MD05G1309300.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001932
Physical Location & Seq
Forward (+)
43992914 .. 43993261
348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1309300.v1.1.491

Sequence Viewer

Length: 348 bp
ATGAAAATGCACTTGGTTAATTACTATTCATTGCAGTCAATTACTATTCATTTGGGTAGGGGTGGAGTTGCTCCCAAAATACCTGTTGACTGCACCGGGAGCCACTGCGAAAAGAAGAAAGCCGAGAGAGAAGTTGAAAGGGGAAGTGAATTTTGGTCATTGGGTCTTAGAGCACTCTACTTTGCTCTCAATTTTCTGTTGTGGTTTTTTGGACCCATTCCCATGTTTACTTCGTCTGTCGCGACGGTCGTGATCCTAGCTTGCCACGATTTTAAGAACTCCGGCGGTGATCAAAAGTGCCCGGCGTCGGCCAATGCTAGAAATGAACGGTTATTGTTGGTTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

116

Amino Acids

12.79

Weight (kDa)

9.02

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 28 - 92 8.7e-19 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 242
AciI CCGC 1 cut(s) 285
AclWI GGATC 1 cut(s) 247
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 1 cut(s) 149
AcyI GRCGYC 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 307
AgsI TTSAA 1 cut(s) 137
AjuI GAANNNNNNNTTGG 3 cut(s) 28, 136, 168
AluBI AGCT 1 cut(s) 260
AluI AGCT 1 cut(s) 260
Alw21I GWGCWC 1 cut(s) 175
AlwI GGATC 1 cut(s) 247
AoxI GGCC 1 cut(s) 309
ApoI RAATTY 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 97, 302
AsuHPI GGTGA 1 cut(s) 299
AvaII GGWCC 1 cut(s) 212
BaeGI GKGCMC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 175
BclI TGATCA 1 cut(s) 289
BcnI CCSGG 2 cut(s) 97, 302
BfaI CTAG 2 cut(s) 257, 318
Bme1390I CCNGG 2 cut(s) 97, 302
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 1 cut(s) 212
BmiI GGNNCC 2 cut(s) 101, 214
BmrFI CCNGG 2 cut(s) 97, 302
BpuMI CCSGG 2 cut(s) 97, 302
BsaHI GRCGYC 1 cut(s) 305
Bsc4I CCNNNNNNNGG 1 cut(s) 307
Bse3DI GCAATG 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 307
BseMI GCAATG 1 cut(s) 29
BseSI GKGCMC 1 cut(s) 302
BsgI GTGCAG 1 cut(s) 76
Bsh1236I CGCG 1 cut(s) 242
Bsh1285I CGRYCG 1 cut(s) 249
BshFI GGCC 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 175
BsiSI CCGG 3 cut(s) 96, 282, 302
BslI CCNNNNNNNGG 1 cut(s) 307
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 175, 302
Bsp143I GATC 2 cut(s) 252, 289
Bsp68I TCGCGA 1 cut(s) 242
BspACI CCGC 1 cut(s) 285
BspANI GGCC 1 cut(s) 311
BspFNI CGCG 1 cut(s) 242
BspLI GGNNCC 2 cut(s) 101, 214
BspPI GGATC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 29
BssMI GATC 2 cut(s) 252, 289
BssNI GRCGYC 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 247, 330
BstACI GRCGYC 1 cut(s) 305
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 167
BstFNI CGCG 1 cut(s) 242
BstKTI GATC 2 cut(s) 255, 292
BstMBI GATC 2 cut(s) 252, 289
BstMCI CGRYCG 1 cut(s) 249
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 95, 300
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 1 cut(s) 242
BsuRI GGCC 1 cut(s) 311
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 103
BtuMI TCGCGA 1 cut(s) 242
Cac8I GCNNGC 1 cut(s) 262
Cfr13I GGNCC 1 cut(s) 212
CseI GACGC 1 cut(s) 294
CviAII CATG 1 cut(s) 223
CviJI RGCY 4 cut(s) 102, 122, 260, 311
CviKI_1 RGCY 4 cut(s) 102, 122, 260, 311
DdeI CTNAG 1 cut(s) 167
DpnI GATC 2 cut(s) 254, 291
DpnII GATC 2 cut(s) 252, 289
EaeI YGGCCR 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 212
FaeI CATG 1 cut(s) 226
FaiI YATR 1 cut(s) 224
FatI CATG 1 cut(s) 222
FbaI TGATCA 1 cut(s) 289
FspBI CTAG 2 cut(s) 257, 318
HaeIII GGCC 1 cut(s) 311
HapII CCGG 3 cut(s) 96, 282, 302
HgaI GACGC 1 cut(s) 294
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 1 cut(s) 226
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HpaII CCGG 3 cut(s) 96, 282, 302
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 2 cut(s) 88, 228
Hpy188III TCNNGA 3 cut(s) 241, 250, 345
Hpy8I GTNNAC 2 cut(s) 88, 228
Hpy99I CGWCG 2 cut(s) 247, 310
HpyCH4III ACNGT 2 cut(s) 247, 330
HpyCH4V TGCA 3 cut(s) 10, 34, 93
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 1 cut(s) 167
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 2 cut(s) 252, 289
LmnI GCTCC 2 cut(s) 76, 99
LpnPI CCDG 4 cut(s) 96, 109, 295, 315
MaeI CTAG 2 cut(s) 257, 318
MalI GATC 2 cut(s) 254, 291
MboI GATC 2 cut(s) 252, 289
MboII GAAGA 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 175, 302
MluCI AATT 4 cut(s) 19, 39, 149, 190
MseI TTAA 2 cut(s) 18, 273
MslI CAYNNNNRTG 1 cut(s) 221
MspI CCGG 3 cut(s) 96, 282, 302
MspR9I CCNGG 2 cut(s) 97, 302
MvnI CGCG 1 cut(s) 242
MwoI GCNNNNNNNGC 1 cut(s) 99
NciI CCSGG 2 cut(s) 97, 302
NdeII GATC 2 cut(s) 252, 289
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 2 cut(s) 101, 214
NmeAIII GCCGAG 1 cut(s) 148
NruI TCGCGA 1 cut(s) 242
PcsI WCGNNNNNNNCGW 2 cut(s) 239, 246
PspN4I GGNNCC 2 cut(s) 101, 214
PspPI GGNCC 1 cut(s) 212
RruI TCGCGA 1 cut(s) 242
RseI CAYNNNNRTG 1 cut(s) 221
SaqAI TTAA 2 cut(s) 18, 273
Sau3AI GATC 2 cut(s) 252, 289
Sau96I GGNCC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 97, 302
SduI GDGCHC 2 cut(s) 175, 302
SetI ASST 2 cut(s) 85, 262
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 221
Sse9I AATT 4 cut(s) 19, 39, 149, 190
SsiI CCGC 1 cut(s) 285
SspMI CTAG 2 cut(s) 257, 318
StyD4I CCNGG 2 cut(s) 95, 300
TaaI ACNGT 2 cut(s) 247, 330
TasI AATT 4 cut(s) 19, 39, 149, 190
Tru1I TTAA 2 cut(s) 18, 273
Tru9I TTAA 2 cut(s) 18, 273
TscAI CASTG 1 cut(s) 110
TspDTI ATGAA 4 cut(s) 17, 18, 38, 339
TspRI CASTG 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 212
XapI RAATTY 1 cut(s) 149
XspI CTAG 2 cut(s) 257, 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.