Rmu_sc0022551.1_g000001

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0022551.1
Physical Location & Seq
Forward (+)
186 .. 2275
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0022551.1_g000001.1.cds

Sequence Viewer

Length: 687 bp
atggctggtgatactgctgtaaggtcattgctagaaaagatggctgaatgtgctagcaatgcttacctgggtatattagagaggtgggtctatgaaggagtcatcgatgatccttatggtgaatttttcattgcggagaacaaatctctgcaaaaggaaagcctcactcaagattatgatgccaagtattggaggcaacgttacagccttaaggatggaattcctagctttcttgcaaatattgctggaacaattttgatcaccggaaagtatctaaatgtcatgagagaatgcgggcaccatgttcaggtccctgtatctgaaaattcaaaattgatgagctttggatcaaatcatcgttatatcgaatgtataaaatctgcttatgactttgcaagcagcgagctcttaaatcttatcaaagaaaagtatgatctgatgggaaagctacgatctataaagcactatcttcttcttgatcagggtgactttttagttcattttatggatattgctcgggatgagcttatgaaaaagcttgacgacatttctgtggagaaactgcaatctctactagaccttgctttgcgaactacagcagctgcagcagatccctgccatgaggacttaacatgttgtgtggtaaacagctcgaaagttttgcttctatgtgaaaagcatggataa

Protein Analysis

228

Amino Acids

25.83

Weight (kDa)

5.52

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 297
AccB7I CCANNNNNTGG 1 cut(s) 189
AciI CCGC 2 cut(s) 134, 294
AclI AACGTT 1 cut(s) 199
AclWI GGATC 3 cut(s) 104, 355, 605
AcsI RAATTY 3 cut(s) 122, 219, 325
AfiI CCNNNNNNNGG 2 cut(s) 189, 307
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 632
AgsI TTSAA 1 cut(s) 330
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 8 cut(s) 228, 342, 406, 448, 526, 538, 602, 651
AluI AGCT 8 cut(s) 228, 342, 406, 448, 526, 538, 602, 651
Alw21I GWGCWC 1 cut(s) 408
AlwI GGATC 3 cut(s) 104, 355, 605
AlwNI CAGNNNCTG 1 cut(s) 602
Ama87I CYCGRG 1 cut(s) 516
ApeKI GCWGC 4 cut(s) 399, 599, 602, 605
ApoI RAATTY 3 cut(s) 122, 219, 325
AspS9I GGNCC 1 cut(s) 310
AsuHPI GGTGA 4 cut(s) 20, 131, 253, 497
AsuNHI GCTAGC 1 cut(s) 53
AvaI CYCGRG 1 cut(s) 516
AvaII GGWCC 1 cut(s) 310
BaeGI GKGCMC 1 cut(s) 300
BaeI ACNNNNGTAYC 1 cut(s) 36
BanI GGYRCC 1 cut(s) 297
BanII GRGCYC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 408
BbvI GCAGC 4 cut(s) 411, 589, 611, 617
BccI CCATC 3 cut(s) 34, 209, 433
BcgI CGANNNNNNTGC 2 cut(s) 643, 677
BciT130I CCWGG 1 cut(s) 68
BclI TGATCA 2 cut(s) 258, 478
BfaI CTAG 4 cut(s) 32, 54, 225, 575
BfmI CTRYAG 2 cut(s) 594, 603
BfrI CTTAAG 1 cut(s) 209
BisI GCNGC 4 cut(s) 400, 600, 603, 606
BlsI GCNGC 4 cut(s) 401, 601, 604, 607
Bme1390I CCNGG 1 cut(s) 68
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 516
BmgT120I GGNCC 1 cut(s) 310
BmiI GGNNCC 2 cut(s) 299, 312
BmrFI CCNGG 1 cut(s) 68
BmsI GCATC 1 cut(s) 169
BmtI GCTAGC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 153
Bsa29I ATCGAT 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 263
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 307
Bse3DI GCAATG 3 cut(s) 26, 64, 129
BseBI CCWGG 1 cut(s) 68
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 2 cut(s) 220, 526
BseLI CCNNNNNNNGG 2 cut(s) 189, 307
BseMI GCAATG 3 cut(s) 26, 64, 129
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 4 cut(s) 411, 589, 611, 617
BshNI GGYRCC 1 cut(s) 297
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 516
BsiSI CCGG 1 cut(s) 264
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 2 cut(s) 189, 307
BsmFI GGGAC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 296
BsoBI CYCGRG 1 cut(s) 516
Bsp1286I GDGCHC 2 cut(s) 300, 408
Bsp143I GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
BspACI CCGC 2 cut(s) 134, 294
BspDI ATCGAT 1 cut(s) 105
BspHI TCATGA 1 cut(s) 282
BspLI GGNNCC 2 cut(s) 299, 312
BspMAI CTGCAG 1 cut(s) 607
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 3 cut(s) 104, 355, 605
BspT107I GGYRCC 1 cut(s) 297
BspTI CTTAAG 1 cut(s) 209
BsrDI GCAATG 3 cut(s) 26, 64, 129
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
Bst2UI CCWGG 1 cut(s) 68
BstAFI CTTAAG 1 cut(s) 209
BstAPI GCANNNNNTGC 1 cut(s) 242
BstC8I GCNNGC 4 cut(s) 55, 296, 397, 404
BstF5I GGATG 2 cut(s) 220, 526
BstKTI GATC 7 cut(s) 112, 261, 350, 436, 455, 481, 613
BstMBI GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
BstMWI GCNNNNNNNGC 4 cut(s) 50, 59, 242, 605
BstNI CCWGG 1 cut(s) 68
BstNSI RCATGY 1 cut(s) 636
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 2 cut(s) 594, 603
BstSLI GKGCMC 1 cut(s) 300
BstV1I GCAGC 4 cut(s) 411, 589, 611, 617
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
Bsu15I ATCGAT 1 cut(s) 105
BsuTUI ATCGAT 1 cut(s) 105
BtsCI GGATG 2 cut(s) 220, 526
Cac8I GCNNGC 4 cut(s) 55, 296, 397, 404
CaiI CAGNNNCTG 1 cut(s) 602
CciI TCATGA 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 310
ClaI ATCGAT 1 cut(s) 105
CviAII CATG 5 cut(s) 283, 302, 620, 633, 680
DpnI GATC 7 cut(s) 111, 260, 349, 435, 454, 480, 612
DpnII GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
Ecl136II GAGCTC 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 408
Eco47I GGWCC 1 cut(s) 310
Eco53kI GAGCTC 1 cut(s) 406
Eco88I CYCGRG 1 cut(s) 516
EcoICRI GAGCTC 1 cut(s) 406
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 66
EcoT38I GRGCYC 1 cut(s) 408
FaeI CATG 5 cut(s) 286, 305, 623, 636, 683
FalI AAGNNNNNCTT 2 cut(s) 648, 680
FaqI GGGAC 1 cut(s) 296
FatI CATG 5 cut(s) 282, 301, 619, 632, 679
FauI CCCGC 1 cut(s) 287
FbaI TGATCA 2 cut(s) 258, 478
Fnu4HI GCNGC 4 cut(s) 400, 600, 603, 606
FokI GGATG 2 cut(s) 227, 533
FriOI GRGCYC 1 cut(s) 408
Fsp4HI GCNGC 4 cut(s) 400, 600, 603, 606
FspBI CTAG 4 cut(s) 32, 54, 225, 575
GluI GCNGC 4 cut(s) 400, 600, 603, 606
HapII CCGG 1 cut(s) 264
Hin1II CATG 5 cut(s) 286, 305, 623, 636, 683
HindIII AAGCTT 1 cut(s) 536
HinfI GANTC 1 cut(s) 99
HpaII CCGG 1 cut(s) 264
HphI GGTGA 4 cut(s) 20, 131, 253, 497
Hpy166II GTNNAC 1 cut(s) 646
Hpy188I TCNGA 2 cut(s) 322, 438
Hpy188III TCNNGA 4 cut(s) 170, 283, 476, 518
Hpy8I GTNNAC 1 cut(s) 646
HpyAV CCTTC 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 5 cut(s) 151, 236, 395, 565, 605
HpyF10VI GCNNNNNNNGC 4 cut(s) 50, 59, 242, 605
HpySE526I ACGT 1 cut(s) 199
Hsp92II CATG 5 cut(s) 286, 305, 623, 636, 683
Ksp22I TGATCA 2 cut(s) 258, 478
Kzo9I GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
LpnPI CCDG 8 cut(s) 53, 80, 231, 277, 293, 327, 467, 628
Lsp1109I GCAGC 4 cut(s) 411, 589, 611, 617
LweI GCATC 1 cut(s) 169
MaeI CTAG 4 cut(s) 32, 54, 225, 575
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 2 cut(s) 200, 485
MalI GATC 7 cut(s) 111, 260, 349, 435, 454, 480, 612
MboI GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
MboII GAAGA 2 cut(s) 461, 464
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 2 cut(s) 300, 408
MluCI AATT 5 cut(s) 122, 219, 252, 325, 332
MlyI GAGTC 1 cut(s) 108
MnlI CCTC 4 cut(s) 75, 173, 186, 616
MseI TTAA 3 cut(s) 210, 410, 629
MslI CAYNNNNRTG 1 cut(s) 551
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 68
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 4 cut(s) 50, 59, 242, 605
NdeII GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
NheI GCTAGC 1 cut(s) 53
NlaIII CATG 5 cut(s) 286, 305, 623, 636, 683
NlaIV GGNNCC 2 cut(s) 299, 312
NmuCI GTSAC 1 cut(s) 485
NspI RCATGY 1 cut(s) 636
PagI TCATGA 1 cut(s) 282
PciI ACATGT 1 cut(s) 632
PctI GAATGC 1 cut(s) 296
PflMI CCANNNNNTGG 1 cut(s) 189
PkrI GCNGC 4 cut(s) 401, 601, 604, 607
PleI GAGTC 1 cut(s) 107
PpsI GAGTC 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 310
PscI ACATGT 1 cut(s) 632
Psp124BI GAGCTC 1 cut(s) 408
Psp1406I AACGTT 1 cut(s) 199
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspN4I GGNNCC 2 cut(s) 299, 312
PspPI GGNCC 1 cut(s) 310
PspPPI RGGWCCY 1 cut(s) 310
PstI CTGCAG 1 cut(s) 607
PstNI CAGNNNCTG 1 cut(s) 602
PsuI RGATCY 1 cut(s) 610
PvuII CAGCTG 1 cut(s) 602
RseI CAYNNNNRTG 1 cut(s) 551
SacI GAGCTC 1 cut(s) 408
SaqAI TTAA 3 cut(s) 210, 410, 629
SatI GCNGC 4 cut(s) 400, 600, 603, 606
Sau3AI GATC 7 cut(s) 109, 258, 347, 433, 452, 478, 610
Sau96I GGNCC 1 cut(s) 310
SchI GAGTC 1 cut(s) 108
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 2 cut(s) 300, 408
SfaNI GCATC 1 cut(s) 169
SfcI CTRYAG 2 cut(s) 594, 603
SinI GGWCC 1 cut(s) 310
SmiMI CAYNNNNRTG 1 cut(s) 551
SmlI CTYRAG 2 cut(s) 168, 209
SmoI CTYRAG 2 cut(s) 168, 209
Sse9I AATT 5 cut(s) 122, 219, 252, 325, 332
SsiI CCGC 2 cut(s) 134, 294
SspI AATATT 1 cut(s) 241
SspMI CTAG 4 cut(s) 32, 54, 225, 575
SstI GAGCTC 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 66
TaiI ACGT 1 cut(s) 202
TaqI TCGA 3 cut(s) 105, 366, 653
TasI AATT 5 cut(s) 122, 219, 252, 325, 332
Tru1I TTAA 3 cut(s) 210, 410, 629
Tru9I TTAA 3 cut(s) 210, 410, 629
TseFI GTSAC 1 cut(s) 485
TseI GCWGC 4 cut(s) 399, 599, 602, 605
Tsp45I GTSAC 1 cut(s) 485
TspDTI ATGAA 4 cut(s) 108, 118, 488, 545
Van91I CCANNNNNTGG 1 cut(s) 189
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 3 cut(s) 122, 219, 325
XceI RCATGY 1 cut(s) 636
XspI CTAG 4 cut(s) 32, 54, 225, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.