RchiOBHm_Chr5g0009441

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001932
Physical Location & Seq
Reverse (-)
6294280 .. 6294969
690 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29031

Sequence Viewer

Length: 567 bp
ATGAATATTGAGGAGTACATAGATGTGCTTTTGGTCCCTGCTGGGTTACTGATCATGCTAGTTTACCACCTCTTCCTCCTTTTCAAGTACTTCAATATGCCTCTCTCCACATCCATAGGTTACGAAAACAACGACAAACGAATTTGGGTTGGAAAAATCTTGCAGTTGCAGGGTGATGATCTTAGAAAGAGTGTTAAAACAGCTCTCAATGTGATTTCCTCGAGTACAACTGCAACTATTTATTTGGCAACAATCTCATTAACCCTCTGCTCACTTATCGGAGCTTGGATGGCAAACAACACCACCAATTTCTTCCCAAGAGAACTTATATACGGCAATACAAGTGCGTCGATAGTTTCGATCAAGTACATATGCCTCTTAACCTGTTTTCTCCTTGGTTTTTCGTGCTTTGTTCAATCAGCTAGGAACTTCGTCCATTCGAACTTCCTACTGAGCACTCCGGGCGCGACGGCTAAATCTGATGTGAAGAAAGTGGAAAAAGCAGTTCAAAGAGGAAGTGAATTTTGGTTACTTGGGCTTAGAGCTCTAGCTATATTGTGCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

188

Amino Acids

20.92

Weight (kDa)

8.99

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 183 3e-39 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 467
AcsI RAATTY 2 cut(s) 141, 521
AfaI GTAC 4 cut(s) 17, 89, 226, 368
AgsI TTSAA 4 cut(s) 85, 94, 416, 509
AjuI GAANNNNNNNTTGG 2 cut(s) 508, 540
AluBI AGCT 5 cut(s) 203, 284, 422, 545, 551
AluI AGCT 5 cut(s) 203, 284, 422, 545, 551
Alw21I GWGCWC 3 cut(s) 458, 547, 563
Ama87I CYCGRG 1 cut(s) 220
ApoI RAATTY 2 cut(s) 141, 521
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 220
AvaII GGWCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 547
Bbv12I GWGCWC 3 cut(s) 458, 547, 563
BccI CCATC 1 cut(s) 283
BceAI ACGGC 2 cut(s) 349, 486
BclI TGATCA 1 cut(s) 51
BcnI CCSGG 1 cut(s) 462
BfaI CTAG 3 cut(s) 59, 423, 548
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 1 cut(s) 34
BmeT110I CYCGRG 1 cut(s) 220
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 110, 294
BseMII CTCAG 1 cut(s) 443
BseRI GAGGAG 1 cut(s) 26
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 467
BsiHKAI GWGCWC 3 cut(s) 458, 547, 563
BsiHKCI CYCGRG 1 cut(s) 220
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 220
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 458, 547, 563
Bsp143I GATC 3 cut(s) 51, 178, 360
BspCNI CTCAG 1 cut(s) 444
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 440
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 3 cut(s) 51, 178, 360
BssT1I CCWWGG 1 cut(s) 394
Bst6I CTCTTC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 440
BstDEI CTNAG 3 cut(s) 182, 452, 539
BstF5I GGATG 2 cut(s) 110, 294
BstFNI CGCG 1 cut(s) 467
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 3 cut(s) 54, 181, 363
BstMBI GATC 3 cut(s) 51, 178, 360
BstMWI GCNNNNNNNGC 2 cut(s) 290, 462
BstSCI CCNGG 1 cut(s) 460
BstUI CGCG 1 cut(s) 467
BtsCI GGATG 2 cut(s) 110, 294
CfoI GCGC 1 cut(s) 467
Cfr13I GGNCC 1 cut(s) 34
CseI GACGC 1 cut(s) 336
Csp6I GTAC 4 cut(s) 16, 88, 225, 367
CviAII CATG 1 cut(s) 55
CviJI RGCY 7 cut(s) 203, 284, 422, 473, 538, 545, 551
CviKI_1 RGCY 7 cut(s) 203, 284, 422, 473, 538, 545, 551
CviQI GTAC 4 cut(s) 16, 88, 225, 367
DdeI CTNAG 3 cut(s) 182, 452, 539
DpnI GATC 3 cut(s) 53, 180, 362
DpnII GATC 3 cut(s) 51, 178, 360
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 547
Eco47I GGWCC 1 cut(s) 34
Eco53kI GAGCTC 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 545
EcoT14I CCWWGG 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 1 cut(s) 58
FaiI YATR 9 cut(s) 20, 56, 98, 116, 329, 331, 371, 373, 554
FaqI GGGAC 1 cut(s) 20
FatI CATG 1 cut(s) 54
FauNDI CATATG 1 cut(s) 371
FbaI TGATCA 1 cut(s) 51
FokI GGATG 2 cut(s) 97, 301
FriOI GRGCYC 1 cut(s) 547
FspBI CTAG 3 cut(s) 59, 423, 548
GlaI GCGC 1 cut(s) 466
GsaI CCCAGC 1 cut(s) 45
HapII CCGG 1 cut(s) 461
HgaI GACGC 1 cut(s) 336
HhaI GCGC 1 cut(s) 467
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HpaII CCGG 1 cut(s) 461
HphI GGTGA 1 cut(s) 185
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 2 cut(s) 281, 481
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 2 cut(s) 352, 472
HpyCH4V TGCA 3 cut(s) 163, 169, 233
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 462
HpyF3I CTNAG 3 cut(s) 182, 452, 539
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 1 cut(s) 465
Ksp22I TGATCA 1 cut(s) 51
Kzo9I GATC 3 cut(s) 51, 178, 360
LmnI GCTCC 1 cut(s) 281
LpnPI CCDG 5 cut(s) 27, 51, 155, 397, 474
MaeI CTAG 3 cut(s) 59, 423, 548
MaeIII GTNAC 3 cut(s) 45, 119, 528
MalI GATC 3 cut(s) 53, 180, 362
MboI GATC 3 cut(s) 51, 178, 360
MboII GAAGA 3 cut(s) 64, 304, 499
MhlI GDGCHC 3 cut(s) 458, 547, 563
MluCI AATT 3 cut(s) 141, 307, 521
MmeI TCCRAC 1 cut(s) 130
MnlI CCTC 8 cut(s) 4, 80, 86, 111, 229, 275, 386, 506
MseI TTAA 4 cut(s) 195, 260, 380, 565
MslI CAYNNNNRTG 1 cut(s) 23
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 462
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 2 cut(s) 290, 462
NciI CCSGG 1 cut(s) 462
NdeI CATATG 1 cut(s) 371
NdeII GATC 3 cut(s) 51, 178, 360
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 36
NspV TTCGAA 1 cut(s) 440
PaeR7I CTCGAG 1 cut(s) 220
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 356
Psp124BI GAGCTC 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 1 cut(s) 34
PspXI VCTCGAGB 1 cut(s) 220
RsaI GTAC 4 cut(s) 17, 89, 226, 368
RsaNI GTAC 4 cut(s) 16, 88, 225, 367
RseI CAYNNNNRTG 1 cut(s) 23
SacI GAGCTC 1 cut(s) 547
SaqAI TTAA 4 cut(s) 195, 260, 380, 565
Sau3AI GATC 3 cut(s) 51, 178, 360
Sau96I GGNCC 1 cut(s) 34
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 3 cut(s) 458, 547, 563
SetI ASST 8 cut(s) 72, 121, 205, 286, 386, 424, 547, 553
Sfr274I CTCGAG 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 440
SinI GGWCC 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 220
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 3 cut(s) 141, 307, 521
SspI AATATT 1 cut(s) 7
SspMI CTAG 3 cut(s) 59, 423, 548
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 1 cut(s) 394
TaqI TCGA 4 cut(s) 221, 350, 359, 440
TasI AATT 3 cut(s) 141, 307, 521
TatI WGTACW 4 cut(s) 15, 87, 224, 366
Tru1I TTAA 4 cut(s) 195, 260, 380, 565
Tru9I TTAA 4 cut(s) 195, 260, 380, 565
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 34
XapI RAATTY 2 cut(s) 141, 521
XhoI CTCGAG 1 cut(s) 220
XspI CTAG 3 cut(s) 59, 423, 548
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.