FvH4_3g08440

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001932
Physical Location & Seq
Forward (+)
4924571 .. 4926099
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g08440.t1

Sequence Viewer

Length: 681 bp
ATGATGGATTTTAAGAAGGAGTTTATCGATGTGGTTCTGGTCCCAGCCGGGCTTCTGATCATGTTGATTTACCATCTCTTCCTCCTCTACAAGTATTTCCATCAACCTCTCTCCACATCCATGGGGTACGAAAACAACGACCAGCGAATTTGGGTTACAAAAATTCTTGAGGGTGACAATGTTAGCAGGGGACTTGGTGTGATTTCTTCTAATACAAGTGCAGCCATGACCTTAGCATCAATCTCTTTAACCCTCTCTTCCCTAATCGGAGTTTGGATATCAAATCTGCCTAGCAAGTTCTTCCCAATACAAATGATCTATGGCAATACTAGTCCATCGATGAATTCAATCAAGTACATCTGCCTGTTGACCTCTTTTCTGCTTGCATTTTCGTTCTTTGTTCAATCGGCTAGGCACTTCATCCATTCGAACTACCTGTTGAGCACTCCCGGGGTTAGCGATAAAAGGACTGTGAAGAAGGTGGAGAGAGCAGTTCAAAGAGGAAGTGAGTTTTGGGCGCTTGGGCTAAGAGCACTGTATTTCGGTCTTAATATTATGCTGTGGTTCTTTGGACCTATACCAATGTTTGCTTCCTCTGTCCTAATGGTGGTGATTCTGTATTGCCATGATATTAGGAAGTCGGAATATGATAATCTCCATGATGTACTATTGATTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

227

Amino Acids

25.77

Weight (kDa)

9.02

Isoelectric Point (pI)

29.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 12 - 213 1.8e-55 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 147, 162, 343
AfaI GTAC 3 cut(s) 128, 356, 666
AfiI CCNNNNNNNGG 1 cut(s) 607
AgsI TTSAA 3 cut(s) 348, 404, 497
AhlI ACTAGT 1 cut(s) 329
AjuI GAANNNNNNNTTGG 4 cut(s) 496, 528, 574, 606
Alw21I GWGCWC 2 cut(s) 446, 535
Ama87I CYCGRG 1 cut(s) 449
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 3 cut(s) 147, 162, 343
AspLEI GCGC 1 cut(s) 520
AspS9I GGNCC 2 cut(s) 40, 572
AsuC2I CCSGG 3 cut(s) 49, 450, 451
AsuHPI GGTGA 2 cut(s) 185, 622
AsuII TTCGAA 1 cut(s) 428
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 2 cut(s) 40, 572
Bbv12I GWGCWC 2 cut(s) 446, 535
BbvI GCAGC 1 cut(s) 233
BccI CCATC 3 cut(s) 81, 108, 343
BclI TGATCA 1 cut(s) 57
BcnI CCSGG 3 cut(s) 49, 450, 451
BcuI ACTAGT 1 cut(s) 329
BfaI CTAG 3 cut(s) 291, 330, 411
BfoI RGCGCY 1 cut(s) 521
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 3 cut(s) 49, 450, 451
Bme18I GGWCC 2 cut(s) 40, 572
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 40, 572
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 3 cut(s) 49, 450, 451
BmsI GCATC 1 cut(s) 245
Bpu10I CCTNAGC 1 cut(s) 232
Bpu14I TTCGAA 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 3 cut(s) 49, 450, 451
Bsa29I ATCGAT 2 cut(s) 27, 338
BsaJI CCNNGG 3 cut(s) 120, 449, 450
Bsc4I CCNNNNNNNGG 1 cut(s) 607
BseCI ATCGAT 2 cut(s) 27, 338
BseDI CCNNGG 3 cut(s) 120, 449, 450
BseGI GGATG 2 cut(s) 116, 420
BseLI CCNNNNNNNGG 1 cut(s) 607
BseRI GAGGAG 1 cut(s) 74
BseXI GCAGC 1 cut(s) 233
BseYI CCCAGC 1 cut(s) 43
BsgI GTGCAG 1 cut(s) 240
BshVI ATCGAT 2 cut(s) 27, 338
BsiHKAI GWGCWC 2 cut(s) 446, 535
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 2 cut(s) 48, 450
BslFI GGGAC 2 cut(s) 26, 204
BslI CCNNNNNNNGG 1 cut(s) 607
BsmFI GGGAC 2 cut(s) 26, 204
BsoBI CYCGRG 1 cut(s) 449
Bsp119I TTCGAA 1 cut(s) 428
Bsp1286I GDGCHC 2 cut(s) 446, 535
Bsp143I GATC 2 cut(s) 57, 315
Bsp19I CCATGG 1 cut(s) 120
BspDI ATCGAT 2 cut(s) 27, 338
BspLI GGNNCC 1 cut(s) 42
BspT104I TTCGAA 1 cut(s) 428
BssECI CCNNGG 3 cut(s) 120, 449, 450
BssMI GATC 2 cut(s) 57, 315
BssT1I CCWWGG 1 cut(s) 120
Bst4CI ACNGT 2 cut(s) 472, 537
Bst6I CTCTTC 2 cut(s) 83, 262
BstBI TTCGAA 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 2 cut(s) 232, 527
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 2 cut(s) 116, 420
BstH2I RGCGCY 1 cut(s) 521
BstHHI GCGC 1 cut(s) 520
BstKTI GATC 2 cut(s) 60, 318
BstMBI GATC 2 cut(s) 57, 315
BstSCI CCNGG 3 cut(s) 47, 448, 449
BstV1I GCAGC 1 cut(s) 233
BstXI CCANNNNNNTGG 1 cut(s) 121
Bsu15I ATCGAT 2 cut(s) 27, 338
BsuTUI ATCGAT 2 cut(s) 27, 338
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 2 cut(s) 116, 420
BtsIMutI CAGTG 1 cut(s) 533
Cac8I GCNNGC 1 cut(s) 384
CfoI GCGC 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 40, 572
Cfr9I CCCGGG 1 cut(s) 449
ClaI ATCGAT 2 cut(s) 27, 338
Csp6I GTAC 3 cut(s) 127, 355, 665
CviAII CATG 5 cut(s) 61, 121, 226, 626, 659
CviJI RGCY 5 cut(s) 47, 52, 224, 410, 526
CviKI_1 RGCY 5 cut(s) 47, 52, 224, 410, 526
CviQI GTAC 3 cut(s) 127, 355, 665
DdeI CTNAG 2 cut(s) 232, 527
DpnI GATC 2 cut(s) 59, 317
DpnII GATC 2 cut(s) 57, 315
Eam1104I CTCTTC 2 cut(s) 83, 262
EarI CTCTTC 2 cut(s) 83, 262
Eco130I CCWWGG 1 cut(s) 120
Eco32I GATATC 1 cut(s) 279
Eco47I GGWCC 2 cut(s) 40, 572
Eco88I CYCGRG 1 cut(s) 449
EcoRI GAATTC 1 cut(s) 343
EcoRV GATATC 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 5 cut(s) 64, 124, 229, 629, 662
FaiI YATR 9 cut(s) 62, 122, 227, 321, 557, 578, 627, 648, 660
FaqI GGGAC 2 cut(s) 26, 204
FatI CATG 5 cut(s) 60, 120, 225, 625, 658
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 2 cut(s) 103, 407
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 3 cut(s) 291, 330, 411
GlaI GCGC 1 cut(s) 519
GluI GCNGC 1 cut(s) 222
GsaI CCCAGC 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 521
HapII CCGG 2 cut(s) 48, 450
HhaI GCGC 1 cut(s) 520
Hin1II CATG 5 cut(s) 64, 124, 229, 629, 662
Hin6I GCGC 1 cut(s) 518
HinP1I GCGC 1 cut(s) 518
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HinfI GANTC 1 cut(s) 613
HpaII CCGG 2 cut(s) 48, 450
HphI GGTGA 2 cut(s) 185, 622
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 3 cut(s) 57, 269, 643
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 369
HpyAV CCTTC 2 cut(s) 10, 472
HpyCH4III ACNGT 2 cut(s) 472, 537
HpyCH4V TGCA 2 cut(s) 221, 386
HpyF3I CTNAG 2 cut(s) 232, 527
Hsp92II CATG 5 cut(s) 64, 124, 229, 629, 662
HspAI GCGC 1 cut(s) 518
Ksp22I TGATCA 1 cut(s) 57
Kzo9I GATC 2 cut(s) 57, 315
LpnPI CCDG 8 cut(s) 23, 57, 61, 155, 172, 377, 449, 463
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 1 cut(s) 245
MaeI CTAG 3 cut(s) 291, 330, 411
MaeIII GTNAC 2 cut(s) 154, 173
MalI GATC 2 cut(s) 59, 317
MboI GATC 2 cut(s) 57, 315
MboII GAAGA 5 cut(s) 70, 198, 249, 292, 487
MhlI GDGCHC 2 cut(s) 446, 535
MluCI AATT 3 cut(s) 147, 162, 343
MmeI TCCRAC 1 cut(s) 621
MnlI CCTC 8 cut(s) 92, 95, 117, 163, 263, 382, 494, 604
MseI TTAA 3 cut(s) 12, 248, 549
MslI CAYNNNNRTG 1 cut(s) 119
MspI CCGG 2 cut(s) 48, 450
MspR9I CCNGG 3 cut(s) 49, 450, 451
NciI CCSGG 3 cut(s) 49, 450, 451
NcoI CCATGG 1 cut(s) 120
NdeII GATC 2 cut(s) 57, 315
NlaIII CATG 5 cut(s) 64, 124, 229, 629, 662
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 173
NspV TTCGAA 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 135
PfeI GAWTC 1 cut(s) 613
PkrI GCNGC 1 cut(s) 223
PspFI CCCAGC 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 2 cut(s) 40, 572
RsaI GTAC 3 cut(s) 128, 356, 666
RsaNI GTAC 3 cut(s) 127, 355, 665
RseI CAYNNNNRTG 1 cut(s) 119
SaqAI TTAA 3 cut(s) 12, 248, 549
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 2 cut(s) 57, 315
Sau96I GGNCC 2 cut(s) 40, 572
ScrFI CCNGG 3 cut(s) 49, 450, 451
SduI GDGCHC 2 cut(s) 446, 535
SetI ASST 6 cut(s) 109, 233, 374, 438, 483, 577
SfaNI GCATC 1 cut(s) 245
SfuI TTCGAA 1 cut(s) 428
SinI GGWCC 2 cut(s) 40, 572
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 119
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
SpeI ACTAGT 1 cut(s) 329
Sse9I AATT 3 cut(s) 147, 162, 343
SspI AATATT 1 cut(s) 553
SspMI CTAG 3 cut(s) 291, 330, 411
StyD4I CCNGG 3 cut(s) 47, 448, 449
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 472, 537
TaqI TCGA 3 cut(s) 27, 338, 428
TaqII GACCGA 1 cut(s) 533
TasI AATT 3 cut(s) 147, 162, 343
TatI WGTACW 2 cut(s) 354, 664
TfiI GAWTC 1 cut(s) 613
Tru1I TTAA 3 cut(s) 12, 248, 549
Tru9I TTAA 3 cut(s) 12, 248, 549
TscAI CASTG 1 cut(s) 540
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 356, 409
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 1 cut(s) 540
VpaK11BI GGWCC 2 cut(s) 40, 572
XapI RAATTY 3 cut(s) 147, 162, 343
XmaI CCCGGG 1 cut(s) 449
XspI CTAG 3 cut(s) 291, 330, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.