Rw5G006840

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001932
Physical Location & Seq
Reverse (-)
7076611 .. 7077462
852 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G006840.1

Sequence Viewer

Length: 684 bp
ATGAATTTTGAGAGGGGGTACATCGATGTGGTTCTGGTCCCATCTGGGCTTCTGATCATGTTGATTTACCACCTCTTCTTCCTCTACAAGTATTTCCATCAACCTCTATCCACATCCATGGGGTATGAAAACAACGACAAGCGAGTTTGGGTTGCAAAAATTCTTCAGGTGCAGGGTGACAATGTTAACAGAGGACTTGGTGTGATTTCTTCCAATACAAGTGCAGCCATGACCTTAGCATCAATCTGTTTGACTCTCTCTTCTCTAATCGGAGTTTGGATAGCAAATTCTCCCAGCAAGTCCTTCCCAATAGAAGTGTTCTATGGCAATACGACTCCATCGATGAATTCAATCAAGTATATCTGCTTGTTGACCTCTTTTCTGCTTGCTTTCTCGTGCTTCGTTCAATCGGCTAGGCACTTTGTCCATTCGAACTACCTATTGAGCACTCCGGGGGCTAGCGATAAAGGGGTTGTGAAGAAGGTGGAAAGAGCAGTTCAAAGAGGAAGTGAATTTTGGTCGCTTGGGCTTAGAGCACTGTATTTTGCTCTAAACTTTCTGCTGTGGTTTTTTGGACCTATACCTATGTTTGTTTCCTCTATCATAATGGTGGTGATTCTGTATTGCCATGATTTTAGGAAGTTGGAATCGGATAATCTCCATGATGTATTATTGCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

227

Amino Acids

25.6

Weight (kDa)

8.87

Isoelectric Point (pI)

28.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 213 3.8e-55 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 5 cut(s) 4, 159, 286, 346, 512
AcuI CTGAAG 1 cut(s) 149
AfaI GTAC 1 cut(s) 20
AgsI TTSAA 3 cut(s) 351, 407, 500
AjuI GAANNNNNNNTTGG 2 cut(s) 499, 531
Alw21I GWGCWC 2 cut(s) 449, 538
ApeKI GCWGC 1 cut(s) 224
ApoI RAATTY 5 cut(s) 4, 159, 286, 346, 512
AspS9I GGNCC 2 cut(s) 37, 575
AsuC2I CCSGG 1 cut(s) 453
AsuHPI GGTGA 2 cut(s) 188, 625
AsuII TTCGAA 1 cut(s) 431
AsuNHI GCTAGC 1 cut(s) 458
AvaII GGWCC 2 cut(s) 37, 575
BauI CACGAG 1 cut(s) 394
Bbv12I GWGCWC 2 cut(s) 449, 538
BbvI GCAGC 1 cut(s) 236
BccI CCATC 3 cut(s) 49, 105, 346
BclI TGATCA 1 cut(s) 54
BcnI CCSGG 1 cut(s) 453
BfaI CTAG 2 cut(s) 414, 459
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 2 cut(s) 37, 575
BmgT120I GGNCC 2 cut(s) 37, 575
BmiI GGNNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 1 cut(s) 248
BmtI GCTAGC 1 cut(s) 462
Bpu10I CCTNAGC 1 cut(s) 235
Bpu14I TTCGAA 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 453
Bsa29I ATCGAT 2 cut(s) 24, 341
BsaJI CCNNGG 2 cut(s) 117, 452
BseCI ATCGAT 2 cut(s) 24, 341
BseDI CCNNGG 2 cut(s) 117, 452
BseGI GGATG 1 cut(s) 113
BseXI GCAGC 1 cut(s) 236
BseYI CCCAGC 1 cut(s) 293
BsgI GTGCAG 2 cut(s) 191, 243
BshVI ATCGAT 2 cut(s) 24, 341
BsiHKAI GWGCWC 2 cut(s) 449, 538
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 2 cut(s) 449, 538
Bsp143I GATC 1 cut(s) 54
Bsp19I CCATGG 1 cut(s) 117
BspDI ATCGAT 2 cut(s) 24, 341
BspLI GGNNCC 1 cut(s) 39
BspOI GCTAGC 1 cut(s) 462
BspT104I TTCGAA 1 cut(s) 431
BssECI CCNNGG 2 cut(s) 117, 452
BssMI GATC 1 cut(s) 54
BssSI CACGAG 1 cut(s) 394
BssT1I CCWWGG 1 cut(s) 117
Bst2BI CACGAG 1 cut(s) 394
Bst4CI ACNGT 1 cut(s) 540
Bst6I CTCTTC 2 cut(s) 80, 265
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 2 cut(s) 387, 460
BstDEI CTNAG 2 cut(s) 235, 530
BstDSI CCRYGG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 113
BstKTI GATC 1 cut(s) 57
BstMBI GATC 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 451
BstV1I GCAGC 1 cut(s) 236
BstXI CCANNNNNNTGG 1 cut(s) 118
Bsu15I ATCGAT 2 cut(s) 24, 341
BsuTUI ATCGAT 2 cut(s) 24, 341
BtgI CCRYGG 1 cut(s) 117
BtsCI GGATG 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 2 cut(s) 387, 460
Cfr13I GGNCC 2 cut(s) 37, 575
ClaI ATCGAT 2 cut(s) 24, 341
Csp6I GTAC 1 cut(s) 19
CviAII CATG 5 cut(s) 58, 118, 229, 629, 662
CviJI RGCY 5 cut(s) 49, 227, 413, 458, 529
CviKI_1 RGCY 5 cut(s) 49, 227, 413, 458, 529
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 2 cut(s) 235, 530
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 80, 265
EarI CTCTTC 2 cut(s) 80, 265
Eco130I CCWWGG 1 cut(s) 117
Eco47I GGWCC 2 cut(s) 37, 575
Eco57I CTGAAG 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 346
EcoT14I CCWWGG 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 117
FaeI CATG 5 cut(s) 61, 121, 232, 632, 665
FaqI GGGAC 1 cut(s) 23
FatI CATG 5 cut(s) 57, 117, 228, 628, 661
FbaI TGATCA 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 2 cut(s) 414, 459
GluI GCNGC 1 cut(s) 225
GsaI CCCAGC 1 cut(s) 297
HapII CCGG 1 cut(s) 452
Hin1II CATG 5 cut(s) 61, 121, 232, 632, 665
HincII GTYRAC 2 cut(s) 187, 372
HindII GTYRAC 2 cut(s) 187, 372
HinfI GANTC 4 cut(s) 253, 334, 616, 647
HpaI GTTAAC 1 cut(s) 187
HpaII CCGG 1 cut(s) 452
HphI GGTGA 2 cut(s) 188, 625
Hpy166II GTNNAC 2 cut(s) 187, 372
Hpy188I TCNGA 3 cut(s) 54, 272, 652
Hpy188III TCNNGA 1 cut(s) 681
Hpy8I GTNNAC 2 cut(s) 187, 372
HpyAV CCTTC 2 cut(s) 313, 475
HpyCH4III ACNGT 1 cut(s) 540
HpyCH4V TGCA 3 cut(s) 155, 172, 224
HpyF3I CTNAG 2 cut(s) 235, 530
Hsp92II CATG 5 cut(s) 61, 121, 232, 632, 665
Ksp22I TGATCA 1 cut(s) 54
KspAI GTTAAC 1 cut(s) 187
Kzo9I GATC 1 cut(s) 54
LpnPI CCDG 6 cut(s) 20, 30, 152, 158, 307, 465
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 1 cut(s) 248
MaeI CTAG 2 cut(s) 414, 459
MaeIII GTNAC 1 cut(s) 176
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 6 cut(s) 67, 70, 155, 201, 252, 490
MhlI GDGCHC 2 cut(s) 449, 538
MluCI AATT 5 cut(s) 4, 159, 286, 346, 512
MlyI GAGTC 2 cut(s) 247, 328
MmeI TCCRAC 1 cut(s) 624
MnlI CCTC 8 cut(s) 6, 83, 92, 114, 185, 385, 497, 607
MseI TTAA 1 cut(s) 186
MslI CAYNNNNRTG 3 cut(s) 26, 116, 608
MspI CCGG 1 cut(s) 452
MspR9I CCNGG 1 cut(s) 453
NciI CCSGG 1 cut(s) 453
NcoI CCATGG 1 cut(s) 117
NdeII GATC 1 cut(s) 54
NheI GCTAGC 1 cut(s) 458
NlaIII CATG 5 cut(s) 61, 121, 232, 632, 665
NlaIV GGNNCC 1 cut(s) 39
NmuCI GTSAC 1 cut(s) 176
NspV TTCGAA 1 cut(s) 431
PcsI WCGNNNNNNNCGW 1 cut(s) 338
PfeI GAWTC 2 cut(s) 616, 647
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 2 cut(s) 247, 328
PpsI GAGTC 2 cut(s) 247, 328
PspFI CCCAGC 1 cut(s) 293
PspN4I GGNNCC 1 cut(s) 39
PspPI GGNCC 2 cut(s) 37, 575
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
RseI CAYNNNNRTG 3 cut(s) 26, 116, 608
SaqAI TTAA 1 cut(s) 186
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 37, 575
SchI GAGTC 2 cut(s) 247, 328
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 2 cut(s) 449, 538
SetI ASST 9 cut(s) 75, 106, 171, 236, 377, 441, 486, 580, 586
SfaNI GCATC 1 cut(s) 248
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 2 cut(s) 37, 575
SmiMI CAYNNNNRTG 3 cut(s) 26, 116, 608
Sse9I AATT 5 cut(s) 4, 159, 286, 346, 512
SspMI CTAG 2 cut(s) 414, 459
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 117
TaaI ACNGT 1 cut(s) 540
TaqI TCGA 3 cut(s) 24, 341, 431
TasI AATT 5 cut(s) 4, 159, 286, 346, 512
TfiI GAWTC 2 cut(s) 616, 647
Tru1I TTAA 1 cut(s) 186
Tru9I TTAA 1 cut(s) 186
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 176
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 3 cut(s) 17, 141, 359
TspRI CASTG 1 cut(s) 543
VpaK11BI GGWCC 2 cut(s) 37, 575
XapI RAATTY 5 cut(s) 4, 159, 286, 346, 512
XspI CTAG 2 cut(s) 414, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.