Rh6BG313300

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0002399
Physical Location & Seq
Reverse (-)
54326370 .. 54330099
3730 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG313300.1

Sequence Viewer

Length: 930 bp
ATGAATTTGAGCTTCAAGTTTAATTTCCCTGCTGGAAAATCTCACTTTTTTTTTCTCTTGATGCAAAGCAGGGTTAGCTTAACCCTTAATATGTATTTTGCAAGCCTGAAGTACACATCTTCAAGCTTCGTCACATCAATAGCCAATACCATACCATCCCTGACTTTTTTGATTGCAGTCATACTCAGGATGGAGGCTCTAGATGTAAGGAATCTGCGCGGAATAGCTAAACTCTTGGGAACCTTAATATCTCTGGCTGGGGTCATCACCATGACTTCGTACAAAGGACCTGCAATACAAAGCTTCTCAGGAGCATCACAACACATAAGAAGTAATTTTGTTCACAAAAGCTGGACAAAGGGTTCAATCCTTGTGATTGCAAGTTGTATAACATGGTCCATATGGTACATAATGCAGGGGATTACATTGAAGAAATATCCTGCACAACTGTCACTAACCACATGGATCAATTGTATCGCTGCAGCACAATCCGCTGTGTTCACAGTTATAATACAGCACAAACGGGAAGCATGGAGAATTACATACAACAATGATTTCTGGTCCATCATATATGCTGGAGTTGTATGCTCAGGTATAACAATCTTCATTCAACTATGGTGTACAAAACAAAAAGGACCTGTCTTCGTGACCATGTTCAGTCCTCTTGCAACAGCATTAGTGGCTATTCTAGCTTACTTCATTCTCGGCGAAAAGCTGCACATTGGCAGAATAGTGGGAGCGGTCATTATCATTATCGGTCTATACTTAGTCTTATGGGGCAAAGATAGAGATCAAAGTCATTTCAAATCCCAAGAGCAACCTACCGTGATTAGTGATAACCAGAAGGAACCCAAGATGCAAGTGGGATCTTCAGCAGAAAGACAGGTCCTCCAAATGGACCTTGAGGAAAAAGATAAAACCGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

309

Amino Acids

34.58

Weight (kDa)

9.65

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 120 - 258 2e-17 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 509
Acc36I ACCTGC 1 cut(s) 298
AccBSI CCGCTC 1 cut(s) 740
AccII CGCG 1 cut(s) 219
AciI CCGC 3 cut(s) 219, 492, 740
AclWI GGATC 2 cut(s) 473, 874
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 2 cut(s) 128, 855
AfaI GTAC 4 cut(s) 113, 281, 407, 622
AfiI CCNNNNNNNGG 1 cut(s) 895
AgsI TTSAA 6 cut(s) 16, 123, 366, 430, 611, 805
AloI GAACNNNNNNTCC 2 cut(s) 346, 378
AluBI AGCT 8 cut(s) 12, 78, 126, 227, 303, 351, 692, 715
AluI AGCT 8 cut(s) 12, 78, 126, 227, 303, 351, 692, 715
AlwI GGATC 2 cut(s) 473, 874
ApeKI GCWGC 3 cut(s) 479, 482, 715
ApoI RAATTY 1 cut(s) 4
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 6 cut(s) 287, 396, 561, 635, 886, 898
AsuHPI GGTGA 1 cut(s) 259
AvaII GGWCC 6 cut(s) 287, 396, 561, 635, 886, 898
BbsI GAAGAC 1 cut(s) 634
BbvI GCAGC 3 cut(s) 466, 494, 702
BccI CCATC 4 cut(s) 163, 184, 572, 917
BfaI CTAG 2 cut(s) 200, 689
BfmI CTRYAG 1 cut(s) 480
BfuAI ACCTGC 1 cut(s) 298
BisI GCNGC 3 cut(s) 480, 483, 716
BlsI GCNGC 3 cut(s) 481, 484, 717
Bme18I GGWCC 6 cut(s) 287, 396, 561, 635, 886, 898
BmgT120I GGNCC 6 cut(s) 287, 396, 561, 635, 886, 898
BmiI GGNNCC 2 cut(s) 241, 849
BmsI GCATC 3 cut(s) 51, 323, 846
BpiI GAAGAC 1 cut(s) 634
BpmI CTGGAG 1 cut(s) 597
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 1 cut(s) 923
BsaBI GATNNNNATC 1 cut(s) 789
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 1 cut(s) 895
Bse8I GATNNNNATC 1 cut(s) 789
BseGI GGATG 2 cut(s) 155, 195
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 1 cut(s) 895
BseMII CTCAG 3 cut(s) 199, 321, 603
BseXI GCAGC 3 cut(s) 466, 494, 702
BseYI CCCAGC 1 cut(s) 257
BsgI GTGCAG 2 cut(s) 426, 701
Bsh1236I CGCG 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 895
Bsp1407I TGTACA 1 cut(s) 620
Bsp143I GATC 3 cut(s) 465, 790, 866
BspACI CCGC 3 cut(s) 219, 492, 740
BspCNI CTCAG 3 cut(s) 198, 320, 602
BspFNI CGCG 1 cut(s) 219
BspLI GGNNCC 2 cut(s) 241, 849
BspMAI CTGCAG 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 298
BspPI GGATC 2 cut(s) 473, 874
BsrBI CCGCTC 1 cut(s) 740
BsrGI TGTACA 1 cut(s) 620
BssMI GATC 3 cut(s) 465, 790, 866
Bst4CI ACNGT 3 cut(s) 450, 505, 826
BstAUI TGTACA 1 cut(s) 620
BstC8I GCNNGC 1 cut(s) 103
BstDEI CTNAG 4 cut(s) 185, 307, 589, 766
BstF5I GGATG 2 cut(s) 155, 195
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 3 cut(s) 468, 793, 869
BstMBI GATC 3 cut(s) 465, 790, 866
BstMWI GCNNNNNNNGC 4 cut(s) 75, 491, 680, 689
BstSFI CTRYAG 1 cut(s) 480
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 3 cut(s) 466, 494, 702
BstV2I GAAGAC 1 cut(s) 634
BstX2I RGATCY 1 cut(s) 866
BstYI RGATCY 1 cut(s) 866
BtsCI GGATG 2 cut(s) 155, 195
BveI ACCTGC 1 cut(s) 298
Cac8I GCNNGC 1 cut(s) 103
CfoI GCGC 1 cut(s) 219
Cfr13I GGNCC 6 cut(s) 287, 396, 561, 635, 886, 898
Csp6I GTAC 4 cut(s) 112, 280, 406, 621
CviAII CATG 5 cut(s) 271, 393, 462, 531, 652
CviQI GTAC 4 cut(s) 112, 280, 406, 621
DdeI CTNAG 4 cut(s) 185, 307, 589, 766
DpnI GATC 3 cut(s) 467, 792, 868
DpnII GATC 3 cut(s) 465, 790, 866
Eco47I GGWCC 6 cut(s) 287, 396, 561, 635, 886, 898
Eco57I CTGAAG 2 cut(s) 128, 855
EcoO109I RGGNCCY 3 cut(s) 287, 635, 886
FaeI CATG 5 cut(s) 274, 396, 465, 534, 655
FatI CATG 5 cut(s) 270, 392, 461, 530, 651
FauNDI CATATG 1 cut(s) 401
Fnu4HI GCNGC 3 cut(s) 480, 483, 716
FokI GGATG 2 cut(s) 142, 202
Fsp4HI GCNGC 3 cut(s) 480, 483, 716
FspBI CTAG 2 cut(s) 200, 689
GlaI GCGC 1 cut(s) 218
GluI GCNGC 3 cut(s) 480, 483, 716
GsaI CCCAGC 1 cut(s) 261
GsuI CTGGAG 1 cut(s) 597
HhaI GCGC 1 cut(s) 219
Hin1II CATG 5 cut(s) 274, 396, 465, 534, 655
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 2 cut(s) 124, 301
HinfI GANTC 1 cut(s) 211
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 4 cut(s) 114, 343, 501, 621
Hpy188III TCNNGA 5 cut(s) 58, 187, 200, 309, 646
Hpy8I GTNNAC 4 cut(s) 114, 343, 501, 621
HpyAV CCTTC 1 cut(s) 838
HpyCH4III ACNGT 3 cut(s) 450, 505, 826
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 491, 680, 689
HpyF3I CTNAG 4 cut(s) 185, 307, 589, 766
Hsp92II CATG 5 cut(s) 274, 396, 465, 534, 655
HspAI GCGC 1 cut(s) 217
Kzo9I GATC 3 cut(s) 465, 790, 866
LmnI GCTCC 2 cut(s) 311, 737
Lsp1109I GCAGC 3 cut(s) 466, 494, 702
LweI GCATC 3 cut(s) 51, 323, 846
MaeI CTAG 2 cut(s) 200, 689
MaeIII GTNAC 3 cut(s) 130, 450, 646
MalI GATC 3 cut(s) 467, 792, 868
MbiI CCGCTC 1 cut(s) 740
MboI GATC 3 cut(s) 465, 790, 866
MboII GAAGA 5 cut(s) 111, 442, 595, 634, 861
MfeI CAATTG 1 cut(s) 469
MflI RGATCY 1 cut(s) 866
MluCI AATT 5 cut(s) 4, 22, 334, 469, 537
MnlI CCTC 4 cut(s) 187, 672, 898, 899
MseI TTAA 4 cut(s) 21, 80, 87, 245
MslI CAYNNNNRTG 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 494
MunI CAATTG 1 cut(s) 469
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 4 cut(s) 75, 491, 680, 689
NdeI CATATG 1 cut(s) 401
NdeII GATC 3 cut(s) 465, 790, 866
NlaIII CATG 5 cut(s) 274, 396, 465, 534, 655
NlaIV GGNNCC 2 cut(s) 241, 849
NmeAIII GCCGAG 1 cut(s) 684
NmuCI GTSAC 3 cut(s) 130, 450, 646
PfeI GAWTC 1 cut(s) 211
PkrI GCNGC 3 cut(s) 481, 484, 717
PpuMI RGGWCCY 3 cut(s) 287, 635, 886
PsiI TTATAA 1 cut(s) 509
Psp5II RGGWCCY 3 cut(s) 287, 635, 886
PspFI CCCAGC 1 cut(s) 257
PspN4I GGNNCC 2 cut(s) 241, 849
PspPI GGNCC 6 cut(s) 287, 396, 561, 635, 886, 898
PspPPI RGGWCCY 3 cut(s) 287, 635, 886
PstI CTGCAG 1 cut(s) 484
PsuI RGATCY 1 cut(s) 866
RsaI GTAC 4 cut(s) 113, 281, 407, 622
RsaNI GTAC 4 cut(s) 112, 280, 406, 621
RseI CAYNNNNRTG 1 cut(s) 269
SaqAI TTAA 4 cut(s) 21, 80, 87, 245
SatI GCNGC 3 cut(s) 480, 483, 716
Sau3AI GATC 3 cut(s) 465, 790, 866
Sau96I GGNCC 6 cut(s) 287, 396, 561, 635, 886, 898
SfaNI GCATC 3 cut(s) 51, 323, 846
SfcI CTRYAG 1 cut(s) 480
SinI GGWCC 6 cut(s) 287, 396, 561, 635, 886, 898
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
Sse9I AATT 5 cut(s) 4, 22, 334, 469, 537
SsiI CCGC 3 cut(s) 219, 492, 740
SspMI CTAG 2 cut(s) 200, 689
TaaI ACNGT 3 cut(s) 450, 505, 826
TaqII GACCGA 1 cut(s) 746
TasI AATT 5 cut(s) 4, 22, 334, 469, 537
TatI WGTACW 2 cut(s) 111, 620
TfiI GAWTC 1 cut(s) 211
Tru1I TTAA 4 cut(s) 21, 80, 87, 245
Tru9I TTAA 4 cut(s) 21, 80, 87, 245
TseFI GTSAC 3 cut(s) 130, 450, 646
TseI GCWGC 3 cut(s) 479, 482, 715
Tsp45I GTSAC 3 cut(s) 130, 450, 646
TspDTI ATGAA 3 cut(s) 17, 595, 688
VpaK11BI GGWCC 6 cut(s) 287, 396, 561, 635, 886, 898
XapI RAATTY 1 cut(s) 4
XbaI TCTAGA 1 cut(s) 199
XcmI CCANNNNNNNNNTGG 1 cut(s) 859
XspI CTAG 2 cut(s) 200, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.