Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002399

Physical Location & Seq

Genomic Coordinates Forward (+)

19806770 .. 19809405

Gene Structure 2636 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1212300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1188 bp

ATGATGTGCTCCTCCAGCTTGTCTAGAACTGAAATAGAAGAAGGAAGAAAGGCGGCCGGACAACCGGAGGAGGTTATGGCACACGAGTTACGTAGTAGTAACCTTAAGGGACTTTACAGGAAGTTCAAGCCACACCTCCTCATGATTTTAGCCCAGATGGGGTATACATTTCTGTATTTTATCACAGAAGCGTCCTTCAATCATGGGATGAACCCTCACGTCTACATAACTTATCGACATATTGTGTCCGGCGTAGTGATGCTCCCTTTTGCTTATTTTCTTGAAAGAACAGAGAGACCGAAGCTCACTTTTGCACTTTTCTTGGAACTTTTTTTACTCTCTCTATTGGGGGTGGGCTTGACATTAAACATGTACTTTGCAAGCTTAAGATACACCTCTCCGACCTTCCTTGCATCAATGGTCAACACCATAGCGTCCCTAACCTTTGTAATTGCAATTGTACTGAGGTTGGAGGCTCTTGACCTTCGAAATCCTAGAGGGTTAGCAAAAGTTTTGGGTAGCCTAATGTCCTTAGCGGGTGTGATGACCATGATATTGTACAAGGGGCCTATTATCAAAAATTTGTGGCCTGCACTAATCCATGTTGAAGGCAAATCCTCCATCCATGAGAACTGGTTAAAGGGTTCAATTCTAACTGTTGCAAGTTGCATAACATGGTCGGCATGGTACATCATGCAGGCAATCACATTGAGAAGATATCCTGCACAACTATCATTGACTACATGGATGAGCTTTATAGGGGCAGCACAATCAGCTGTCTTCACCGTGTGTATAGAACATAGACGAGCTGCTTGGACAATCGGATTCAACGTTGACCTCTGGTCGATATTATATGCTGGAGTGGTGTGCTCAGGTCTAATAATCTTCATTCAACTATGGTGCACAGAAGAGAAAGGGCCAGTTTTTGTGACCATGTTTAATCCTGTATCAACAATTTTGGTGGCTGTTTTAGCTTACTTTGTCCTCGGTGAAAGACTTTATGCTGGCAGCATAGTAGGGGCGTTCATTGTCATCCTTGGTCTATACTTGCTGTTATGGGGAAAAGAAGGTGATGAAATTTACGTCAAGGCGGAAGAGTCATCTTATCAGACAAAGTATGAAGAGCATAAAGATAATGGCGTACAGATCACTTCAGCTAACAAAAATGTGCAGCATGGTGAACCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

396

Amino Acids

44.37

Weight (kDa)

8.66

Isoelectric Point (pI)

41.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	45 - 184	1.5e-08	EamA-like transporter family
EamA	PF00892	214 - 352	1.7e-14	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002399)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g21770 FvH4_4g03390 FvH4_4g03390
malus_domestica	MD10G1186000.v1.1 MD13G1212300.v1.1 MD16G1215900.v1.1
prunus_persica	Prupe.1G037200_v2.0.a1 Prupe.8G225600_v2.0.a1 Prupe.8G225600_v2.0.a1 Prupe.8G225600_v2.0.a1
pyrus_communis	pycom10g16090
rosa_chinensis	RchiOBHm_Chr4g0392371 RchiOBHm_Chr4g0392391 RchiOBHm_Chr6g0288411 RchiOBHm_Chr6g0288431
rosa_laevigata	RLG00000009815 RLG00000009816 RLG00000012404
rosa_multiflora	Rmu_sc0001038.1_g000027 Rmu_sc0001800.1_g000018
rosa_roxburghii	Rroxscaffold_5G00337550 Rroxscaffold_5G00337570 Rroxscaffold_7G00180070 Rroxscaffold_7G00180080
rosa_rugosa	Rorug06G0196300 Rorug06G0196400
rosa_samantha	Rh4AG042200 Rh4AG042300 Rh4BG037100 Rh4BG037200 Rh4CG045000 Rh4CG045100 Rh4DG039200 Rh4DG039600 Rh6AG307900 Rh6BG313300 Rh6CG321100 Rh6DG306500
rosa_wichuraiana	Rw4G003290 Rw4G003300 Rw6G026590 Rw6G026610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	164, 222
AciI	CCGC	3 cut(s)	53, 536, 1091
AclI	AACGTT	1 cut(s)	831
AcoI	YGGCCR	1 cut(s)	54
AcsI	RAATTY	2 cut(s)	580, 1077
AcuI	CTGAAG	1 cut(s)	1137
AdeI	CACNNNGTG	1 cut(s)	789
AfaI	GTAC	5 cut(s)	374, 462, 560, 689, 1143
AfiI	CCNNNNNNNGG	1 cut(s)	159
AflII	CTTAAG	2 cut(s)	104, 385
AflIII	ACRYGT	1 cut(s)	369
AgsI	TTSAA	7 cut(s)	127, 199, 284, 608, 648, 829, 893
AhdI	GACNNNNNGTC	1 cut(s)	841
AjiI	CACGTC	1 cut(s)	220
AluBI	AGCT	8 cut(s)	18, 304, 384, 753, 776, 809, 974, 1157
AluI	AGCT	8 cut(s)	18, 304, 384, 753, 776, 809, 974, 1157
Alw21I	GWGCWC	3 cut(s)	11, 872, 905
Alw26I	GTCTC	1 cut(s)	289
Alw44I	GTGCAC	1 cut(s)	901
AoxI	GGCC	4 cut(s)	54, 566, 587, 917
ApaLI	GTGCAC	1 cut(s)	901
ApeKI	GCWGC	4 cut(s)	764, 809, 1008, 1171
ApoI	RAATTY	2 cut(s)	580, 1077
AspS9I	GGNCC	2 cut(s)	566, 917
AsuHPI	GGTGA	3 cut(s)	775, 1001, 1082
AsuII	TTCGAA	1 cut(s)	487
BaeGI	GKGCMC	1 cut(s)	905
BauI	CACGAG	1 cut(s)	83
BbsI	GAAGAC	1 cut(s)	772
Bbv12I	GWGCWC	3 cut(s)	11, 872, 905
BbvI	GCAGC	4 cut(s)	776, 796, 1020, 1183
BccI	CCATC	2 cut(s)	151, 629
BcoDI	GTCTC	1 cut(s)	289
BfaI	CTAG	2 cut(s)	24, 495
BfrI	CTTAAG	2 cut(s)	104, 385
BisI	GCNGC	5 cut(s)	54, 765, 810, 1009, 1172
BlsI	GCNGC	5 cut(s)	55, 766, 811, 1010, 1173
BmeRI	GACNNNNNGTC	1 cut(s)	841
BmgBI	CACGTC	1 cut(s)	220
BmgT120I	GGNCC	2 cut(s)	566, 917
BmiI	GGNNCC	1 cut(s)	567
BmsI	GCATC	2 cut(s)	249, 422
BpiI	GAAGAC	1 cut(s)	772
BpmI	CTGGAG	1 cut(s)	879
Bpu10I	CCTNAGC	2 cut(s)	532, 871
Bpu14I	TTCGAA	1 cut(s)	487
BsaAI	YACGTR	1 cut(s)	92
BsaI	GGTCTC	1 cut(s)	289
BsaJI	CCNNGG	2 cut(s)	985, 1036
BsaWI	WCCGGW	1 cut(s)	64
Bsc4I	CCNNNNNNNGG	1 cut(s)	159
Bse1I	ACTGG	2 cut(s)	638, 920
BseDI	CCNNGG	2 cut(s)	985, 1036
BseGI	GGATG	4 cut(s)	213, 621, 753, 1032
BseLI	CCNNNNNNNGG	1 cut(s)	159
BseMII	CTCAG	2 cut(s)	455, 885
BseNI	ACTGG	2 cut(s)	638, 920
BseRI	GAGGAG	2 cut(s)	83, 128
BseSI	GKGCMC	1 cut(s)	905
BseX3I	CGGCCG	1 cut(s)	54
BseXI	GCAGC	4 cut(s)	776, 796, 1020, 1183
BsgI	GTGCAG	2 cut(s)	576, 708
Bsh1285I	CGRYCG	1 cut(s)	57
BshFI	GGCC	4 cut(s)	56, 568, 589, 919
BsiEI	CGRYCG	1 cut(s)	57
BsiHKAI	GWGCWC	3 cut(s)	11, 872, 905
BsiSI	CCGG	3 cut(s)	57, 65, 249
BslFI	GGGAC	2 cut(s)	123, 421
BslI	CCNNNNNNNGG	1 cut(s)	159
BsmAI	GTCTC	1 cut(s)	289
BsmFI	GGGAC	2 cut(s)	123, 421
BsnI	GGCC	4 cut(s)	56, 568, 589, 919
Bso31I	GGTCTC	1 cut(s)	289
Bsp119I	TTCGAA	1 cut(s)	487
Bsp1286I	GDGCHC	3 cut(s)	11, 872, 905
Bsp1407I	TGTACA	1 cut(s)	558
Bsp143I	GATC	1 cut(s)	1146
BspACI	CCGC	3 cut(s)	53, 536, 1091
BspANI	GGCC	4 cut(s)	56, 568, 589, 919
BspCNI	CTCAG	2 cut(s)	456, 884
BspHI	TCATGA	1 cut(s)	141
BspLI	GGNNCC	1 cut(s)	567
BspQI	GCTCTTC	1 cut(s)	1116
BspT104I	TTCGAA	1 cut(s)	487
BspTI	CTTAAG	2 cut(s)	104, 385
BspTNI	GGTCTC	1 cut(s)	289
BsrGI	TGTACA	1 cut(s)	558
BsrI	ACTGG	2 cut(s)	638, 920
BssECI	CCNNGG	2 cut(s)	985, 1036
BssMI	GATC	1 cut(s)	1146
BssNAI	GTATAC	1 cut(s)	165
BssSI	CACGAG	1 cut(s)	83
BssT1I	CCWWGG	1 cut(s)	1036
Bst1107I	GTATAC	1 cut(s)	165
Bst2BI	CACGAG	1 cut(s)	83
Bst4CI	ACNGT	2 cut(s)	658, 787
Bst6I	CTCTTC	3 cut(s)	903, 1089, 1116
BstAFI	CTTAAG	2 cut(s)	104, 385
BstAUI	TGTACA	1 cut(s)	558
BstBAI	YACGTR	1 cut(s)	92
BstBI	TTCGAA	1 cut(s)	487
BstC8I	GCNNGC	4 cut(s)	382, 591, 699, 1006
BstDEI	CTNAG	3 cut(s)	464, 532, 871
BstF5I	GGATG	4 cut(s)	213, 621, 753, 1032
BstKTI	GATC	1 cut(s)	1149
BstMAI	GTCTC	1 cut(s)	289
BstMBI	GATC	1 cut(s)	1146
BstMCI	CGRYCG	1 cut(s)	57
BstMWI	GCNNNNNNNGC	3 cut(s)	15, 773, 971
BstNSI	RCATGY	1 cut(s)	373
BstSLI	GKGCMC	1 cut(s)	905
BstSNI	TACGTA	1 cut(s)	92
BstV1I	GCAGC	4 cut(s)	776, 796, 1020, 1183
BstV2I	GAAGAC	1 cut(s)	772
BstZ17I	GTATAC	1 cut(s)	165
BstZI	CGGCCG	1 cut(s)	54
BsuRI	GGCC	4 cut(s)	56, 568, 589, 919
BtrI	CACGTC	1 cut(s)	220
BtsCI	GGATG	4 cut(s)	213, 621, 753, 1032
Cac8I	GCNNGC	4 cut(s)	382, 591, 699, 1006
CciI	TCATGA	1 cut(s)	141
Cfr13I	GGNCC	2 cut(s)	566, 917
CseI	GACGC	2 cut(s)	180, 423
Csp6I	GTAC	5 cut(s)	373, 461, 559, 688, 1142
CspCI	CAANNNNNGTGG	2 cut(s)	942, 977
CviQI	GTAC	5 cut(s)	373, 461, 559, 688, 1142
DdeI	CTNAG	3 cut(s)	464, 532, 871
DpnI	GATC	1 cut(s)	1148
DpnII	GATC	1 cut(s)	1146
DraIII	CACNNNGTG	1 cut(s)	789
DriI	GACNNNNNGTC	1 cut(s)	841
EaeI	YGGCCR	1 cut(s)	54
EagI	CGGCCG	1 cut(s)	54
Eam1104I	CTCTTC	3 cut(s)	903, 1089, 1116
Eam1105I	GACNNNNNGTC	1 cut(s)	841
EarI	CTCTTC	3 cut(s)	903, 1089, 1116
EciI	GGCGGA	1 cut(s)	1106
EclXI	CGGCCG	1 cut(s)	54
Eco105I	TACGTA	1 cut(s)	92
Eco130I	CCWWGG	1 cut(s)	1036
Eco31I	GGTCTC	1 cut(s)	289
Eco32I	GATATC	1 cut(s)	719
Eco52I	CGGCCG	1 cut(s)	54
Eco57I	CTGAAG	1 cut(s)	1137
EcoO109I	RGGNCCY	1 cut(s)	566
EcoRV	GATATC	1 cut(s)	719
EcoT14I	CCWWGG	1 cut(s)	1036
ErhI	CCWWGG	1 cut(s)	1036
FaqI	GGGAC	2 cut(s)	123, 421
FauI	CCCGC	1 cut(s)	529
FblI	GTMKAC	2 cut(s)	164, 222
Fnu4HI	GCNGC	5 cut(s)	54, 765, 810, 1009, 1172
FokI	GGATG	4 cut(s)	220, 608, 760, 1019
Fsp4HI	GCNGC	5 cut(s)	54, 765, 810, 1009, 1172
FspBI	CTAG	2 cut(s)	24, 495
GluI	GCNGC	5 cut(s)	54, 765, 810, 1009, 1172
GsuI	CTGGAG	1 cut(s)	879
HaeIII	GGCC	4 cut(s)	56, 568, 589, 919
HapII	CCGG	3 cut(s)	57, 65, 249
HgaI	GACGC	2 cut(s)	180, 423
HincII	GTYRAC	2 cut(s)	424, 835
HindII	GTYRAC	2 cut(s)	424, 835
HindIII	AAGCTT	1 cut(s)	382
HinfI	GANTC	2 cut(s)	825, 1097
HpaII	CCGG	3 cut(s)	57, 65, 249
HphI	GGTGA	3 cut(s)	775, 1001, 1082
Hpy166II	GTNNAC	6 cut(s)	165, 223, 424, 835, 903, 1181
Hpy188I	TCNGA	3 cut(s)	402, 824, 1110
Hpy188III	TCNNGA	4 cut(s)	24, 142, 281, 479
Hpy8I	GTNNAC	6 cut(s)	165, 223, 424, 835, 903, 1181
HpyAV	CCTTC	6 cut(s)	35, 205, 415, 494, 602, 1061
HpyCH4III	ACNGT	2 cut(s)	658, 787
HpyCH4IV	ACGT	4 cut(s)	91, 219, 831, 1083
HpyF10VI	GCNNNNNNNGC	3 cut(s)	15, 773, 971
HpyF3I	CTNAG	3 cut(s)	464, 532, 871
HpySE526I	ACGT	4 cut(s)	91, 219, 831, 1083
Kzo9I	GATC	1 cut(s)	1146
LguI	GCTCTTC	1 cut(s)	1116
LmnI	GCTCC	2 cut(s)	14, 267
Lsp1109I	GCAGC	4 cut(s)	776, 796, 1020, 1183
LweI	GCATC	2 cut(s)	249, 422
MaeI	CTAG	2 cut(s)	24, 495
MaeII	ACGT	4 cut(s)	91, 219, 831, 1083
MaeIII	GTNAC	3 cut(s)	87, 98, 928
MalI	GATC	1 cut(s)	1148
MboI	GATC	1 cut(s)	1146
MboII	GAAGA	8 cut(s)	50, 57, 726, 772, 877, 920, 1106, 1133
MfeI	CAATTG	1 cut(s)	456
MhlI	GDGCHC	3 cut(s)	11, 872, 905
MluCI	AATT	6 cut(s)	450, 456, 580, 648, 954, 1077
MlyI	GAGTC	1 cut(s)	1106
MmeI	TCCRAC	2 cut(s)	425, 450
MseI	TTAA	5 cut(s)	105, 365, 386, 638, 939
MspA1I	CMGCKG	1 cut(s)	776
MspCI	CTTAAG	2 cut(s)	104, 385
MspI	CCGG	3 cut(s)	57, 65, 249
MunI	CAATTG	1 cut(s)	456
MwoI	GCNNNNNNNGC	3 cut(s)	15, 773, 971
NdeII	GATC	1 cut(s)	1146
NlaIV	GGNNCC	1 cut(s)	567
NmuCI	GTSAC	1 cut(s)	928
NspI	RCATGY	1 cut(s)	373
NspV	TTCGAA	1 cut(s)	487
PagI	TCATGA	1 cut(s)	141
PciI	ACATGT	1 cut(s)	369
PciSI	GCTCTTC	1 cut(s)	1116
PcsI	WCGNNNNNNNCGW	1 cut(s)	828
PfeI	GAWTC	1 cut(s)	825
PkrI	GCNGC	5 cut(s)	55, 766, 811, 1010, 1173
PleI	GAGTC	1 cut(s)	1105
PpsI	GAGTC	1 cut(s)	1105
Ppu21I	YACGTR	1 cut(s)	92
PscI	ACATGT	1 cut(s)	369
Psp1406I	AACGTT	1 cut(s)	831
PspN4I	GGNNCC	1 cut(s)	567
PspPI	GGNCC	2 cut(s)	566, 917
PsrI	GAACNNNNNNTAC	2 cut(s)	318, 350
PvuII	CAGCTG	1 cut(s)	776
RsaI	GTAC	5 cut(s)	374, 462, 560, 689, 1143
RsaNI	GTAC	5 cut(s)	373, 461, 559, 688, 1142
SapI	GCTCTTC	1 cut(s)	1116
SaqAI	TTAA	5 cut(s)	105, 365, 386, 638, 939
SatI	GCNGC	5 cut(s)	54, 765, 810, 1009, 1172
Sau3AI	GATC	1 cut(s)	1146
Sau96I	GGNCC	2 cut(s)	566, 917
SchI	GAGTC	1 cut(s)	1106
SduI	GDGCHC	3 cut(s)	11, 872, 905
SfaNI	GCATC	2 cut(s)	249, 422
SfuI	TTCGAA	1 cut(s)	487
SmlI	CTYRAG	2 cut(s)	104, 385
SmoI	CTYRAG	2 cut(s)	104, 385
SnaBI	TACGTA	1 cut(s)	92
Sse9I	AATT	6 cut(s)	450, 456, 580, 648, 954, 1077
SsiI	CCGC	3 cut(s)	53, 536, 1091
SspMI	CTAG	2 cut(s)	24, 495
StyI	CCWWGG	1 cut(s)	1036
TaaI	ACNGT	2 cut(s)	658, 787
TaiI	ACGT	4 cut(s)	94, 222, 834, 1086
TaqI	TCGA	3 cut(s)	235, 487, 845
TaqII	GACCGA	1 cut(s)	313
TasI	AATT	6 cut(s)	450, 456, 580, 648, 954, 1077
TatI	WGTACW	3 cut(s)	372, 460, 558
TauI	GCSGC	1 cut(s)	56
TfiI	GAWTC	1 cut(s)	825
Tru1I	TTAA	5 cut(s)	105, 365, 386, 638, 939
Tru9I	TTAA	5 cut(s)	105, 365, 386, 638, 939
TseFI	GTSAC	1 cut(s)	928
TseI	GCWGC	4 cut(s)	764, 809, 1008, 1171
Tsp45I	GTSAC	1 cut(s)	928
TspDTI	ATGAA	5 cut(s)	224, 877, 1015, 1089, 1134
Vha464I	CTTAAG	2 cut(s)	104, 385
VneI	GTGCAC	1 cut(s)	901
XapI	RAATTY	2 cut(s)	580, 1077
XbaI	TCTAGA	1 cut(s)	23
XceI	RCATGY	1 cut(s)	373
XmiI	GTMKAC	2 cut(s)	164, 222
XspI	CTAG	2 cut(s)	24, 495

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.