Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0000861

Physical Location & Seq

Genomic Coordinates Reverse (-)

13990693 .. 13993992

Gene Structure 3300 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g16040.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGAGAGATCAAGAGGATGTTGAAACAAATTCAAGGCAAGGTTTTGCTCAAGACTTTTCCAACATGGACAATGGAGTCCCACAACCAGTCACAATGAGTCCCAAAAACTGGGCAGGTGGTGAGCTCAATCCACAAGATGCTTGGCTACCCCTCACAGAGTCCAGAAAAGGGACTATCTATTCTACTACCTTTCATCTTCTGTGTTCAGGACTTGGAATTCAAGCCCTTTTACTACCTGTTGCTTTTGCTACTCTTGGATGGGCATGGGGAATCATATGCTTGTCAATAGTGTGTTCATGGAAGTTCTACACGATGTCACTCCTTGTACGATTACATGAATCTGATTCTGGAATTCGATATAGTAGATACGTCCACCTTGCAGTAACTGCCTTTGGTCCAAAGCTAGGGATGTTGTTAGCTTTGTTCCCAGCAATGTATCTTTCTGGTGGTGCGTGTGTGCAGCTAATCATCATAGGAGGTGGAATTATAAAGCTTCTAGTAAGAACAGTGTGCAAGGATGGTGCAACTTGTGATCCTAATTCAATGCATGGTATATTGTGCTTCTTTGTGTTTATGTTCATGGCTATCTGTGTAGCCGAGTACTTCCCCAACTTGAACTCCGTAGCTGGAGTCTCTGTGATTGGTACCATTACAGCAATTGTATATTGTACCATGATATGGGCTCTTTCAATCGGCAAGGGCAGGCTTAATGATGTATCGTATGAGCCTCCAGCTATGGACTCTACCATGGAGTCCTTCAGTAGTGTACTGAATTCAGTTGGCATCATTTTCCTTGCATTCAGAGGTCAAAATGTTATCCTTGAGATACAGGGGACATTGCCATCAAACCCAAGACATCCTTCATGTAAGAGAGCGTGGAGTGGAGTGACTATATCACATGTAGTAATAGCCTTGTGTTTGTTTTCTCTTGCACTTGGTGGATATCGGGCTTATGGAAACAAGGTACCTTTCGCGAATACAGAAGTGGTGAAAGCCGTTTCTCAGTTCCATGCACAAAAAATCTCACAATTTGTCCTGGAACTACTTTGCATACTGGTATTGATAAACTGCTTGACTACATTCCAAATCTATGGCATGGTAGTTTTTGACAACTTTGAGATGAAGTACACCATTAGGAAGAAGCAGCCGTGCCCGAGATGGCTTCGGATAGTTTTACGCCTTTTCGTTGGTGGATTTGCATTCTTTTTAGCAGTTGCCTTGCCATTCTTGGGAAGACTATCACCCCTACTTGGGGCAGTTACATCAGTGCCTTTGACATATGTTTATCCATGTTTTATGTGGAATTCTATCAAGAAACCAAAGCCAAAAGGCGTCATTTGGTGTACTAATATGGGACTCGGATGCTTGGGAGTGGTTTTGAGTGTTCTTTTTGTTGTTGCAGCAGCTTGGGATTTGGCTGACAAGGGCTTGGTTGCCAACTTCTTCAAGCCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0008150 GO:0008509 GO:0008514 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0022857 GO:0034220 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0098656 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Aa_trans

Protein Analysis

485

Amino Acids

53.14

Weight (kDa)

8.96

Isoelectric Point (pI)

32.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aa_trans	PF01490	56 - 466	5.2e-52	Transmembrane amino acid transporter protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000861)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35180 AT4G35180
fragaria_vesca	FvH4_2g16040 FvH4_2g16050 FvH4_2g16052 FvH4_2g16052 FvH4_2g16052 FvH4_2g16053 FvH4_2g16053
malus_domestica	MD05G1129500.v1.1 MD05G1129600.v1.1 MD05G1129800.v1.1 MD10G1132700.v1.1
prunus_persica	Prupe.8G173300_v2.0.a1 Prupe.8G173400_v2.0.a1 Prupe.8G173500_v2.0.a1
pyrus_communis	pycom05g12580 pycom05g12590 pycom10g11390 pycom10g11400
rosa_chinensis	RchiOBHm_Chr2g0160371 RchiOBHm_Chr6g0279621 RchiOBHm_Chr6g0279631 RchiOBHm_Chr6g0279671 RchiOBHm_Chr6g0279681 RchiOBHm_Chr6g0279691
rosa_laevigata	RLG00000013127 RLG00000013129 RLG00000013130 RLG00000013132
rosa_multiflora	Rmu_sc0000079.1_g000070 Rmu_sc0000685.1_g000010 Rmu_sc0001225.1_g000006 Rmu_sc0001719.1_g000006 Rmu_sc0003865.1_g000001 Rmu_sc0004010.1_g000009 Rmu_sc0004010.1_g000011
rosa_roxburghii	Rroxscaffold_7G00188730 Rroxscaffold_7G00188750 Rroxscaffold_7G00188760 Rroxscaffold_7G00188780
rosa_rugosa	Rorug06G0126500 Rorug06G0126900 Rorug06G0127000 Rorug06G0127100 Rorug06G0127200.1 Rorug06G0127300 Rorug06G0127700
rosa_samantha	Rh2BG398100 Rh6AG235000 Rh6AG235200 Rh6AG235400 Rh6BG239500 Rh6BG239600 Rh6BG239900 Rh6CG241300 Rh6CG241500 Rh6CG241600 Rh6CG241800 Rh6DG232800 Rh6DG233100 Rh6DG233200 Rh6DG233400
rosa_wichuraiana	Rw6G020510 Rw6G020520 Rw6G020530 Rw7G027130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	488
AarI	CACCTGC	1 cut(s)	104
AasI	GACNNNNNNGTC	1 cut(s)	74
Acc36I	ACCTGC	1 cut(s)	104
Acc65I	GGTACC	2 cut(s)	644, 964
AccB1I	GGYRCC	2 cut(s)	644, 964
AccB7I	CCANNNNNTGG	2 cut(s)	108, 678
AccII	CGCG	1 cut(s)	974
AclWI	GGATC	1 cut(s)	527
AcsI	RAATTY	5 cut(s)	28, 216, 351, 772, 1303
AcuI	CTGAAG	1 cut(s)	742
AcyI	GRCGYC	1 cut(s)	1332
AdeI	CACNNNGTG	1 cut(s)	938
AfaI	GTAC	8 cut(s)	327, 602, 646, 670, 768, 966, 1127, 1345
AfiI	CCNNNNNNNGG	8 cut(s)	108, 168, 404, 678, 1229, 1250, 1251, 1252
AflIII	ACRYGT	1 cut(s)	898
AgsI	TTSAA	7 cut(s)	23, 33, 221, 543, 616, 690, 1447
AjnI	CCWGG	1 cut(s)	1035
AjuI	GAANNNNNNNTTGG	2 cut(s)	844, 876
AluBI	AGCT	8 cut(s)	124, 403, 419, 463, 493, 626, 734, 1406
AluI	AGCT	8 cut(s)	124, 403, 419, 463, 493, 626, 734, 1406
Alw21I	GWGCWC	1 cut(s)	126
Alw26I	GTCTC	1 cut(s)	637
AlwI	GGATC	1 cut(s)	527
AlwNI	CAGNNNCTG	1 cut(s)	386
Ama87I	CYCGRG	1 cut(s)	1153
ApeKI	GCWGC	4 cut(s)	460, 1144, 1400, 1403
ApoI	RAATTY	5 cut(s)	28, 216, 351, 772, 1303
Asp718I	GGTACC	2 cut(s)	644, 964
AspS9I	GGNCC	1 cut(s)	395
AsuHPI	GGTGA	3 cut(s)	131, 1000, 1233
AvaI	CYCGRG	1 cut(s)	1153
AvaII	GGWCC	1 cut(s)	395
BaeGI	GKGCMC	1 cut(s)	1154
BanI	GGYRCC	2 cut(s)	644, 964
BanII	GRGCYC	2 cut(s)	126, 685
BbsI	GAAGAC	1 cut(s)	1240
Bbv12I	GWGCWC	1 cut(s)	126
BbvI	GCAGC	4 cut(s)	472, 1156, 1412, 1415
BccI	CCATC	4 cut(s)	252, 512, 850, 1152
BceAI	ACGGC	3 cut(s)	980, 1132, 1435
BciT130I	CCWGG	1 cut(s)	1037
BcoDI	GTCTC	1 cut(s)	637
BfaI	CTAG	2 cut(s)	404, 497
BfuAI	ACCTGC	1 cut(s)	104
BisI	GCNGC	4 cut(s)	461, 1145, 1401, 1404
BlsI	GCNGC	4 cut(s)	462, 1146, 1402, 1405
BmcAI	AGTACT	1 cut(s)	602
Bme1390I	CCNGG	1 cut(s)	1037
Bme18I	GGWCC	1 cut(s)	395
BmeT110I	CYCGRG	1 cut(s)	1153
BmgT120I	GGNCC	1 cut(s)	395
BmiI	GGNNCC	2 cut(s)	646, 966
BmrFI	CCNGG	1 cut(s)	1037
BmrI	ACTGGG	1 cut(s)	118
BmsI	GCATC	3 cut(s)	127, 792, 1352
BmuI	ACTGGG	1 cut(s)	118
BpiI	GAAGAC	1 cut(s)	1240
BpmI	CTGGAG	2 cut(s)	648, 714
BpuEI	CTTGAG	2 cut(s)	33, 842
BsaHI	GRCGYC	1 cut(s)	1332
BsaJI	CCNNGG	1 cut(s)	747
BsaXI	ACNNNNNCTCC	4 cut(s)	602, 621, 632, 651
Bsc4I	CCNNNNNNNGG	8 cut(s)	108, 168, 404, 678, 1229, 1250, 1251, 1252
Bse1I	ACTGG	3 cut(s)	86, 113, 1059
Bse3DI	GCAATG	2 cut(s)	438, 836
BseBI	CCWGG	1 cut(s)	1037
BseDI	CCNNGG	1 cut(s)	747
BseGI	GGATG	6 cut(s)	22, 263, 414, 523, 856, 1367
BseLI	CCNNNNNNNGG	8 cut(s)	108, 168, 404, 678, 1229, 1250, 1251, 1252
BseMI	GCAATG	2 cut(s)	438, 836
BseMII	CTCAG	1 cut(s)	1016
BseNI	ACTGG	3 cut(s)	86, 113, 1059
BseSI	GKGCMC	1 cut(s)	1154
BseXI	GCAGC	4 cut(s)	472, 1156, 1412, 1415
BseYI	CCCAGC	1 cut(s)	427
BsgI	GTGCAG	1 cut(s)	479
Bsh1236I	CGCG	1 cut(s)	974
BshNI	GGYRCC	2 cut(s)	644, 964
BsiHKAI	GWGCWC	1 cut(s)	126
BsiHKCI	CYCGRG	1 cut(s)	1153
BslFI	GGGAC	5 cut(s)	62, 84, 184, 847, 1368
BslI	CCNNNNNNNGG	8 cut(s)	108, 168, 404, 678, 1229, 1250, 1251, 1252
BsmAI	GTCTC	1 cut(s)	637
BsmFI	GGGAC	5 cut(s)	62, 84, 184, 847, 1368
BsmI	GAATGC	2 cut(s)	797, 1199
BsoBI	CYCGRG	1 cut(s)	1153
Bsp1286I	GDGCHC	3 cut(s)	126, 685, 1154
Bsp143I	GATC	2 cut(s)	7, 532
Bsp19I	CCATGG	1 cut(s)	747
Bsp68I	TCGCGA	1 cut(s)	974
BspCNI	CTCAG	1 cut(s)	1015
BspFNI	CGCG	1 cut(s)	974
BspLI	GGNNCC	2 cut(s)	646, 966
BspMI	ACCTGC	1 cut(s)	104
BspPI	GGATC	1 cut(s)	527
BspT107I	GGYRCC	2 cut(s)	644, 964
BsrDI	GCAATG	2 cut(s)	438, 836
BsrI	ACTGG	3 cut(s)	86, 113, 1059
BssECI	CCNNGG	1 cut(s)	747
BssMI	GATC	2 cut(s)	7, 532
BssNI	GRCGYC	1 cut(s)	1332
BssT1I	CCWWGG	1 cut(s)	747
Bst2UI	CCWGG	1 cut(s)	1037
Bst4CI	ACNGT	1 cut(s)	508
BstACI	GRCGYC	1 cut(s)	1332
BstAPI	GCANNNNNTGC	1 cut(s)	386
BstC8I	GCNNGC	1 cut(s)	704
BstDEI	CTNAG	1 cut(s)	1002
BstDSI	CCRYGG	1 cut(s)	747
BstF5I	GGATG	6 cut(s)	22, 263, 414, 523, 856, 1367
BstFNI	CGCG	1 cut(s)	974
BstKTI	GATC	2 cut(s)	10, 535
BstMAI	GTCTC	1 cut(s)	637
BstMBI	GATC	2 cut(s)	7, 532
BstMWI	GCNNNNNNNGC	1 cut(s)	386
BstNI	CCWGG	1 cut(s)	1037
BstNSI	RCATGY	1 cut(s)	902
BstSCI	CCNGG	1 cut(s)	1035
BstSLI	GKGCMC	1 cut(s)	1154
BstUI	CGCG	1 cut(s)	974
BstV1I	GCAGC	4 cut(s)	472, 1156, 1412, 1415
BstV2I	GAAGAC	1 cut(s)	1240
BstXI	CCANNNNNNTGG	1 cut(s)	1091
BtgI	CCRYGG	1 cut(s)	747
BtsCI	GGATG	6 cut(s)	22, 263, 414, 523, 856, 1367
BtsIMutI	CAGTG	2 cut(s)	513, 1272
BtuMI	TCGCGA	1 cut(s)	974
BveI	ACCTGC	1 cut(s)	104
Cac8I	GCNNGC	1 cut(s)	704
CaiI	CAGNNNCTG	1 cut(s)	386
Cfr13I	GGNCC	1 cut(s)	395
CseI	GACGC	1 cut(s)	1321
Csp6I	GTAC	8 cut(s)	326, 601, 645, 669, 767, 965, 1126, 1344
CviQI	GTAC	8 cut(s)	326, 601, 645, 669, 767, 965, 1126, 1344
DdeI	CTNAG	1 cut(s)	1002
DpnI	GATC	2 cut(s)	9, 534
DpnII	GATC	2 cut(s)	7, 532
DraIII	CACNNNGTG	1 cut(s)	938
DrdI	GACNNNNNNGTC	1 cut(s)	74
DseDI	GACNNNNNNGTC	1 cut(s)	74
Ecl136II	GAGCTC	1 cut(s)	124
Eco130I	CCWWGG	1 cut(s)	747
Eco24I	GRGCYC	2 cut(s)	126, 685
Eco32I	GATATC	1 cut(s)	944
Eco47I	GGWCC	1 cut(s)	395
Eco53kI	GAGCTC	1 cut(s)	124
Eco57I	CTGAAG	1 cut(s)	742
Eco88I	CYCGRG	1 cut(s)	1153
EcoICRI	GAGCTC	1 cut(s)	124
EcoRI	GAATTC	4 cut(s)	216, 351, 772, 1303
EcoRII	CCWGG	1 cut(s)	1035
EcoRV	GATATC	1 cut(s)	944
EcoT14I	CCWWGG	1 cut(s)	747
EcoT22I	ATGCAT	1 cut(s)	549
EcoT38I	GRGCYC	2 cut(s)	126, 685
ErhI	CCWWGG	1 cut(s)	747
FalI	AAGNNNNNCTT	2 cut(s)	844, 876
FaqI	GGGAC	5 cut(s)	62, 84, 184, 847, 1368
FauNDI	CATATG	2 cut(s)	275, 1279
Fnu4HI	GCNGC	4 cut(s)	461, 1145, 1401, 1404
FokI	GGATG	6 cut(s)	29, 270, 421, 530, 843, 1374
FriOI	GRGCYC	2 cut(s)	126, 685
Fsp4HI	GCNGC	4 cut(s)	461, 1145, 1401, 1404
FspBI	CTAG	2 cut(s)	404, 497
GluI	GCNGC	4 cut(s)	461, 1145, 1401, 1404
GsaI	CCCAGC	1 cut(s)	431
GsuI	CTGGAG	2 cut(s)	648, 714
HgaI	GACGC	1 cut(s)	1321
Hin1I	GRCGYC	1 cut(s)	1332
HindIII	AAGCTT	1 cut(s)	491
HphI	GGTGA	3 cut(s)	131, 1000, 1233
Hpy166II	GTNNAC	4 cut(s)	373, 767, 1128, 1344
Hpy188I	TCNGA	4 cut(s)	343, 803, 1167, 1361
Hpy188III	TCNNGA	7 cut(s)	11, 50, 162, 207, 348, 973, 1312
Hpy8I	GTNNAC	4 cut(s)	373, 767, 1128, 1344
HpyAV	CCTTC	2 cut(s)	766, 870
HpyCH4III	ACNGT	1 cut(s)	508
HpyCH4IV	ACGT	1 cut(s)	369
HpyF10VI	GCNNNNNNNGC	1 cut(s)	386
HpyF3I	CTNAG	1 cut(s)	1002
HpySE526I	ACGT	1 cut(s)	369
Hsp92I	GRCGYC	1 cut(s)	1332
KpnI	GGTACC	2 cut(s)	648, 968
Kzo9I	GATC	2 cut(s)	7, 532
Lsp1109I	GCAGC	4 cut(s)	472, 1156, 1412, 1415
LweI	GCATC	3 cut(s)	127, 792, 1352
MaeI	CTAG	2 cut(s)	404, 497
MaeII	ACGT	1 cut(s)	369
MaeIII	GTNAC	5 cut(s)	88, 315, 382, 886, 1258
MalI	GATC	2 cut(s)	9, 534
MboI	GATC	2 cut(s)	7, 532
MboII	GAAGA	4 cut(s)	188, 1150, 1245, 1435
MfeI	CAATTG	1 cut(s)	657
MhlI	GDGCHC	3 cut(s)	126, 685, 1154
MluCI	AATT	9 cut(s)	28, 216, 351, 483, 538, 657, 772, 1028, 1303
MlyI	GAGTC	7 cut(s)	84, 106, 167, 639, 734, 761, 1350
MmeI	TCCRAC	1 cut(s)	84
MnlI	CCTC	5 cut(s)	7, 161, 470, 738, 797
Mph1103I	ATGCAT	1 cut(s)	549
MseI	TTAA	1 cut(s)	708
MspR9I	CCNGG	1 cut(s)	1037
MunI	CAATTG	1 cut(s)	657
Mva1269I	GAATGC	2 cut(s)	797, 1199
MvaI	CCWGG	1 cut(s)	1037
MvnI	CGCG	1 cut(s)	974
MwoI	GCNNNNNNNGC	1 cut(s)	386
NcoI	CCATGG	1 cut(s)	747
NdeI	CATATG	2 cut(s)	275, 1279
NdeII	GATC	2 cut(s)	7, 532
NlaIV	GGNNCC	2 cut(s)	646, 966
NmeAIII	GCCGAG	1 cut(s)	622
NmuCI	GTSAC	3 cut(s)	88, 315, 886
NruI	TCGCGA	1 cut(s)	974
NsiI	ATGCAT	1 cut(s)	549
NspI	RCATGY	1 cut(s)	902
PaqCI	CACCTGC	1 cut(s)	104
PciI	ACATGT	1 cut(s)	898
PctI	GAATGC	2 cut(s)	797, 1199
PfeI	GAWTC	3 cut(s)	270, 338, 344
PflMI	CCANNNNNTGG	2 cut(s)	108, 678
PfoI	TCCNGGA	1 cut(s)	1035
PkrI	GCNGC	4 cut(s)	462, 1146, 1402, 1405
PleI	GAGTC	7 cut(s)	83, 105, 166, 638, 734, 760, 1350
PpsI	GAGTC	7 cut(s)	83, 105, 166, 638, 734, 760, 1350
PscI	ACATGT	1 cut(s)	898
PsiI	TTATAA	1 cut(s)	488
Psp124BI	GAGCTC	1 cut(s)	126
Psp6I	CCWGG	1 cut(s)	1035
PspFI	CCCAGC	1 cut(s)	427
PspGI	CCWGG	1 cut(s)	1035
PspN4I	GGNNCC	2 cut(s)	646, 966
PspPI	GGNCC	1 cut(s)	395
PstNI	CAGNNNCTG	1 cut(s)	386
RruI	TCGCGA	1 cut(s)	974
RsaI	GTAC	8 cut(s)	327, 602, 646, 670, 768, 966, 1127, 1345
RsaNI	GTAC	8 cut(s)	326, 601, 645, 669, 767, 965, 1126, 1344
SacI	GAGCTC	1 cut(s)	126
SaqAI	TTAA	1 cut(s)	708
SatI	GCNGC	4 cut(s)	461, 1145, 1401, 1404
Sau3AI	GATC	2 cut(s)	7, 532
Sau96I	GGNCC	1 cut(s)	395
ScaI	AGTACT	1 cut(s)	602
SchI	GAGTC	7 cut(s)	84, 106, 167, 639, 734, 761, 1350
ScrFI	CCNGG	1 cut(s)	1037
SduI	GDGCHC	3 cut(s)	126, 685, 1154
SfaNI	GCATC	3 cut(s)	127, 792, 1352
SinI	GGWCC	1 cut(s)	395
SmlI	CTYRAG	2 cut(s)	48, 821
SmoI	CTYRAG	2 cut(s)	48, 821
Sse9I	AATT	9 cut(s)	28, 216, 351, 483, 538, 657, 772, 1028, 1303
SspMI	CTAG	2 cut(s)	404, 497
SstI	GAGCTC	1 cut(s)	126
StyD4I	CCNGG	1 cut(s)	1035
StyI	CCWWGG	1 cut(s)	747
TaaI	ACNGT	1 cut(s)	508
TaiI	ACGT	1 cut(s)	372
TaqI	TCGA	1 cut(s)	355
TasI	AATT	9 cut(s)	28, 216, 351, 483, 538, 657, 772, 1028, 1303
TatI	WGTACW	4 cut(s)	600, 766, 1125, 1343
TfiI	GAWTC	3 cut(s)	270, 338, 344
Tru1I	TTAA	1 cut(s)	708
Tru9I	TTAA	1 cut(s)	708
TscAI	CASTG	2 cut(s)	513, 1272
TseFI	GTSAC	3 cut(s)	88, 315, 886
TseI	GCWGC	4 cut(s)	460, 1144, 1400, 1403
Tsp45I	GTSAC	3 cut(s)	88, 315, 886
TspDTI	ATGAA	6 cut(s)	182, 285, 351, 568, 852, 1136
TspGWI	ACGGA	1 cut(s)	610
TspRI	CASTG	2 cut(s)	513, 1272
Van91I	CCANNNNNTGG	2 cut(s)	108, 678
VpaK11BI	GGWCC	1 cut(s)	395
XapI	RAATTY	5 cut(s)	28, 216, 351, 772, 1303
XceI	RCATGY	1 cut(s)	902
XcmI	CCANNNNNNNNNTGG	2 cut(s)	138, 1296
XspI	CTAG	2 cut(s)	404, 497
ZrmI	AGTACT	1 cut(s)	602
Zsp2I	ATGCAT	1 cut(s)	549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.