Rroxscaffold_1G00067380

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001932
Physical Location & Seq
Forward (+)
88689497 .. 88690223
727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067380.1

Sequence Viewer

Length: 645 bp
ATGTTTCTTTACCACCTCTTCCTCCTGTACCAATACATCCATGACGCCACCAAAACATCCATCGGTTACGAAAACAAAGACAAAGAAACTTGGGTTCAAAAGATTTTGAAGGTCACTGAGGGTACTAATGATGATGCAGTTGGGGGCAATGTTAGCACAGGACTTAGTGTGATTTCCTCAAACACAAGCGCAACCATGACCTTAGCGTCAATCTCCTTAACTCTCTGCGCTGTTATCGGAGTCTGGATTGCAAGTTCAACCAATAAGTTCTTCCCATTAGAAATGGTTTACGGCAACACAAGCGAGTCAATCATTTCTATCAAGTACTTATGCCTGTTGAGCTGCTTTCTCCTTGCTTTTTCGTTCTTCGTTCAATCTGCGAGGCACTTTGTCCATTCAAACTACCTATTGAGCACTCCAGGAGCCACGAAAGAAGAGGATGTGGAAAAAGTGCAGAAAGCGGTTGAAAGAGGAAGTATCTTTTGGTCATTGGGACTCAGAGCACTATATTTTGCTCTTAATTTTCTGCTGTGGTTCTTCGGGCCTGTACCAATGTGTGCTTGCTCTATTGTAATGGTATTTGTCCTGTACTGCCATGATTTCAGGAAGGACTCGAAACCGTCAGATAGTCGAAAGAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

214

Amino Acids

23.99

Weight (kDa)

8.17

Isoelectric Point (pI)

25.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 1 - 203 1e-53 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 205
AciI CCGC 1 cut(s) 461
AcyI GRCGYC 1 cut(s) 45
AfaI GTAC 5 cut(s) 29, 124, 326, 549, 590
AgsI TTSAA 6 cut(s) 98, 109, 258, 374, 399, 467
AjnI CCWGG 1 cut(s) 418
AjuI GAANNNNNNNTTGG 4 cut(s) 254, 286, 466, 498
AloI GAACNNNNNNTCC 2 cut(s) 238, 270
AluBI AGCT 1 cut(s) 342
AluI AGCT 1 cut(s) 342
Alw21I GWGCWC 2 cut(s) 416, 505
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 1 cut(s) 342
AspLEI GCGC 2 cut(s) 191, 230
AspS9I GGNCC 1 cut(s) 542
Bbv12I GWGCWC 2 cut(s) 416, 505
BbvI GCAGC 1 cut(s) 329
BccI CCATC 1 cut(s) 68
BceAI ACGGC 1 cut(s) 307
BciT130I CCWGG 1 cut(s) 420
BisI GCNGC 1 cut(s) 343
BlsI GCNGC 1 cut(s) 344
BmcAI AGTACT 1 cut(s) 326
Bme1390I CCNGG 1 cut(s) 420
BmgT120I GGNCC 1 cut(s) 542
BmiI GGNNCC 1 cut(s) 424
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 1 cut(s) 124
BpmI CTGGAG 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 202
BsaHI GRCGYC 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 420
BseGI GGATG 3 cut(s) 36, 56, 445
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 2 cut(s) 108, 511
BseXI GCAGC 1 cut(s) 329
BsgI GTGCAG 1 cut(s) 473
BshFI GGCC 1 cut(s) 544
BsiHKAI GWGCWC 2 cut(s) 416, 505
BslFI GGGAC 1 cut(s) 507
BsmFI GGGAC 1 cut(s) 507
BsnI GGCC 1 cut(s) 544
Bsp1286I GDGCHC 2 cut(s) 416, 505
BspACI CCGC 1 cut(s) 461
BspANI GGCC 1 cut(s) 544
BspCNI CTCAG 2 cut(s) 109, 510
BspLI GGNNCC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 154
BssNI GRCGYC 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 1 cut(s) 621
Bst6I CTCTTC 2 cut(s) 23, 429
BstACI GRCGYC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 562
BstDEI CTNAG 4 cut(s) 117, 164, 202, 497
BstF5I GGATG 3 cut(s) 36, 56, 445
BstHHI GCGC 2 cut(s) 191, 230
BstMWI GCNNNNNNNGC 3 cut(s) 153, 300, 339
BstNI CCWGG 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 418
BstV1I GCAGC 1 cut(s) 329
BsuRI GGCC 1 cut(s) 544
BtsCI GGATG 3 cut(s) 36, 56, 445
BtsIMutI CAGTG 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 562
CfoI GCGC 2 cut(s) 191, 230
Cfr13I GGNCC 1 cut(s) 542
CseI GACGC 2 cut(s) 53, 195
Csp6I GTAC 5 cut(s) 28, 123, 325, 548, 589
CviAII CATG 3 cut(s) 41, 196, 596
CviJI RGCY 3 cut(s) 342, 425, 544
CviKI_1 RGCY 3 cut(s) 342, 425, 544
CviQI GTAC 5 cut(s) 28, 123, 325, 548, 589
DdeI CTNAG 4 cut(s) 117, 164, 202, 497
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 23, 429
EarI CTCTTC 2 cut(s) 23, 429
EcoRII CCWGG 1 cut(s) 418
FaeI CATG 3 cut(s) 44, 199, 599
FaiI YATR 5 cut(s) 42, 197, 331, 508, 597
FaqI GGGAC 1 cut(s) 507
FatI CATG 3 cut(s) 40, 195, 595
Fnu4HI GCNGC 1 cut(s) 343
FokI GGATG 3 cut(s) 23, 43, 452
Fsp4HI GCNGC 1 cut(s) 343
GlaI GCGC 2 cut(s) 190, 229
GluI GCNGC 1 cut(s) 343
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 1 cut(s) 544
HgaI GACGC 2 cut(s) 53, 195
HhaI GCGC 2 cut(s) 191, 230
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 3 cut(s) 44, 199, 599
Hin6I GCGC 2 cut(s) 189, 228
HinP1I GCGC 2 cut(s) 189, 228
HinfI GANTC 4 cut(s) 240, 305, 495, 611
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 3 cut(s) 239, 500, 625
Hpy188III TCNNGA 2 cut(s) 244, 604
Hpy8I GTNNAC 1 cut(s) 289
HpyAV CCTTC 3 cut(s) 103, 601, 631
HpyCH4III ACNGT 1 cut(s) 621
HpyCH4V TGCA 3 cut(s) 137, 251, 454
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 300, 339
HpyF3I CTNAG 4 cut(s) 117, 164, 202, 497
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 3 cut(s) 44, 199, 599
HspAI GCGC 2 cut(s) 189, 228
LmnI GCTCC 1 cut(s) 422
LpnPI CCDG 9 cut(s) 38, 144, 229, 347, 405, 432, 558, 589, 599
Lsp1109I GCAGC 1 cut(s) 329
LweI GCATC 1 cut(s) 124
MaeIII GTNAC 2 cut(s) 65, 112
MboII GAAGA 5 cut(s) 10, 262, 358, 446, 529
MhlI GDGCHC 2 cut(s) 416, 505
MluCI AATT 1 cut(s) 520
MlyI GAGTC 4 cut(s) 249, 314, 489, 605
MnlI CCTC 7 cut(s) 26, 32, 112, 187, 375, 430, 464
MseI TTAA 2 cut(s) 218, 519
MspR9I CCNGG 1 cut(s) 420
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 3 cut(s) 153, 300, 339
NlaIII CATG 3 cut(s) 44, 199, 599
NlaIV GGNNCC 1 cut(s) 424
NmuCI GTSAC 1 cut(s) 112
PfoI TCCNGGA 1 cut(s) 418
PkrI GCNGC 1 cut(s) 344
PleI GAGTC 4 cut(s) 248, 313, 489, 605
PpsI GAGTC 4 cut(s) 248, 313, 489, 605
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 424
PspPI GGNCC 1 cut(s) 542
RsaI GTAC 5 cut(s) 29, 124, 326, 549, 590
RsaNI GTAC 5 cut(s) 28, 123, 325, 548, 589
SaqAI TTAA 2 cut(s) 218, 519
SatI GCNGC 1 cut(s) 343
Sau96I GGNCC 1 cut(s) 542
ScaI AGTACT 1 cut(s) 326
SchI GAGTC 4 cut(s) 249, 314, 489, 605
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 2 cut(s) 416, 505
SetI ASST 5 cut(s) 18, 114, 203, 344, 408
SfaNI GCATC 1 cut(s) 124
Sse9I AATT 1 cut(s) 520
SsiI CCGC 1 cut(s) 461
StyD4I CCNGG 1 cut(s) 418
TaaI ACNGT 1 cut(s) 621
TaqI TCGA 2 cut(s) 614, 631
TasI AATT 1 cut(s) 520
TatI WGTACW 2 cut(s) 324, 588
Tru1I TTAA 2 cut(s) 218, 519
Tru9I TTAA 2 cut(s) 218, 519
TscAI CASTG 1 cut(s) 121
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 1 cut(s) 342
Tsp45I GTSAC 1 cut(s) 112
TspRI CASTG 1 cut(s) 121
ZrmI AGTACT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.