RLG00000019374

Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003233
Physical Location & Seq
Forward (+)
51083923 .. 51086406
2484 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019374

Sequence Viewer

Length: 423 bp
ATGTTGGCGATGGAGATCAATAGTTCAATTTCGTGCCCTTCAACTCCTTGTTGGATCTCCGAGAGGCCGTTCCTCACTGATCGCTTCCATCAGTTTGTGCAGAAAACCAATGGACGTCGCGTTTCGCCACCGATACCAAGGATTCCCATACTGTGGGATTCCAAGATTCCATTGGCTGTTACAATGCTGCTGTTTAGGCTAAGGCCATGGCTGGTGATACTGCTGTGGGTATATGAAGGAGTCATTGATGATCCTTACGGTGAATTTTTCATTGCTGAGAACAAATCTCTTCAAAAGGAAAGCCTCAAGATTATGAAGCCAAGTTCCACTATGGTTTATCCTTATGATCTGATAGGGAAGCTACGATCTATAAAGCACTATCTTCTTCTTGATCAGGTATATATACTGCTGTATGACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000226 GO:0000278 GO:0000280 GO:0000923 GO:0000930 GO:0003674 GO:0005198 GO:0005200 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005815 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006996 GO:0007010 GO:0007017 GO:0007020 GO:0007049 GO:0007051 GO:0007052 GO:0007275 GO:0008092 GO:0008104 GO:0008150 GO:0008275 GO:0009987 GO:0010638 GO:0010968 GO:0012505 GO:0015630 GO:0015631 GO:0016043 GO:0022402 GO:0022414 GO:0022607 GO:0030863 GO:0030981 GO:0031109 GO:0031110 GO:0031112 GO:0031113 GO:0031116 GO:0031122 GO:0031334 GO:0031503 GO:0031967 GO:0031975 GO:0032153 GO:0032155 GO:0032271 GO:0032273 GO:0032501 GO:0032502 GO:0032886 GO:0032991 GO:0033036 GO:0033043 GO:0033566 GO:0034613 GO:0034622 GO:0034629 GO:0043015 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044450 GO:0044464 GO:0046785 GO:0048229 GO:0048285 GO:0048518 GO:0048522 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051179 GO:0051225 GO:0051258 GO:0051321 GO:0051415 GO:0051418 GO:0051493 GO:0051495 GO:0051641 GO:0055028 GO:0065003 GO:0065007 GO:0070507 GO:0070727 GO:0070925 GO:0071840 GO:0071944 GO:0090063 GO:0090307 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0140014 GO:1902850 GO:1902903 GO:1902905 GO:1903047
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spc97_Spc98

Protein Analysis

141

Amino Acids

16.45

Weight (kDa)

8.91

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCP_N_terminal PF17681 69 - 103 1.5e-07 Gamma tubulin complex component N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 118
AccB7I CCANNNNNTGG 1 cut(s) 153
AccII CGCG 1 cut(s) 120
AclWI GGATC 2 cut(s) 62, 245
AcsI RAATTY 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 115
AfiI CCNNNNNNNGG 1 cut(s) 153
AgsI TTSAA 3 cut(s) 27, 42, 293
AluBI AGCT 1 cut(s) 361
AluI AGCT 1 cut(s) 361
AlwI GGATC 2 cut(s) 62, 245
AoxI GGCC 2 cut(s) 65, 203
ApeKI GCWGC 1 cut(s) 187
ApoI RAATTY 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 226, 272
BaeGI GKGCMC 1 cut(s) 38
BaeI ACNNNNGTAYC 2 cut(s) 209, 242
BbvI GCAGC 1 cut(s) 174
BccI CCATC 2 cut(s) 4, 96
BceAI ACGGC 1 cut(s) 52
BclI TGATCA 1 cut(s) 391
BisI GCNGC 1 cut(s) 188
BlsI GCNGC 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 290
BsaBI GATNNNNATC 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 115
BsaJI CCNNGG 2 cut(s) 137, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 270
Bse8I GATNNNNATC 1 cut(s) 14
BseDI CCNNGG 2 cut(s) 137, 206
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 153
BseMI GCAATG 1 cut(s) 270
BseMII CTCAG 1 cut(s) 267
BseNI ACTGG 1 cut(s) 418
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 1 cut(s) 174
BsgI GTGCAG 1 cut(s) 119
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 2 cut(s) 67, 205
BslI CCNNNNNNNGG 1 cut(s) 153
BsnI GGCC 2 cut(s) 67, 205
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
Bsp19I CCATGG 1 cut(s) 206
BspANI GGCC 2 cut(s) 67, 205
BspCNI CTCAG 1 cut(s) 268
BspFNI CGCG 1 cut(s) 120
BspPI GGATC 2 cut(s) 62, 245
BsrDI GCAATG 1 cut(s) 270
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 137, 206
BssMI GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
BssNI GRCGYC 1 cut(s) 115
BssT1I CCWWGG 2 cut(s) 137, 206
Bst4CI ACNGT 2 cut(s) 153, 260
Bst6I CTCTTC 1 cut(s) 294
BstACI GRCGYC 1 cut(s) 115
BstDEI CTNAG 2 cut(s) 200, 276
BstDSI CCRYGG 1 cut(s) 206
BstFNI CGCG 1 cut(s) 120
BstKTI GATC 7 cut(s) 18, 57, 82, 253, 349, 368, 394
BstMBI GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 1 cut(s) 174
BstX2I RGATCY 1 cut(s) 54
BstYI RGATCY 1 cut(s) 54
BsuRI GGCC 2 cut(s) 67, 205
BtgI CCRYGG 1 cut(s) 206
BtgZI GCGATG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 75
CviAII CATG 1 cut(s) 207
CviJI RGCY 8 cut(s) 67, 176, 199, 205, 211, 303, 319, 361
CviKI_1 RGCY 8 cut(s) 67, 176, 199, 205, 211, 303, 319, 361
DdeI CTNAG 2 cut(s) 200, 276
DpnI GATC 7 cut(s) 17, 56, 81, 252, 348, 367, 393
DpnII GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 2 cut(s) 137, 206
EcoT14I CCWWGG 2 cut(s) 137, 206
ErhI CCWWGG 2 cut(s) 137, 206
FaeI CATG 1 cut(s) 210
FatI CATG 1 cut(s) 206
FbaI TGATCA 1 cut(s) 391
Fnu4HI GCNGC 1 cut(s) 188
Fsp4HI GCNGC 1 cut(s) 188
GluI GCNGC 1 cut(s) 188
HaeIII GGCC 2 cut(s) 67, 205
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 1 cut(s) 210
HinfI GANTC 4 cut(s) 142, 158, 166, 240
HphI GGTGA 2 cut(s) 226, 272
Hpy188I TCNGA 2 cut(s) 61, 351
Hpy188III TCNNGA 2 cut(s) 307, 389
Hpy99I CGWCG 1 cut(s) 120
HpyAV CCTTC 2 cut(s) 48, 230
HpyCH4III ACNGT 2 cut(s) 153, 260
HpyCH4IV ACGT 1 cut(s) 115
HpyCH4V TGCA 1 cut(s) 100
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 2 cut(s) 200, 276
HpySE526I ACGT 1 cut(s) 115
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 1 cut(s) 210
Ksp22I TGATCA 1 cut(s) 391
Kzo9I GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
LpnPI CCDG 2 cut(s) 197, 380
Lsp1109I GCAGC 1 cut(s) 174
MaeII ACGT 1 cut(s) 115
MaeIII GTNAC 1 cut(s) 178
MalI GATC 7 cut(s) 17, 56, 81, 252, 348, 367, 393
MboI GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
MboII GAAGA 3 cut(s) 281, 374, 377
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 2 cut(s) 27, 263
MlyI GAGTC 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 3 cut(s) 57, 83, 314
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 196
NcoI CCATGG 1 cut(s) 206
NdeII GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
NlaIII CATG 1 cut(s) 210
PfeI GAWTC 3 cut(s) 142, 158, 166
PflMI CCANNNNNTGG 1 cut(s) 153
PkrI GCNGC 1 cut(s) 189
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
PsuI RGATCY 1 cut(s) 54
SatI GCNGC 1 cut(s) 188
Sau3AI GATC 7 cut(s) 15, 54, 79, 250, 346, 365, 391
SchI GAGTC 1 cut(s) 249
SduI GDGCHC 1 cut(s) 38
SetI ASST 3 cut(s) 118, 363, 399
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 2 cut(s) 27, 263
StyI CCWWGG 2 cut(s) 137, 206
TaaI ACNGT 2 cut(s) 153, 260
TaiI ACGT 1 cut(s) 118
TasI AATT 2 cut(s) 27, 263
TfiI GAWTC 3 cut(s) 142, 158, 166
TscAI CASTG 1 cut(s) 82
TseI GCWGC 1 cut(s) 187
TspDTI ATGAA 3 cut(s) 249, 259, 329
TspRI CASTG 1 cut(s) 82
Van91I CCANNNNNTGG 1 cut(s) 153
XapI RAATTY 1 cut(s) 263
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
ZraI GACGTC 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.