RLG00000011708

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003233
Physical Location & Seq
Forward (+)
12065892 .. 12068824
2933 bp
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UTR
Exon/CDS
Intron
RLM00000011708

Sequence Viewer

Length: 615 bp
ATGTCGATCTTCGAGTATAATGGAGGTGCTATAATCGCAATGGTGGGGAAGAACTGCTTCGCCATCGCCTCCGATCGCCGCCTCGGCGTTCAGCTCCAGACAATCGCCACCGACTTCAAGAAGATTTACAGAGTCAGCGATAGGCTTTTCCTCGGTCTCTCCGGCCTCGCCACCGATGCTCAGACTCTGTTTCAGAGGCTTATGTTTAAGCATAAGCTGTACCAGCTGAGGGAAGAGAGGGATATGAAGCCTGAGACTTTTGCTAGTCTCGTCTCGGCTATTCTCTACGAGAAAAGGTTTGGTCCATACTTCATTCAGCCTGTGATTGCTGGATTGGGAGATGAAGACAAGCCGTTCATCTGTACTATGGATTCAATCGGAGCCAAGGAACTGGCGAAGGATTTTGTTGTTGCTGGCACTGCATCAGAGTCCCTTTATGGTGCTTGTGAGGCGATGTTCAAGCCTGACATGGAACCTGAGGAATTATTTGAGACTGTATCTCAAGCACTTCTATCATCAGTGGATCGAGACTGTTTGAGTGGATGGGGTGGCCATATCTATGTTGTGACACCAACCGAGGTGAAGGAGAGGATCTTGAAGGGACGGATGGATTGA

Protein Analysis

205

Amino Acids

22.81

Weight (kDa)

5.36

Isoelectric Point (pI)

32.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 7 - 189 3.9e-47 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 79
AclWI GGATC 2 cut(s) 531, 599
AcoI YGGCCR 1 cut(s) 550
AfaI GTAC 2 cut(s) 221, 364
AfiI CCNNNNNNNGG 1 cut(s) 229
AgsI TTSAA 4 cut(s) 118, 375, 460, 598
AluBI AGCT 3 cut(s) 94, 217, 226
AluI AGCT 3 cut(s) 94, 217, 226
Alw26I GTCTC 6 cut(s) 161, 248, 272, 277, 485, 522
AlwI GGATC 2 cut(s) 531, 599
AlwNI CAGNNNCTG 1 cut(s) 187
AoxI GGCC 2 cut(s) 163, 550
Asp700I GAANNNNTTC 1 cut(s) 56
AspS9I GGNCC 1 cut(s) 302
AsuHPI GGTGA 1 cut(s) 592
AvaII GGWCC 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 477
BalI TGGCCA 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 227
BccI CCATC 3 cut(s) 71, 537, 601
BceAI ACGGC 1 cut(s) 337
BcoDI GTCTC 6 cut(s) 161, 248, 272, 277, 485, 522
BfaI CTAG 1 cut(s) 264
BglI GCCNNNNNGGC 1 cut(s) 84
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 382, 474
BmsI GCATC 2 cut(s) 166, 431
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 80
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 486
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 4 cut(s) 82, 151, 384, 576
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 396
Bse21I CCTNAGG 1 cut(s) 477
Bse3DI GCAATG 1 cut(s) 45
BseDI CCNNGG 4 cut(s) 82, 151, 384, 576
BseGI GGATG 2 cut(s) 548, 612
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 4 cut(s) 194, 218, 243, 468
BseNI ACTGG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 2 cut(s) 165, 552
BsiEI CGRYCG 1 cut(s) 76
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 6 cut(s) 161, 248, 272, 277, 485, 522
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 2 cut(s) 165, 552
Bso31I GGTCTC 1 cut(s) 161
Bsp143I GATC 4 cut(s) 6, 73, 523, 591
BspACI CCGC 1 cut(s) 79
BspANI GGCC 2 cut(s) 165, 552
BspCNI CTCAG 4 cut(s) 193, 219, 244, 469
BspLI GGNNCC 2 cut(s) 382, 474
BspPI GGATC 2 cut(s) 531, 599
BspTNI GGTCTC 1 cut(s) 161
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 396
BssECI CCNNGG 4 cut(s) 82, 151, 384, 576
BssMI GATC 4 cut(s) 6, 73, 523, 591
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 2 cut(s) 496, 533
Bst6I CTCTTC 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 4 cut(s) 180, 227, 252, 477
BstF5I GGATG 2 cut(s) 548, 612
BstKTI GATC 4 cut(s) 9, 76, 526, 594
BstMAI GTCTC 6 cut(s) 161, 248, 272, 277, 485, 522
BstMBI GATC 4 cut(s) 6, 73, 523, 591
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 6 cut(s) 35, 84, 176, 223, 419, 449
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 591
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 1 cut(s) 591
Bsu36I CCTNAGG 1 cut(s) 477
BsuRI GGCC 2 cut(s) 165, 552
BtgZI GCGATG 2 cut(s) 49, 467
BtsCI GGATG 2 cut(s) 548, 612
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 417, 525
Cac8I GCNNGC 1 cut(s) 415
CaiI CAGNNNCTG 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 220, 363
CviAII CATG 1 cut(s) 469
CviQI GTAC 2 cut(s) 220, 363
DdeI CTNAG 4 cut(s) 180, 227, 252, 477
DpnI GATC 4 cut(s) 8, 75, 525, 593
DpnII GATC 4 cut(s) 6, 73, 523, 591
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 1 cut(s) 472
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 1 cut(s) 415
FatI CATG 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 1 cut(s) 555
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 1 cut(s) 264
GluI GCNGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 2 cut(s) 165, 552
HapII CCGG 1 cut(s) 162
Hin1II CATG 1 cut(s) 472
HinfI GANTC 4 cut(s) 132, 184, 371, 428
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 592
Hpy188I TCNGA 5 cut(s) 73, 183, 195, 380, 427
Hpy188III TCNNGA 4 cut(s) 97, 118, 527, 595
HpyAV CCTTC 3 cut(s) 391, 577, 592
HpyCH4III ACNGT 2 cut(s) 496, 533
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 84, 176, 223, 419, 449
HpyF3I CTNAG 4 cut(s) 180, 227, 252, 477
Hsp92II CATG 1 cut(s) 472
Kzo9I GATC 4 cut(s) 6, 73, 523, 591
LmnI GCTCC 2 cut(s) 99, 380
LweI GCATC 2 cut(s) 166, 431
MaeI CTAG 1 cut(s) 264
MaeIII GTNAC 1 cut(s) 565
MalI GATC 4 cut(s) 8, 75, 525, 593
MboI GATC 4 cut(s) 6, 73, 523, 591
MboII GAAGA 4 cut(s) 61, 133, 245, 356
MflI RGATCY 1 cut(s) 591
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 1 cut(s) 482
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 3 cut(s) 141, 178, 437
Mox20I TGGCCA 1 cut(s) 552
MroXI GAANNNNTTC 1 cut(s) 56
MscI TGGCCA 1 cut(s) 552
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 1 cut(s) 162
MwoI GCNNNNNNNGC 6 cut(s) 35, 84, 176, 223, 419, 449
NdeII GATC 4 cut(s) 6, 73, 523, 591
NlaIII CATG 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 382, 474
NmeAIII GCCGAG 2 cut(s) 63, 254
NmuCI GTSAC 1 cut(s) 565
PdmI GAANNNNTTC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 1 cut(s) 80
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 3 cut(s) 140, 178, 436
PpsI GAGTC 3 cut(s) 140, 178, 436
PspN4I GGNNCC 2 cut(s) 382, 474
PspPI GGNCC 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 1 cut(s) 591
PvuI CGATCG 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 226
RsaI GTAC 2 cut(s) 221, 364
RsaNI GTAC 2 cut(s) 220, 363
RseI CAYNNNNRTG 1 cut(s) 558
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 4 cut(s) 6, 73, 523, 591
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 3 cut(s) 141, 178, 437
SetI ASST 7 cut(s) 28, 96, 219, 228, 299, 478, 582
SfaNI GCATC 2 cut(s) 166, 431
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 1 cut(s) 482
SsiI CCGC 1 cut(s) 79
SspMI CTAG 1 cut(s) 264
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 2 cut(s) 496, 533
TaqI TCGA 3 cut(s) 5, 12, 526
TaqII GACCGA 1 cut(s) 143
TasI AATT 1 cut(s) 482
TatI WGTACW 1 cut(s) 362
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 2 cut(s) 424, 525
TseFI GTSAC 1 cut(s) 565
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 4 cut(s) 260, 301, 346, 357
TspRI CASTG 2 cut(s) 424, 525
VpaK11BI GGWCC 1 cut(s) 302
XmnI GAANNNNTTC 1 cut(s) 56
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.