Rh6BG380500

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0003233
Physical Location & Seq
Reverse (-)
60875507 .. 60878309
2803 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG380500.1

Sequence Viewer

Length: 570 bp
ATGTCGATCTTCGAGTATAATGGAGGTGCTATAATCGCAATGGTGGGGAAGAACTGCTTCGCCATCGCCTCCGATCGCCGCCTCGGCGTTCAGCTCCAGACAATCGCAACCGACTTCAAGAAGATTTACAGAGTCAGCGATAGGCTCTTCCTCGGCCTCTCCGGCCTCGCCACCGATGCTCAGACTCTGTTTCAGAGGCTTATGTTTAAGCACAAGCTGTACCAGCTGAGGGAAGAGAGGGATATGAAGCCTGAGACTTTTGCTAGTCTCGTCTCGGCTATTCTCTACGAGAAAAGGTTCGGTCCATACTTCATTCAGCCTGTGATTGCTGGATTGGGAGATGAAGACAAGCCGTTCATCTGTACTATGGATTCAATCGGAGCCAAGGAACTGGCGAAGGATTTTGTTGTTGCTGGCACTGCATCAGAGTCCCTTTATGGTGCTTGTGAGGCAATGTTCAAGCCTGACATGGAACCTGAGGAATTATTTGAGACTGTATCTCAAGCACTTCTATCATCAGTGGATCGAGACTGTTTGAGTGGATGGGGTGGCCATATCTATGTTGTGTAA

Protein Analysis

189

Amino Acids

21.05

Weight (kDa)

5.12

Isoelectric Point (pI)

33.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome PF00227 7 - 189 2.9e-47 Proteasome subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 79
AclWI GGATC 1 cut(s) 531
AcoI YGGCCR 1 cut(s) 550
AfaI GTAC 2 cut(s) 221, 364
AfiI CCNNNNNNNGG 1 cut(s) 229
AgsI TTSAA 3 cut(s) 118, 375, 460
AluBI AGCT 3 cut(s) 94, 217, 226
AluI AGCT 3 cut(s) 94, 217, 226
Alw26I GTCTC 5 cut(s) 248, 272, 277, 485, 522
AlwI GGATC 1 cut(s) 531
AlwNI CAGNNNCTG 1 cut(s) 187
AoxI GGCC 3 cut(s) 154, 163, 550
Asp700I GAANNNNTTC 2 cut(s) 56, 296
AspS9I GGNCC 1 cut(s) 302
AvaII GGWCC 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 477
BalI TGGCCA 1 cut(s) 552
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 227
BccI CCATC 2 cut(s) 71, 537
BceAI ACGGC 1 cut(s) 337
BcoDI GTCTC 5 cut(s) 248, 272, 277, 485, 522
BfaI CTAG 1 cut(s) 264
BglI GCCNNNNNGGC 2 cut(s) 84, 162
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme18I GGWCC 1 cut(s) 302
BmgT120I GGNCC 1 cut(s) 302
BmiI GGNNCC 2 cut(s) 382, 474
BmsI GCATC 2 cut(s) 166, 431
BpiI GAAGAC 1 cut(s) 351
BpmI CTGGAG 1 cut(s) 80
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 486
BsaJI CCNNGG 3 cut(s) 82, 151, 384
Bsc4I CCNNNNNNNGG 1 cut(s) 229
Bse1I ACTGG 1 cut(s) 396
Bse21I CCTNAGG 1 cut(s) 477
Bse3DI GCAATG 2 cut(s) 45, 459
BseDI CCNNGG 3 cut(s) 82, 151, 384
BseGI GGATG 1 cut(s) 548
BseLI CCNNNNNNNGG 1 cut(s) 229
BseMI GCAATG 2 cut(s) 45, 459
BseMII CTCAG 4 cut(s) 194, 218, 243, 468
BseNI ACTGG 1 cut(s) 396
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 3 cut(s) 156, 165, 552
BsiEI CGRYCG 1 cut(s) 76
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 415
BslI CCNNNNNNNGG 1 cut(s) 229
BsmAI GTCTC 5 cut(s) 248, 272, 277, 485, 522
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 1 cut(s) 415
BsnI GGCC 3 cut(s) 156, 165, 552
Bsp143I GATC 3 cut(s) 6, 73, 523
BspACI CCGC 1 cut(s) 79
BspANI GGCC 3 cut(s) 156, 165, 552
BspCNI CTCAG 4 cut(s) 193, 219, 244, 469
BspLI GGNNCC 2 cut(s) 382, 474
BspPI GGATC 1 cut(s) 531
BspQI GCTCTTC 1 cut(s) 152
BsrDI GCAATG 2 cut(s) 45, 459
BsrI ACTGG 1 cut(s) 396
BssECI CCNNGG 3 cut(s) 82, 151, 384
BssMI GATC 3 cut(s) 6, 73, 523
BssT1I CCWWGG 1 cut(s) 384
Bst4CI ACNGT 2 cut(s) 496, 533
Bst6I CTCTTC 2 cut(s) 152, 228
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 4 cut(s) 180, 227, 252, 477
BstF5I GGATG 1 cut(s) 548
BstKTI GATC 3 cut(s) 9, 76, 526
BstMAI GTCTC 5 cut(s) 248, 272, 277, 485, 522
BstMBI GATC 3 cut(s) 6, 73, 523
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 7 cut(s) 35, 84, 162, 176, 223, 419, 449
BstV2I GAAGAC 1 cut(s) 351
BstXI CCANNNNNNTGG 1 cut(s) 391
Bsu36I CCTNAGG 1 cut(s) 477
BsuRI GGCC 3 cut(s) 156, 165, 552
BtgZI GCGATG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 548
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 2 cut(s) 417, 525
Cac8I GCNNGC 1 cut(s) 415
CaiI CAGNNNCTG 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 302
Csp6I GTAC 2 cut(s) 220, 363
CviAII CATG 1 cut(s) 469
CviQI GTAC 2 cut(s) 220, 363
DdeI CTNAG 4 cut(s) 180, 227, 252, 477
DpnI GATC 3 cut(s) 8, 75, 525
DpnII GATC 3 cut(s) 6, 73, 523
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 2 cut(s) 152, 228
EarI CTCTTC 2 cut(s) 152, 228
Eco130I CCWWGG 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 477
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 1 cut(s) 472
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 1 cut(s) 415
FatI CATG 1 cut(s) 468
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 1 cut(s) 555
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 1 cut(s) 264
GluI GCNGC 1 cut(s) 79
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 3 cut(s) 156, 165, 552
HapII CCGG 1 cut(s) 162
Hin1II CATG 1 cut(s) 472
HinfI GANTC 4 cut(s) 132, 184, 371, 428
HpaII CCGG 1 cut(s) 162
Hpy188I TCNGA 5 cut(s) 73, 183, 195, 380, 427
Hpy188III TCNNGA 3 cut(s) 97, 118, 527
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 2 cut(s) 496, 533
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 84, 162, 176, 223, 419, 449
HpyF3I CTNAG 4 cut(s) 180, 227, 252, 477
Hsp92II CATG 1 cut(s) 472
Kzo9I GATC 3 cut(s) 6, 73, 523
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 2 cut(s) 99, 380
LweI GCATC 2 cut(s) 166, 431
MaeI CTAG 1 cut(s) 264
MalI GATC 3 cut(s) 8, 75, 525
MboI GATC 3 cut(s) 6, 73, 523
MboII GAAGA 5 cut(s) 61, 133, 139, 245, 356
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 1 cut(s) 482
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 3 cut(s) 141, 178, 437
Mox20I TGGCCA 1 cut(s) 552
MroXI GAANNNNTTC 2 cut(s) 56, 296
MscI TGGCCA 1 cut(s) 552
MseI TTAA 1 cut(s) 207
MslI CAYNNNNRTG 1 cut(s) 558
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 1 cut(s) 162
MwoI GCNNNNNNNGC 7 cut(s) 35, 84, 162, 176, 223, 419, 449
NdeII GATC 3 cut(s) 6, 73, 523
NlaIII CATG 1 cut(s) 472
NlaIV GGNNCC 2 cut(s) 382, 474
NmeAIII GCCGAG 3 cut(s) 63, 132, 254
PciSI GCTCTTC 1 cut(s) 152
PdmI GAANNNNTTC 2 cut(s) 56, 296
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 1 cut(s) 80
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 3 cut(s) 140, 178, 436
PpsI GAGTC 3 cut(s) 140, 178, 436
PspN4I GGNNCC 2 cut(s) 382, 474
PspPI GGNCC 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 187
PvuI CGATCG 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 226
RsaI GTAC 2 cut(s) 221, 364
RsaNI GTAC 2 cut(s) 220, 363
RseI CAYNNNNRTG 1 cut(s) 558
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 3 cut(s) 6, 73, 523
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 3 cut(s) 141, 178, 437
SetI ASST 6 cut(s) 28, 96, 219, 228, 299, 478
SfaNI GCATC 2 cut(s) 166, 431
SfiI GGCCNNNNNGGCC 1 cut(s) 162
SinI GGWCC 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 558
SmlI CTYRAG 1 cut(s) 501
SmoI CTYRAG 1 cut(s) 501
Sse9I AATT 1 cut(s) 482
SsiI CCGC 1 cut(s) 79
SspMI CTAG 1 cut(s) 264
StyI CCWWGG 1 cut(s) 384
TaaI ACNGT 2 cut(s) 496, 533
TaqI TCGA 3 cut(s) 5, 12, 526
TaqII GACCGA 1 cut(s) 290
TasI AATT 1 cut(s) 482
TatI WGTACW 1 cut(s) 362
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 2 cut(s) 424, 525
TspDTI ATGAA 4 cut(s) 260, 301, 346, 357
TspRI CASTG 2 cut(s) 424, 525
VpaK11BI GGWCC 1 cut(s) 302
XmnI GAANNNNTTC 2 cut(s) 56, 296
XspI CTAG 1 cut(s) 264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.