Rh6CG321100

WAT1-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0002399
Physical Location & Seq
Reverse (-)
50650478 .. 50679711
29234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG321100.1

Sequence Viewer

Length: 870 bp
ATGAAAAGCAGGGTTAGCTTAACCCTTAATATGTATTTTGCAAGCCTGAAGTACACATCTTCAAGCTTCGTCACATCAATAGCCAATACCATACCATCCCTGACTTTTCTGATTGCAGTCATACTCAGGATGGAGGCTCTAGATGTAAGGAATCCGCGCGGAATAGCTAAACTCTTGGGAACCTTAATATCTCTGGCTGGGGTCATCACCATGACTTCGTACAAAGGACCTGCAATACAAAGCTTCTCGGGAGCATCACAACACATAAGAAGTAATTTTGTTCACAAAAGCTGGACAAAGGGTTCAATCCTTGTGATTGCAAGTTGTATAACATGGTCCATATGGTACATAATGCAGGGGATCACATTGAAGAAATATCCTGCACAACTGTCACTAACCACATGGATCAATTGTATCGGTGCAGCACAATCTGCTGTGTTCACAGTTATCATACAGCACAAACGGGCAGCATGGAGAATTACATACAACAATGATTTCTGGTCCATCATATATGCTGGAGTTGTATGCTCAGGTATAACAATCTTCATTCAACTATGGTGTACAAAACAAAAAGGACCTGTCTTCGTGACCATGTTCAGTCCTCTTGCAACAGCATTAGTGGCTATTCTAGCTTACTTCATTCTCGGCGAAAAGCTGCACATTGGCAGAATAGTGGGAGCGGTCATTATCATTATCGGTCTATACTTAGTCTTATGGGGCAAAGATAGAGATCAAAATCATTTCAAATCCCAAGAGCAACCTACCGTGATTAGTGATAACCAGAAGGATCCCAAGATGCAAGTGGGATCTTCAGCAGAAAGACAGGTCCTCCAAATGGACCTTGAGGAAAAAGATAAAACCGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

289

Amino Acids

32.13

Weight (kDa)

9.67

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 100 - 238 5.5e-17 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 238
AccBSI CCGCTC 1 cut(s) 680
AccII CGCG 2 cut(s) 157, 159
AciI CCGC 3 cut(s) 155, 159, 680
AclWI GGATC 5 cut(s) 368, 413, 782, 795, 814
AcuI CTGAAG 2 cut(s) 68, 795
AfaI GTAC 4 cut(s) 53, 221, 347, 562
AfiI CCNNNNNNNGG 1 cut(s) 835
AgsI TTSAA 5 cut(s) 63, 306, 370, 551, 745
AloI GAACNNNNNNTCC 2 cut(s) 286, 318
AluBI AGCT 7 cut(s) 18, 66, 167, 243, 291, 632, 655
AluI AGCT 7 cut(s) 18, 66, 167, 243, 291, 632, 655
AlwI GGATC 5 cut(s) 368, 413, 782, 795, 814
Ama87I CYCGRG 1 cut(s) 247
ApeKI GCWGC 3 cut(s) 422, 467, 655
AspLEI GCGC 1 cut(s) 159
AspS9I GGNCC 6 cut(s) 227, 336, 501, 575, 826, 838
AsuHPI GGTGA 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 247
AvaII GGWCC 6 cut(s) 227, 336, 501, 575, 826, 838
BamHI GGATCC 1 cut(s) 787
BbsI GAAGAC 1 cut(s) 574
BbvI GCAGC 3 cut(s) 434, 479, 642
BccI CCATC 4 cut(s) 103, 124, 512, 857
BfaI CTAG 2 cut(s) 140, 629
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 3 cut(s) 423, 468, 656
BlsI GCNGC 3 cut(s) 424, 469, 657
Bme18I GGWCC 6 cut(s) 227, 336, 501, 575, 826, 838
BmeT110I CYCGRG 1 cut(s) 247
BmgT120I GGNCC 6 cut(s) 227, 336, 501, 575, 826, 838
BmiI GGNNCC 2 cut(s) 181, 789
BmsI GCATC 2 cut(s) 263, 786
BpiI GAAGAC 1 cut(s) 574
BpmI CTGGAG 1 cut(s) 537
Bpu10I CCTNAGC 1 cut(s) 529
BpuEI CTTGAG 1 cut(s) 863
BsaBI GATNNNNATC 2 cut(s) 729, 735
BsaXI ACNNNNNCTCC 2 cut(s) 813, 843
Bsc4I CCNNNNNNNGG 1 cut(s) 835
Bse8I GATNNNNATC 2 cut(s) 729, 735
BseGI GGATG 2 cut(s) 95, 135
BseJI GATNNNNATC 2 cut(s) 729, 735
BseLI CCNNNNNNNGG 1 cut(s) 835
BseMII CTCAG 2 cut(s) 139, 543
BseXI GCAGC 3 cut(s) 434, 479, 642
BseYI CCCAGC 1 cut(s) 197
BsgI GTGCAG 3 cut(s) 366, 441, 641
Bsh1236I CGCG 2 cut(s) 157, 159
BsiHKCI CYCGRG 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 835
BsoBI CYCGRG 1 cut(s) 247
Bsp1407I TGTACA 1 cut(s) 560
Bsp143I GATC 5 cut(s) 360, 405, 730, 787, 806
BspACI CCGC 3 cut(s) 155, 159, 680
BspCNI CTCAG 2 cut(s) 138, 542
BspFNI CGCG 2 cut(s) 157, 159
BspLI GGNNCC 2 cut(s) 181, 789
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 5 cut(s) 368, 413, 782, 795, 814
BsrBI CCGCTC 1 cut(s) 680
BsrGI TGTACA 1 cut(s) 560
BssMI GATC 5 cut(s) 360, 405, 730, 787, 806
Bst4CI ACNGT 3 cut(s) 390, 445, 766
BstAPI GCANNNNNTGC 1 cut(s) 431
BstAUI TGTACA 1 cut(s) 560
BstC8I GCNNGC 1 cut(s) 43
BstDEI CTNAG 3 cut(s) 125, 529, 706
BstF5I GGATG 2 cut(s) 95, 135
BstFNI CGCG 2 cut(s) 157, 159
BstHHI GCGC 1 cut(s) 159
BstKTI GATC 5 cut(s) 363, 408, 733, 790, 809
BstMBI GATC 5 cut(s) 360, 405, 730, 787, 806
BstMWI GCNNNNNNNGC 4 cut(s) 15, 431, 620, 629
BstUI CGCG 2 cut(s) 157, 159
BstV1I GCAGC 3 cut(s) 434, 479, 642
BstV2I GAAGAC 1 cut(s) 574
BstX2I RGATCY 2 cut(s) 787, 806
BstYI RGATCY 2 cut(s) 787, 806
BtsCI GGATG 2 cut(s) 95, 135
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 43
CfoI GCGC 1 cut(s) 159
Cfr13I GGNCC 6 cut(s) 227, 336, 501, 575, 826, 838
Csp6I GTAC 4 cut(s) 52, 220, 346, 561
CviAII CATG 5 cut(s) 211, 333, 402, 471, 592
CviQI GTAC 4 cut(s) 52, 220, 346, 561
DdeI CTNAG 3 cut(s) 125, 529, 706
DpnI GATC 5 cut(s) 362, 407, 732, 789, 808
DpnII GATC 5 cut(s) 360, 405, 730, 787, 806
Eco47I GGWCC 6 cut(s) 227, 336, 501, 575, 826, 838
Eco57I CTGAAG 2 cut(s) 68, 795
Eco88I CYCGRG 1 cut(s) 247
EcoO109I RGGNCCY 3 cut(s) 227, 575, 826
FaeI CATG 5 cut(s) 214, 336, 405, 474, 595
FatI CATG 5 cut(s) 210, 332, 401, 470, 591
FauNDI CATATG 1 cut(s) 341
Fnu4HI GCNGC 3 cut(s) 423, 468, 656
FokI GGATG 2 cut(s) 82, 142
Fsp4HI GCNGC 3 cut(s) 423, 468, 656
FspBI CTAG 2 cut(s) 140, 629
GlaI GCGC 1 cut(s) 158
GluI GCNGC 3 cut(s) 423, 468, 656
GsaI CCCAGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 537
HhaI GCGC 1 cut(s) 159
Hin1II CATG 5 cut(s) 214, 336, 405, 474, 595
Hin6I GCGC 1 cut(s) 157
HinP1I GCGC 1 cut(s) 157
HindIII AAGCTT 2 cut(s) 64, 241
HinfI GANTC 1 cut(s) 151
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 4 cut(s) 54, 283, 441, 561
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 4 cut(s) 127, 140, 249, 586
Hpy8I GTNNAC 4 cut(s) 54, 283, 441, 561
HpyAV CCTTC 1 cut(s) 778
HpyCH4III ACNGT 3 cut(s) 390, 445, 766
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 431, 620, 629
HpyF3I CTNAG 3 cut(s) 125, 529, 706
Hsp92II CATG 5 cut(s) 214, 336, 405, 474, 595
HspAI GCGC 1 cut(s) 157
Kzo9I GATC 5 cut(s) 360, 405, 730, 787, 806
LmnI GCTCC 2 cut(s) 251, 677
Lsp1109I GCAGC 3 cut(s) 434, 479, 642
LweI GCATC 2 cut(s) 263, 786
MaeI CTAG 2 cut(s) 140, 629
MaeIII GTNAC 3 cut(s) 70, 390, 586
MalI GATC 5 cut(s) 362, 407, 732, 789, 808
MbiI CCGCTC 1 cut(s) 680
MboI GATC 5 cut(s) 360, 405, 730, 787, 806
MboII GAAGA 5 cut(s) 51, 382, 535, 574, 801
MfeI CAATTG 1 cut(s) 409
MflI RGATCY 2 cut(s) 787, 806
MluCI AATT 3 cut(s) 274, 409, 477
MnlI CCTC 4 cut(s) 127, 612, 838, 839
MseI TTAA 3 cut(s) 20, 27, 185
MslI CAYNNNNRTG 1 cut(s) 209
MunI CAATTG 1 cut(s) 409
MvnI CGCG 2 cut(s) 157, 159
MwoI GCNNNNNNNGC 4 cut(s) 15, 431, 620, 629
NdeI CATATG 1 cut(s) 341
NdeII GATC 5 cut(s) 360, 405, 730, 787, 806
NlaIII CATG 5 cut(s) 214, 336, 405, 474, 595
NlaIV GGNNCC 2 cut(s) 181, 789
NmeAIII GCCGAG 1 cut(s) 624
NmuCI GTSAC 3 cut(s) 70, 390, 586
PfeI GAWTC 1 cut(s) 151
PkrI GCNGC 3 cut(s) 424, 469, 657
PpuMI RGGWCCY 3 cut(s) 227, 575, 826
Psp5II RGGWCCY 3 cut(s) 227, 575, 826
PspFI CCCAGC 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 181, 789
PspPI GGNCC 6 cut(s) 227, 336, 501, 575, 826, 838
PspPPI RGGWCCY 3 cut(s) 227, 575, 826
PsuI RGATCY 2 cut(s) 787, 806
RsaI GTAC 4 cut(s) 53, 221, 347, 562
RsaNI GTAC 4 cut(s) 52, 220, 346, 561
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 3 cut(s) 20, 27, 185
SatI GCNGC 3 cut(s) 423, 468, 656
Sau3AI GATC 5 cut(s) 360, 405, 730, 787, 806
Sau96I GGNCC 6 cut(s) 227, 336, 501, 575, 826, 838
SfaNI GCATC 2 cut(s) 263, 786
SinI GGWCC 6 cut(s) 227, 336, 501, 575, 826, 838
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 842
SmoI CTYRAG 1 cut(s) 842
Sse9I AATT 3 cut(s) 274, 409, 477
SsiI CCGC 3 cut(s) 155, 159, 680
SspMI CTAG 2 cut(s) 140, 629
TaaI ACNGT 3 cut(s) 390, 445, 766
TaqII GACCGA 1 cut(s) 686
TasI AATT 3 cut(s) 274, 409, 477
TatI WGTACW 2 cut(s) 51, 560
TfiI GAWTC 1 cut(s) 151
Tru1I TTAA 3 cut(s) 20, 27, 185
Tru9I TTAA 3 cut(s) 20, 27, 185
TseFI GTSAC 3 cut(s) 70, 390, 586
TseI GCWGC 3 cut(s) 422, 467, 655
Tsp45I GTSAC 3 cut(s) 70, 390, 586
TspDTI ATGAA 3 cut(s) 17, 535, 628
VpaK11BI GGWCC 6 cut(s) 227, 336, 501, 575, 826, 838
XbaI TCTAGA 1 cut(s) 139
XcmI CCANNNNNNNNNTGG 1 cut(s) 799
XspI CTAG 2 cut(s) 140, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.