FvH4_2g21770

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002399
Physical Location & Seq
Reverse (-)
18015503 .. 18017278
1776 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21770.t1

Sequence Viewer

Length: 1179 bp
ATGGCTCTTGTAGTAAGTCCATACATGAAGATCTATAGGAAATTCAAGCCACATCTTCTCCTGGTTATGGCTCAGATCGGTTATACATTTCTCTACTTTATTACTGATGCATCCTTCAATCATGGGATGAACCCTCATGTCTTTGTAACCTATCGACATATAGTTGGTGGCTTAGTCATGTTTCCATTTGCCTATTTTCTTGAAAGGCATACAAGTCTCTCTTACAGTACTGCTTCTCTGCAGCAAAGCAAGGCCAAAGTTGACTCTAGCATTGTTTATGGAGATATTTGTGCTTGCACTGCTAGGGTGAAGCATCAAAATCATGTACACCGGTTAGATAAGTCTAAATCTTTCATGCTTCGTCTTGTTTTTACAAACATGTTTTTGATACTCACTTCCCATAGGTTGGAGGCTGTAGATGTGAGGAATCCACGCGGAATAGCTAAACTTTTGGGAACCTTAATATCCCTGGCTGGGGTTATTACCATGACTTCTTACAATGGACCTGCCATACAAAGCTTGTCAGGAGCATCCAAACACATAAGAAGTAACATTGTTCACAAAAGCTGGACAAAGGGTTCAATCCTTGTGATTACAAGTTGTATAACATGGTCCATATGGTACATAATGCAGGGGATGACACTGAAGAAATATCCTGCACAACTTTCACTAACCACATGGATCAATTGTATCGGCGCAGCGCAGTCAGCTGTGTTCACAGTTATCATACATCCCAAACAGGCAGCATGGAGAATTACATACAACAATGATTTCTGGTCCATCATATATGCTGGAGTTGTGTGCTCAGGTATAACAACCTTCATTCAACTGTGGTGTACAAAACAAAAAGGACCTGTTTTCGTGACCATGTTCAGTCCTCTTGCAACAGTATTAGTTGCGATTCTAGCTTACTTCATTCTCGGCGAAAAACTTCACGTTGGCAGAATAATGGGAGCGATCATTATCATTATCGGTCTATACCTAGTCTTATGGGGCAAAGATAGAGATAAAAATCATTTAAAGACCCAAGAGCAACCTACTCTGATTACTGATAACCAGAAGGAACCCAAGATGCATGCACAAGTGGGATCTTCAGCGGAAAGCCAGGTCCTCCAAATGGACCTTGAGACAGTTGAGAGTAAAGATGGAACCAATGGTTTAAGATTATGCATCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

393

Amino Acids

44.0

Weight (kDa)

9.66

Isoelectric Point (pI)

32.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 192 - 330 3.4e-16 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 514
AccB7I CCANNNNNTGG 1 cut(s) 406
AccII CGCG 1 cut(s) 435
AciI CCGC 2 cut(s) 435, 1097
AclWI GGATC 2 cut(s) 689, 1096
AcsI RAATTY 1 cut(s) 41
AcuI CTGAAG 2 cut(s) 665, 1077
AfaI GTAC 4 cut(s) 229, 327, 623, 838
AfiI CCNNNNNNNGG 5 cut(s) 67, 406, 474, 475, 1117
AflIII ACRYGT 1 cut(s) 378
AgeI ACCGGT 1 cut(s) 330
AgsI TTSAA 5 cut(s) 46, 118, 203, 582, 827
AjnI CCWGG 3 cut(s) 60, 468, 1104
AloI GAACNNNNNNTCC 2 cut(s) 562, 594
AluBI AGCT 5 cut(s) 443, 519, 567, 710, 908
AluI AGCT 5 cut(s) 443, 519, 567, 710, 908
Alw21I GWGCWC 1 cut(s) 806
Alw26I GTCTC 2 cut(s) 221, 1121
AlwI GGATC 2 cut(s) 689, 1096
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 3 cut(s) 241, 698, 743
ApoI RAATTY 1 cut(s) 41
AsiGI ACCGGT 1 cut(s) 330
Asp700I GAANNNNTTC 1 cut(s) 930
AspLEI GCGC 2 cut(s) 698, 703
AspS9I GGNCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
Bbv12I GWGCWC 1 cut(s) 806
BbvI GCAGC 3 cut(s) 253, 710, 755
BccI CCATC 2 cut(s) 788, 1139
BciT130I CCWGG 3 cut(s) 62, 470, 1106
BcoDI GTCTC 2 cut(s) 221, 1121
BfaI CTAG 4 cut(s) 267, 303, 905, 983
BfmI CTRYAG 3 cut(s) 34, 239, 414
BfuAI ACCTGC 1 cut(s) 514
BglII AGATCT 1 cut(s) 30
BisI GCNGC 3 cut(s) 242, 699, 744
BlsI GCNGC 3 cut(s) 243, 700, 745
BmcAI AGTACT 1 cut(s) 229
Bme1390I CCNGG 3 cut(s) 62, 470, 1106
Bme18I GGWCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
BmgT120I GGNCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
BmiI GGNNCC 3 cut(s) 457, 1065, 1150
BmrFI CCNGG 3 cut(s) 62, 470, 1106
BmsI GCATC 5 cut(s) 97, 119, 322, 539, 1062
BpmI CTGGAG 1 cut(s) 813
Bpu10I CCTNAGC 1 cut(s) 805
BpuEI CTTGAG 1 cut(s) 1145
BsaBI GATNNNNATC 2 cut(s) 962, 1011
BsaJI CCNNGG 1 cut(s) 468
BsaWI WCCGGW 1 cut(s) 330
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 5 cut(s) 67, 406, 474, 475, 1117
Bse118I RCCGGY 1 cut(s) 330
Bse8I GATNNNNATC 2 cut(s) 962, 1011
BseBI CCWGG 3 cut(s) 62, 470, 1106
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 5 cut(s) 110, 132, 530, 642, 730
BseJI GATNNNNATC 2 cut(s) 962, 1011
BseLI CCNNNNNNNGG 5 cut(s) 67, 406, 474, 475, 1117
BseMII CTCAG 2 cut(s) 86, 819
BseXI GCAGC 3 cut(s) 253, 710, 755
BseYI CCCAGC 1 cut(s) 473
BsgI GTGCAG 1 cut(s) 642
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 1 cut(s) 254
BshTI ACCGGT 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 806
BsiSI CCGG 1 cut(s) 331
BslI CCNNNNNNNGG 5 cut(s) 67, 406, 474, 475, 1117
BsmAI GTCTC 2 cut(s) 221, 1121
BsnI GGCC 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 806
Bsp1407I TGTACA 2 cut(s) 325, 836
Bsp143I GATC 5 cut(s) 30, 75, 681, 957, 1088
BspACI CCGC 2 cut(s) 435, 1097
BspANI GGCC 1 cut(s) 254
BspCNI CTCAG 2 cut(s) 85, 818
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 457, 1065, 1150
BspMAI CTGCAG 1 cut(s) 243
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 2 cut(s) 689, 1096
BsrFI RCCGGY 1 cut(s) 330
BsrGI TGTACA 2 cut(s) 325, 836
BssAI RCCGGY 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 5 cut(s) 30, 75, 681, 957, 1088
Bst2UI CCWGG 3 cut(s) 62, 470, 1106
Bst4CI ACNGT 5 cut(s) 227, 721, 831, 889, 1132
BstAUI TGTACA 2 cut(s) 325, 836
BstC8I GCNNGC 2 cut(s) 295, 1077
BstDEI CTNAG 3 cut(s) 72, 172, 805
BstF5I GGATG 5 cut(s) 110, 132, 530, 642, 730
BstFNI CGCG 1 cut(s) 435
BstHHI GCGC 2 cut(s) 698, 703
BstKTI GATC 5 cut(s) 33, 78, 684, 960, 1091
BstMAI GTCTC 2 cut(s) 221, 1121
BstMBI GATC 5 cut(s) 30, 75, 681, 957, 1088
BstMWI GCNNNNNNNGC 3 cut(s) 299, 707, 905
BstNI CCWGG 3 cut(s) 62, 470, 1106
BstNSI RCATGY 2 cut(s) 382, 1079
BstSCI CCNGG 3 cut(s) 60, 468, 1104
BstSFI CTRYAG 3 cut(s) 34, 239, 414
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 3 cut(s) 253, 710, 755
BstX2I RGATCY 2 cut(s) 30, 1088
BstYI RGATCY 2 cut(s) 30, 1088
BsuRI GGCC 1 cut(s) 254
BtsCI GGATG 5 cut(s) 110, 132, 530, 642, 730
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 297, 641
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 2 cut(s) 295, 1077
CfoI GCGC 2 cut(s) 698, 703
Cfr10I RCCGGY 1 cut(s) 330
Cfr13I GGNCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
Csp6I GTAC 4 cut(s) 228, 326, 622, 837
CspAI ACCGGT 1 cut(s) 330
CviQI GTAC 4 cut(s) 228, 326, 622, 837
DdeI CTNAG 3 cut(s) 72, 172, 805
DpnI GATC 5 cut(s) 32, 77, 683, 959, 1090
DpnII GATC 5 cut(s) 30, 75, 681, 957, 1088
DraI TTTAAA 1 cut(s) 1020
Eco47I GGWCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
Eco57I CTGAAG 2 cut(s) 665, 1077
EcoO109I RGGNCCY 2 cut(s) 851, 1108
EcoRII CCWGG 3 cut(s) 60, 468, 1104
EcoT22I ATGCAT 3 cut(s) 112, 1077, 1172
FalI AAGNNNNNCTT 2 cut(s) 205, 237
FauNDI CATATG 2 cut(s) 617, 1175
Fnu4HI GCNGC 3 cut(s) 242, 699, 744
FokI GGATG 5 cut(s) 97, 139, 517, 649, 717
Fsp4HI GCNGC 3 cut(s) 242, 699, 744
FspBI CTAG 4 cut(s) 267, 303, 905, 983
GlaI GCGC 2 cut(s) 697, 702
GluI GCNGC 3 cut(s) 242, 699, 744
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 1 cut(s) 813
HaeIII GGCC 1 cut(s) 254
HapII CCGG 1 cut(s) 331
HhaI GCGC 2 cut(s) 698, 703
Hin6I GCGC 2 cut(s) 696, 701
HinP1I GCGC 2 cut(s) 696, 701
HincII GTYRAC 1 cut(s) 262
HindII GTYRAC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 517
HinfI GANTC 3 cut(s) 263, 427, 901
HpaII CCGG 1 cut(s) 331
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 5 cut(s) 262, 328, 559, 717, 837
Hpy188I TCNGA 2 cut(s) 75, 1044
Hpy188III TCNNGA 3 cut(s) 200, 525, 862
Hpy8I GTNNAC 5 cut(s) 262, 328, 559, 717, 837
HpyAV CCTTC 3 cut(s) 124, 829, 1054
HpyCH4III ACNGT 5 cut(s) 227, 721, 831, 889, 1132
HpyCH4IV ACGT 1 cut(s) 936
HpyCH4V TGCA 9 cut(s) 110, 241, 297, 631, 659, 884, 1075, 1079, 1170
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 707, 905
HpyF3I CTNAG 3 cut(s) 72, 172, 805
HpySE526I ACGT 1 cut(s) 936
HspAI GCGC 2 cut(s) 696, 701
Kzo9I GATC 5 cut(s) 30, 75, 681, 957, 1088
LmnI GCTCC 2 cut(s) 527, 953
Lsp1109I GCAGC 3 cut(s) 253, 710, 755
LweI GCATC 5 cut(s) 97, 119, 322, 539, 1062
MaeI CTAG 4 cut(s) 267, 303, 905, 983
MaeII ACGT 1 cut(s) 936
MaeIII GTNAC 3 cut(s) 145, 548, 862
MalI GATC 5 cut(s) 32, 77, 683, 959, 1090
MboI GATC 5 cut(s) 30, 75, 681, 957, 1088
MboII GAAGA 4 cut(s) 40, 47, 658, 1083
MfeI CAATTG 1 cut(s) 685
MflI RGATCY 2 cut(s) 30, 1088
MhlI GDGCHC 1 cut(s) 806
MluCI AATT 3 cut(s) 41, 685, 753
MlyI GAGTC 1 cut(s) 257
MmeI TCCRAC 1 cut(s) 387
MnlI CCTC 5 cut(s) 144, 403, 417, 888, 1121
Mph1103I ATGCAT 3 cut(s) 112, 1077, 1172
MroXI GAANNNNTTC 1 cut(s) 930
MseI TTAA 3 cut(s) 461, 1019, 1160
MspA1I CMGCKG 2 cut(s) 710, 1097
MspI CCGG 1 cut(s) 331
MspR9I CCNGG 3 cut(s) 62, 470, 1106
MteI GCGCNGCGC 1 cut(s) 699
MunI CAATTG 1 cut(s) 685
MvaI CCWGG 3 cut(s) 62, 470, 1106
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 3 cut(s) 299, 707, 905
NdeI CATATG 2 cut(s) 617, 1175
NdeII GATC 5 cut(s) 30, 75, 681, 957, 1088
NlaIV GGNNCC 3 cut(s) 457, 1065, 1150
NmeAIII GCCGAG 1 cut(s) 900
NmuCI GTSAC 1 cut(s) 862
NsiI ATGCAT 3 cut(s) 112, 1077, 1172
NspI RCATGY 2 cut(s) 382, 1079
PaeI GCATGC 1 cut(s) 1079
PciI ACATGT 1 cut(s) 378
PdmI GAANNNNTTC 1 cut(s) 930
PfeI GAWTC 2 cut(s) 427, 901
PflMI CCANNNNNTGG 1 cut(s) 406
PinAI ACCGGT 1 cut(s) 330
PkrI GCNGC 3 cut(s) 243, 700, 745
PleI GAGTC 1 cut(s) 257
PpsI GAGTC 1 cut(s) 257
PpuMI RGGWCCY 2 cut(s) 851, 1108
PscI ACATGT 1 cut(s) 378
Psp5II RGGWCCY 2 cut(s) 851, 1108
Psp6I CCWGG 3 cut(s) 60, 468, 1104
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 3 cut(s) 60, 468, 1104
PspN4I GGNNCC 3 cut(s) 457, 1065, 1150
PspPI GGNCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
PspPPI RGGWCCY 2 cut(s) 851, 1108
PstI CTGCAG 1 cut(s) 243
PsuI RGATCY 2 cut(s) 30, 1088
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 4 cut(s) 229, 327, 623, 838
RsaNI GTAC 4 cut(s) 228, 326, 622, 837
SaqAI TTAA 3 cut(s) 461, 1019, 1160
SatI GCNGC 3 cut(s) 242, 699, 744
Sau3AI GATC 5 cut(s) 30, 75, 681, 957, 1088
Sau96I GGNCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
ScaI AGTACT 1 cut(s) 229
SchI GAGTC 1 cut(s) 257
ScrFI CCNGG 3 cut(s) 62, 470, 1106
SduI GDGCHC 1 cut(s) 806
SfaNI GCATC 5 cut(s) 97, 119, 322, 539, 1062
SfcI CTRYAG 3 cut(s) 34, 239, 414
SinI GGWCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
SmlI CTYRAG 1 cut(s) 1124
SmoI CTYRAG 1 cut(s) 1124
SphI GCATGC 1 cut(s) 1079
Sse9I AATT 3 cut(s) 41, 685, 753
SsiI CCGC 2 cut(s) 435, 1097
SspMI CTAG 4 cut(s) 267, 303, 905, 983
StyD4I CCNGG 3 cut(s) 60, 468, 1104
TaaI ACNGT 5 cut(s) 227, 721, 831, 889, 1132
TaiI ACGT 1 cut(s) 939
TaqI TCGA 1 cut(s) 154
TaqII GACCGA 1 cut(s) 962
TasI AATT 3 cut(s) 41, 685, 753
TatI WGTACW 3 cut(s) 227, 325, 836
TfiI GAWTC 2 cut(s) 427, 901
Tru1I TTAA 3 cut(s) 461, 1019, 1160
Tru9I TTAA 3 cut(s) 461, 1019, 1160
TscAI CASTG 2 cut(s) 304, 648
TseFI GTSAC 1 cut(s) 862
TseI GCWGC 3 cut(s) 241, 698, 743
Tsp45I GTSAC 1 cut(s) 862
TspDTI ATGAA 5 cut(s) 41, 143, 343, 811, 904
TspRI CASTG 2 cut(s) 304, 648
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 6 cut(s) 503, 612, 777, 851, 1108, 1120
XapI RAATTY 1 cut(s) 41
XceI RCATGY 2 cut(s) 382, 1079
XmnI GAANNNNTTC 1 cut(s) 930
XspI CTAG 4 cut(s) 267, 303, 905, 983
ZrmI AGTACT 1 cut(s) 229
Zsp2I ATGCAT 3 cut(s) 112, 1077, 1172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.