Rh4DG039600

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002399
Physical Location & Seq
Forward (+)
6425101 .. 6427427
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG039600.1

Sequence Viewer

Length: 939 bp
ATGGCACAAGAGCCAAGTTCCCATGTGGGAACTTACAAGAGGTTCAAGCCACACCTCCTCATGGTTTTATCTCAAGTGGCCTACGCATTTCTCTATTTCATTACAGAAGCTTCCTTCAATCATGGGATGAACCCTCATGTCTACATAACTTATCGACATATCGTGTCTGGCATAATCATGCTCCCTTTTGCCTATTTTCTTGAAAGAAAAGACAGACCAAAGCTCACATTTGCTCTTTTCTTGGAACTTTTTGCTATTTCTTTTCTGGGGATAGGTTTGACTTTAAACATGTACTTTGCAAGCTTAAGATACACCTCTCCAACCTTCCTTGCATCAATGATCAACACCATAGCTTCCCTCACTTTTGTGATTGCAGTTTCACTCAAGTTGGAGGTTCTTGATCTTCGAAATCCTCGTGGATTAGCAAAAGTTTTGGGCACCGTAATCTCCTTAGCCGGTGTAATGACCATGACTTTGTACAAAGGACCTATTATGAGAAACTTGTGGAGTCCACTAATCCATATTCAAGGAAAAACTTCCATCCATGAGAACTGGTTGAAGGGTTCAATTCTAACTGTTGCTAGCTGCATAACATGGTCTTTATGGTACATTATGCAGGCAGTCACATTGAAAAGATATCCTGCACAACTATCAATGATAACATGGATGAGCTTCCTAGGGGCAGCACAGTCAGCTGTCTTTGCAGTTTGTATAGAACATAAACGAGCTGCTTGGACAGTTGGATTCAACATCGACCTCTGCATAGTAGGCGCAGCTATTGTCATTGTTGGCCTATACTTGCTGTTGTGGGGAAAAGAAGGTGATGAAGTTTACACCACTAAGTCTGAAGAGTCTGATCAGACAAATGAAGAGGAGAAAGATAACAATATGCCGACGATTTCTTCAGTCAAGAAGGATGCATTACATGATCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

312

Amino Acids

35.1

Weight (kDa)

8.83

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 18 - 158 1e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 141
AcuI CTGAAG 2 cut(s) 867, 888
AfaI GTAC 3 cut(s) 293, 479, 608
AfiI CCNNNNNNNGG 1 cut(s) 61
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 8 cut(s) 46, 118, 203, 527, 559, 567, 631, 748
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 9 cut(s) 110, 223, 303, 353, 585, 672, 695, 728, 776
AluI AGCT 9 cut(s) 110, 223, 303, 353, 585, 672, 695, 728, 776
AoxI GGCC 2 cut(s) 78, 790
ApeKI GCWGC 4 cut(s) 585, 683, 728, 773
ArsI GACNNNNNNTTYG 2 cut(s) 457, 489
Asp700I GAANNNNTTC 1 cut(s) 535
AspA2I CCTAGG 1 cut(s) 676
AspLEI GCGC 1 cut(s) 773
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 833
AsuII TTCGAA 1 cut(s) 406
AsuNHI GCTAGC 1 cut(s) 581
AvaII GGWCC 1 cut(s) 485
AvrII CCTAGG 1 cut(s) 676
BaeGI GKGCMC 1 cut(s) 440
BanI GGYRCC 1 cut(s) 437
BauI CACGAG 1 cut(s) 414
BbvI GCAGC 4 cut(s) 572, 695, 715, 785
BccI CCATC 1 cut(s) 548
BclI TGATCA 3 cut(s) 339, 856, 928
BfaI CTAG 2 cut(s) 582, 677
BfrI CTTAAG 1 cut(s) 304
BisI GCNGC 4 cut(s) 586, 684, 729, 774
BlnI CCTAGG 1 cut(s) 676
BlsI GCNGC 4 cut(s) 587, 685, 730, 775
Bme18I GGWCC 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 439
BmsI GCATC 2 cut(s) 341, 907
BmtI GCTAGC 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 451
Bpu14I TTCGAA 1 cut(s) 406
BpuEI CTTGAG 2 cut(s) 57, 368
BsaJI CCNNGG 1 cut(s) 676
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 1 cut(s) 557
BseDI CCNNGG 1 cut(s) 676
BseGI GGATG 4 cut(s) 132, 540, 672, 922
BseLI CCNNNNNNNGG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 2 cut(s) 47, 887
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 4 cut(s) 572, 695, 715, 785
BsgI GTGCAG 1 cut(s) 627
BshFI GGCC 2 cut(s) 80, 792
BshNI GGYRCC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 1 cut(s) 61
BsnI GGCC 2 cut(s) 80, 792
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 440
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 4 cut(s) 339, 400, 856, 928
BspANI GGCC 2 cut(s) 80, 792
BspLI GGNNCC 1 cut(s) 439
BspOI GCTAGC 1 cut(s) 585
BspT104I TTCGAA 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 437
BspTI CTTAAG 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 455
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 1 cut(s) 676
BssMI GATC 4 cut(s) 339, 400, 856, 928
BssSI CACGAG 1 cut(s) 414
BssT1I CCWWGG 1 cut(s) 676
Bst2BI CACGAG 1 cut(s) 414
Bst4CI ACNGT 4 cut(s) 442, 577, 690, 739
Bst6I CTCTTC 2 cut(s) 843, 864
BstAFI CTTAAG 1 cut(s) 304
BstAUI TGTACA 1 cut(s) 477
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 3 cut(s) 301, 583, 618
BstDEI CTNAG 2 cut(s) 451, 840
BstF5I GGATG 4 cut(s) 132, 540, 672, 922
BstHHI GCGC 1 cut(s) 773
BstKTI GATC 4 cut(s) 342, 403, 859, 931
BstMBI GATC 4 cut(s) 339, 400, 856, 928
BstMWI GCNNNNNNNGC 3 cut(s) 692, 701, 768
BstNSI RCATGY 1 cut(s) 292
BstSLI GKGCMC 1 cut(s) 440
BstV1I GCAGC 4 cut(s) 572, 695, 715, 785
BsuRI GGCC 2 cut(s) 80, 792
BtsCI GGATG 4 cut(s) 132, 540, 672, 922
Cac8I GCNNGC 3 cut(s) 301, 583, 618
CfoI GCGC 1 cut(s) 773
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 485
Csp6I GTAC 3 cut(s) 292, 478, 607
CviQI GTAC 3 cut(s) 292, 478, 607
DdeI CTNAG 2 cut(s) 451, 840
DpnI GATC 4 cut(s) 341, 402, 858, 930
DpnII GATC 4 cut(s) 339, 400, 856, 928
DraI TTTAAA 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 843, 864
EarI CTCTTC 2 cut(s) 843, 864
Eco130I CCWWGG 1 cut(s) 676
Eco32I GATATC 1 cut(s) 638
Eco47I GGWCC 1 cut(s) 485
Eco57I CTGAAG 2 cut(s) 867, 888
EcoO109I RGGNCCY 1 cut(s) 485
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 676
EcoT22I ATGCAT 1 cut(s) 922
ErhI CCWWGG 1 cut(s) 676
FbaI TGATCA 3 cut(s) 339, 856, 928
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 4 cut(s) 586, 684, 729, 774
FokI GGATG 4 cut(s) 139, 527, 679, 929
Fsp4HI GCNGC 4 cut(s) 586, 684, 729, 774
FspBI CTAG 2 cut(s) 582, 677
GlaI GCGC 1 cut(s) 772
GluI GCNGC 4 cut(s) 586, 684, 729, 774
HaeIII GGCC 2 cut(s) 80, 792
HapII CCGG 1 cut(s) 456
HhaI GCGC 1 cut(s) 773
Hin6I GCGC 1 cut(s) 771
HinP1I GCGC 1 cut(s) 771
HindIII AAGCTT 2 cut(s) 108, 301
HinfI GANTC 3 cut(s) 508, 744, 851
HpaII CCGG 1 cut(s) 456
HphI GGTGA 1 cut(s) 833
Hpy166II GTNNAC 3 cut(s) 142, 512, 832
Hpy188I TCNGA 3 cut(s) 847, 856, 861
Hpy188III TCNNGA 3 cut(s) 200, 398, 910
Hpy8I GTNNAC 3 cut(s) 142, 512, 832
Hpy99I CGWCG 1 cut(s) 898
HpyAV CCTTC 5 cut(s) 124, 334, 553, 812, 907
HpyCH4III ACNGT 4 cut(s) 442, 577, 690, 739
HpyCH4V TGCA 9 cut(s) 299, 332, 374, 588, 616, 644, 704, 762, 920
HpyF10VI GCNNNNNNNGC 3 cut(s) 692, 701, 768
HpyF3I CTNAG 2 cut(s) 451, 840
HspAI GCGC 1 cut(s) 771
Ksp22I TGATCA 3 cut(s) 339, 856, 928
Kzo9I GATC 4 cut(s) 339, 400, 856, 928
LmnI GCTCC 1 cut(s) 186
LpnPI CCDG 6 cut(s) 153, 251, 469, 538, 602, 654
Lsp1109I GCAGC 4 cut(s) 572, 695, 715, 785
LweI GCATC 2 cut(s) 341, 907
MaeI CTAG 2 cut(s) 582, 677
MaeIII GTNAC 1 cut(s) 622
MalI GATC 4 cut(s) 341, 402, 858, 930
MboI GATC 4 cut(s) 339, 400, 856, 928
MboII GAAGA 4 cut(s) 395, 860, 881, 894
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 1 cut(s) 567
MlyI GAGTC 2 cut(s) 517, 860
MmeI TCCRAC 3 cut(s) 344, 369, 721
Mph1103I ATGCAT 1 cut(s) 922
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 2 cut(s) 284, 305
MslI CAYNNNNRTG 2 cut(s) 176, 365
MspA1I CMGCKG 1 cut(s) 695
MspCI CTTAAG 1 cut(s) 304
MspI CCGG 1 cut(s) 456
MwoI GCNNNNNNNGC 3 cut(s) 692, 701, 768
NdeII GATC 4 cut(s) 339, 400, 856, 928
NheI GCTAGC 1 cut(s) 581
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 1 cut(s) 622
NsiI ATGCAT 1 cut(s) 922
NspI RCATGY 1 cut(s) 292
NspV TTCGAA 1 cut(s) 406
OliI CACNNNNGTG 1 cut(s) 365
PciI ACATGT 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 1 cut(s) 744
PkrI GCNGC 4 cut(s) 587, 685, 730, 775
PleI GAGTC 2 cut(s) 516, 859
PpsI GAGTC 2 cut(s) 516, 859
PpuMI RGGWCCY 1 cut(s) 485
PscI ACATGT 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 485
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 485
PspPPI RGGWCCY 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 3 cut(s) 293, 479, 608
RsaNI GTAC 3 cut(s) 292, 478, 607
RseI CAYNNNNRTG 2 cut(s) 176, 365
SaqAI TTAA 2 cut(s) 284, 305
SatI GCNGC 4 cut(s) 586, 684, 729, 774
Sau3AI GATC 4 cut(s) 339, 400, 856, 928
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 2 cut(s) 517, 860
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 2 cut(s) 341, 907
SfuI TTCGAA 1 cut(s) 406
SinI GGWCC 1 cut(s) 485
SmiMI CAYNNNNRTG 2 cut(s) 176, 365
SmlI CTYRAG 3 cut(s) 72, 304, 383
SmoI CTYRAG 3 cut(s) 72, 304, 383
Sse9I AATT 1 cut(s) 567
SspMI CTAG 2 cut(s) 582, 677
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 4 cut(s) 442, 577, 690, 739
TaqI TCGA 3 cut(s) 154, 406, 753
TasI AATT 1 cut(s) 567
TatI WGTACW 2 cut(s) 291, 477
TfiI GAWTC 1 cut(s) 744
Tru1I TTAA 2 cut(s) 284, 305
Tru9I TTAA 2 cut(s) 284, 305
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 4 cut(s) 585, 683, 728, 773
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 4 cut(s) 88, 143, 840, 882
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 485
XceI RCATGY 1 cut(s) 292
XmaJI CCTAGG 1 cut(s) 676
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 2 cut(s) 582, 677
Zsp2I ATGCAT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.