MD05G1309000.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001932
Physical Location & Seq
Reverse (-)
43988503 .. 43988877
375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1309000.v1.1.491

Sequence Viewer

Length: 375 bp
ATGGCAAACTCCATTAACAACTTCTTACCAAGTGAAATAATATATGGCAGCACAAACCCATTAACCGTTTCTATCAAGTACATCTGCATCTTGACATGTTTTCTCCTTGCTTTTTCATGCTTTGTTCAATCAGCTAGGCACTTTGTTCACTCAAACTGCCTTTTAGCCACCCCGGGAGCTACCGCGAAAAGCGACGTGAAAAAAGCCGATAGAGCAGTTGAAAGGGGAAGCGAGTTTTGGTCACTTGGGCTTAGAGCACTCTACTTTGCTCTCAATTTTCTGTTGTGGTTTTTTGGACCTATTCCCATGTTAGCTTTGTCTGTCGCGACGGTCGTGATCCTATCTTGCCACGACTTTGAGAAAGTCTGGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

125

Amino Acids

13.85

Weight (kDa)

8.69

Isoelectric Point (pI)

28.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 5 - 120 1.7e-35 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 185, 326
AciI CCGC 2 cut(s) 183, 370
AclWI GGATC 1 cut(s) 331
AfaI GTAC 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 95
AgsI TTSAA 2 cut(s) 128, 221
AjiI CACGTC 1 cut(s) 196
AjuI GAANNNNNNNTTGG 2 cut(s) 220, 252
AluBI AGCT 3 cut(s) 134, 179, 314
AluI AGCT 3 cut(s) 134, 179, 314
Alw21I GWGCWC 1 cut(s) 259
AlwI GGATC 1 cut(s) 331
Ama87I CYCGRG 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 296
AsuC2I CCSGG 2 cut(s) 173, 174
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 1 cut(s) 60
BcnI CCSGG 2 cut(s) 173, 174
BfaI CTAG 1 cut(s) 135
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
Bme1390I CCNGG 2 cut(s) 173, 174
Bme18I GGWCC 1 cut(s) 296
BmeT110I CYCGRG 1 cut(s) 172
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 1 cut(s) 296
BmrFI CCNGG 2 cut(s) 173, 174
BmsI GCATC 1 cut(s) 96
BpuMI CCSGG 2 cut(s) 173, 174
BsaJI CCNNGG 2 cut(s) 171, 172
BseDI CCNNGG 2 cut(s) 171, 172
BseXI GCAGC 1 cut(s) 60
Bsh1236I CGCG 2 cut(s) 185, 326
Bsh1285I CGRYCG 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 333
BsiHKAI GWGCWC 1 cut(s) 259
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 1 cut(s) 173
BsoBI CYCGRG 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 1 cut(s) 336
Bsp68I TCGCGA 1 cut(s) 326
BspACI CCGC 2 cut(s) 183, 370
BspFNI CGCG 2 cut(s) 185, 326
BspPI GGATC 1 cut(s) 331
BssECI CCNNGG 2 cut(s) 171, 172
BssMI GATC 1 cut(s) 336
Bst4CI ACNGT 2 cut(s) 67, 331
BstDEI CTNAG 1 cut(s) 251
BstFNI CGCG 2 cut(s) 185, 326
BstKTI GATC 1 cut(s) 339
BstMBI GATC 1 cut(s) 336
BstMCI CGRYCG 1 cut(s) 333
BstMWI GCNNNNNNNGC 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 171, 172
BstUI CGCG 2 cut(s) 185, 326
BstV1I GCAGC 1 cut(s) 60
BtrI CACGTC 1 cut(s) 196
BtuMI TCGCGA 1 cut(s) 326
Cfr13I GGNCC 1 cut(s) 296
Cfr9I CCCGGG 1 cut(s) 172
Csp6I GTAC 1 cut(s) 79
CviAII CATG 3 cut(s) 96, 117, 307
CviJI RGCY 6 cut(s) 134, 167, 179, 206, 250, 314
CviKI_1 RGCY 6 cut(s) 134, 167, 179, 206, 250, 314
CviQI GTAC 1 cut(s) 79
DdeI CTNAG 1 cut(s) 251
DpnI GATC 1 cut(s) 338
DpnII GATC 1 cut(s) 336
Eco47I GGWCC 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 172
FaeI CATG 3 cut(s) 99, 120, 310
FaiI YATR 5 cut(s) 43, 45, 97, 118, 308
FatI CATG 3 cut(s) 95, 116, 306
Fnu4HI GCNGC 1 cut(s) 49
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 1 cut(s) 135
GluI GCNGC 1 cut(s) 49
HapII CCGG 1 cut(s) 173
Hin1II CATG 3 cut(s) 99, 120, 310
HpaII CCGG 1 cut(s) 173
Hpy166II GTNNAC 1 cut(s) 148
Hpy188III TCNNGA 3 cut(s) 91, 325, 334
Hpy8I GTNNAC 1 cut(s) 148
Hpy99I CGWCG 2 cut(s) 197, 331
HpyCH4III ACNGT 2 cut(s) 67, 331
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 1 cut(s) 212
HpyF3I CTNAG 1 cut(s) 251
HpySE526I ACGT 1 cut(s) 195
Hsp92II CATG 3 cut(s) 99, 120, 310
Kzo9I GATC 1 cut(s) 336
LmnI GCTCC 1 cut(s) 176
LpnPI CCDG 2 cut(s) 186, 352
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 135
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 240
MalI GATC 1 cut(s) 338
MboI GATC 1 cut(s) 336
MhlI GDGCHC 1 cut(s) 259
MluCI AATT 1 cut(s) 274
MseI TTAA 2 cut(s) 15, 62
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 2 cut(s) 173, 174
MvnI CGCG 2 cut(s) 185, 326
MwoI GCNNNNNNNGC 1 cut(s) 212
NciI CCSGG 2 cut(s) 173, 174
NdeII GATC 1 cut(s) 336
NlaIII CATG 3 cut(s) 99, 120, 310
NmuCI GTSAC 1 cut(s) 240
NruI TCGCGA 1 cut(s) 326
NspI RCATGY 1 cut(s) 99
PciI ACATGT 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PkrI GCNGC 1 cut(s) 50
PscI ACATGT 1 cut(s) 95
PspPI GGNCC 1 cut(s) 296
RruI TCGCGA 1 cut(s) 326
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SaqAI TTAA 2 cut(s) 15, 62
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 1 cut(s) 336
Sau96I GGNCC 1 cut(s) 296
ScrFI CCNGG 2 cut(s) 173, 174
SduI GDGCHC 1 cut(s) 259
SetI ASST 5 cut(s) 136, 181, 198, 301, 316
SfaNI GCATC 1 cut(s) 96
SinI GGWCC 1 cut(s) 296
SmaI CCCGGG 1 cut(s) 174
Sse9I AATT 1 cut(s) 274
SsiI CCGC 2 cut(s) 183, 370
SspMI CTAG 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 171, 172
TaaI ACNGT 2 cut(s) 67, 331
TaiI ACGT 1 cut(s) 198
TasI AATT 1 cut(s) 274
TatI WGTACW 1 cut(s) 78
Tru1I TTAA 2 cut(s) 15, 62
Tru9I TTAA 2 cut(s) 15, 62
TseFI GTSAC 1 cut(s) 240
TseI GCWGC 1 cut(s) 48
Tsp45I GTSAC 1 cut(s) 240
TspDTI ATGAA 1 cut(s) 105
TspMI CCCGGG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 296
XceI RCATGY 1 cut(s) 99
XmaI CCCGGG 1 cut(s) 172
XspI CTAG 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.