Rh5AG074100

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001932
Physical Location & Seq
Reverse (-)
6298163 .. 6298937
775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG074100.1

Sequence Viewer

Length: 681 bp
ATGAATATTGAGGAGTACATAGATGTGCTTTTGGTCCCTGCTGGGTTACTGATCATGCTAGTTTACCACCTCTTCCTCCTTTTCAAGTACATCAATAAGCCTCTCTCCACATCCATAGGTTACGAAAACAACGACAAACGAATTTGGGTTGGAAAAATCTTGCAGTTGCAGGGTGATGATCTTAGAAAGAGTGTTAAAACAGCTCTCAATGTGATTTCCTCGAGTACAACTGCAACTATTTATTTGGCAACAATCTCATTAACCCTCTGCTCACTTATCGGAGCTTGGATGGCAAACAACACCACCAATTTCCTCCCAAGAGAACTTATATACGGCAACACAAGTGCGTCGATAGTTTCGATCAAGTACATATGCCTCTTAACCTGTTTTCTCCTTGGTTTTTCGTGCTTTGTTCAATCAGCTAGGAACTTCGTCCATTCGAACTTCCTATTGAGCACTCCGGGCGCGACGGCTAAATCTGATGTGAAGAAAGTGGAAAAAGCAGTTCAAAGAGGAAGTGAATTTTGGTTACTTGGGCTTAGAGCTCTATATTGTGCTCTTAATTTTCTGCTGTGGTTTTTTGGACCTATACCGATGTTTGTATCCTCTATCATAATGGTGGTGATTCTGTATTGCCATGACTTTAGATCAGATGATCAAAAGGGTCTTAATGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

226

Amino Acids

25.36

Weight (kDa)

8.87

Isoelectric Point (pI)

29.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 11 - 218 3.9e-57 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 467
AcsI RAATTY 2 cut(s) 141, 521
AfaI GTAC 4 cut(s) 17, 89, 226, 368
AgsI TTSAA 3 cut(s) 85, 416, 509
AjuI GAANNNNNNNTTGG 2 cut(s) 508, 540
AluBI AGCT 4 cut(s) 203, 284, 422, 545
AluI AGCT 4 cut(s) 203, 284, 422, 545
Alw21I GWGCWC 3 cut(s) 458, 547, 559
Ama87I CYCGRG 1 cut(s) 220
ApoI RAATTY 2 cut(s) 141, 521
AspLEI GCGC 1 cut(s) 467
AspS9I GGNCC 2 cut(s) 34, 584
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 2 cut(s) 185, 634
AsuII TTCGAA 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 220
AvaII GGWCC 2 cut(s) 34, 584
BanII GRGCYC 1 cut(s) 547
Bbv12I GWGCWC 3 cut(s) 458, 547, 559
BccI CCATC 1 cut(s) 283
BceAI ACGGC 2 cut(s) 349, 486
BciVI GTATCC 1 cut(s) 613
BclI TGATCA 2 cut(s) 51, 655
BcnI CCSGG 1 cut(s) 462
BfaI CTAG 2 cut(s) 59, 423
BfuI GTATCC 1 cut(s) 613
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 2 cut(s) 34, 584
BmeT110I CYCGRG 1 cut(s) 220
BmgT120I GGNCC 2 cut(s) 34, 584
BmiI GGNNCC 1 cut(s) 36
BmrFI CCNGG 1 cut(s) 462
Bpu14I TTCGAA 1 cut(s) 440
BpuMI CCSGG 1 cut(s) 462
BsaJI CCNNGG 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 394
BseGI GGATG 2 cut(s) 110, 294
BseRI GAGGAG 1 cut(s) 26
BseYI CCCAGC 1 cut(s) 41
Bsh1236I CGCG 1 cut(s) 467
BsiHKAI GWGCWC 3 cut(s) 458, 547, 559
BsiHKCI CYCGRG 1 cut(s) 220
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 20
BsoBI CYCGRG 1 cut(s) 220
Bsp119I TTCGAA 1 cut(s) 440
Bsp1286I GDGCHC 3 cut(s) 458, 547, 559
Bsp143I GATC 5 cut(s) 51, 178, 360, 647, 655
BspFNI CGCG 1 cut(s) 467
BspLI GGNNCC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 440
BssECI CCNNGG 1 cut(s) 394
BssMI GATC 5 cut(s) 51, 178, 360, 647, 655
BssT1I CCWWGG 1 cut(s) 394
Bst6I CTCTTC 1 cut(s) 77
BstBI TTCGAA 1 cut(s) 440
BstDEI CTNAG 2 cut(s) 182, 539
BstF5I GGATG 2 cut(s) 110, 294
BstFNI CGCG 1 cut(s) 467
BstHHI GCGC 1 cut(s) 467
BstKTI GATC 5 cut(s) 54, 181, 363, 650, 658
BstMBI GATC 5 cut(s) 51, 178, 360, 647, 655
BstMWI GCNNNNNNNGC 2 cut(s) 290, 462
BstSCI CCNGG 1 cut(s) 460
BstUI CGCG 1 cut(s) 467
BsuI GTATCC 1 cut(s) 613
BtsCI GGATG 2 cut(s) 110, 294
CfoI GCGC 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 34, 584
CseI GACGC 1 cut(s) 336
Csp6I GTAC 4 cut(s) 16, 88, 225, 367
CviAII CATG 2 cut(s) 55, 638
CviJI RGCY 7 cut(s) 100, 203, 284, 422, 473, 538, 545
CviKI_1 RGCY 7 cut(s) 100, 203, 284, 422, 473, 538, 545
CviQI GTAC 4 cut(s) 16, 88, 225, 367
DdeI CTNAG 2 cut(s) 182, 539
DpnI GATC 5 cut(s) 53, 180, 362, 649, 657
DpnII GATC 5 cut(s) 51, 178, 360, 647, 655
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
Ecl136II GAGCTC 1 cut(s) 545
Eco130I CCWWGG 1 cut(s) 394
Eco24I GRGCYC 1 cut(s) 547
Eco47I GGWCC 2 cut(s) 34, 584
Eco53kI GAGCTC 1 cut(s) 545
Eco88I CYCGRG 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 545
EcoT14I CCWWGG 1 cut(s) 394
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 394
FaeI CATG 2 cut(s) 58, 641
FaqI GGGAC 1 cut(s) 20
FatI CATG 2 cut(s) 54, 637
FauNDI CATATG 1 cut(s) 371
FbaI TGATCA 2 cut(s) 51, 655
FokI GGATG 2 cut(s) 97, 301
FriOI GRGCYC 1 cut(s) 547
FspBI CTAG 2 cut(s) 59, 423
GlaI GCGC 1 cut(s) 466
GsaI CCCAGC 1 cut(s) 45
HapII CCGG 1 cut(s) 461
HgaI GACGC 1 cut(s) 336
HhaI GCGC 1 cut(s) 467
Hin1II CATG 2 cut(s) 58, 641
Hin6I GCGC 1 cut(s) 465
HinP1I GCGC 1 cut(s) 465
HinfI GANTC 1 cut(s) 625
HpaII CCGG 1 cut(s) 461
HphI GGTGA 2 cut(s) 185, 634
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 3 cut(s) 281, 481, 652
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 2 cut(s) 352, 472
HpyCH4V TGCA 3 cut(s) 163, 169, 233
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 462
HpyF3I CTNAG 2 cut(s) 182, 539
Hsp92II CATG 2 cut(s) 58, 641
HspAI GCGC 1 cut(s) 465
Ksp22I TGATCA 2 cut(s) 51, 655
Kzo9I GATC 5 cut(s) 51, 178, 360, 647, 655
LmnI GCTCC 1 cut(s) 281
LpnPI CCDG 5 cut(s) 27, 51, 155, 397, 474
MaeI CTAG 2 cut(s) 59, 423
MaeIII GTNAC 3 cut(s) 45, 119, 528
MalI GATC 5 cut(s) 53, 180, 362, 649, 657
MboI GATC 5 cut(s) 51, 178, 360, 647, 655
MboII GAAGA 2 cut(s) 64, 499
MhlI GDGCHC 3 cut(s) 458, 547, 559
MluCI AATT 4 cut(s) 141, 307, 521, 562
MmeI TCCRAC 1 cut(s) 130
MseI TTAA 5 cut(s) 195, 260, 380, 561, 669
MslI CAYNNNNRTG 2 cut(s) 23, 617
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 462
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 2 cut(s) 290, 462
NciI CCSGG 1 cut(s) 462
NdeI CATATG 1 cut(s) 371
NdeII GATC 5 cut(s) 51, 178, 360, 647, 655
NlaIII CATG 2 cut(s) 58, 641
NlaIV GGNNCC 1 cut(s) 36
NspV TTCGAA 1 cut(s) 440
PaeR7I CTCGAG 1 cut(s) 220
PcsI WCGNNNNNNNCGW 2 cut(s) 129, 356
PfeI GAWTC 1 cut(s) 625
Psp124BI GAGCTC 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 36
PspPI GGNCC 2 cut(s) 34, 584
PspXI VCTCGAGB 1 cut(s) 220
RsaI GTAC 4 cut(s) 17, 89, 226, 368
RsaNI GTAC 4 cut(s) 16, 88, 225, 367
RseI CAYNNNNRTG 2 cut(s) 23, 617
SacI GAGCTC 1 cut(s) 547
SaqAI TTAA 5 cut(s) 195, 260, 380, 561, 669
Sau3AI GATC 5 cut(s) 51, 178, 360, 647, 655
Sau96I GGNCC 2 cut(s) 34, 584
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 3 cut(s) 458, 547, 559
SetI ASST 8 cut(s) 72, 121, 205, 286, 386, 424, 547, 589
Sfr274I CTCGAG 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 440
SinI GGWCC 2 cut(s) 34, 584
SlaI CTCGAG 1 cut(s) 220
SmiMI CAYNNNNRTG 2 cut(s) 23, 617
SmlI CTYRAG 1 cut(s) 220
SmoI CTYRAG 1 cut(s) 220
Sse9I AATT 4 cut(s) 141, 307, 521, 562
SspI AATATT 1 cut(s) 7
SspMI CTAG 2 cut(s) 59, 423
SstI GAGCTC 1 cut(s) 547
StyD4I CCNGG 1 cut(s) 460
StyI CCWWGG 1 cut(s) 394
TaqI TCGA 4 cut(s) 221, 350, 359, 440
TasI AATT 4 cut(s) 141, 307, 521, 562
TatI WGTACW 4 cut(s) 15, 87, 224, 366
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 5 cut(s) 195, 260, 380, 561, 669
Tru9I TTAA 5 cut(s) 195, 260, 380, 561, 669
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 2 cut(s) 34, 584
XapI RAATTY 2 cut(s) 141, 521
XhoI CTCGAG 1 cut(s) 220
XspI CTAG 2 cut(s) 59, 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.