RLG00000009815

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0002399
Physical Location & Seq
Reverse (-)
58349008 .. 58352356
3349 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009815

Sequence Viewer

Length: 804 bp
ATGGCACAAGAGCCAAGTTCCCATGTGGGAACTTACAAGAGGTTCAAGCCACACCTCCTCATGGTTTTATCTCAAGTGGCCTACGCATTTCTCTATTTCATTACAGAAGCTTCCTTCAATCATGGGATGAACCCTCATGTCTACATAACTTATCGACATATCGTGTCTGGCATAATCATGCTCCCTTGTGCCTATTTTCTTGAAAGAAAAGACAGACCAAAGCTCACATTTGCTCTTTTCTTGGAACTTTTTGTTATTTCTTTTCTGGGGATAGGTTTGACTTTAAACATGTACTTTGCAAGCTTAAGATACACCTCGCCAACCTTCCTTGCATCAATGATCAACACCATAGCTTCCCTCACTTTTGTGATTGCAGTTTCACTCAAATTGGAGGTTCTTGATCTTCGAAATCCTCGTGGATTTGCAAAAGTTTTGGGCACCATAGTCTCCTTAGCCGGTGTAATGACCATGACTTTGTACAAAGGACCTATTATGAGCAACTTGTGGAGTCCACTAATCCATATTCAAGGAAAAACTTCCATCCATGAGAACTGGTTGAAGGGTTCAATTCTAACTGTTGCTAGCTGCATAACATGGTCTTTATGGTACATTATGCAGGCAGTCACATTGAAAAGATATCCTGCACAACTATCAATGATAACATGGATGAGCTTCCTAGGGGCAGCACAGTCAGCTGTCTTTGCAGTTTGTATAGAACATAAACGAGCTGCTTGGACAGTTGGATTCAACATTGACCTCTGGTGCATTTTGTATTCTGTAAGTTTCAGGATTCTGAAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

268

Amino Acids

30.29

Weight (kDa)

9.67

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 18 - 158 4.6e-10 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 437
AccI GTMKAC 1 cut(s) 141
AfaI GTAC 3 cut(s) 293, 479, 608
AfiI CCNNNNNNNGG 1 cut(s) 61
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 8 cut(s) 46, 118, 203, 527, 559, 567, 631, 748
AleI CACNNNNGTG 1 cut(s) 365
AluBI AGCT 8 cut(s) 110, 223, 303, 353, 585, 672, 695, 728
AluI AGCT 8 cut(s) 110, 223, 303, 353, 585, 672, 695, 728
Alw26I GTCTC 1 cut(s) 451
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 3 cut(s) 585, 683, 728
ArsI GACNNNNNNTTYG 2 cut(s) 457, 489
Asp700I GAANNNNTTC 1 cut(s) 535
AspA2I CCTAGG 1 cut(s) 676
AspS9I GGNCC 1 cut(s) 485
AsuII TTCGAA 1 cut(s) 406
AsuNHI GCTAGC 1 cut(s) 581
AvaII GGWCC 1 cut(s) 485
AvrII CCTAGG 1 cut(s) 676
BaeGI GKGCMC 1 cut(s) 440
BanI GGYRCC 1 cut(s) 437
BauI CACGAG 1 cut(s) 414
BbvI GCAGC 3 cut(s) 572, 695, 715
BccI CCATC 1 cut(s) 548
BclI TGATCA 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 2 cut(s) 582, 677
BfrI CTTAAG 1 cut(s) 304
BisI GCNGC 3 cut(s) 586, 684, 729
BlnI CCTAGG 1 cut(s) 676
BlsI GCNGC 3 cut(s) 587, 685, 730
Bme18I GGWCC 1 cut(s) 485
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 439
BmsI GCATC 1 cut(s) 341
BmtI GCTAGC 1 cut(s) 585
Bpu10I CCTNAGC 1 cut(s) 451
Bpu14I TTCGAA 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 57
BsaJI CCNNGG 1 cut(s) 676
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 455
Bse1I ACTGG 1 cut(s) 557
BseDI CCNNGG 1 cut(s) 676
BseGI GGATG 3 cut(s) 132, 540, 672
BseLI CCNNNNNNNGG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 557
BseRI GAGGAG 1 cut(s) 47
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 3 cut(s) 572, 695, 715
BsgI GTGCAG 1 cut(s) 627
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 456
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 451
BsnI GGCC 1 cut(s) 80
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 440
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 2 cut(s) 339, 400
BspANI GGCC 1 cut(s) 80
BspLI GGNNCC 1 cut(s) 439
BspOI GCTAGC 1 cut(s) 585
BspT104I TTCGAA 1 cut(s) 406
BspT107I GGYRCC 1 cut(s) 437
BspTI CTTAAG 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 455
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 1 cut(s) 557
BssAI RCCGGY 1 cut(s) 455
BssECI CCNNGG 1 cut(s) 676
BssMI GATC 2 cut(s) 339, 400
BssSI CACGAG 1 cut(s) 414
BssT1I CCWWGG 1 cut(s) 676
Bst2BI CACGAG 1 cut(s) 414
Bst4CI ACNGT 3 cut(s) 577, 690, 739
BstAFI CTTAAG 1 cut(s) 304
BstAUI TGTACA 1 cut(s) 477
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 3 cut(s) 301, 583, 618
BstDEI CTNAG 1 cut(s) 451
BstF5I GGATG 3 cut(s) 132, 540, 672
BstKTI GATC 2 cut(s) 342, 403
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 2 cut(s) 339, 400
BstMWI GCNNNNNNNGC 2 cut(s) 692, 701
BstNSI RCATGY 1 cut(s) 292
BstSLI GKGCMC 1 cut(s) 440
BstV1I GCAGC 3 cut(s) 572, 695, 715
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 3 cut(s) 132, 540, 672
Cac8I GCNNGC 3 cut(s) 301, 583, 618
Cfr10I RCCGGY 1 cut(s) 455
Cfr13I GGNCC 1 cut(s) 485
Csp6I GTAC 3 cut(s) 292, 478, 607
CviQI GTAC 3 cut(s) 292, 478, 607
DdeI CTNAG 1 cut(s) 451
DpnI GATC 2 cut(s) 341, 402
DpnII GATC 2 cut(s) 339, 400
DraI TTTAAA 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 676
Eco32I GATATC 1 cut(s) 638
Eco47I GGWCC 1 cut(s) 485
EcoO109I RGGNCCY 1 cut(s) 485
EcoRV GATATC 1 cut(s) 638
EcoT14I CCWWGG 1 cut(s) 676
ErhI CCWWGG 1 cut(s) 676
FbaI TGATCA 1 cut(s) 339
FblI GTMKAC 1 cut(s) 141
Fnu4HI GCNGC 3 cut(s) 586, 684, 729
FokI GGATG 3 cut(s) 139, 527, 679
Fsp4HI GCNGC 3 cut(s) 586, 684, 729
FspBI CTAG 2 cut(s) 582, 677
GluI GCNGC 3 cut(s) 586, 684, 729
HaeIII GGCC 1 cut(s) 80
HapII CCGG 1 cut(s) 456
HindIII AAGCTT 2 cut(s) 108, 301
HinfI GANTC 3 cut(s) 508, 744, 790
HpaII CCGG 1 cut(s) 456
Hpy166II GTNNAC 2 cut(s) 142, 512
Hpy188I TCNGA 1 cut(s) 795
Hpy188III TCNNGA 3 cut(s) 200, 398, 787
Hpy8I GTNNAC 2 cut(s) 142, 512
HpyAV CCTTC 3 cut(s) 124, 334, 553
HpyCH4III ACNGT 3 cut(s) 577, 690, 739
HpyCH4V TGCA 9 cut(s) 299, 332, 374, 425, 588, 616, 644, 704, 765
HpyF10VI GCNNNNNNNGC 2 cut(s) 692, 701
HpyF3I CTNAG 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 339
Kzo9I GATC 2 cut(s) 339, 400
LmnI GCTCC 1 cut(s) 186
LpnPI CCDG 8 cut(s) 153, 251, 469, 538, 602, 654, 745, 772
Lsp1109I GCAGC 3 cut(s) 572, 695, 715
LweI GCATC 1 cut(s) 341
MaeI CTAG 2 cut(s) 582, 677
MaeIII GTNAC 1 cut(s) 622
MalI GATC 2 cut(s) 341, 402
MboI GATC 2 cut(s) 339, 400
MboII GAAGA 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 2 cut(s) 386, 567
MlyI GAGTC 1 cut(s) 517
MmeI TCCRAC 1 cut(s) 721
MnlI CCTC 9 cut(s) 33, 65, 68, 144, 325, 368, 385, 423, 767
MroXI GAANNNNTTC 1 cut(s) 535
MseI TTAA 2 cut(s) 284, 305
MslI CAYNNNNRTG 2 cut(s) 176, 365
MspA1I CMGCKG 1 cut(s) 695
MspCI CTTAAG 1 cut(s) 304
MspI CCGG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 692, 701
NdeII GATC 2 cut(s) 339, 400
NheI GCTAGC 1 cut(s) 581
NlaIV GGNNCC 1 cut(s) 439
NmuCI GTSAC 1 cut(s) 622
NspI RCATGY 1 cut(s) 292
NspV TTCGAA 1 cut(s) 406
OliI CACNNNNGTG 1 cut(s) 365
PciI ACATGT 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 412
PdmI GAANNNNTTC 1 cut(s) 535
PfeI GAWTC 2 cut(s) 744, 790
PkrI GCNGC 3 cut(s) 587, 685, 730
PleI GAGTC 1 cut(s) 516
PpsI GAGTC 1 cut(s) 516
PpuMI RGGWCCY 1 cut(s) 485
PscI ACATGT 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 485
PspN4I GGNNCC 1 cut(s) 439
PspPI GGNCC 1 cut(s) 485
PspPPI RGGWCCY 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 3 cut(s) 293, 479, 608
RsaNI GTAC 3 cut(s) 292, 478, 607
RseI CAYNNNNRTG 2 cut(s) 176, 365
SaqAI TTAA 2 cut(s) 284, 305
SatI GCNGC 3 cut(s) 586, 684, 729
Sau3AI GATC 2 cut(s) 339, 400
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 1 cut(s) 517
SduI GDGCHC 1 cut(s) 440
SfaNI GCATC 1 cut(s) 341
SfuI TTCGAA 1 cut(s) 406
SinI GGWCC 1 cut(s) 485
SmiMI CAYNNNNRTG 2 cut(s) 176, 365
SmlI CTYRAG 2 cut(s) 72, 304
SmoI CTYRAG 2 cut(s) 72, 304
Sse9I AATT 2 cut(s) 386, 567
SspMI CTAG 2 cut(s) 582, 677
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 3 cut(s) 577, 690, 739
TaqI TCGA 2 cut(s) 154, 406
TasI AATT 2 cut(s) 386, 567
TatI WGTACW 2 cut(s) 291, 477
TfiI GAWTC 2 cut(s) 744, 790
Tru1I TTAA 2 cut(s) 284, 305
Tru9I TTAA 2 cut(s) 284, 305
TseFI GTSAC 1 cut(s) 622
TseI GCWGC 3 cut(s) 585, 683, 728
Tsp45I GTSAC 1 cut(s) 622
TspDTI ATGAA 2 cut(s) 88, 143
Vha464I CTTAAG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 485
XceI RCATGY 1 cut(s) 292
XmaJI CCTAGG 1 cut(s) 676
XmiI GTMKAC 1 cut(s) 141
XmnI GAANNNNTTC 1 cut(s) 535
XspI CTAG 2 cut(s) 582, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.