Rroxscaffold_1G00066680

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001932
Physical Location & Seq
Forward (+)
88177406 .. 88178183
778 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00066680.1

Sequence Viewer

Length: 684 bp
ATGAATATTGAGGAGGAGTACATAGATGTGCTTTTGGTCCCTGCTGGGTTACTGATCATGCTAGTTTACCACCTCTTCCTCCTTTTCAAGTACTTCAATAAGCCTCTCTCCACATCCATAGGTTACGAAAACAACGACAAACGAATTTGGGTTGGAAAAATCTTGCAGTTGCAGGGTGATGATCTTAGAAAGAGTGTTAACACAGCTCTCAATGTCATGTCCTCGAGTACAACTGCAACTATTTATTTGGCAACAATCTCATTAACCCTCTGCTCACTTATTGGAGCTTGGATGGCAAACAACACCACCAATTTCTTCCCAAGAGAACTTATATACGGCAATTCAAGTGCGTCGATAGTTTCGATCAAGCACATATGCCTCTTAACCTGCTTTCTCCTTGGTTTTTCGTGCTTTGTTCAATCAGCTAGGAACTTCGTCCATTCGAACTTCCTATTGAGCACTCCGGGCGCGACGGCTAAATCTGATGTGAAGAAAGTGGAAAAAGCAGTTCAAAGAGGAAGTGAATTTTGGTTACTTGGGCTTAGAGCTCTATATTGTGCTCTTAATATTCTGCTGTGGTTTTTTGGACCTATACCGATGTTTGTATCCTCTATCATAATGGTGGTGATTCTGTATTGCCATGACTTTAGATCAGATGATCAAAAGGGTCTTAATGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

227

Amino Acids

25.49

Weight (kDa)

8.5

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 12 - 219 6.8e-56 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 395
AccII CGCG 1 cut(s) 470
AcsI RAATTY 2 cut(s) 144, 524
AfaI GTAC 3 cut(s) 20, 92, 229
AgsI TTSAA 5 cut(s) 88, 97, 345, 419, 512
AjuI GAANNNNNNNTTGG 2 cut(s) 511, 543
AluBI AGCT 4 cut(s) 206, 287, 425, 548
AluI AGCT 4 cut(s) 206, 287, 425, 548
Alw21I GWGCWC 3 cut(s) 461, 550, 562
Ama87I CYCGRG 1 cut(s) 223
ApoI RAATTY 2 cut(s) 144, 524
AspLEI GCGC 1 cut(s) 470
AspS9I GGNCC 2 cut(s) 37, 587
AsuC2I CCSGG 1 cut(s) 465
AsuHPI GGTGA 2 cut(s) 188, 637
AsuII TTCGAA 1 cut(s) 443
AvaI CYCGRG 1 cut(s) 223
AvaII GGWCC 2 cut(s) 37, 587
BanII GRGCYC 1 cut(s) 550
Bbv12I GWGCWC 3 cut(s) 461, 550, 562
BccI CCATC 1 cut(s) 286
BceAI ACGGC 2 cut(s) 352, 489
BciVI GTATCC 1 cut(s) 616
BclI TGATCA 2 cut(s) 54, 658
BcnI CCSGG 1 cut(s) 465
BfaI CTAG 2 cut(s) 62, 426
BfuAI ACCTGC 1 cut(s) 395
BfuI GTATCC 1 cut(s) 616
BmcAI AGTACT 1 cut(s) 92
Bme1390I CCNGG 1 cut(s) 465
Bme18I GGWCC 2 cut(s) 37, 587
BmeT110I CYCGRG 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 37, 587
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 465
Bpu14I TTCGAA 1 cut(s) 443
BpuMI CCSGG 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 397
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 2 cut(s) 113, 297
BseRI GAGGAG 2 cut(s) 26, 29
BseYI CCCAGC 1 cut(s) 44
Bsh1236I CGCG 1 cut(s) 470
BsiHKAI GWGCWC 3 cut(s) 461, 550, 562
BsiHKCI CYCGRG 1 cut(s) 223
BsiSI CCGG 1 cut(s) 464
BslFI GGGAC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 23
BsoBI CYCGRG 1 cut(s) 223
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 3 cut(s) 461, 550, 562
Bsp143I GATC 5 cut(s) 54, 181, 363, 650, 658
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 1 cut(s) 39
BspMI ACCTGC 1 cut(s) 395
BspT104I TTCGAA 1 cut(s) 443
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 5 cut(s) 54, 181, 363, 650, 658
BssT1I CCWWGG 1 cut(s) 397
Bst6I CTCTTC 1 cut(s) 80
BstBI TTCGAA 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 185, 542
BstF5I GGATG 2 cut(s) 113, 297
BstFNI CGCG 1 cut(s) 470
BstHHI GCGC 1 cut(s) 470
BstKTI GATC 5 cut(s) 57, 184, 366, 653, 661
BstMBI GATC 5 cut(s) 54, 181, 363, 650, 658
BstMWI GCNNNNNNNGC 2 cut(s) 293, 465
BstSCI CCNGG 1 cut(s) 463
BstUI CGCG 1 cut(s) 470
BsuI GTATCC 1 cut(s) 616
BtsCI GGATG 2 cut(s) 113, 297
BveI ACCTGC 1 cut(s) 395
CfoI GCGC 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 37, 587
CseI GACGC 1 cut(s) 339
Csp6I GTAC 3 cut(s) 19, 91, 228
CviAII CATG 3 cut(s) 58, 217, 641
CviJI RGCY 7 cut(s) 103, 206, 287, 425, 476, 541, 548
CviKI_1 RGCY 7 cut(s) 103, 206, 287, 425, 476, 541, 548
CviQI GTAC 3 cut(s) 19, 91, 228
DdeI CTNAG 2 cut(s) 185, 542
DpnI GATC 5 cut(s) 56, 183, 365, 652, 660
DpnII GATC 5 cut(s) 54, 181, 363, 650, 658
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Ecl136II GAGCTC 1 cut(s) 548
Eco130I CCWWGG 1 cut(s) 397
Eco24I GRGCYC 1 cut(s) 550
Eco47I GGWCC 2 cut(s) 37, 587
Eco53kI GAGCTC 1 cut(s) 548
Eco88I CYCGRG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 548
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 3 cut(s) 61, 220, 644
FaqI GGGAC 1 cut(s) 23
FatI CATG 3 cut(s) 57, 216, 640
FauNDI CATATG 1 cut(s) 374
FbaI TGATCA 2 cut(s) 54, 658
FokI GGATG 2 cut(s) 100, 304
FriOI GRGCYC 1 cut(s) 550
FspBI CTAG 2 cut(s) 62, 426
GlaI GCGC 1 cut(s) 469
GsaI CCCAGC 1 cut(s) 48
HapII CCGG 1 cut(s) 464
HgaI GACGC 1 cut(s) 339
HhaI GCGC 1 cut(s) 470
Hin1II CATG 3 cut(s) 61, 220, 644
Hin6I GCGC 1 cut(s) 468
HinP1I GCGC 1 cut(s) 468
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 1 cut(s) 628
HpaI GTTAAC 1 cut(s) 199
HpaII CCGG 1 cut(s) 464
HphI GGTGA 2 cut(s) 188, 637
Hpy166II GTNNAC 2 cut(s) 67, 199
Hpy188I TCNGA 2 cut(s) 484, 655
Hpy8I GTNNAC 2 cut(s) 67, 199
Hpy99I CGWCG 2 cut(s) 355, 475
HpyCH4V TGCA 3 cut(s) 166, 172, 236
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 465
HpyF3I CTNAG 2 cut(s) 185, 542
Hsp92II CATG 3 cut(s) 61, 220, 644
HspAI GCGC 1 cut(s) 468
Ksp22I TGATCA 2 cut(s) 54, 658
KspAI GTTAAC 1 cut(s) 199
Kzo9I GATC 5 cut(s) 54, 181, 363, 650, 658
LmnI GCTCC 1 cut(s) 284
LpnPI CCDG 5 cut(s) 30, 54, 158, 400, 477
MaeI CTAG 2 cut(s) 62, 426
MaeIII GTNAC 3 cut(s) 48, 122, 531
MalI GATC 5 cut(s) 56, 183, 365, 652, 660
MboI GATC 5 cut(s) 54, 181, 363, 650, 658
MboII GAAGA 3 cut(s) 67, 307, 502
MhlI GDGCHC 3 cut(s) 461, 550, 562
MluCI AATT 4 cut(s) 144, 310, 340, 524
MmeI TCCRAC 1 cut(s) 133
MseI TTAA 5 cut(s) 198, 263, 383, 564, 672
MslI CAYNNNNRTG 2 cut(s) 26, 620
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 2 cut(s) 293, 465
NciI CCSGG 1 cut(s) 465
NdeI CATATG 1 cut(s) 374
NdeII GATC 5 cut(s) 54, 181, 363, 650, 658
NlaIII CATG 3 cut(s) 61, 220, 644
NlaIV GGNNCC 1 cut(s) 39
NspV TTCGAA 1 cut(s) 443
PaeR7I CTCGAG 1 cut(s) 223
PcsI WCGNNNNNNNCGW 2 cut(s) 132, 359
PfeI GAWTC 1 cut(s) 628
Psp124BI GAGCTC 1 cut(s) 550
PspFI CCCAGC 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 587
PspXI VCTCGAGB 1 cut(s) 223
RsaI GTAC 3 cut(s) 20, 92, 229
RsaNI GTAC 3 cut(s) 19, 91, 228
RseI CAYNNNNRTG 2 cut(s) 26, 620
SacI GAGCTC 1 cut(s) 550
SaqAI TTAA 5 cut(s) 198, 263, 383, 564, 672
Sau3AI GATC 5 cut(s) 54, 181, 363, 650, 658
Sau96I GGNCC 2 cut(s) 37, 587
ScaI AGTACT 1 cut(s) 92
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 3 cut(s) 461, 550, 562
SetI ASST 8 cut(s) 75, 124, 208, 289, 389, 427, 550, 592
Sfr274I CTCGAG 1 cut(s) 223
SfuI TTCGAA 1 cut(s) 443
SinI GGWCC 2 cut(s) 37, 587
SlaI CTCGAG 1 cut(s) 223
SmiMI CAYNNNNRTG 2 cut(s) 26, 620
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 4 cut(s) 144, 310, 340, 524
SspI AATATT 2 cut(s) 7, 568
SspMI CTAG 2 cut(s) 62, 426
SstI GAGCTC 1 cut(s) 550
StyD4I CCNGG 1 cut(s) 463
StyI CCWWGG 1 cut(s) 397
TaqI TCGA 4 cut(s) 224, 353, 362, 443
TasI AATT 4 cut(s) 144, 310, 340, 524
TatI WGTACW 3 cut(s) 18, 90, 227
TfiI GAWTC 1 cut(s) 628
Tru1I TTAA 5 cut(s) 198, 263, 383, 564, 672
Tru9I TTAA 5 cut(s) 198, 263, 383, 564, 672
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 37, 587
XapI RAATTY 2 cut(s) 144, 524
XhoI CTCGAG 1 cut(s) 223
XspI CTAG 2 cut(s) 62, 426
ZrmI AGTACT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.