FvH4_2g27460

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003233
Physical Location & Seq
Reverse (-)
21794614 .. 21797358
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27460.t1

Sequence Viewer

Length: 615 bp
ATGTCGATCTTCGAGTATAATGGAGGCGCTATAATCGCAATGGTGGGGAAGAACTGCTTCGCCATCGCCTCCGATCGCCGCCTCGGAGTTCAGCTCCAGACAATCGCCACCGACTTCAAGAAGATTTACAGAGTCAGCGACCGGCTGTTTCTCGGCCTCTCCGGCCTCGCCACCGATGCTCAGACCCTGTTTCAGAGGCTTATGTTTAAGCACAAGCTGTATCAGCTGAGGGAAGAGAGGGATATGAAGCCTGAGACTTTCGCTAGCCTCGTCTCCGCTATTTTGTACGAGAAAAGGTTTGGACCATACTTCATTCAGCCTGTGATTGCTGGATTAGGAGATGGAGACAAGCCATTCATCTGTACTATGGATTCAATTGGAGCCAAGGAACTGGCAAAGGATTTTGTTGTTGCCGGCACTGCATCAGAGTCCCTATATGGTGCTTGTGAGGCGATGTTCAAGCCTGACATGGAACCAGAGGAATTATTTGAGACTGTTTCTCAAGCACTTCTATCATCAGTGGATCGAGACTGTTTGAGTGGATGGGGTGGCCATATCTACGTTGTGACACCAACCGAGGTGAAGGAGAGGATCTTGAAGGGACGAATGGATTGA

Protein Analysis

205

Amino Acids

22.73

Weight (kDa)

5.54

Isoelectric Point (pI)

31.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 7 - 189 1.5e-46 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 79, 276
AclWI GGATC 2 cut(s) 531, 599
AcoI YGGCCR 1 cut(s) 550
AfaI GTAC 2 cut(s) 287, 364
AgsI TTSAA 4 cut(s) 118, 375, 460, 598
AluBI AGCT 3 cut(s) 94, 217, 226
AluI AGCT 3 cut(s) 94, 217, 226
Alw26I GTCTC 5 cut(s) 248, 277, 339, 485, 522
AlwI GGATC 2 cut(s) 531, 599
AlwNI CAGNNNCTG 1 cut(s) 187
AoxI GGCC 3 cut(s) 154, 163, 550
Asp700I GAANNNNTTC 1 cut(s) 56
AspLEI GCGC 1 cut(s) 29
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 592
AsuNHI GCTAGC 1 cut(s) 263
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 552
BbvCI CCTCAGC 1 cut(s) 227
BccI CCATC 3 cut(s) 71, 335, 537
BcoDI GTCTC 5 cut(s) 248, 277, 339, 485, 522
BfaI CTAG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 30
BglI GCCNNNNNGGC 1 cut(s) 162
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 382, 474
BmsI GCATC 2 cut(s) 166, 431
BmtI GCTAGC 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 78, 110
BpmI CTGGAG 1 cut(s) 80
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 82, 384, 576
Bse118I RCCGGY 2 cut(s) 141, 413
Bse1I ACTGG 1 cut(s) 396
Bse3DI GCAATG 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 82, 384, 576
BseGI GGATG 1 cut(s) 548
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 3 cut(s) 194, 218, 243
BseNI ACTGG 1 cut(s) 396
Bsh1285I CGRYCG 2 cut(s) 76, 142
BshFI GGCC 3 cut(s) 156, 165, 552
BsiEI CGRYCG 2 cut(s) 76, 142
BsiSI CCGG 3 cut(s) 142, 162, 414
BslFI GGGAC 1 cut(s) 415
BsmAI GTCTC 5 cut(s) 248, 277, 339, 485, 522
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 3 cut(s) 156, 165, 552
Bsp143I GATC 4 cut(s) 6, 73, 523, 591
BspACI CCGC 2 cut(s) 79, 276
BspANI GGCC 3 cut(s) 156, 165, 552
BspCNI CTCAG 3 cut(s) 193, 219, 244
BspLI GGNNCC 2 cut(s) 382, 474
BspOI GCTAGC 1 cut(s) 267
BspPI GGATC 2 cut(s) 531, 599
BsrDI GCAATG 1 cut(s) 45
BsrFI RCCGGY 2 cut(s) 141, 413
BsrI ACTGG 1 cut(s) 396
BssAI RCCGGY 2 cut(s) 141, 413
BssECI CCNNGG 3 cut(s) 82, 384, 576
BssMI GATC 4 cut(s) 6, 73, 523, 591
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 2 cut(s) 496, 533
Bst6I CTCTTC 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 265, 415
BstDEI CTNAG 3 cut(s) 180, 227, 252
BstF5I GGATG 1 cut(s) 548
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 1 cut(s) 29
BstKTI GATC 4 cut(s) 9, 76, 526, 594
BstMAI GTCTC 5 cut(s) 248, 277, 339, 485, 522
BstMBI GATC 4 cut(s) 6, 73, 523, 591
BstMCI CGRYCG 2 cut(s) 76, 142
BstMWI GCNNNNNNNGC 6 cut(s) 35, 162, 176, 223, 419, 449
BstX2I RGATCY 1 cut(s) 591
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 1 cut(s) 591
BsuRI GGCC 3 cut(s) 156, 165, 552
BtgZI GCGATG 2 cut(s) 49, 467
BtsCI GGATG 1 cut(s) 548
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 417, 525
Cac8I GCNNGC 2 cut(s) 265, 415
CaiI CAGNNNCTG 1 cut(s) 187
CfoI GCGC 1 cut(s) 29
Cfr10I RCCGGY 2 cut(s) 141, 413
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 286, 363
CviAII CATG 1 cut(s) 469
CviQI GTAC 2 cut(s) 286, 363
DdeI CTNAG 3 cut(s) 180, 227, 252
DpnI GATC 4 cut(s) 8, 75, 525, 593
DpnII GATC 4 cut(s) 6, 73, 523, 591
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 1 cut(s) 472
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 1 cut(s) 415
FatI CATG 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 1 cut(s) 555
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 1 cut(s) 264
GlaI GCGC 1 cut(s) 28
GluI GCNGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 80
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 3 cut(s) 156, 165, 552
HapII CCGG 3 cut(s) 142, 162, 414
HhaI GCGC 1 cut(s) 29
Hin1II CATG 1 cut(s) 472
Hin6I GCGC 1 cut(s) 27
HinP1I GCGC 1 cut(s) 27
HinfI GANTC 3 cut(s) 132, 371, 428
HpaII CCGG 3 cut(s) 142, 162, 414
HphI GGTGA 1 cut(s) 592
Hpy188I TCNGA 5 cut(s) 73, 86, 183, 195, 427
Hpy188III TCNNGA 4 cut(s) 97, 118, 527, 595
HpyAV CCTTC 2 cut(s) 577, 592
HpyCH4III ACNGT 2 cut(s) 496, 533
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 162, 176, 223, 419, 449
HpyF3I CTNAG 3 cut(s) 180, 227, 252
HpySE526I ACGT 1 cut(s) 561
Hsp92II CATG 1 cut(s) 472
HspAI GCGC 1 cut(s) 27
KroI GCCGGC 1 cut(s) 413
KroNI GCCGGC 1 cut(s) 415
Kzo9I GATC 4 cut(s) 6, 73, 523, 591
LmnI GCTCC 2 cut(s) 99, 380
LweI GCATC 2 cut(s) 166, 431
MaeI CTAG 1 cut(s) 264
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 1 cut(s) 565
MalI GATC 4 cut(s) 8, 75, 525, 593
MboI GATC 4 cut(s) 6, 73, 523, 591
MboII GAAGA 3 cut(s) 61, 133, 245
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 1 cut(s) 591
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 2 cut(s) 375, 482
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 2 cut(s) 141, 437
Mox20I TGGCCA 1 cut(s) 552
MroNI GCCGGC 1 cut(s) 413
MroXI GAANNNNTTC 1 cut(s) 56
MscI TGGCCA 1 cut(s) 552
MseI TTAA 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 3 cut(s) 142, 162, 414
MunI CAATTG 1 cut(s) 375
MwoI GCNNNNNNNGC 6 cut(s) 35, 162, 176, 223, 419, 449
NaeI GCCGGC 1 cut(s) 415
NdeII GATC 4 cut(s) 6, 73, 523, 591
NgoMIV GCCGGC 1 cut(s) 413
NheI GCTAGC 1 cut(s) 263
NlaIII CATG 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 382, 474
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PdiI GCCGGC 1 cut(s) 415
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 1 cut(s) 80
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 2 cut(s) 140, 436
PpsI GAGTC 2 cut(s) 140, 436
PspN4I GGNNCC 2 cut(s) 382, 474
PspPI GGNCC 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 591
PvuI CGATCG 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 226
RsaI GTAC 2 cut(s) 287, 364
RsaNI GTAC 2 cut(s) 286, 363
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 4 cut(s) 6, 73, 523, 591
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 2 cut(s) 141, 437
SetI ASST 6 cut(s) 96, 219, 228, 299, 564, 582
SfaNI GCATC 2 cut(s) 166, 431
SfiI GGCCNNNNNGGCC 1 cut(s) 162
SinI GGWCC 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 2 cut(s) 375, 482
SsiI CCGC 2 cut(s) 79, 276
SspMI CTAG 1 cut(s) 264
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 2 cut(s) 496, 533
TaiI ACGT 1 cut(s) 564
TaqI TCGA 3 cut(s) 5, 12, 526
TasI AATT 2 cut(s) 375, 482
TatI WGTACW 1 cut(s) 362
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 2 cut(s) 424, 525
TseFI GTSAC 1 cut(s) 565
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 3 cut(s) 260, 301, 346
TspRI CASTG 2 cut(s) 424, 525
VpaK11BI GGWCC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.