RchiOBHm_Chr5g0009431

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001932
Physical Location & Seq
Reverse (-)
6292637 .. 6294278
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29030

Sequence Viewer

Length: 786 bp
ATGTTTGTATCCTCTATCATAATGGTGGTGATTCTGTATTGCCATGACTTTAGATCAGATGATCAAAAGGGTCTTAATGAGACTATGGATTTTGAGAAAGAGTACTTGGATGCTGTTTTGGTCACAGCTGGGCTTCTGTTCATGTTTCTTTACCACCTCTTCCTCCTGTACCAATACATCCATGATGCCACCAAAACATCCATCGGTTACGAAAACAAAGACAAAGAAACTTGGGTTCAAAAGATTTTGAAGGTCACTGAGGGTACTAATGATGATGCAGTTGGGGACAATGTTAGCACAGGACTTAGTGTGATTTCCTCAAACACAAGCGCAACCATGACTTTAGCGTCAATCTCCTTAACTCTCTGCGCTGTTATCGGAGTCTGGATTGCAAGTTCAACCAATAAGTTCTTCCCATTAGAAATGGTTTACGGCAACACAAGCGAGTCAATCATTTCTATCAAGTACTTATGCCTGTTGAGCTGCTTTCTCCTTGCTTTTTCGTTCTTCGTTCAATCTGCGAGGCACTTTGTCCATTCAAACTACCTATTGAGCACTCCAGGAGCCACGAAAGAAGAGGATGTGGAAAAAGTGCAGAAAGCTGTTGAAAGAGGAAGTATCTTTTGGTCATTGGGACTCAGAGCACTATATTTTGCTCTTAATTTTCTGCTGTGGTTCTTCGGGCCTATACCAATGTGTGCTTGCTCTATTGTAATGGTATTTGTCCTGTACTGCCATGATTTCAGGAAGGACTCGAAACAGTCAGATAGTCGAAAGAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

261

Amino Acids

29.49

Weight (kDa)

5.86

Isoelectric Point (pI)

25.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 39 - 250 5.2e-56 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 346
AfaI GTAC 5 cut(s) 104, 170, 265, 467, 731
AgsI TTSAA 6 cut(s) 239, 250, 399, 515, 540, 608
AjnI CCWGG 1 cut(s) 559
AjuI GAANNNNNNNTTGG 6 cut(s) 89, 121, 395, 427, 607, 639
AloI GAACNNNNNNTCC 2 cut(s) 379, 411
AluBI AGCT 3 cut(s) 128, 483, 602
AluI AGCT 3 cut(s) 128, 483, 602
Alw21I GWGCWC 2 cut(s) 557, 646
Alw26I GTCTC 1 cut(s) 74
AoxI GGCC 1 cut(s) 683
ApeKI GCWGC 1 cut(s) 483
AspLEI GCGC 2 cut(s) 332, 371
AspS9I GGNCC 1 cut(s) 683
AsuHPI GGTGA 1 cut(s) 40
Bbv12I GWGCWC 2 cut(s) 557, 646
BbvI GCAGC 1 cut(s) 470
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 448
BciT130I CCWGG 1 cut(s) 561
BciVI GTATCC 1 cut(s) 19
BclI TGATCA 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 74
BfuI GTATCC 1 cut(s) 19
BisI GCNGC 1 cut(s) 484
BlsI GCNGC 1 cut(s) 485
BmcAI AGTACT 2 cut(s) 104, 467
Bme1390I CCNGG 1 cut(s) 561
BmgT120I GGNCC 1 cut(s) 683
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 1 cut(s) 561
BmsI GCATC 3 cut(s) 100, 175, 265
BpmI CTGGAG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 561
BseGI GGATG 4 cut(s) 115, 177, 197, 586
BseMII CTCAG 2 cut(s) 249, 652
BseXI GCAGC 1 cut(s) 470
BseYI CCCAGC 1 cut(s) 128
BsgI GTGCAG 1 cut(s) 614
BshFI GGCC 1 cut(s) 685
BsiHKAI GWGCWC 2 cut(s) 557, 646
BslFI GGGAC 2 cut(s) 299, 648
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 2 cut(s) 299, 648
BsnI GGCC 1 cut(s) 685
Bsp1286I GDGCHC 2 cut(s) 557, 646
Bsp143I GATC 2 cut(s) 53, 61
BspANI GGCC 1 cut(s) 685
BspCNI CTCAG 2 cut(s) 250, 651
BspLI GGNNCC 1 cut(s) 565
BssMI GATC 2 cut(s) 53, 61
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 1 cut(s) 762
Bst6I CTCTTC 2 cut(s) 164, 570
BstC8I GCNNGC 1 cut(s) 703
BstDEI CTNAG 3 cut(s) 258, 305, 638
BstF5I GGATG 4 cut(s) 115, 177, 197, 586
BstHHI GCGC 2 cut(s) 332, 371
BstKTI GATC 2 cut(s) 56, 64
BstMAI GTCTC 1 cut(s) 74
BstMBI GATC 2 cut(s) 53, 61
BstMWI GCNNNNNNNGC 2 cut(s) 441, 480
BstNI CCWGG 1 cut(s) 561
BstSCI CCNGG 1 cut(s) 559
BstV1I GCAGC 1 cut(s) 470
BsuI GTATCC 1 cut(s) 19
BsuRI GGCC 1 cut(s) 685
BtsCI GGATG 4 cut(s) 115, 177, 197, 586
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 703
CfoI GCGC 2 cut(s) 332, 371
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 1 cut(s) 336
Csp6I GTAC 5 cut(s) 103, 169, 264, 466, 730
CviAII CATG 5 cut(s) 44, 142, 182, 337, 737
CviJI RGCY 6 cut(s) 128, 133, 483, 566, 602, 685
CviKI_1 RGCY 6 cut(s) 128, 133, 483, 566, 602, 685
CviQI GTAC 5 cut(s) 103, 169, 264, 466, 730
DdeI CTNAG 3 cut(s) 258, 305, 638
DpnI GATC 2 cut(s) 55, 63
DpnII GATC 2 cut(s) 53, 61
DrdI GACNNNNNNGTC 1 cut(s) 346
DseDI GACNNNNNNGTC 1 cut(s) 346
Eam1104I CTCTTC 2 cut(s) 164, 570
EarI CTCTTC 2 cut(s) 164, 570
EcoRII CCWGG 1 cut(s) 559
FaeI CATG 5 cut(s) 47, 145, 185, 340, 740
FaqI GGGAC 2 cut(s) 299, 648
FatI CATG 5 cut(s) 43, 141, 181, 336, 736
FbaI TGATCA 1 cut(s) 61
Fnu4HI GCNGC 1 cut(s) 484
FokI GGATG 4 cut(s) 122, 164, 184, 593
Fsp4HI GCNGC 1 cut(s) 484
GlaI GCGC 2 cut(s) 331, 370
GluI GCNGC 1 cut(s) 484
GsaI CCCAGC 1 cut(s) 132
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 1 cut(s) 685
HgaI GACGC 1 cut(s) 336
HhaI GCGC 2 cut(s) 332, 371
Hin1II CATG 5 cut(s) 47, 145, 185, 340, 740
Hin6I GCGC 2 cut(s) 330, 369
HinP1I GCGC 2 cut(s) 330, 369
HinfI GANTC 5 cut(s) 31, 381, 446, 636, 752
HphI GGTGA 1 cut(s) 40
Hpy166II GTNNAC 1 cut(s) 430
Hpy188I TCNGA 4 cut(s) 58, 380, 641, 766
Hpy188III TCNNGA 2 cut(s) 385, 745
Hpy8I GTNNAC 1 cut(s) 430
HpyAV CCTTC 3 cut(s) 244, 742, 772
HpyCH4III ACNGT 1 cut(s) 762
HpyCH4V TGCA 3 cut(s) 278, 392, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 441, 480
HpyF3I CTNAG 3 cut(s) 258, 305, 638
Hsp92II CATG 5 cut(s) 47, 145, 185, 340, 740
HspAI GCGC 2 cut(s) 330, 369
Ksp22I TGATCA 1 cut(s) 61
Kzo9I GATC 2 cut(s) 53, 61
LmnI GCTCC 1 cut(s) 563
LpnPI CCDG 9 cut(s) 114, 179, 285, 370, 488, 546, 573, 730, 740
Lsp1109I GCAGC 1 cut(s) 470
LweI GCATC 3 cut(s) 100, 175, 265
MaeIII GTNAC 3 cut(s) 121, 206, 253
MalI GATC 2 cut(s) 55, 63
MboI GATC 2 cut(s) 53, 61
MboII GAAGA 5 cut(s) 151, 403, 499, 587, 670
MhlI GDGCHC 2 cut(s) 557, 646
MluCI AATT 1 cut(s) 661
MlyI GAGTC 4 cut(s) 390, 455, 630, 746
MnlI CCTC 8 cut(s) 22, 167, 173, 253, 328, 516, 571, 605
MseI TTAA 3 cut(s) 75, 359, 660
MslI CAYNNNNRTG 1 cut(s) 23
MspA1I CMGCKG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 561
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 2 cut(s) 441, 480
NdeII GATC 2 cut(s) 53, 61
NlaIII CATG 5 cut(s) 47, 145, 185, 340, 740
NlaIV GGNNCC 1 cut(s) 565
NmuCI GTSAC 2 cut(s) 121, 253
PfeI GAWTC 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 559
PkrI GCNGC 1 cut(s) 485
PleI GAGTC 4 cut(s) 389, 454, 630, 746
PpsI GAGTC 4 cut(s) 389, 454, 630, 746
Psp6I CCWGG 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 128
PspGI CCWGG 1 cut(s) 559
PspN4I GGNNCC 1 cut(s) 565
PspPI GGNCC 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 5 cut(s) 104, 170, 265, 467, 731
RsaNI GTAC 5 cut(s) 103, 169, 264, 466, 730
RseI CAYNNNNRTG 1 cut(s) 23
SaqAI TTAA 3 cut(s) 75, 359, 660
SatI GCNGC 1 cut(s) 484
Sau3AI GATC 2 cut(s) 53, 61
Sau96I GGNCC 1 cut(s) 683
ScaI AGTACT 2 cut(s) 104, 467
SchI GAGTC 4 cut(s) 390, 455, 630, 746
ScrFI CCNGG 1 cut(s) 561
SduI GDGCHC 2 cut(s) 557, 646
SetI ASST 6 cut(s) 130, 159, 255, 485, 549, 604
SfaNI GCATC 3 cut(s) 100, 175, 265
SmiMI CAYNNNNRTG 1 cut(s) 23
Sse9I AATT 1 cut(s) 661
StyD4I CCNGG 1 cut(s) 559
TaaI ACNGT 1 cut(s) 762
TaqI TCGA 2 cut(s) 755, 772
TasI AATT 1 cut(s) 661
TatI WGTACW 3 cut(s) 102, 465, 729
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 3 cut(s) 75, 359, 660
Tru9I TTAA 3 cut(s) 75, 359, 660
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 2 cut(s) 121, 253
TseI GCWGC 1 cut(s) 483
Tsp45I GTSAC 2 cut(s) 121, 253
TspDTI ATGAA 1 cut(s) 130
TspRI CASTG 1 cut(s) 262
ZrmI AGTACT 2 cut(s) 104, 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.