Rroxscaffold_5G00337550

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0002399
Physical Location & Seq
Forward (+)
5733505 .. 5739872
6368 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00337550.1

Sequence Viewer

Length: 1308 bp
ATGGCACAAAAGGCAAGTTCACATGTGGGAGTTTGCAGGAAGTTCAAGCCTCACTTCCTCATGGTTTTAGCTCAAATGGGCTACACATTTCTTTATTTCATCACAGAAGCTTCCTTCAATCATGGGATGAACCCTCATGTCTACATTACTTATCGGCATATTGTGTCCGGCGTAGTGATGCTCCCTTTTGCATATTTTCTTGAAAGGTTGGAGGCTCTTAATCTTCGAAATCCTCGTGGGTTAGCAAAAGTTTTGGGCACCCTAGTCTCTTTAGCAGGAGTAATGACCATGACTTTGTACAAGGGACCAACTGTGAGAAATCTATGGAGTCCACCAATCCATATTGAAGGAAAATCCTCCATCCATGAGAACTGGTTGAAGGGTTCAATTCTCACTGTTGCTAGCTGCATAACTTGGTCTGTCTGGTACATTATGCAGGCAATCACGTTGAAAAGATATCCTGCACAACTGTCACTGACTACATGGATGAGCTTCATAGGGGCAGCACAGTCAGCTGTGTTCACCGTGTGTATTGAACATAGACGAGCTGCTTGGACCGTTGGATTCAACATTGATCTCTGGTCCATTCTGTACGCCGGAGTGGTGTGCTCTGGTCTAGTAATCTTCATTCAACTATGGTGCACAGAAGAAAAAGGACCAGTCTTTGTGACCATGTTCAATCCTGTTTCAACAATCTTGGTGGCAATTCTAGCATACTTTGTGCTCGGTGAAAGACTTTACATCGGCAGCATATTAGGGGCAGCTATTGTCATTGTTGGCCTATACTTGCTGTTGTGGGGGAAAGAAGGTGATGAAGTTTACATCATTAAGTCTGAAGACTCATCTGATCAGACATACGAAGAGGAGAAAGATGACAGAATACGAACAATCACTTCAGTCAAGAAGGATGCATTACATGGCAATCACATTAAAAAGATATCCTGCAAAACTATCACTGACAACATGGATGAGCTTCCTAGGGGCAGCACGGTCAGCATCTTCGCATTTTTTATAGAACATAAACAAGCTGCTTGGACAATTGGATTCAACATTGACCTCTGGTCCATTTTGTACTCTGTCAAGTTTCAGGATTCTGAAAAAAAAAATTTGTTTGTGAGTGCATTATTCTTTGTTAATAGCCAATGGTTAGATTGCGTATTTAATTATCTGCAGGGAGGGGTGTGCTCTGGCCTAATTCTCTTTATTCAACTATGGTGCACATTAGAGAAAGGACCTGTCTTTGTGACCATGTTTGATCCCATTTCGACAATATTGGTGGCAATTCGAAAGACAGACATTGGCAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

435

Amino Acids

49.12

Weight (kDa)

8.6

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 127 - 265 4.5e-15 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1293
AccB1I GGYRCC 1 cut(s) 257
AccI GTMKAC 1 cut(s) 141
AclWI GGATC 1 cut(s) 1250
AcsI RAATTY 1 cut(s) 1105
AcuI CTGAAG 2 cut(s) 855, 879
AdeI CACNNNGTG 1 cut(s) 528
AfaI GTAC 4 cut(s) 299, 428, 593, 1073
AflIII ACRYGT 1 cut(s) 22
AhdI GACNNNNNGTC 1 cut(s) 1060
AluBI AGCT 9 cut(s) 71, 110, 405, 492, 515, 548, 764, 973, 1028
AluI AGCT 9 cut(s) 71, 110, 405, 492, 515, 548, 764, 973, 1028
Alw21I GWGCWC 5 cut(s) 611, 644, 726, 1187, 1220
Alw26I GTCTC 1 cut(s) 271
Alw44I GTGCAC 2 cut(s) 640, 1216
AlwI GGATC 1 cut(s) 1250
AoxI GGCC 2 cut(s) 778, 1189
ApaLI GTGCAC 2 cut(s) 640, 1216
ApeKI GCWGC 7 cut(s) 405, 503, 548, 747, 761, 984, 1028
ApoI RAATTY 1 cut(s) 1105
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AspA2I CCTAGG 1 cut(s) 977
AspS9I GGNCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
AsuHPI GGTGA 3 cut(s) 514, 740, 821
AsuII TTCGAA 2 cut(s) 226, 1285
AsuNHI GCTAGC 1 cut(s) 401
AvaII GGWCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
AvrII CCTAGG 1 cut(s) 977
BaeGI GKGCMC 3 cut(s) 260, 644, 1220
BanI GGYRCC 1 cut(s) 257
BauI CACGAG 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 5 cut(s) 611, 644, 726, 1187, 1220
BbvI GCAGC 7 cut(s) 392, 515, 535, 759, 773, 996, 1015
BccI CCATC 1 cut(s) 368
BclI TGATCA 1 cut(s) 847
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 5 cut(s) 263, 402, 617, 710, 978
BfmI CTRYAG 1 cut(s) 1169
BfuAI ACCTGC 1 cut(s) 1293
BisI GCNGC 7 cut(s) 406, 504, 549, 748, 762, 985, 1029
BlnI CCTAGG 1 cut(s) 977
BlsI GCNGC 7 cut(s) 407, 505, 550, 749, 763, 986, 1030
Bme18I GGWCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
BmeRI GACNNNNNGTC 1 cut(s) 1060
BmgT120I GGNCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
BmiI GGNNCC 2 cut(s) 259, 306
BmsI GCATC 3 cut(s) 168, 898, 1005
BmtI GCTAGC 1 cut(s) 405
BpiI GAAGAC 1 cut(s) 843
Bpu14I TTCGAA 2 cut(s) 226, 1285
BsaJI CCNNGG 1 cut(s) 977
Bse1I ACTGG 2 cut(s) 377, 659
BseDI CCNNGG 1 cut(s) 977
BseGI GGATG 5 cut(s) 132, 360, 492, 913, 973
BseNI ACTGG 2 cut(s) 377, 659
BseRI GAGGAG 1 cut(s) 878
BseSI GKGCMC 3 cut(s) 260, 644, 1220
BseXI GCAGC 7 cut(s) 392, 515, 535, 759, 773, 996, 1015
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 2 cut(s) 780, 1191
BshNI GGYRCC 1 cut(s) 257
BsiHKAI GWGCWC 5 cut(s) 611, 644, 726, 1187, 1220
BsiSI CCGG 2 cut(s) 168, 597
BslFI GGGAC 1 cut(s) 318
BsmAI GTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 318
BsnI GGCC 2 cut(s) 780, 1191
Bsp119I TTCGAA 2 cut(s) 226, 1285
Bsp1286I GDGCHC 6 cut(s) 260, 611, 644, 726, 1187, 1220
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 3 cut(s) 574, 847, 1255
BspANI GGCC 2 cut(s) 780, 1191
BspLI GGNNCC 2 cut(s) 259, 306
BspMAI CTGCAG 1 cut(s) 1173
BspMI ACCTGC 1 cut(s) 1293
BspOI GCTAGC 1 cut(s) 405
BspPI GGATC 1 cut(s) 1250
BspT104I TTCGAA 2 cut(s) 226, 1285
BspT107I GGYRCC 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 2 cut(s) 377, 659
BssECI CCNNGG 1 cut(s) 977
BssMI GATC 3 cut(s) 574, 847, 1255
BssSI CACGAG 1 cut(s) 234
BssT1I CCWWGG 1 cut(s) 977
Bst2BI CACGAG 1 cut(s) 234
Bst4CI ACNGT 7 cut(s) 313, 397, 471, 510, 526, 559, 991
Bst6I CTCTTC 1 cut(s) 855
BstAUI TGTACA 1 cut(s) 297
BstBI TTCGAA 2 cut(s) 226, 1285
BstC8I GCNNGC 2 cut(s) 403, 438
BstF5I GGATG 5 cut(s) 132, 360, 492, 913, 973
BstKTI GATC 3 cut(s) 577, 850, 1258
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 3 cut(s) 574, 847, 1255
BstMWI GCNNNNNNNGC 4 cut(s) 11, 512, 710, 993
BstNSI RCATGY 1 cut(s) 26
BstSFI CTRYAG 1 cut(s) 1169
BstSLI GKGCMC 3 cut(s) 260, 644, 1220
BstV1I GCAGC 7 cut(s) 392, 515, 535, 759, 773, 996, 1015
BstV2I GAAGAC 1 cut(s) 843
BsuRI GGCC 2 cut(s) 780, 1191
BtsCI GGATG 5 cut(s) 132, 360, 492, 913, 973
BtsIMutI CAGTG 3 cut(s) 393, 473, 954
BveI ACCTGC 1 cut(s) 1293
Cac8I GCNNGC 2 cut(s) 403, 438
Cfr13I GGNCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
Csp6I GTAC 4 cut(s) 298, 427, 592, 1072
CspCI CAANNNNNGTGG 4 cut(s) 681, 716, 1257, 1292
CviQI GTAC 4 cut(s) 298, 427, 592, 1072
DpnI GATC 3 cut(s) 576, 849, 1257
DpnII GATC 3 cut(s) 574, 847, 1255
DraIII CACNNNGTG 1 cut(s) 528
DriI GACNNNNNGTC 1 cut(s) 1060
Eam1104I CTCTTC 1 cut(s) 855
Eam1105I GACNNNNNGTC 1 cut(s) 1060
EarI CTCTTC 1 cut(s) 855
Eco130I CCWWGG 1 cut(s) 977
Eco32I GATATC 2 cut(s) 458, 939
Eco47I GGWCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
Eco57I CTGAAG 2 cut(s) 855, 879
EcoO109I RGGNCCY 1 cut(s) 1232
EcoRV GATATC 2 cut(s) 458, 939
EcoT14I CCWWGG 1 cut(s) 977
EcoT22I ATGCAT 1 cut(s) 913
ErhI CCWWGG 1 cut(s) 977
FalI AAGNNNNNCTT 2 cut(s) 38, 70
FaqI GGGAC 1 cut(s) 318
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 7 cut(s) 406, 504, 549, 748, 762, 985, 1029
FokI GGATG 5 cut(s) 139, 347, 499, 920, 980
Fsp4HI GCNGC 7 cut(s) 406, 504, 549, 748, 762, 985, 1029
FspBI CTAG 5 cut(s) 263, 402, 617, 710, 978
GluI GCNGC 7 cut(s) 406, 504, 549, 748, 762, 985, 1029
HaeIII GGCC 2 cut(s) 780, 1191
HapII CCGG 2 cut(s) 168, 597
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 5 cut(s) 328, 564, 839, 1044, 1091
HpaII CCGG 2 cut(s) 168, 597
HphI GGTGA 3 cut(s) 514, 740, 821
Hpy166II GTNNAC 7 cut(s) 20, 142, 332, 522, 642, 820, 1218
Hpy188I TCNGA 4 cut(s) 835, 847, 852, 1096
Hpy188III TCNNGA 3 cut(s) 200, 901, 1088
Hpy8I GTNNAC 7 cut(s) 20, 142, 332, 522, 642, 820, 1218
HpyAV CCTTC 5 cut(s) 124, 341, 373, 800, 898
HpyCH4III ACNGT 7 cut(s) 313, 397, 471, 510, 526, 559, 991
HpyCH4IV ACGT 1 cut(s) 446
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 512, 710, 993
HpySE526I ACGT 1 cut(s) 446
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 3 cut(s) 574, 847, 1255
LmnI GCTCC 1 cut(s) 186
Lsp1109I GCAGC 7 cut(s) 392, 515, 535, 759, 773, 996, 1015
LweI GCATC 3 cut(s) 168, 898, 1005
MaeI CTAG 5 cut(s) 263, 402, 617, 710, 978
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 3 cut(s) 471, 667, 1243
MalI GATC 3 cut(s) 576, 849, 1257
MboI GATC 3 cut(s) 574, 847, 1255
MboII GAAGA 6 cut(s) 215, 616, 659, 848, 872, 991
MfeI CAATTG 1 cut(s) 1038
MhlI GDGCHC 6 cut(s) 260, 611, 644, 726, 1187, 1220
MluCI AATT 7 cut(s) 387, 705, 1038, 1105, 1162, 1194, 1281
MlyI GAGTC 2 cut(s) 337, 833
MmeI TCCRAC 2 cut(s) 189, 541
MnlI CCTC 9 cut(s) 60, 68, 144, 205, 243, 367, 856, 1067, 1169
Mph1103I ATGCAT 1 cut(s) 913
MseI TTAA 5 cut(s) 219, 828, 930, 1134, 1161
MspA1I CMGCKG 1 cut(s) 515
MspI CCGG 2 cut(s) 168, 597
MunI CAATTG 1 cut(s) 1038
MwoI GCNNNNNNNGC 4 cut(s) 11, 512, 710, 993
NdeII GATC 3 cut(s) 574, 847, 1255
NheI GCTAGC 1 cut(s) 401
NlaIV GGNNCC 2 cut(s) 259, 306
NmuCI GTSAC 3 cut(s) 471, 667, 1243
NsiI ATGCAT 1 cut(s) 913
NspI RCATGY 1 cut(s) 26
NspV TTCGAA 2 cut(s) 226, 1285
PciI ACATGT 1 cut(s) 22
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PfeI GAWTC 3 cut(s) 564, 1044, 1091
PkrI GCNGC 7 cut(s) 407, 505, 550, 749, 763, 986, 1030
PleI GAGTC 2 cut(s) 336, 833
PpsI GAGTC 2 cut(s) 336, 833
PpuMI RGGWCCY 1 cut(s) 1232
PscI ACATGT 1 cut(s) 22
Psp5II RGGWCCY 1 cut(s) 1232
PspN4I GGNNCC 2 cut(s) 259, 306
PspPI GGNCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
PspPPI RGGWCCY 1 cut(s) 1232
PstI CTGCAG 1 cut(s) 1173
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 4 cut(s) 299, 428, 593, 1073
RsaNI GTAC 4 cut(s) 298, 427, 592, 1072
SaqAI TTAA 5 cut(s) 219, 828, 930, 1134, 1161
SatI GCNGC 7 cut(s) 406, 504, 549, 748, 762, 985, 1029
Sau3AI GATC 3 cut(s) 574, 847, 1255
Sau96I GGNCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
SchI GAGTC 2 cut(s) 337, 833
SduI GDGCHC 6 cut(s) 260, 611, 644, 726, 1187, 1220
SfaNI GCATC 3 cut(s) 168, 898, 1005
SfcI CTRYAG 1 cut(s) 1169
SfuI TTCGAA 2 cut(s) 226, 1285
SinI GGWCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
Sse9I AATT 7 cut(s) 387, 705, 1038, 1105, 1162, 1194, 1281
SspI AATATT 1 cut(s) 1272
SspMI CTAG 5 cut(s) 263, 402, 617, 710, 978
StyI CCWWGG 1 cut(s) 977
TaaI ACNGT 7 cut(s) 313, 397, 471, 510, 526, 559, 991
TaiI ACGT 1 cut(s) 449
TaqI TCGA 3 cut(s) 226, 1265, 1285
TasI AATT 7 cut(s) 387, 705, 1038, 1105, 1162, 1194, 1281
TatI WGTACW 2 cut(s) 297, 1071
TfiI GAWTC 3 cut(s) 564, 1044, 1091
Tru1I TTAA 5 cut(s) 219, 828, 930, 1134, 1161
Tru9I TTAA 5 cut(s) 219, 828, 930, 1134, 1161
TscAI CASTG 3 cut(s) 400, 480, 961
TseFI GTSAC 3 cut(s) 471, 667, 1243
TseI GCWGC 7 cut(s) 405, 503, 548, 747, 761, 984, 1028
Tsp45I GTSAC 3 cut(s) 471, 667, 1243
TspDTI ATGAA 5 cut(s) 88, 143, 484, 616, 828
TspRI CASTG 3 cut(s) 400, 480, 961
VneI GTGCAC 2 cut(s) 640, 1216
VpaK11BI GGWCC 6 cut(s) 305, 555, 582, 656, 1062, 1232
XapI RAATTY 1 cut(s) 1105
XceI RCATGY 1 cut(s) 26
XmaJI CCTAGG 1 cut(s) 977
XmiI GTMKAC 1 cut(s) 141
XspI CTAG 5 cut(s) 263, 402, 617, 710, 978
Zsp2I ATGCAT 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.