Rw4G003300

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0002399
Physical Location & Seq
Forward (+)
6604940 .. 6607595
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G003300.1

Sequence Viewer

Length: 819 bp
ATGTACTTTGCAAGCTTAAGATACACCTCTCCAACCTTCCTTGCATCAATGATCAACACCATAGCTTCCCTCACTTTTGTGATTGCAGTTTCACTCAAGTTGGAGGTTCTTGATCTTCGAAATCCTCATGGATTAGCAAAAGTTTTGGGCACCGCAATCTCCTTAGCCGGTGTAATGACCATGACTTTGTACAAAGGACCTATTATGAGAAACTTGTGGAGTCCACTAATCCATATTCATGGAAAAACTTCCATCCATGAGAACTGGTTGAAGGGTTCAATTCTAACTGTTGCTAGCTGCATAACATGGTCTTTATGGTACATTATGCAGGCAGTCACATTGAAAAGATATCCCGCACAACTATCAATGATAACATGGATGAGCTTCCTAGGGGCAGCACAGTCAGCTGTCTTTGCAGTATGTATAGAACATAAACGAGCTGCTTGGACAGTTGGATTCAACATTGACCTCTGGTGCATTTTGTATTCTGGAGGGGTGTGCTCTGGCCTAATACTCTTTATTCAACTATGGTGCACAGTAGAGAAAGGACCTGTCTTTGTGACCATGTTTGATCCCGTCTCAACAATATTGGTGGCAATTCTAGCATACTTTGTGCTTGGTGAAAGACTCTATATTGGCAGCATAGTAGGCGCAGCTATCGTCATTGTTGGCCTATACTTGCTGTTGTGGGGAAAAGAAGGTGATGAAGTTTACACCACTAAGTCTGAAGAGTCTGATCAGACAAATGAAGAGGAGAAAGATAACAATATGCCGACGATTTCTTCAGTCAAGAAGGATGCATTACATGATCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

272

Amino Acids

30.17

Weight (kDa)

6.58

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 91 - 229 1.6e-12 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AciI CCGC 2 cut(s) 153, 354
AclWI GGATC 1 cut(s) 566
AcuI CTGAAG 2 cut(s) 747, 768
AfaI GTAC 3 cut(s) 5, 191, 320
AflII CTTAAG 1 cut(s) 16
AgsI TTSAA 5 cut(s) 271, 279, 343, 460, 524
AleI CACNNNNGTG 1 cut(s) 77
AluBI AGCT 7 cut(s) 15, 65, 297, 384, 407, 440, 656
AluI AGCT 7 cut(s) 15, 65, 297, 384, 407, 440, 656
Alw21I GWGCWC 2 cut(s) 503, 536
Alw26I GTCTC 1 cut(s) 583
Alw44I GTGCAC 1 cut(s) 532
AlwI GGATC 1 cut(s) 566
AoxI GGCC 2 cut(s) 505, 670
ApaLI GTGCAC 1 cut(s) 532
ApeKI GCWGC 5 cut(s) 297, 395, 440, 639, 653
ArsI GACNNNNNNTTYG 2 cut(s) 169, 201
Asp700I GAANNNNTTC 1 cut(s) 247
AspA2I CCTAGG 1 cut(s) 388
AspLEI GCGC 1 cut(s) 653
AspS9I GGNCC 2 cut(s) 197, 548
AsuHPI GGTGA 2 cut(s) 632, 713
AsuII TTCGAA 1 cut(s) 118
AsuNHI GCTAGC 1 cut(s) 293
AvaII GGWCC 2 cut(s) 197, 548
AvrII CCTAGG 1 cut(s) 388
BaeGI GKGCMC 2 cut(s) 152, 536
BanI GGYRCC 1 cut(s) 149
Bbv12I GWGCWC 2 cut(s) 503, 536
BbvI GCAGC 5 cut(s) 284, 407, 427, 651, 665
BccI CCATC 1 cut(s) 260
BclI TGATCA 3 cut(s) 51, 736, 808
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 3 cut(s) 294, 389, 602
BfrI CTTAAG 1 cut(s) 16
BisI GCNGC 5 cut(s) 298, 396, 441, 640, 654
BlnI CCTAGG 1 cut(s) 388
BlsI GCNGC 5 cut(s) 299, 397, 442, 641, 655
Bme18I GGWCC 2 cut(s) 197, 548
BmgT120I GGNCC 2 cut(s) 197, 548
BmiI GGNNCC 1 cut(s) 151
BmsI GCATC 2 cut(s) 53, 787
BmtI GCTAGC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 163
Bpu14I TTCGAA 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 80
BsaJI CCNNGG 1 cut(s) 388
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 1 cut(s) 269
BseDI CCNNGG 1 cut(s) 388
BseGI GGATG 3 cut(s) 252, 384, 802
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 1 cut(s) 767
BseSI GKGCMC 2 cut(s) 152, 536
BseXI GCAGC 5 cut(s) 284, 407, 427, 651, 665
BshFI GGCC 2 cut(s) 507, 672
BshNI GGYRCC 1 cut(s) 149
BsiHKAI GWGCWC 2 cut(s) 503, 536
BsiSI CCGG 1 cut(s) 168
BsmAI GTCTC 1 cut(s) 583
BsmBI CGTCTC 1 cut(s) 583
BsnI GGCC 2 cut(s) 507, 672
Bsp119I TTCGAA 1 cut(s) 118
Bsp1286I GDGCHC 3 cut(s) 152, 503, 536
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 5 cut(s) 51, 112, 571, 736, 808
BspACI CCGC 2 cut(s) 153, 354
BspANI GGCC 2 cut(s) 507, 672
BspLI GGNNCC 1 cut(s) 151
BspOI GCTAGC 1 cut(s) 297
BspPI GGATC 1 cut(s) 566
BspT104I TTCGAA 1 cut(s) 118
BspT107I GGYRCC 1 cut(s) 149
BspTI CTTAAG 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 167
BsrGI TGTACA 1 cut(s) 189
BsrI ACTGG 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 1 cut(s) 388
BssMI GATC 5 cut(s) 51, 112, 571, 736, 808
BssT1I CCWWGG 1 cut(s) 388
Bst4CI ACNGT 4 cut(s) 289, 402, 451, 538
Bst6I CTCTTC 2 cut(s) 723, 744
BstAFI CTTAAG 1 cut(s) 16
BstAUI TGTACA 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 118
BstC8I GCNNGC 3 cut(s) 13, 295, 330
BstDEI CTNAG 2 cut(s) 163, 720
BstF5I GGATG 3 cut(s) 252, 384, 802
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 5 cut(s) 54, 115, 574, 739, 811
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 5 cut(s) 51, 112, 571, 736, 808
BstMWI GCNNNNNNNGC 4 cut(s) 404, 413, 602, 648
BstSLI GKGCMC 2 cut(s) 152, 536
BstV1I GCAGC 5 cut(s) 284, 407, 427, 651, 665
BstXI CCANNNNNNTGG 1 cut(s) 239
BsuRI GGCC 2 cut(s) 507, 672
BtsCI GGATG 3 cut(s) 252, 384, 802
Cac8I GCNNGC 3 cut(s) 13, 295, 330
CfoI GCGC 1 cut(s) 653
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 197, 548
Csp6I GTAC 3 cut(s) 4, 190, 319
CspCI CAANNNNNGTGG 2 cut(s) 573, 608
CviAII CATG 9 cut(s) 128, 181, 239, 257, 306, 375, 565, 806, 816
CviQI GTAC 3 cut(s) 4, 190, 319
DdeI CTNAG 2 cut(s) 163, 720
DpnI GATC 5 cut(s) 53, 114, 573, 738, 810
DpnII GATC 5 cut(s) 51, 112, 571, 736, 808
Eam1104I CTCTTC 2 cut(s) 723, 744
EarI CTCTTC 2 cut(s) 723, 744
Eco130I CCWWGG 1 cut(s) 388
Eco32I GATATC 1 cut(s) 350
Eco47I GGWCC 2 cut(s) 197, 548
Eco57I CTGAAG 2 cut(s) 747, 768
EcoO109I RGGNCCY 2 cut(s) 197, 548
EcoRV GATATC 1 cut(s) 350
EcoT14I CCWWGG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 802
ErhI CCWWGG 1 cut(s) 388
Esp3I CGTCTC 1 cut(s) 583
FaeI CATG 9 cut(s) 131, 184, 242, 260, 309, 378, 568, 809, 819
FatI CATG 9 cut(s) 127, 180, 238, 256, 305, 374, 564, 805, 815
FauI CCCGC 1 cut(s) 361
FbaI TGATCA 3 cut(s) 51, 736, 808
Fnu4HI GCNGC 5 cut(s) 298, 396, 441, 640, 654
FokI GGATG 3 cut(s) 239, 391, 809
Fsp4HI GCNGC 5 cut(s) 298, 396, 441, 640, 654
FspBI CTAG 3 cut(s) 294, 389, 602
GlaI GCGC 1 cut(s) 652
GluI GCNGC 5 cut(s) 298, 396, 441, 640, 654
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 2 cut(s) 507, 672
HapII CCGG 1 cut(s) 168
HhaI GCGC 1 cut(s) 653
Hin1II CATG 9 cut(s) 131, 184, 242, 260, 309, 378, 568, 809, 819
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 4 cut(s) 220, 456, 627, 731
HpaII CCGG 1 cut(s) 168
HphI GGTGA 2 cut(s) 632, 713
Hpy166II GTNNAC 3 cut(s) 224, 534, 712
Hpy188I TCNGA 3 cut(s) 727, 736, 741
Hpy188III TCNNGA 3 cut(s) 110, 489, 790
Hpy8I GTNNAC 3 cut(s) 224, 534, 712
Hpy99I CGWCG 1 cut(s) 778
HpyAV CCTTC 4 cut(s) 46, 265, 692, 787
HpyCH4III ACNGT 4 cut(s) 289, 402, 451, 538
HpyCH4V TGCA 9 cut(s) 11, 44, 86, 300, 328, 416, 477, 534, 800
HpyF10VI GCNNNNNNNGC 4 cut(s) 404, 413, 602, 648
HpyF3I CTNAG 2 cut(s) 163, 720
Hsp92II CATG 9 cut(s) 131, 184, 242, 260, 309, 378, 568, 809, 819
HspAI GCGC 1 cut(s) 651
Ksp22I TGATCA 3 cut(s) 51, 736, 808
Kzo9I GATC 5 cut(s) 51, 112, 571, 736, 808
LpnPI CCDG 7 cut(s) 181, 250, 314, 457, 474, 489, 564
Lsp1109I GCAGC 5 cut(s) 284, 407, 427, 651, 665
LweI GCATC 2 cut(s) 53, 787
MaeI CTAG 3 cut(s) 294, 389, 602
MaeIII GTNAC 2 cut(s) 334, 559
MalI GATC 5 cut(s) 53, 114, 573, 738, 810
MboI GATC 5 cut(s) 51, 112, 571, 736, 808
MboII GAAGA 4 cut(s) 107, 740, 761, 774
MhlI GDGCHC 3 cut(s) 152, 503, 536
MluCI AATT 2 cut(s) 279, 597
MlyI GAGTC 3 cut(s) 229, 621, 740
MmeI TCCRAC 3 cut(s) 56, 81, 433
MnlI CCTC 7 cut(s) 37, 80, 97, 135, 479, 485, 745
Mph1103I ATGCAT 1 cut(s) 802
MroXI GAANNNNTTC 1 cut(s) 247
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 2 cut(s) 77, 237
MspA1I CMGCKG 1 cut(s) 407
MspCI CTTAAG 1 cut(s) 16
MspI CCGG 1 cut(s) 168
MwoI GCNNNNNNNGC 4 cut(s) 404, 413, 602, 648
NdeII GATC 5 cut(s) 51, 112, 571, 736, 808
NheI GCTAGC 1 cut(s) 293
NlaIII CATG 9 cut(s) 131, 184, 242, 260, 309, 378, 568, 809, 819
NlaIV GGNNCC 1 cut(s) 151
NmuCI GTSAC 2 cut(s) 334, 559
NsiI ATGCAT 1 cut(s) 802
NspV TTCGAA 1 cut(s) 118
OliI CACNNNNGTG 1 cut(s) 77
PdmI GAANNNNTTC 1 cut(s) 247
PfeI GAWTC 1 cut(s) 456
PkrI GCNGC 5 cut(s) 299, 397, 442, 641, 655
PleI GAGTC 3 cut(s) 228, 621, 739
PpsI GAGTC 3 cut(s) 228, 621, 739
PpuMI RGGWCCY 2 cut(s) 197, 548
Psp5II RGGWCCY 2 cut(s) 197, 548
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 2 cut(s) 197, 548
PspPPI RGGWCCY 2 cut(s) 197, 548
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 3 cut(s) 5, 191, 320
RsaNI GTAC 3 cut(s) 4, 190, 319
RseI CAYNNNNRTG 2 cut(s) 77, 237
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 5 cut(s) 298, 396, 441, 640, 654
Sau3AI GATC 5 cut(s) 51, 112, 571, 736, 808
Sau96I GGNCC 2 cut(s) 197, 548
SchI GAGTC 3 cut(s) 229, 621, 740
SduI GDGCHC 3 cut(s) 152, 503, 536
SfaNI GCATC 2 cut(s) 53, 787
SfuI TTCGAA 1 cut(s) 118
SinI GGWCC 2 cut(s) 197, 548
SmiMI CAYNNNNRTG 2 cut(s) 77, 237
SmlI CTYRAG 2 cut(s) 16, 95
SmoI CTYRAG 2 cut(s) 16, 95
Sse9I AATT 2 cut(s) 279, 597
SsiI CCGC 2 cut(s) 153, 354
SspI AATATT 1 cut(s) 588
SspMI CTAG 3 cut(s) 294, 389, 602
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 4 cut(s) 289, 402, 451, 538
TaqI TCGA 1 cut(s) 118
TasI AATT 2 cut(s) 279, 597
TatI WGTACW 2 cut(s) 3, 189
TfiI GAWTC 1 cut(s) 456
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TseFI GTSAC 2 cut(s) 334, 559
TseI GCWGC 5 cut(s) 297, 395, 440, 639, 653
Tsp45I GTSAC 2 cut(s) 334, 559
TspDTI ATGAA 3 cut(s) 227, 720, 762
Vha464I CTTAAG 1 cut(s) 16
VneI GTGCAC 1 cut(s) 532
VpaK11BI GGWCC 2 cut(s) 197, 548
XmaJI CCTAGG 1 cut(s) 388
XmnI GAANNNNTTC 1 cut(s) 247
XspI CTAG 3 cut(s) 294, 389, 602
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.