FvH4_3g08421

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001932
Physical Location & Seq
Forward (+)
4919835 .. 4920924
1090 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08421.t1

Sequence Viewer

Length: 714 bp
ATGAGTTTCGAGGAGGAGTACATAGATGTGTTTTTAGTCCCAGCTGGGCTACTGATCATGCTAGTCTACCACCTCTTCCTCCTCTTCAAATACATCAATAAGCCTCTCTCCACATCCATAGGTTACGAAAACAACGACAAACGAATTTGGGTTGGAAAAATCTTGCAGTTGCAGGGTGATGATCTTAGAAAGAGTGTTAAAACAGCTCTCAATGTGCTTTCCTCGAGTACAACTGCAACTATTTACCTGGCCACAATCTCATTAACCCTCTGCTCACTTATTGGAGCTTGGATGGCAAACAACACAACCAATTTCTTCCCGAGAGAACTAATATATGGTAACACAAGTGCGTCGATTGTTTCGATCAAGTACATATGCCTCTTGTCCTGTTTTCTCCTTGGTTTTTCCTGCTTTGTTCAGTCAGCTAGGAACTTCGTTCATTCGAGCTTCCTATTAAGCACCCCCGGAGTTGCAGCAAAATCTGATGCAAAGAAAGTGGAGAGAGCGGTTCAAAGAGGAAGTGAACTTTGCTTACTAGGGCTTCGAGCTCTTTATTGTGCTCTTAATTTTCTCTTATGGTTTTTTGGACCTATACCAATGTTTGTTTCCTCTATAGTAATGGTGTTGATTCTTGGTTGTCATGACTTTAGATCAGATAATCATCAAAAGGCCGGCCTTAAAGATCAGCATCAAGAGAAACTGAATGCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

238

Amino Acids

26.48

Weight (kDa)

8.81

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 12 - 219 1.1e-53 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 66
AciI CCGC 1 cut(s) 506
AcoI YGGCCR 1 cut(s) 249
AcsI RAATTY 1 cut(s) 144
AfaI GTAC 3 cut(s) 20, 229, 371
AgsI TTSAA 2 cut(s) 88, 512
AjnI CCWGG 1 cut(s) 246
AjuI GAANNNNNNNTTGG 2 cut(s) 589, 621
AluBI AGCT 6 cut(s) 44, 206, 287, 425, 447, 548
AluI AGCT 6 cut(s) 44, 206, 287, 425, 447, 548
Alw21I GWGCWC 2 cut(s) 550, 562
Ama87I CYCGRG 2 cut(s) 223, 319
AoxI GGCC 3 cut(s) 249, 669, 673
ApeKI GCWGC 1 cut(s) 473
ApoI RAATTY 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 587
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 1 cut(s) 188
AvaI CYCGRG 2 cut(s) 223, 319
AvaII GGWCC 1 cut(s) 587
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 1 cut(s) 550
Bbv12I GWGCWC 2 cut(s) 550, 562
BbvI GCAGC 1 cut(s) 485
BccI CCATC 1 cut(s) 286
BciT130I CCWGG 1 cut(s) 248
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 1 cut(s) 465
BfaI CTAG 3 cut(s) 62, 426, 536
BfmI CTRYAG 1 cut(s) 612
BisI GCNGC 1 cut(s) 474
BlsI GCNGC 1 cut(s) 475
Bme1390I CCNGG 2 cut(s) 248, 465
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 2 cut(s) 223, 319
BmgT120I GGNCC 1 cut(s) 587
BmrFI CCNGG 2 cut(s) 248, 465
BmsI GCATC 2 cut(s) 475, 697
BpuMI CCSGG 1 cut(s) 465
BsaBI GATNNNNATC 2 cut(s) 660, 687
BsaJI CCNNGG 2 cut(s) 397, 463
Bse118I RCCGGY 1 cut(s) 671
Bse8I GATNNNNATC 2 cut(s) 660, 687
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 2 cut(s) 397, 463
BseGI GGATG 2 cut(s) 113, 297
BseJI GATNNNNATC 2 cut(s) 660, 687
BseRI GAGGAG 3 cut(s) 26, 29, 71
BseXI GCAGC 1 cut(s) 485
BseYI CCCAGC 2 cut(s) 40, 44
BshFI GGCC 3 cut(s) 251, 671, 675
BsiHKAI GWGCWC 2 cut(s) 550, 562
BsiHKCI CYCGRG 2 cut(s) 223, 319
BsiSI CCGG 2 cut(s) 465, 672
BslFI GGGAC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 23
BsmI GAATGC 1 cut(s) 709
BsnI GGCC 3 cut(s) 251, 671, 675
BsoBI CYCGRG 2 cut(s) 223, 319
Bsp1286I GDGCHC 2 cut(s) 550, 562
Bsp143I GATC 5 cut(s) 54, 181, 363, 650, 682
BspACI CCGC 1 cut(s) 506
BspANI GGCC 3 cut(s) 251, 671, 675
BspHI TCATGA 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 506
BsrFI RCCGGY 1 cut(s) 671
BssAI RCCGGY 1 cut(s) 671
BssECI CCNNGG 2 cut(s) 397, 463
BssMI GATC 5 cut(s) 54, 181, 363, 650, 682
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 248
Bst6I CTCTTC 2 cut(s) 80, 89
BstC8I GCNNGC 1 cut(s) 673
BstDEI CTNAG 1 cut(s) 185
BstF5I GGATG 2 cut(s) 113, 297
BstKTI GATC 5 cut(s) 57, 184, 366, 653, 685
BstMBI GATC 5 cut(s) 54, 181, 363, 650, 682
BstMWI GCNNNNNNNGC 1 cut(s) 293
BstNI CCWGG 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 246, 463
BstSFI CTRYAG 1 cut(s) 612
BstV1I GCAGC 1 cut(s) 485
BsuRI GGCC 3 cut(s) 251, 671, 675
BtsCI GGATG 2 cut(s) 113, 297
Cac8I GCNNGC 1 cut(s) 673
CciI TCATGA 1 cut(s) 640
Cfr10I RCCGGY 1 cut(s) 671
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 1 cut(s) 339
Csp6I GTAC 3 cut(s) 19, 228, 370
CspCI CAANNNNNGTGG 2 cut(s) 477, 512
CviAII CATG 2 cut(s) 58, 641
CviQI GTAC 3 cut(s) 19, 228, 370
DdeI CTNAG 1 cut(s) 185
DpnI GATC 5 cut(s) 56, 183, 365, 652, 684
DpnII GATC 5 cut(s) 54, 181, 363, 650, 682
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 2 cut(s) 80, 89
EarI CTCTTC 2 cut(s) 80, 89
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 397
Eco24I GRGCYC 1 cut(s) 550
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 548
Eco88I CYCGRG 2 cut(s) 223, 319
EcoICRI GAGCTC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 2 cut(s) 61, 644
FaqI GGGAC 1 cut(s) 23
FatI CATG 2 cut(s) 57, 640
FauNDI CATATG 1 cut(s) 374
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 474
FokI GGATG 2 cut(s) 100, 304
FriOI GRGCYC 1 cut(s) 550
FseI GGCCGGCC 1 cut(s) 675
Fsp4HI GCNGC 1 cut(s) 474
FspBI CTAG 3 cut(s) 62, 426, 536
GluI GCNGC 1 cut(s) 474
GsaI CCCAGC 2 cut(s) 44, 48
HaeIII GGCC 3 cut(s) 251, 671, 675
HapII CCGG 2 cut(s) 465, 672
HgaI GACGC 1 cut(s) 339
Hin1II CATG 2 cut(s) 61, 644
HinfI GANTC 1 cut(s) 628
HpaII CCGG 2 cut(s) 465, 672
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 67, 524
Hpy188I TCNGA 2 cut(s) 484, 655
Hpy188III TCNNGA 3 cut(s) 319, 641, 692
Hpy8I GTNNAC 2 cut(s) 67, 524
Hpy99I CGWCG 1 cut(s) 355
HpyCH4V TGCA 6 cut(s) 166, 172, 236, 473, 488, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 293
HpyF3I CTNAG 1 cut(s) 185
Hsp92II CATG 2 cut(s) 61, 644
KroI GCCGGC 1 cut(s) 671
KroNI GCCGGC 1 cut(s) 673
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 5 cut(s) 54, 181, 363, 650, 682
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 9 cut(s) 30, 54, 158, 233, 260, 400, 421, 478, 685
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 2 cut(s) 475, 697
MaeI CTAG 3 cut(s) 62, 426, 536
MaeIII GTNAC 2 cut(s) 122, 338
MalI GATC 5 cut(s) 56, 183, 365, 652, 684
MbiI CCGCTC 1 cut(s) 506
MboI GATC 5 cut(s) 54, 181, 363, 650, 682
MboII GAAGA 3 cut(s) 67, 76, 307
MhlI GDGCHC 2 cut(s) 550, 562
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 144, 310, 565
MluNI TGGCCA 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 133
Mox20I TGGCCA 1 cut(s) 251
MroNI GCCGGC 1 cut(s) 671
MscI TGGCCA 1 cut(s) 251
MseI TTAA 6 cut(s) 198, 263, 455, 564, 678, 712
MslI CAYNNNNRTG 1 cut(s) 26
Msp20I TGGCCA 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 2 cut(s) 465, 672
MspR9I CCNGG 2 cut(s) 248, 465
Mva1269I GAATGC 1 cut(s) 709
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 293
NaeI GCCGGC 1 cut(s) 673
NciI CCSGG 1 cut(s) 465
NdeI CATATG 1 cut(s) 374
NdeII GATC 5 cut(s) 54, 181, 363, 650, 682
NgoMIV GCCGGC 1 cut(s) 671
NlaIII CATG 2 cut(s) 61, 644
PaeR7I CTCGAG 1 cut(s) 223
PagI TCATGA 1 cut(s) 640
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 359
PctI GAATGC 1 cut(s) 709
PdiI GCCGGC 1 cut(s) 673
PfeI GAWTC 1 cut(s) 628
PkrI GCNGC 1 cut(s) 475
Psp124BI GAGCTC 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 246
PspFI CCCAGC 2 cut(s) 40, 44
PspGI CCWGG 1 cut(s) 246
PspPI GGNCC 1 cut(s) 587
PspXI VCTCGAGB 1 cut(s) 223
PsrI GAACNNNNNNTAC 2 cut(s) 516, 548
PvuII CAGCTG 1 cut(s) 44
RigI GGCCGGCC 1 cut(s) 675
RsaI GTAC 3 cut(s) 20, 229, 371
RsaNI GTAC 3 cut(s) 19, 228, 370
RseI CAYNNNNRTG 1 cut(s) 26
SacI GAGCTC 1 cut(s) 550
SaqAI TTAA 6 cut(s) 198, 263, 455, 564, 678, 712
SatI GCNGC 1 cut(s) 474
Sau3AI GATC 5 cut(s) 54, 181, 363, 650, 682
Sau96I GGNCC 1 cut(s) 587
ScrFI CCNGG 2 cut(s) 248, 465
SduI GDGCHC 2 cut(s) 550, 562
SfaNI GCATC 2 cut(s) 475, 697
SfcI CTRYAG 1 cut(s) 612
Sfr274I CTCGAG 1 cut(s) 223
SinI GGWCC 1 cut(s) 587
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 26
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 3 cut(s) 144, 310, 565
SsiI CCGC 1 cut(s) 506
SspMI CTAG 3 cut(s) 62, 426, 536
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 2 cut(s) 246, 463
StyI CCWWGG 1 cut(s) 397
TaqI TCGA 6 cut(s) 9, 224, 353, 362, 443, 544
TasI AATT 3 cut(s) 144, 310, 565
TatI WGTACW 3 cut(s) 18, 227, 369
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 6 cut(s) 198, 263, 455, 564, 678, 712
Tru9I TTAA 6 cut(s) 198, 263, 455, 564, 678, 712
TseI GCWGC 1 cut(s) 473
TspDTI ATGAA 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 1 cut(s) 144
XhoI CTCGAG 1 cut(s) 223
XmiI GTMKAC 1 cut(s) 66
XspI CTAG 3 cut(s) 62, 426, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.