RLG00000012404

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0002399
Physical Location & Seq
Forward (+)
18467464 .. 18470226
2763 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012404

Sequence Viewer

Length: 1392 bp
ATGGCTCTTGTATTGAGTCCATACATGAAGATCTACAGAAGATTCAAGCCACACCTTCTCATGGTTTTGGCACAGATCGGTGCTGTATTTCTCTACTTCATTACCGATGCATCCTTCAATCATGGGATGAACCCTCATGTCTTTGTAACCTATCGACATATAGTTGGTGGCTTAGTCATGTTTCCATTTGCCTATTTTCTTGAAAGAAAAGCAAGGCCAAAGTTGACTCTAGCATTGTTTATGGAGATATTTGTGATTTCCCTTCTAGGGGTTAGCTTAACCCTTAATATGTATTTTGCAAGCCTGAAGTACACATCTTCAAGCTTCGTCACATCAATAGCCAATACCATACCATCCCTGACTTTTCTGATTGCAGTCATACTCAGGATGGAGGCTGTAGATGTAAGGAATCCGCGCGGAATAGCTAAACTCTTGGGAACCTTAATATCTCTGGCTGGGGTCATCACCATGACTTCATACAAAGGACCGGCAATACAAAGCTTCTCGGGAGCATCACAACACATGAGAAGTAATTTTGTTCACAAAAGCTGGACAAAGGGTTCAATCCTTGTGATTGCAAGTTGTATAACATGGTCCATATGGTACATAATGCAGGGGATCACATTGAAGAAATATCCTGCACAACTGTCACTAACTACATGGATCAATTGTATCGGTGCAGCACAATCCGCTGTGTTCACAGTTATCATACAGCAAAAACGGGCAATATGGAGAATTACATACAACAATGATTTCTGGTCCATCATATATGCTGGAGTTGTATGCTCAGGTATAACAATCTTCATTCAACTATGGTGTACAAAACAAAAAGGACCTGTCTTCGTGACCATGTTCAGTCCTCTTGCAACAACATTAGTAGCGATTCTAGCTTACTTCATTCTCGGCGAAAAGCTGCACATTGGCAGAATAGTGGGAGCGGTCATTATTATTATCGGTCTATACTTAGTCTTATGGGGCAAAGATAGAGATCAGAATCATTTCAAATCCCAAGAGGAACCTACCGTAATTAGTGATAACCAGAAGGAACCCAAGATGCAAGTGGGATCTTCAGCAGAAAGACAGAGCGACCTCCATAACATGAAGGCCGTCGCCTGCCAAATCAAATCCGAGAGGAACGCCGACGTGCGAGAGGTCTACAACAAGTCGTTGAAGCTGGACGACGAGCTCCGCGACCTAGACGCCATGAACGCCGAGTTGACTCTGGTCCGAAACGACATCGAGGACTTGAGCACGTCGCGGATGGAGATCGCGACCGAGTTGAAGATGATCCAAAGAGAGATTGAGAGGGCTCGCTCCGATGAGTCGAAGCAAATCGAAGCCGTTAGAGACGAAATTGAGACTTTGAAGCTGGAGATTGAGAAAGGAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

464

Amino Acids

52.37

Weight (kDa)

9.34

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 18 - 157 7.9e-12 EamA-like transporter family
EamA PF00892 186 - 324 6.5e-16 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 938
AccI GTMKAC 1 cut(s) 1155
AccII CGCG 5 cut(s) 415, 417, 1191, 1258, 1271
AciI CCGC 6 cut(s) 413, 417, 690, 938, 1189, 1258
AclWI GGATC 4 cut(s) 626, 671, 1072, 1282
AcuI CTGAAG 2 cut(s) 326, 1053
AcyI GRCGYC 1 cut(s) 1200
AfaI GTAC 3 cut(s) 311, 605, 820
AfiI CCNNNNNNNGG 3 cut(s) 61, 267, 268
AjiI CACGTC 2 cut(s) 1144, 1254
AloI GAACNNNNNNTCC 2 cut(s) 544, 576
Alw21I GWGCWC 2 cut(s) 1188, 1253
Alw26I GTCTC 2 cut(s) 1341, 1352
AlwI GGATC 4 cut(s) 626, 671, 1072, 1282
Ama87I CYCGRG 1 cut(s) 505
AoxI GGCC 2 cut(s) 215, 1104
ApeKI GCWGC 2 cut(s) 680, 913
Asp700I GAANNNNTTC 1 cut(s) 998
AspLEI GCGC 1 cut(s) 417
AspS9I GGNCC 5 cut(s) 485, 594, 759, 833, 1225
AsuHPI GGTGA 1 cut(s) 457
AvaI CYCGRG 1 cut(s) 505
AvaII GGWCC 5 cut(s) 485, 594, 759, 833, 1225
BanII GRGCYC 2 cut(s) 1188, 1312
BbsI GAAGAC 1 cut(s) 832
Bbv12I GWGCWC 2 cut(s) 1188, 1253
BbvI GCAGC 2 cut(s) 692, 900
BccI CCATC 4 cut(s) 361, 382, 770, 1255
BceAI ACGGC 2 cut(s) 1091, 1325
BcoDI GTCTC 2 cut(s) 1341, 1352
BfaI CTAG 4 cut(s) 230, 266, 887, 1196
BfmI CTRYAG 2 cut(s) 34, 396
BglII AGATCT 1 cut(s) 30
BisI GCNGC 2 cut(s) 681, 914
BlsI GCNGC 2 cut(s) 682, 915
Bme18I GGWCC 5 cut(s) 485, 594, 759, 833, 1225
BmeT110I CYCGRG 1 cut(s) 505
BmgBI CACGTC 2 cut(s) 1144, 1254
BmgT120I GGNCC 5 cut(s) 485, 594, 759, 833, 1225
BmiI GGNNCC 3 cut(s) 439, 1017, 1047
BmsI GCATC 4 cut(s) 97, 119, 521, 1044
BoxI GACNNNNGTC 1 cut(s) 1223
BpiI GAAGAC 1 cut(s) 832
BpmI CTGGAG 2 cut(s) 795, 1391
Bpu10I CCTNAGC 1 cut(s) 787
BpuEI CTTGAG 1 cut(s) 1267
BsaBI GATNNNNATC 3 cut(s) 987, 993, 1265
BsaHI GRCGYC 1 cut(s) 1200
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 267, 268
Bse118I RCCGGY 1 cut(s) 487
Bse8I GATNNNNATC 3 cut(s) 987, 993, 1265
BseGI GGATG 5 cut(s) 110, 132, 353, 393, 1266
BseJI GATNNNNATC 3 cut(s) 987, 993, 1265
BseLI CCNNNNNNNGG 3 cut(s) 61, 267, 268
BseMII CTCAG 2 cut(s) 397, 801
BseXI GCAGC 2 cut(s) 692, 900
BseYI CCCAGC 1 cut(s) 455
BsgI GTGCAG 3 cut(s) 624, 699, 899
Bsh1236I CGCG 5 cut(s) 415, 417, 1191, 1258, 1271
Bsh1285I CGRYCG 1 cut(s) 1275
BshFI GGCC 2 cut(s) 217, 1106
BsiEI CGRYCG 1 cut(s) 1275
BsiHKAI GWGCWC 2 cut(s) 1188, 1253
BsiHKCI CYCGRG 1 cut(s) 505
BsiSI CCGG 1 cut(s) 488
BslI CCNNNNNNNGG 3 cut(s) 61, 267, 268
BsmAI GTCTC 2 cut(s) 1341, 1352
BsmBI CGTCTC 1 cut(s) 1341
BsnI GGCC 2 cut(s) 217, 1106
BsoBI CYCGRG 1 cut(s) 505
Bsp1286I GDGCHC 3 cut(s) 1188, 1253, 1312
Bsp1407I TGTACA 1 cut(s) 818
Bsp143I GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
Bsp68I TCGCGA 1 cut(s) 1271
BspACI CCGC 6 cut(s) 413, 417, 690, 938, 1189, 1258
BspANI GGCC 2 cut(s) 217, 1106
BspCNI CTCAG 2 cut(s) 396, 800
BspFNI CGCG 5 cut(s) 415, 417, 1191, 1258, 1271
BspLI GGNNCC 3 cut(s) 439, 1017, 1047
BspPI GGATC 4 cut(s) 626, 671, 1072, 1282
BsrBI CCGCTC 1 cut(s) 938
BsrFI RCCGGY 1 cut(s) 487
BsrGI TGTACA 1 cut(s) 818
BssAI RCCGGY 1 cut(s) 487
BssMI GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
BssNI GRCGYC 1 cut(s) 1200
Bst4CI ACNGT 3 cut(s) 648, 703, 1024
BstACI GRCGYC 1 cut(s) 1200
BstAUI TGTACA 1 cut(s) 818
BstC8I GCNNGC 3 cut(s) 301, 1114, 1312
BstDEI CTNAG 4 cut(s) 172, 383, 787, 964
BstF5I GGATG 5 cut(s) 110, 132, 353, 393, 1266
BstFNI CGCG 5 cut(s) 415, 417, 1191, 1258, 1271
BstHHI GCGC 1 cut(s) 417
BstKTI GATC 8 cut(s) 33, 78, 621, 666, 991, 1067, 1269, 1290
BstMAI GTCTC 2 cut(s) 1341, 1352
BstMBI GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
BstMCI CGRYCG 1 cut(s) 1275
BstMWI GCNNNNNNNGC 3 cut(s) 689, 887, 1208
BstPAI GACNNNNGTC 1 cut(s) 1223
BstSFI CTRYAG 2 cut(s) 34, 396
BstUI CGCG 5 cut(s) 415, 417, 1191, 1258, 1271
BstV1I GCAGC 2 cut(s) 692, 900
BstV2I GAAGAC 1 cut(s) 832
BstX2I RGATCY 2 cut(s) 30, 1064
BstYI RGATCY 2 cut(s) 30, 1064
BsuRI GGCC 2 cut(s) 217, 1106
BtrI CACGTC 2 cut(s) 1144, 1254
BtsCI GGATG 5 cut(s) 110, 132, 353, 393, 1266
BtuMI TCGCGA 1 cut(s) 1271
Cac8I GCNNGC 3 cut(s) 301, 1114, 1312
CfoI GCGC 1 cut(s) 417
Cfr10I RCCGGY 1 cut(s) 487
Cfr13I GGNCC 5 cut(s) 485, 594, 759, 833, 1225
CseI GACGC 1 cut(s) 1208
Csp6I GTAC 3 cut(s) 310, 604, 819
CviQI GTAC 3 cut(s) 310, 604, 819
DdeI CTNAG 4 cut(s) 172, 383, 787, 964
DpnI GATC 8 cut(s) 32, 77, 620, 665, 990, 1066, 1268, 1289
DpnII GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
Ecl136II GAGCTC 1 cut(s) 1186
Eco24I GRGCYC 2 cut(s) 1188, 1312
Eco47I GGWCC 5 cut(s) 485, 594, 759, 833, 1225
Eco53kI GAGCTC 1 cut(s) 1186
Eco57I CTGAAG 2 cut(s) 326, 1053
Eco88I CYCGRG 1 cut(s) 505
EcoICRI GAGCTC 1 cut(s) 1186
EcoO109I RGGNCCY 1 cut(s) 833
EcoT22I ATGCAT 1 cut(s) 112
EcoT38I GRGCYC 2 cut(s) 1188, 1312
Esp3I CGTCTC 1 cut(s) 1341
FauNDI CATATG 1 cut(s) 599
FblI GTMKAC 1 cut(s) 1155
Fnu4HI GCNGC 2 cut(s) 681, 914
FokI GGATG 5 cut(s) 97, 139, 340, 400, 1273
FriOI GRGCYC 2 cut(s) 1188, 1312
Fsp4HI GCNGC 2 cut(s) 681, 914
FspBI CTAG 4 cut(s) 230, 266, 887, 1196
GlaI GCGC 1 cut(s) 416
GluI GCNGC 2 cut(s) 681, 914
GsaI CCCAGC 1 cut(s) 459
GsuI CTGGAG 2 cut(s) 795, 1391
HaeIII GGCC 2 cut(s) 217, 1106
HapII CCGG 1 cut(s) 488
HgaI GACGC 1 cut(s) 1208
HhaI GCGC 1 cut(s) 417
Hin1I GRCGYC 1 cut(s) 1200
Hin6I GCGC 1 cut(s) 415
HinP1I GCGC 1 cut(s) 415
HincII GTYRAC 2 cut(s) 225, 1218
HindII GTYRAC 2 cut(s) 225, 1218
HindIII AAGCTT 2 cut(s) 322, 499
HinfI GANTC 8 cut(s) 16, 42, 226, 409, 883, 994, 1219, 1322
HpaII CCGG 1 cut(s) 488
HphI GGTGA 1 cut(s) 457
Hpy166II GTNNAC 7 cut(s) 225, 312, 541, 699, 819, 1156, 1218
Hpy188I TCNGA 5 cut(s) 369, 993, 1129, 1229, 1318
Hpy188III TCNNGA 5 cut(s) 200, 385, 507, 844, 1270
Hpy8I GTNNAC 7 cut(s) 225, 312, 541, 699, 819, 1156, 1218
Hpy99I CGWCG 4 cut(s) 1112, 1145, 1184, 1258
HpyAV CCTTC 6 cut(s) 65, 124, 272, 1036, 1096, 1380
HpyCH4III ACNGT 3 cut(s) 648, 703, 1024
HpyCH4IV ACGT 2 cut(s) 1143, 1253
HpyF10VI GCNNNNNNNGC 3 cut(s) 689, 887, 1208
HpyF3I CTNAG 4 cut(s) 172, 383, 787, 964
HpySE526I ACGT 2 cut(s) 1143, 1253
Hsp92I GRCGYC 1 cut(s) 1200
HspAI GCGC 1 cut(s) 415
Kzo9I GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
LmnI GCTCC 4 cut(s) 509, 935, 1191, 1319
Lsp1109I GCAGC 2 cut(s) 692, 900
LweI GCATC 4 cut(s) 97, 119, 521, 1044
MaeI CTAG 4 cut(s) 230, 266, 887, 1196
MaeII ACGT 2 cut(s) 1143, 1253
MaeIII GTNAC 4 cut(s) 145, 328, 648, 844
MalI GATC 8 cut(s) 32, 77, 620, 665, 990, 1066, 1268, 1289
MbiI CCGCTC 1 cut(s) 938
MboI GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
MboII GAAGA 8 cut(s) 40, 51, 309, 640, 793, 832, 1059, 1294
MfeI CAATTG 1 cut(s) 667
MflI RGATCY 2 cut(s) 30, 1064
MhlI GDGCHC 3 cut(s) 1188, 1253, 1312
MluCI AATT 5 cut(s) 532, 667, 735, 1026, 1353
MlyI GAGTC 4 cut(s) 25, 220, 1213, 1331
MnlI CCTC 9 cut(s) 144, 385, 870, 1006, 1100, 1125, 1144, 1234, 1299
Mph1103I ATGCAT 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 998
MseI TTAA 3 cut(s) 278, 285, 443
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 1 cut(s) 488
MunI CAATTG 1 cut(s) 667
MvnI CGCG 5 cut(s) 415, 417, 1191, 1258, 1271
MwoI GCNNNNNNNGC 3 cut(s) 689, 887, 1208
NdeI CATATG 1 cut(s) 599
NdeII GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
NlaIV GGNNCC 3 cut(s) 439, 1017, 1047
NmeAIII GCCGAG 2 cut(s) 882, 1237
NmuCI GTSAC 3 cut(s) 328, 648, 844
NruI TCGCGA 1 cut(s) 1271
NsiI ATGCAT 1 cut(s) 112
PcsI WCGNNNNNNNCGW 1 cut(s) 1188
PdmI GAANNNNTTC 1 cut(s) 998
PfeI GAWTC 4 cut(s) 42, 409, 883, 994
PkrI GCNGC 2 cut(s) 682, 915
PleI GAGTC 4 cut(s) 24, 220, 1213, 1330
PpsI GAGTC 4 cut(s) 24, 220, 1213, 1330
PpuMI RGGWCCY 1 cut(s) 833
PshAI GACNNNNGTC 1 cut(s) 1223
Psp124BI GAGCTC 1 cut(s) 1188
Psp5II RGGWCCY 1 cut(s) 833
PspFI CCCAGC 1 cut(s) 455
PspN4I GGNNCC 3 cut(s) 439, 1017, 1047
PspPI GGNCC 5 cut(s) 485, 594, 759, 833, 1225
PspPPI RGGWCCY 1 cut(s) 833
PsuI RGATCY 2 cut(s) 30, 1064
RruI TCGCGA 1 cut(s) 1271
RsaI GTAC 3 cut(s) 311, 605, 820
RsaNI GTAC 3 cut(s) 310, 604, 819
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 1188
SaqAI TTAA 3 cut(s) 278, 285, 443
SatI GCNGC 2 cut(s) 681, 914
Sau3AI GATC 8 cut(s) 30, 75, 618, 663, 988, 1064, 1266, 1287
Sau96I GGNCC 5 cut(s) 485, 594, 759, 833, 1225
SchI GAGTC 4 cut(s) 25, 220, 1213, 1331
SduI GDGCHC 3 cut(s) 1188, 1253, 1312
SfaNI GCATC 4 cut(s) 97, 119, 521, 1044
SfcI CTRYAG 2 cut(s) 34, 396
SinI GGWCC 5 cut(s) 485, 594, 759, 833, 1225
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 1 cut(s) 1246
SmoI CTYRAG 1 cut(s) 1246
Sse9I AATT 5 cut(s) 532, 667, 735, 1026, 1353
SsiI CCGC 6 cut(s) 413, 417, 690, 938, 1189, 1258
SspMI CTAG 4 cut(s) 230, 266, 887, 1196
SstI GAGCTC 1 cut(s) 1188
TaaI ACNGT 3 cut(s) 648, 703, 1024
TaiI ACGT 2 cut(s) 1146, 1256
TaqI TCGA 4 cut(s) 154, 1239, 1325, 1335
TaqII GACCGA 2 cut(s) 944, 1289
TasI AATT 5 cut(s) 532, 667, 735, 1026, 1353
TatI WGTACW 2 cut(s) 309, 818
TfiI GAWTC 4 cut(s) 42, 409, 883, 994
Tru1I TTAA 3 cut(s) 278, 285, 443
Tru9I TTAA 3 cut(s) 278, 285, 443
TseFI GTSAC 3 cut(s) 328, 648, 844
TseI GCWGC 2 cut(s) 680, 913
Tsp45I GTSAC 3 cut(s) 328, 648, 844
TspDTI ATGAA 8 cut(s) 41, 88, 143, 465, 793, 886, 1115, 1220
VpaK11BI GGWCC 5 cut(s) 485, 594, 759, 833, 1225
XcmI CCANNNNNNNNNTGG 1 cut(s) 1057
XmiI GTMKAC 1 cut(s) 1155
XmnI GAANNNNTTC 1 cut(s) 998
XspI CTAG 4 cut(s) 230, 266, 887, 1196
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.