MD15G1344700.v1.1

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003233
Physical Location & Seq
Forward (+)
40366894 .. 40370138
3245 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1344700.v1.1.491

Sequence Viewer

Length: 615 bp
ATGTCGATCTTCGAGTACAATGGGAGTGCACTGGTCGCAATGGTGGGGAAGAACTGCTTCGCCATCGCCAGCGATCGCCGCCTCGGTGTTCAGCTCCAGACCGTCGCCACCGACTTCAAGAGGATTAGCCAAGTCCATGATAGGCTCTTCATCGGCCTCTCCGGCCTCGCCACGGACGCTCAGACTCTGTATCAGAGGCTCATGTTTCGGCACAAGCTGTACCAGCTTCGTGAGGAGAGGGATATGAAGCCCGAGACTTTTGCGAGCCTCGTTTCGGCTATTCTGTATGAGAAAAGGTTTGGTCCCTACTTCACTCAGCCTGTAATTGCTGGGTTAGGTGATGAAGACAAACCCTTCATCTGTACTATGGATTCAATTGGAGCCAAGGAACTAGCAAAAGACTTTGTTGTTGCTGGTACTGCATCCGAGTCCCTTTATGGTGCTTGTGAGGCAATGTTCAAGCCTGATATGGAACCAGAGGAATTGTTTGAGACTGTCTCTCAAGCATTGCTTTCATCAGTAGATCGAGACTGTCTGAGTGGTTGGGGAGGCCACGTCTTTGTCGTAACACCAACTGAAGTGCAGGAGAAGATCTTGAAGGGAAGGATGGACTGA

Protein Analysis

205

Amino Acids

22.76

Weight (kDa)

5.18

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 7 - 189 3.6e-45 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 4 cut(s) 17, 221, 364, 418
AfiI CCNNNNNNNGG 2 cut(s) 172, 274
AgsI TTSAA 4 cut(s) 118, 375, 460, 598
AjiI CACGTC 1 cut(s) 556
AluBI AGCT 3 cut(s) 94, 217, 226
AluI AGCT 3 cut(s) 94, 217, 226
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 4 cut(s) 248, 485, 502, 522
Alw44I GTGCAC 1 cut(s) 27
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 3 cut(s) 154, 163, 550
ApaLI GTGCAC 1 cut(s) 27
AsiSI GCGATCGC 1 cut(s) 76
Asp700I GAANNNNTTC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 350
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 302
BaeGI GKGCMC 1 cut(s) 31
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 31
BccI CCATC 2 cut(s) 71, 601
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BcoDI GTCTC 4 cut(s) 248, 485, 502, 522
BfaI CTAG 1 cut(s) 392
BglI GCCNNNNNGGC 1 cut(s) 162
BglII AGATCT 1 cut(s) 591
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 251
BmgBI CACGTC 1 cut(s) 556
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 3 cut(s) 304, 382, 474
BmsI GCATC 1 cut(s) 431
BpiI GAAGAC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 482, 514
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 82, 171, 384
BsaXI ACNNNNNCTCC 2 cut(s) 540, 570
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 274
Bse1I ACTGG 1 cut(s) 36
Bse3DI GCAATG 3 cut(s) 45, 459, 506
BseDI CCNNGG 3 cut(s) 82, 171, 384
BseGI GGATG 2 cut(s) 422, 612
BseLI CCNNNNNNNGG 2 cut(s) 172, 274
BseMI GCAATG 3 cut(s) 45, 459, 506
BseMII CTCAG 3 cut(s) 194, 329, 527
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 248
BseSI GKGCMC 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 329
BsgI GTGCAG 1 cut(s) 602
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 3 cut(s) 156, 165, 552
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 2 cut(s) 288, 415
BslI CCNNNNNNNGG 2 cut(s) 172, 274
BsmAI GTCTC 4 cut(s) 248, 485, 502, 522
BsmFI GGGAC 2 cut(s) 288, 415
BsnI GGCC 3 cut(s) 156, 165, 552
BsoBI CYCGRG 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 4 cut(s) 6, 73, 523, 591
BspACI CCGC 1 cut(s) 79
BspANI GGCC 3 cut(s) 156, 165, 552
BspCNI CTCAG 3 cut(s) 193, 328, 528
BspLI GGNNCC 3 cut(s) 304, 382, 474
BspQI GCTCTTC 1 cut(s) 152
BsrDI GCAATG 3 cut(s) 45, 459, 506
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 3 cut(s) 82, 171, 384
BssMI GATC 4 cut(s) 6, 73, 523, 591
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 3 cut(s) 103, 496, 533
Bst6I CTCTTC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 70, 265
BstDEI CTNAG 3 cut(s) 180, 315, 536
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 2 cut(s) 422, 612
BstKTI GATC 4 cut(s) 9, 76, 526, 594
BstMAI GTCTC 4 cut(s) 248, 485, 502, 522
BstMBI GATC 4 cut(s) 6, 73, 523, 591
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 7 cut(s) 35, 78, 162, 176, 223, 419, 449
BstSLI GKGCMC 1 cut(s) 31
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 591
BstYI RGATCY 1 cut(s) 591
BsuRI GGCC 3 cut(s) 156, 165, 552
BtgI CCRYGG 1 cut(s) 171
BtgZI GCGATG 1 cut(s) 49
BtrI CACGTC 1 cut(s) 556
BtsCI GGATG 2 cut(s) 422, 612
BtsIMutI CAGTG 1 cut(s) 29
Cac8I GCNNGC 2 cut(s) 70, 265
CaiI CAGNNNCTG 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 302
CseI GACGC 1 cut(s) 185
Csp6I GTAC 4 cut(s) 16, 220, 363, 417
CviAII CATG 2 cut(s) 137, 202
CviQI GTAC 4 cut(s) 16, 220, 363, 417
DdeI CTNAG 3 cut(s) 180, 315, 536
DpnI GATC 4 cut(s) 8, 75, 525, 593
DpnII GATC 4 cut(s) 6, 73, 523, 591
Eam1104I CTCTTC 1 cut(s) 152
EarI CTCTTC 1 cut(s) 152
Eco130I CCWWGG 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 2 cut(s) 140, 205
FaiI YATR 7 cut(s) 138, 203, 245, 288, 368, 438, 470
FalI AAGNNNNNCTT 4 cut(s) 41, 73, 495, 527
FaqI GGGAC 2 cut(s) 288, 415
FatI CATG 2 cut(s) 136, 201
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 1 cut(s) 409
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 1 cut(s) 392
GluI GCNGC 1 cut(s) 79
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 3 cut(s) 156, 165, 552
HapII CCGG 1 cut(s) 162
HgaI GACGC 1 cut(s) 185
Hin1II CATG 2 cut(s) 140, 205
HinfI GANTC 3 cut(s) 184, 371, 428
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 350
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 4 cut(s) 183, 195, 427, 537
Hpy188III TCNNGA 5 cut(s) 97, 118, 230, 527, 595
Hpy8I GTNNAC 1 cut(s) 29
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 364, 592, 597
HpyCH4III ACNGT 3 cut(s) 103, 496, 533
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 3 cut(s) 29, 422, 583
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 78, 162, 176, 223, 419, 449
HpyF3I CTNAG 3 cut(s) 180, 315, 536
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 2 cut(s) 140, 205
Kzo9I GATC 4 cut(s) 6, 73, 523, 591
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 2 cut(s) 99, 380
LweI GCATC 1 cut(s) 431
MaeI CTAG 1 cut(s) 392
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 565
MalI GATC 4 cut(s) 8, 75, 525, 593
MboI GATC 4 cut(s) 6, 73, 523, 591
MboII GAAGA 4 cut(s) 61, 139, 356, 601
MfeI CAATTG 1 cut(s) 375
MflI RGATCY 1 cut(s) 591
MhlI GDGCHC 1 cut(s) 31
MluCI AATT 3 cut(s) 324, 375, 482
MlyI GAGTC 2 cut(s) 178, 437
MroXI GAANNNNTTC 1 cut(s) 56
MspI CCGG 1 cut(s) 162
MunI CAATTG 1 cut(s) 375
MwoI GCNNNNNNNGC 7 cut(s) 35, 78, 162, 176, 223, 419, 449
NdeII GATC 4 cut(s) 6, 73, 523, 591
NlaIII CATG 2 cut(s) 140, 205
NlaIV GGNNCC 3 cut(s) 304, 382, 474
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 1 cut(s) 80
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 2 cut(s) 178, 436
PpsI GAGTC 2 cut(s) 178, 436
PspFI CCCAGC 1 cut(s) 329
PspN4I GGNNCC 3 cut(s) 304, 382, 474
PspPI GGNCC 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 591
PvuI CGATCG 1 cut(s) 76
RgaI GCGATCGC 1 cut(s) 76
RsaI GTAC 4 cut(s) 17, 221, 364, 418
RsaNI GTAC 4 cut(s) 16, 220, 363, 417
SapI GCTCTTC 1 cut(s) 152
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 4 cut(s) 6, 73, 523, 591
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 2 cut(s) 178, 437
SduI GDGCHC 1 cut(s) 31
SetI ASST 6 cut(s) 96, 219, 228, 299, 340, 558
SfaAI GCGATCGC 1 cut(s) 76
SfaNI GCATC 1 cut(s) 431
SfiI GGCCNNNNNGGCC 1 cut(s) 162
SgfI GCGATCGC 1 cut(s) 76
SinI GGWCC 1 cut(s) 302
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 3 cut(s) 324, 375, 482
SsiI CCGC 1 cut(s) 79
SspMI CTAG 1 cut(s) 392
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 3 cut(s) 103, 496, 533
TaiI ACGT 1 cut(s) 558
TaqI TCGA 3 cut(s) 5, 12, 526
TasI AATT 3 cut(s) 324, 375, 482
TatI WGTACW 2 cut(s) 15, 362
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 371
TscAI CASTG 1 cut(s) 36
TspDTI ATGAA 5 cut(s) 139, 260, 346, 357, 504
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 1 cut(s) 36
VneI GTGCAC 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.