 cut(s) 436, 472, 865
AcuI CTGAAG 1 cut(s) 773
AfaI GTAC 2 cut(s) 128, 411
AfiI CCNNNNNNNGG 5 cut(s) 209, 419, 425, 859, 880
AflII CTTAAG 1 cut(s) 248
AflIII ACRYGT 1 cut(s) 123
AgsI TTSAA 3 cut(s) 220, 771, 974
AjnI CCWGG 4 cut(s) 418, 595, 802, 852
AluBI AGCT 5 cut(s) 14, 196, 590, 737, 905
AluI AGCT 5 cut(s) 14, 196, 590, 737, 905
AlwI GGATC 3 cut(s) 436, 472, 865
AlwNI CAGNNNCTG 2 cut(s) 920, 1006
AoxI GGCC 4 cut(s) 290, 443, 482, 666
ApeKI GCWGC 3 cut(s) 551, 734, 893
ArsI GACNNNNNNTTYG 2 cut(s) 1024, 1056
Asp718I GGTACC 1 cut(s) 409
AspLEI GCGC 1 cut(s) 947
AspS9I GGNCC 4 cut(s) 291, 482, 666, 1062
AsuC2I CCSGG 1 cut(s) 486
AsuHPI GGTGA 4 cut(s) 41, 71, 616, 627
AsuII TTCGAA 1 cut(s) 240
AvaII GGWCC 1 cut(s) 1062
BanI GGYRCC 1 cut(s) 409
BbsI GAAGAC 1 cut(s) 382
BbvI GCAGC 3 cut(s) 563, 746, 905
BccI CCATC 7 cut(s) 68, 211, 263, 313, 352, 358, 1053
BceAI ACGGC 1 cut(s) 908
BciT130I CCWGG 4 cut(s) 420, 597, 804, 854
BcnI CCSGG 1 cut(s) 486
BfaI CTAG 2 cut(s) 695, 1067
BfoI RGCGCY 1 cut(s) 948
BfrI CTTAAG 1 cut(s) 248
BisI GCNGC 3 cut(s) 552, 735, 894
BlsI GCNGC 3 cut(s) 553, 736, 895
Bme1390I CCNGG 5 cut(s) 420, 486, 597, 804, 854
Bme18I GGWCC 1 cut(s) 1062
BmgT120I GGNCC 4 cut(s) 291, 482, 666, 1062
BmiI GGNNCC 4 cut(s) 293, 411, 483, 1064
BmrFI CCNGG 5 cut(s) 420, 486, 597, 804, 854
BmrI ACTGGG 2 cut(s) 94, 869
BmsI GCATC 2 cut(s) 361, 831
BmuI ACTGGG 2 cut(s) 94, 869
BpiI GAAGAC 1 cut(s) 382
BpmI CTGGAG 5 cut(s) 402, 504, 512, 627, 825
Bpu14I TTCGAA 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 1064
BpuMI CCSGG 1 cut(s) 486
Bsa29I ATCGAT 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 113
BsaJI CCNNGG 3 cut(s) 669, 802, 924
Bsc4I CCNNNNNNNGG 5 cut(s) 209, 419, 425, 859, 880
Bse1I ACTGG 7 cut(s) 31, 89, 495, 521, 610, 712, 875
Bse3DI GCAATG 1 cut(s) 831
Bse8I GATNNNNATC 1 cut(s) 113
BseBI CCWGG 4 cut(s) 420, 597, 804, 854
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 3 cut(s) 669, 802, 924
BseGI GGATG 3 cut(s) 94, 350, 1064
BseJI GATNNNNATC 1 cut(s) 113
BseLI CCNNNNNNNGG 5 cut(s) 209, 419, 425, 859, 880
BseMI GCAATG 1 cut(s) 831
BseNI ACTGG 7 cut(s) 31, 89, 495, 521, 610, 712, 875
BseRI GAGGAG 3 cut(s) 737, 875, 1002
BseXI GCAGC 3 cut(s) 563, 746, 905
BsgI GTGCAG 2 cut(s) 345, 895
BshFI GGCC 4 cut(s) 292, 445, 484, 668
BshNI GGYRCC 1 cut(s) 409
BshVI ATCGAT 1 cut(s) 117
BsiSI CCGG 1 cut(s) 485
BslFI GGGAC 2 cut(s) 246, 501
BslI CCNNNNNNNGG 5 cut(s) 209, 419, 425, 859, 880
BsmFI GGGAC 2 cut(s) 246, 501
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 4 cut(s) 292, 445, 484, 668
Bsp119I TTCGAA 1 cut(s) 240
Bsp1407I TGTACA 1 cut(s) 126
Bsp143I GATC 4 cut(s) 91, 428, 464, 870
BspANI GGCC 4 cut(s) 292, 445, 484, 668
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 4 cut(s) 293, 411, 483, 1064
BspPI GGATC 3 cut(s) 436, 472, 865
BspT104I TTCGAA 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 409
BspTI CTTAAG 1 cut(s) 248
BsrDI GCAATG 1 cut(s) 831
BsrGI TGTACA 1 cut(s) 126
BsrI ACTGG 7 cut(s) 31, 89, 495, 521, 610, 712, 875
BssECI CCNNGG 3 cut(s) 669, 802, 924
BssMI GATC 4 cut(s) 91, 428, 464, 870
BssNAI GTATAC 1 cut(s) 498
BssT1I CCWWGG 1 cut(s) 669
Bst1107I GTATAC 1 cut(s) 498
Bst2UI CCWGG 4 cut(s) 420, 597, 804, 854
Bst4CI ACNGT 4 cut(s) 47, 59, 388, 1008
BstAFI CTTAAG 1 cut(s) 248
BstAUI TGTACA 1 cut(s) 126
BstBI TTCGAA 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 341, 660
BstDEI CTNAG 1 cut(s) 308
BstDSI CCRYGG 1 cut(s) 924
BstEII GGTNACC 1 cut(s) 848
BstF5I GGATG 3 cut(s) 94, 350, 1064
BstH2I RGCGCY 1 cut(s) 948
BstHHI GCGC 1 cut(s) 947
BstKTI GATC 4 cut(s) 94, 431, 467, 873
BstMBI GATC 4 cut(s) 91, 428, 464, 870
BstMWI GCNNNNNNNGC 2 cut(s) 289, 902
BstNI CCWGG 4 cut(s) 420, 597, 804, 854
BstNSI RCATGY 2 cut(s) 127, 343
BstPI GGTNACC 1 cut(s) 848
BstSCI CCNGG 5 cut(s) 418, 484, 595, 802, 852
BstV1I GCAGC 3 cut(s) 563, 746, 905
BstV2I GAAGAC 1 cut(s) 382
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
BstZ17I GTATAC 1 cut(s) 498
Bsu15I ATCGAT 1 cut(s) 117
BsuRI GGCC 4 cut(s) 292, 445, 484, 668
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 924
BtsCI GGATG 3 cut(s) 94, 350, 1064
BtsIMutI CAGTG 2 cut(s) 779, 882
Cac8I GCNNGC 2 cut(s) 341, 660
CaiI CAGNNNCTG 2 cut(s) 920, 1006
CfoI GCGC 1 cut(s) 947
Cfr13I GGNCC 4 cut(s) 291, 482, 666, 1062
ClaI ATCGAT 1 cut(s) 117
CsiI ACCWGGT 1 cut(s) 852
Csp6I GTAC 2 cut(s) 127, 410
CviAII CATG 4 cut(s) 124, 133, 340, 788
CviQI GTAC 2 cut(s) 127, 410
DdeI CTNAG 1 cut(s) 308
DpnI GATC 4 cut(s) 93, 430, 466, 872
DpnII GATC 4 cut(s) 91, 428, 464, 870
Eco130I CCWWGG 1 cut(s) 669
Eco147I AGGCCT 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 1062
Eco57I CTGAAG 1 cut(s) 773
Eco91I GGTNACC 1 cut(s) 848
EcoO65I GGTNACC 1 cut(s) 848
EcoRII CCWGG 4 cut(s) 418, 595, 802, 852
EcoT14I CCWWGG 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 669
FaeI CATG 4 cut(s) 127, 136, 343, 791
FaqI GGGAC 2 cut(s) 246, 501
FatI CATG 4 cut(s) 123, 132, 339, 787
FblI GTMKAC 1 cut(s) 497
Fnu4HI GCNGC 3 cut(s) 552, 735, 894
FokI GGATG 3 cut(s) 101, 337, 1071
Fsp4HI GCNGC 3 cut(s) 552, 735, 894
FspBI CTAG 2 cut(s) 695, 1067
GlaI GCGC 1 cut(s) 946
GluI GCNGC 3 cut(s) 552, 735, 894
GsuI CTGGAG 5 cut(s) 402, 504, 512, 627, 825
HaeII RGCGCY 1 cut(s) 948
HaeIII GGCC 4 cut(s) 292, 445, 484, 668
HapII CCGG 1 cut(s) 485
HhaI GCGC 1 cut(s) 947
Hin1II CATG 4 cut(s) 127, 136, 343, 791
Hin6I GCGC 1 cut(s) 945
HinP1I GCGC 1 cut(s) 945
HincII GTYRAC 2 cut(s) 934, 1075
HindII GTYRAC 2 cut(s) 934, 1075
HindIII AAGCTT 1 cut(s) 903
HinfI GANTC 4 cut(s) 32, 109, 698, 1026
HpaII CCGG 1 cut(s) 485
HphI GGTGA 4 cut(s) 41, 71, 616, 627
Hpy166II GTNNAC 5 cut(s) 129, 498, 643, 934, 1075
Hpy188I TCNGA 5 cut(s) 51, 145, 682, 753, 1002
Hpy188III TCNNGA 2 cut(s) 959, 1043
Hpy8I GTNNAC 5 cut(s) 129, 498, 643, 934, 1075
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 4 cut(s) 236, 540, 748, 1048
HpyCH4III ACNGT 4 cut(s) 47, 59, 388, 1008
HpyCH4V TGCA 6 cut(s) 19, 326, 339, 704, 893, 912
HpyF10VI GCNNNNNNNGC 2 cut(s) 289, 902
HpyF3I CTNAG 1 cut(s) 308
Hsp92II CATG 4 cut(s) 127, 136, 343, 791
HspAI GCGC 1 cut(s) 945
KpnI GGTACC 1 cut(s) 413
Kzo9I GATC 4 cut(s) 91, 428, 464, 870
LmnI GCTCC 4 cut(s) 146, 571, 862, 989
Lsp1109I GCAGC 3 cut(s) 563, 746, 905
LweI GCATC 2 cut(s) 361, 831
MabI ACCWGGT 1 cut(s) 852
MaeI CTAG 2 cut(s) 695, 1067
MaeIII GTNAC 4 cut(s) 622, 808, 848, 949
MalI GATC 4 cut(s) 93, 430, 466, 872
MboI GATC 4 cut(s) 91, 428, 464, 870
MboII GAAGA 3 cut(s) 278, 328, 382
MflI RGATCY 1 cut(s) 464
MluCI AATT 5 cut(s) 220, 362, 532, 559, 628
MmeI TCCRAC 2 cut(s) 168, 800
MseI TTAA 2 cut(s) 81, 249
MspCI CTTAAG 1 cut(s) 248
MspI CCGG 1 cut(s) 485
MspR9I CCNGG 5 cut(s) 420, 486, 597, 804, 854
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 4 cut(s) 420, 597, 804, 854
MwoI GCNNNNNNNGC 2 cut(s) 289, 902
NciI CCSGG 1 cut(s) 486
NdeII GATC 4 cut(s) 91, 428, 464, 870
NlaIII CATG 4 cut(s) 127, 136, 343, 791
NlaIV GGNNCC 4 cut(s) 293, 411, 483, 1064
NmuCI GTSAC 1 cut(s) 622
NspI RCATGY 2 cut(s) 127, 343
NspV TTCGAA 1 cut(s) 240
PaeI GCATGC 1 cut(s) 343
PceI AGGCCT 1 cut(s) 445
PciI ACATGT 1 cut(s) 123
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 4 cut(s) 32, 109, 698, 1026
PflMI CCANNNNNTGG 1 cut(s) 209
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 3 cut(s) 553, 736, 895
PscI ACATGT 1 cut(s) 123
Psp6I CCWGG 4 cut(s) 418, 595, 802, 852
PspEI GGTNACC 1 cut(s) 848
PspGI CCWGG 4 cut(s) 418, 595, 802, 852
PspN4I GGNNCC 4 cut(s) 293, 411, 483, 1064
PspPI GGNCC 4 cut(s) 291, 482, 666, 1062
PstNI CAGNNNCTG 2 cut(s) 920, 1006
PsuI RGATCY 1 cut(s) 464
RsaI GTAC 2 cut(s) 128, 411
RsaNI GTAC 2 cut(s) 127, 410
SaqAI TTAA 2 cut(s) 81, 249
SatI GCNGC 3 cut(s) 552, 735, 894
Sau3AI GATC 4 cut(s) 91, 428, 464, 870
Sau96I GGNCC 4 cut(s) 291, 482, 666, 1062
ScrFI CCNGG 5 cut(s) 420, 486, 597, 804, 854
SexAI ACCWGGT 1 cut(s) 852
SfaNI GCATC 2 cut(s) 361, 831
SfuI TTCGAA 1 cut(s) 240
SinI GGWCC 1 cut(s) 1062
SmlI CTYRAG 2 cut(s) 248, 1043
SmoI CTYRAG 2 cut(s) 248, 1043
SphI GCATGC 1 cut(s) 343
Sse9I AATT 5 cut(s) 220, 362, 532, 559, 628
SseBI AGGCCT 1 cut(s) 445
SspI AATATT 2 cut(s) 216, 510
SspMI CTAG 2 cut(s) 695, 1067
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 5 cut(s) 418, 484, 595, 802, 852
StyI CCWWGG 1 cut(s) 669
TaaI ACNGT 4 cut(s) 47, 59, 388, 1008
TaqI TCGA 2 cut(s) 117, 240
TasI AATT 5 cut(s) 220, 362, 532, 559, 628
TatI WGTACW 1 cut(s) 126
TfiI GAWTC 4 cut(s) 32, 109, 698, 1026
Tru1I TTAA 2 cut(s) 81, 249
Tru9I TTAA 2 cut(s) 81, 249
TscAI CASTG 2 cut(s) 786, 882
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 3 cut(s) 551, 734, 893
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 5 cut(s) 149, 329, 736, 915, 1026
TspGWI ACGGA 3 cut(s) 162, 343, 610
TspRI CASTG 2 cut(s) 786, 882
Van91I CCANNNNNTGG 1 cut(s) 209
Vha464I CTTAAG 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 1062
XceI RCATGY 2 cut(s) 127, 343
XmiI GTMKAC 1 cut(s) 497
XspI CTAG 2 cut(s) 695, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.