Rroxscaffold_7G00180080

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002399
Physical Location & Seq
Forward (+)
19615060 .. 19616998
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00180080.1

Sequence Viewer

Length: 828 bp
ATGTATTACCGATGCATTACGGATGCATCCTTCAATCATGGGATGAATCCTCATGTCTTTGTAACCTATCGACATATAGTTGGTGGCTTAGCCATGTTTCCATATGCCTATTTTCTAGAAAGAAAAGCAAGGCCAAAGTTGACTCTAGCATTGTTTATGGAGATATTTGTGATTTCCCTTCTAGGGGTTAGCTTAACCCTTAATATGTATTTTGCAAGCCTGAAGTACACATCTTCAAGCTTCGTCACACCAATAGCCAATACCATACCATTCCTGACTTTCATGATTGCAGTCATACTCAGGATGGAAGCTCTAGATGTAAGGAATCCATGCGGAATAGCTAAACTGTTGGGAACATTAAGATCTCTGGCTGGGGTCATCACCATGACTTCGTACAAAGGACCTGCAATACAAAGCTTCTCAGGAGCATCACAACACATGAGAACGCAATCCTCTGTGTTCACAGTTATCATACAGCACAAACGGGCAGCATGGAGAATTAAATACAACAATGATTTCTGGTCCATAATATATGCTGGAGTTGTATGCTCAGCATTAGTGGCGATTTTAGCCTACTTCATTCTCAGCGAAAAGCTGCACATTGGCAGATTAGTGGGAGCGGTCATTATCATTATCGGTCTATACTTAGTCTTATGGGGCAAAGATAGAGATCAAAATCATTTCAAATCCCAAGAGCAACCTACTGTGATTAGTGATAACCAGAAGGAACCCAAGATGCAAGTGGGATCTTCAGCAGAAAGACAGGTCCTCCAAATGGACCTTGAGGAAAAAGATAAAACCCATGGTTCAAGATTATGCACCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

275

Amino Acids

30.99

Weight (kDa)

9.55

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 13 - 114 2.6e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 412
AccBSI CCGCTC 1 cut(s) 620
AciI CCGC 2 cut(s) 333, 620
AclWI GGATC 1 cut(s) 754
AcuI CTGAAG 2 cut(s) 242, 735
AfaI GTAC 2 cut(s) 227, 395
AfiI CCNNNNNNNGG 3 cut(s) 183, 184, 775
AgsI TTSAA 4 cut(s) 34, 237, 685, 810
AluBI AGCT 6 cut(s) 192, 240, 311, 341, 417, 595
AluI AGCT 6 cut(s) 192, 240, 311, 341, 417, 595
AlwI GGATC 1 cut(s) 754
AoxI GGCC 1 cut(s) 131
ApeKI GCWGC 2 cut(s) 488, 595
AspS9I GGNCC 4 cut(s) 401, 522, 766, 778
AsuHPI GGTGA 1 cut(s) 373
AvaII GGWCC 4 cut(s) 401, 522, 766, 778
BbvI GCAGC 2 cut(s) 500, 582
BccI CCATC 1 cut(s) 298
BfaI CTAG 4 cut(s) 116, 146, 182, 314
BfuAI ACCTGC 1 cut(s) 412
BglII AGATCT 1 cut(s) 362
BisI GCNGC 2 cut(s) 489, 596
BlpI GCTNAGC 2 cut(s) 88, 550
BlsI GCNGC 2 cut(s) 490, 597
Bme18I GGWCC 4 cut(s) 401, 522, 766, 778
BmgT120I GGNCC 4 cut(s) 401, 522, 766, 778
BmiI GGNNCC 1 cut(s) 729
BmsI GCATC 5 cut(s) 2, 13, 35, 437, 726
BpmI CTGGAG 1 cut(s) 558
Bpu1102I GCTNAGC 2 cut(s) 88, 550
BpuEI CTTGAG 1 cut(s) 803
BsaBI GATNNNNATC 2 cut(s) 669, 675
BsaJI CCNNGG 1 cut(s) 802
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 184, 775
Bse8I GATNNNNATC 2 cut(s) 669, 675
BseDI CCNNGG 1 cut(s) 802
BseGI GGATG 4 cut(s) 26, 28, 48, 309
BseJI GATNNNNATC 2 cut(s) 669, 675
BseLI CCNNNNNNNGG 3 cut(s) 183, 184, 775
BseMII CTCAG 4 cut(s) 313, 435, 564, 598
BseXI GCAGC 2 cut(s) 500, 582
BseYI CCCAGC 1 cut(s) 371
BsgI GTGCAG 1 cut(s) 581
BshFI GGCC 1 cut(s) 133
BslI CCNNNNNNNGG 3 cut(s) 183, 184, 775
BsnI GGCC 1 cut(s) 133
Bsp143I GATC 3 cut(s) 362, 670, 746
Bsp1720I GCTNAGC 2 cut(s) 88, 550
Bsp19I CCATGG 1 cut(s) 802
BspACI CCGC 2 cut(s) 333, 620
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 4 cut(s) 312, 434, 563, 597
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 1 cut(s) 729
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 1 cut(s) 754
BsrBI CCGCTC 1 cut(s) 620
BssECI CCNNGG 1 cut(s) 802
BssMI GATC 3 cut(s) 362, 670, 746
BssT1I CCWWGG 1 cut(s) 802
Bst4CI ACNGT 3 cut(s) 348, 466, 706
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 6 cut(s) 88, 299, 421, 550, 584, 646
BstDSI CCRYGG 1 cut(s) 802
BstF5I GGATG 4 cut(s) 26, 28, 48, 309
BstKTI GATC 3 cut(s) 365, 673, 749
BstMBI GATC 3 cut(s) 362, 670, 746
BstMWI GCNNNNNNNGC 2 cut(s) 560, 569
BstV1I GCAGC 2 cut(s) 500, 582
BstX2I RGATCY 2 cut(s) 362, 746
BstYI RGATCY 2 cut(s) 362, 746
BsuRI GGCC 1 cut(s) 133
BtgI CCRYGG 1 cut(s) 802
BtsCI GGATG 4 cut(s) 26, 28, 48, 309
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 1 cut(s) 217
CciI TCATGA 1 cut(s) 282
Cfr13I GGNCC 4 cut(s) 401, 522, 766, 778
Csp6I GTAC 2 cut(s) 226, 394
CviAII CATG 9 cut(s) 38, 53, 94, 283, 330, 385, 439, 492, 803
CviQI GTAC 2 cut(s) 226, 394
DdeI CTNAG 6 cut(s) 88, 299, 421, 550, 584, 646
DpnI GATC 3 cut(s) 364, 672, 748
DpnII GATC 3 cut(s) 362, 670, 746
Eco130I CCWWGG 1 cut(s) 802
Eco47I GGWCC 4 cut(s) 401, 522, 766, 778
Eco57I CTGAAG 2 cut(s) 242, 735
EcoO109I RGGNCCY 2 cut(s) 401, 766
EcoT14I CCWWGG 1 cut(s) 802
EcoT22I ATGCAT 2 cut(s) 17, 28
ErhI CCWWGG 1 cut(s) 802
FaeI CATG 9 cut(s) 41, 56, 97, 286, 333, 388, 442, 495, 806
FatI CATG 9 cut(s) 37, 52, 93, 282, 329, 384, 438, 491, 802
FauNDI CATATG 2 cut(s) 103, 824
Fnu4HI GCNGC 2 cut(s) 489, 596
FokI GGATG 4 cut(s) 13, 35, 55, 316
Fsp4HI GCNGC 2 cut(s) 489, 596
FspBI CTAG 4 cut(s) 116, 146, 182, 314
GluI GCNGC 2 cut(s) 489, 596
GsaI CCCAGC 1 cut(s) 375
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 1 cut(s) 133
Hin1II CATG 9 cut(s) 41, 56, 97, 286, 333, 388, 442, 495, 806
HincII GTYRAC 1 cut(s) 141
HindII GTYRAC 1 cut(s) 141
HindIII AAGCTT 2 cut(s) 238, 415
HinfI GANTC 3 cut(s) 46, 142, 325
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 3 cut(s) 141, 228, 462
Hpy188III TCNNGA 7 cut(s) 116, 274, 283, 301, 314, 423, 810
Hpy8I GTNNAC 3 cut(s) 141, 228, 462
HpyAV CCTTC 3 cut(s) 40, 188, 718
HpyCH4III ACNGT 3 cut(s) 348, 466, 706
HpyCH4V TGCA 8 cut(s) 15, 26, 215, 290, 407, 598, 739, 819
HpyF10VI GCNNNNNNNGC 2 cut(s) 560, 569
HpyF3I CTNAG 6 cut(s) 88, 299, 421, 550, 584, 646
Hsp92II CATG 9 cut(s) 41, 56, 97, 286, 333, 388, 442, 495, 806
Kzo9I GATC 3 cut(s) 362, 670, 746
LmnI GCTCC 2 cut(s) 425, 617
Lsp1109I GCAGC 2 cut(s) 500, 582
LweI GCATC 5 cut(s) 2, 13, 35, 437, 726
MaeI CTAG 4 cut(s) 116, 146, 182, 314
MaeIII GTNAC 2 cut(s) 61, 244
MalI GATC 3 cut(s) 364, 672, 748
MbiI CCGCTC 1 cut(s) 620
MboI GATC 3 cut(s) 362, 670, 746
MboII GAAGA 2 cut(s) 225, 741
MflI RGATCY 2 cut(s) 362, 746
MluCI AATT 1 cut(s) 498
MlyI GAGTC 1 cut(s) 136
MnlI CCTC 4 cut(s) 60, 463, 778, 779
Mph1103I ATGCAT 2 cut(s) 17, 28
MseI TTAA 4 cut(s) 194, 201, 359, 501
MslI CAYNNNNRTG 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 560, 569
NcoI CCATGG 1 cut(s) 802
NdeI CATATG 2 cut(s) 103, 824
NdeII GATC 3 cut(s) 362, 670, 746
NlaIII CATG 9 cut(s) 41, 56, 97, 286, 333, 388, 442, 495, 806
NlaIV GGNNCC 1 cut(s) 729
NmuCI GTSAC 1 cut(s) 244
NsiI ATGCAT 2 cut(s) 17, 28
PagI TCATGA 1 cut(s) 282
PfeI GAWTC 2 cut(s) 46, 325
PkrI GCNGC 2 cut(s) 490, 597
PleI GAGTC 1 cut(s) 136
PpsI GAGTC 1 cut(s) 136
PpuMI RGGWCCY 2 cut(s) 401, 766
Psp5II RGGWCCY 2 cut(s) 401, 766
PspFI CCCAGC 1 cut(s) 371
PspN4I GGNNCC 1 cut(s) 729
PspPI GGNCC 4 cut(s) 401, 522, 766, 778
PspPPI RGGWCCY 2 cut(s) 401, 766
PsuI RGATCY 2 cut(s) 362, 746
RsaI GTAC 2 cut(s) 227, 395
RsaNI GTAC 2 cut(s) 226, 394
RseI CAYNNNNRTG 1 cut(s) 383
SaqAI TTAA 4 cut(s) 194, 201, 359, 501
SatI GCNGC 2 cut(s) 489, 596
Sau3AI GATC 3 cut(s) 362, 670, 746
Sau96I GGNCC 4 cut(s) 401, 522, 766, 778
SchI GAGTC 1 cut(s) 136
SfaNI GCATC 5 cut(s) 2, 13, 35, 437, 726
SinI GGWCC 4 cut(s) 401, 522, 766, 778
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 782
SmoI CTYRAG 1 cut(s) 782
Sse9I AATT 1 cut(s) 498
SsiI CCGC 2 cut(s) 333, 620
SspMI CTAG 4 cut(s) 116, 146, 182, 314
StyI CCWWGG 1 cut(s) 802
TaaI ACNGT 3 cut(s) 348, 466, 706
TaqI TCGA 1 cut(s) 70
TaqII GACCGA 1 cut(s) 626
TasI AATT 1 cut(s) 498
TatI WGTACW 1 cut(s) 225
TfiI GAWTC 2 cut(s) 46, 325
Tru1I TTAA 4 cut(s) 194, 201, 359, 501
Tru9I TTAA 4 cut(s) 194, 201, 359, 501
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 2 cut(s) 488, 595
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 3 cut(s) 59, 271, 568
TspGWI ACGGA 1 cut(s) 35
VpaK11BI GGWCC 4 cut(s) 401, 522, 766, 778
XbaI TCTAGA 2 cut(s) 115, 313
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XspI CTAG 4 cut(s) 116, 146, 182, 314
Zsp2I ATGCAT 2 cut(s) 17, 28
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.