RchiOBHm_Chr4g0392391

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002399
Physical Location & Seq
Forward (+)
7805404 .. 7807756
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36515

Sequence Viewer

Length: 1044 bp
ATGGCACAAGAGCCAAGTTCCCATGTGGGAACTTACAAGAGGTTCAAGCCACACCTCCTCATGGTTTTATCTCAAGTGGCCTACGCATTTCTCTATTTCATTACAGAAGCTTCCTTCAATCATGGGATGAACCCTCATGTCTACATAACTTATCGACATATCGTGTCTGGCATAATCATGCTCCCTTTTGCCTATTTTCTTGAAAGGATAGGTTTGACTTTAAACATGTACTTTGCAAGCTTAAGATACACCTCTCCAACATTCCTTGCATCAATGATCAACACCATAGCTTCCCTCACCTTTGTGATTGCAGTTTCACTCAAGTTGGAGGTTCTTGATCTTCTAAATCCTCGTGGATTAGCAAAAGTTTTGGGCACCATAGTCTCCTTAGCCGGTGTAATGACCATGACTTTGTACAAAGGACCTATTATGAGAAACTTGTGGAGTCCACTAATCCATATTCAAGGAAAAACTTCCATCCATGAGAACTGGTTGAAGGGTTCAATTCTAACTGTTGCTAGCTGCATAACATGGTCTTTATGGTACATTATGCAGGCAGTCACATTGAAAAGATATCCTGCACAACTATCAATGATAACATGGATGAGCTTCCTAGGGGCAGCACAGTCAGCTGTCTTTGCAGTTTGTATAGAACATAAACGAGCTGCTTGGACAGTTGGATTCAACATTGACCTCTGGTGCATTTTGTATTCTGGAGGGGTGTGCTCTGGCCTAGTACTCTTTATTCAACTATGGTGCACAGTAGAGAAAGGACCTGTCTTTGTGACCATGTTTGATCCCGTCTCAACAATATTGGTGGCAATTCTAGCATACTTTGTGCTTGGTGAAAGACTCTATATTGGCAGCATAGTAGGCGCAGCTATCGTCATTGTTGGCCTATACTTGCTGTTGTGGGGAAAAGAAGGTGATGAAGTTTACACCGCTAAGTCTGAAGAGTCTGATCAGACAAATGAAGAGGAGAAAGATAACAATATGCCGACGATTTCTTCAGTCAAGAAGGATGCATTACATGATCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

347

Amino Acids

38.79

Weight (kDa)

7.67

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 166 - 304 1.5e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 374
AccI GTMKAC 1 cut(s) 141
AciI CCGC 1 cut(s) 942
AclWI GGATC 1 cut(s) 791
AcuI CTGAAG 2 cut(s) 972, 993
AfaI GTAC 4 cut(s) 230, 416, 545, 738
AfiI CCNNNNNNNGG 1 cut(s) 61
AflII CTTAAG 1 cut(s) 241
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 9 cut(s) 46, 118, 203, 464, 496, 504, 568, 685, 749
AleI CACNNNNGTG 1 cut(s) 302
AluBI AGCT 8 cut(s) 110, 240, 290, 522, 609, 632, 665, 881
AluI AGCT 8 cut(s) 110, 240, 290, 522, 609, 632, 665, 881
Alw21I GWGCWC 2 cut(s) 728, 761
Alw26I GTCTC 2 cut(s) 388, 808
Alw44I GTGCAC 1 cut(s) 757
AlwI GGATC 1 cut(s) 791
AoxI GGCC 3 cut(s) 78, 730, 895
ApaLI GTGCAC 1 cut(s) 757
ApeKI GCWGC 5 cut(s) 522, 620, 665, 864, 878
ArsI GACNNNNNNTTYG 2 cut(s) 394, 426
Asp700I GAANNNNTTC 1 cut(s) 472
AspA2I CCTAGG 1 cut(s) 613
AspLEI GCGC 1 cut(s) 878
AspS9I GGNCC 2 cut(s) 422, 773
AsuHPI GGTGA 3 cut(s) 289, 857, 938
AsuNHI GCTAGC 1 cut(s) 518
AvaII GGWCC 2 cut(s) 422, 773
AvrII CCTAGG 1 cut(s) 613
BaeGI GKGCMC 2 cut(s) 377, 761
BanI GGYRCC 1 cut(s) 374
BauI CACGAG 1 cut(s) 351
Bbv12I GWGCWC 2 cut(s) 728, 761
BbvI GCAGC 5 cut(s) 509, 632, 652, 876, 890
BccI CCATC 1 cut(s) 485
BclI TGATCA 3 cut(s) 276, 961, 1033
BcoDI GTCTC 2 cut(s) 388, 808
BfaI CTAG 4 cut(s) 519, 614, 734, 827
BfrI CTTAAG 1 cut(s) 241
BisI GCNGC 5 cut(s) 523, 621, 666, 865, 879
BlnI CCTAGG 1 cut(s) 613
BlsI GCNGC 5 cut(s) 524, 622, 667, 866, 880
BmcAI AGTACT 1 cut(s) 738
Bme18I GGWCC 2 cut(s) 422, 773
BmgT120I GGNCC 2 cut(s) 422, 773
BmiI GGNNCC 1 cut(s) 376
BmsI GCATC 2 cut(s) 278, 1012
BmtI GCTAGC 1 cut(s) 522
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 1 cut(s) 388
BpuEI CTTGAG 2 cut(s) 57, 305
BsaJI CCNNGG 1 cut(s) 613
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 392
Bse1I ACTGG 1 cut(s) 494
BseDI CCNNGG 1 cut(s) 613
BseGI GGATG 4 cut(s) 132, 477, 609, 1027
BseLI CCNNNNNNNGG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 2 cut(s) 47, 992
BseSI GKGCMC 2 cut(s) 377, 761
BseXI GCAGC 5 cut(s) 509, 632, 652, 876, 890
BsgI GTGCAG 1 cut(s) 564
BshFI GGCC 3 cut(s) 80, 732, 897
BshNI GGYRCC 1 cut(s) 374
BsiHKAI GWGCWC 2 cut(s) 728, 761
BsiSI CCGG 1 cut(s) 393
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 2 cut(s) 388, 808
BsmBI CGTCTC 1 cut(s) 808
BsnI GGCC 3 cut(s) 80, 732, 897
Bsp1286I GDGCHC 3 cut(s) 377, 728, 761
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 5 cut(s) 276, 337, 796, 961, 1033
BspACI CCGC 1 cut(s) 942
BspANI GGCC 3 cut(s) 80, 732, 897
BspLI GGNNCC 1 cut(s) 376
BspOI GCTAGC 1 cut(s) 522
BspPI GGATC 1 cut(s) 791
BspT107I GGYRCC 1 cut(s) 374
BspTI CTTAAG 1 cut(s) 241
BsrFI RCCGGY 1 cut(s) 392
BsrGI TGTACA 1 cut(s) 414
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 1 cut(s) 613
BssMI GATC 5 cut(s) 276, 337, 796, 961, 1033
BssSI CACGAG 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 613
Bst2BI CACGAG 1 cut(s) 351
Bst4CI ACNGT 4 cut(s) 514, 627, 676, 763
Bst6I CTCTTC 2 cut(s) 948, 969
BstAFI CTTAAG 1 cut(s) 241
BstAUI TGTACA 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 238, 520, 555
BstDEI CTNAG 2 cut(s) 388, 945
BstF5I GGATG 4 cut(s) 132, 477, 609, 1027
BstHHI GCGC 1 cut(s) 878
BstKTI GATC 5 cut(s) 279, 340, 799, 964, 1036
BstMAI GTCTC 2 cut(s) 388, 808
BstMBI GATC 5 cut(s) 276, 337, 796, 961, 1033
BstMWI GCNNNNNNNGC 4 cut(s) 629, 638, 827, 873
BstNSI RCATGY 1 cut(s) 229
BstSLI GKGCMC 2 cut(s) 377, 761
BstV1I GCAGC 5 cut(s) 509, 632, 652, 876, 890
BsuRI GGCC 3 cut(s) 80, 732, 897
BtsCI GGATG 4 cut(s) 132, 477, 609, 1027
Cac8I GCNNGC 3 cut(s) 238, 520, 555
CfoI GCGC 1 cut(s) 878
Cfr10I RCCGGY 1 cut(s) 392
Cfr13I GGNCC 2 cut(s) 422, 773
Csp6I GTAC 4 cut(s) 229, 415, 544, 737
CspCI CAANNNNNGTGG 2 cut(s) 798, 833
CviQI GTAC 4 cut(s) 229, 415, 544, 737
DdeI CTNAG 2 cut(s) 388, 945
DpnI GATC 5 cut(s) 278, 339, 798, 963, 1035
DpnII GATC 5 cut(s) 276, 337, 796, 961, 1033
DraI TTTAAA 1 cut(s) 222
Eam1104I CTCTTC 2 cut(s) 948, 969
EarI CTCTTC 2 cut(s) 948, 969
Eco130I CCWWGG 1 cut(s) 613
Eco32I GATATC 1 cut(s) 575
Eco47I GGWCC 2 cut(s) 422, 773
Eco57I CTGAAG 2 cut(s) 972, 993
EcoO109I RGGNCCY 2 cut(s) 422, 773
EcoRV GATATC 1 cut(s) 575
EcoT14I CCWWGG 1 cut(s) 613
EcoT22I ATGCAT 1 cut(s) 1027
ErhI CCWWGG 1 cut(s) 613
Esp3I CGTCTC 1 cut(s) 808
FbaI TGATCA 3 cut(s) 276, 961, 1033
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 5 cut(s) 523, 621, 666, 865, 879
FokI GGATG 4 cut(s) 139, 464, 616, 1034
Fsp4HI GCNGC 5 cut(s) 523, 621, 666, 865, 879
FspBI CTAG 4 cut(s) 519, 614, 734, 827
GlaI GCGC 1 cut(s) 877
GluI GCNGC 5 cut(s) 523, 621, 666, 865, 879
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 3 cut(s) 80, 732, 897
HapII CCGG 1 cut(s) 393
HhaI GCGC 1 cut(s) 878
Hin6I GCGC 1 cut(s) 876
HinP1I GCGC 1 cut(s) 876
HindIII AAGCTT 2 cut(s) 108, 238
HinfI GANTC 4 cut(s) 445, 681, 852, 956
HpaII CCGG 1 cut(s) 393
HphI GGTGA 3 cut(s) 289, 857, 938
Hpy166II GTNNAC 4 cut(s) 142, 449, 759, 937
Hpy188I TCNGA 3 cut(s) 952, 961, 966
Hpy188III TCNNGA 4 cut(s) 200, 335, 714, 1015
Hpy8I GTNNAC 4 cut(s) 142, 449, 759, 937
Hpy99I CGWCG 1 cut(s) 1003
HpyAV CCTTC 4 cut(s) 124, 490, 917, 1012
HpyCH4III ACNGT 4 cut(s) 514, 627, 676, 763
HpyF10VI GCNNNNNNNGC 4 cut(s) 629, 638, 827, 873
HpyF3I CTNAG 2 cut(s) 388, 945
HspAI GCGC 1 cut(s) 876
Ksp22I TGATCA 3 cut(s) 276, 961, 1033
Kzo9I GATC 5 cut(s) 276, 337, 796, 961, 1033
LmnI GCTCC 1 cut(s) 186
LpnPI CCDG 9 cut(s) 153, 406, 475, 539, 591, 682, 699, 714, 789
Lsp1109I GCAGC 5 cut(s) 509, 632, 652, 876, 890
LweI GCATC 2 cut(s) 278, 1012
MaeI CTAG 4 cut(s) 519, 614, 734, 827
MaeIII GTNAC 2 cut(s) 559, 784
MalI GATC 5 cut(s) 278, 339, 798, 963, 1035
MboI GATC 5 cut(s) 276, 337, 796, 961, 1033
MboII GAAGA 4 cut(s) 332, 965, 986, 999
MhlI GDGCHC 3 cut(s) 377, 728, 761
MluCI AATT 2 cut(s) 504, 822
MlyI GAGTC 3 cut(s) 454, 846, 965
MmeI TCCRAC 3 cut(s) 281, 306, 658
Mph1103I ATGCAT 1 cut(s) 1027
MroXI GAANNNNTTC 1 cut(s) 472
MseI TTAA 2 cut(s) 221, 242
MslI CAYNNNNRTG 2 cut(s) 176, 302
MspA1I CMGCKG 1 cut(s) 632
MspCI CTTAAG 1 cut(s) 241
MspI CCGG 1 cut(s) 393
MwoI GCNNNNNNNGC 4 cut(s) 629, 638, 827, 873
NdeII GATC 5 cut(s) 276, 337, 796, 961, 1033
NheI GCTAGC 1 cut(s) 518
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 2 cut(s) 559, 784
NsiI ATGCAT 1 cut(s) 1027
NspI RCATGY 1 cut(s) 229
OliI CACNNNNGTG 1 cut(s) 302
PciI ACATGT 1 cut(s) 225
PdmI GAANNNNTTC 1 cut(s) 472
PfeI GAWTC 1 cut(s) 681
PkrI GCNGC 5 cut(s) 524, 622, 667, 866, 880
PleI GAGTC 3 cut(s) 453, 846, 964
PpsI GAGTC 3 cut(s) 453, 846, 964
PpuMI RGGWCCY 2 cut(s) 422, 773
PscI ACATGT 1 cut(s) 225
Psp5II RGGWCCY 2 cut(s) 422, 773
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 2 cut(s) 422, 773
PspPPI RGGWCCY 2 cut(s) 422, 773
PvuII CAGCTG 1 cut(s) 632
RsaI GTAC 4 cut(s) 230, 416, 545, 738
RsaNI GTAC 4 cut(s) 229, 415, 544, 737
RseI CAYNNNNRTG 2 cut(s) 176, 302
SaqAI TTAA 2 cut(s) 221, 242
SatI GCNGC 5 cut(s) 523, 621, 666, 865, 879
Sau3AI GATC 5 cut(s) 276, 337, 796, 961, 1033
Sau96I GGNCC 2 cut(s) 422, 773
ScaI AGTACT 1 cut(s) 738
SchI GAGTC 3 cut(s) 454, 846, 965
SduI GDGCHC 3 cut(s) 377, 728, 761
SfaNI GCATC 2 cut(s) 278, 1012
SinI GGWCC 2 cut(s) 422, 773
SmiMI CAYNNNNRTG 2 cut(s) 176, 302
SmlI CTYRAG 3 cut(s) 72, 241, 320
SmoI CTYRAG 3 cut(s) 72, 241, 320
Sse9I AATT 2 cut(s) 504, 822
SsiI CCGC 1 cut(s) 942
SspI AATATT 1 cut(s) 813
SspMI CTAG 4 cut(s) 519, 614, 734, 827
StyI CCWWGG 1 cut(s) 613
TaaI ACNGT 4 cut(s) 514, 627, 676, 763
TaqI TCGA 1 cut(s) 154
TasI AATT 2 cut(s) 504, 822
TatI WGTACW 3 cut(s) 228, 414, 736
TfiI GAWTC 1 cut(s) 681
Tru1I TTAA 2 cut(s) 221, 242
Tru9I TTAA 2 cut(s) 221, 242
TseFI GTSAC 2 cut(s) 559, 784
TseI GCWGC 5 cut(s) 522, 620, 665, 864, 878
Tsp45I GTSAC 2 cut(s) 559, 784
TspDTI ATGAA 4 cut(s) 88, 143, 945, 987
Vha464I CTTAAG 1 cut(s) 241
VneI GTGCAC 1 cut(s) 757
VpaK11BI GGWCC 2 cut(s) 422, 773
XceI RCATGY 1 cut(s) 229
XmaJI CCTAGG 1 cut(s) 613
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 4 cut(s) 519, 614, 734, 827
ZrmI AGTACT 1 cut(s) 738
Zsp2I ATGCAT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.