Rw5G006860

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001932
Physical Location & Seq
Reverse (-)
7083816 .. 7087339
3524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G006860.1

Sequence Viewer

Length: 696 bp
ATGAATATTGAGGAGGAGTACATAGATGTGCTTTTGGTCCCTGCTGGGTTACTGATCATGCTAGTTTACCACCTCTTCCTCCTTTTCAAGTACTTCAATAAGCCTCTCTCCACATCCATAGGATACGAAAACAACGACAAACGAATTTGGGCTGCAAAAATCTTGCAGGGTACTAATGATGATGCAGTTGGGGACAATGTTAGCACAGGACTTAGTGTGATTTCCTCAAACACAAGCGCAACCATGACCTTAGCGTCAATCTCCTTAACTCTCTGCGCTGTTATCGGAGTCTGGATTGCAAGTTCAACCAATAAGTTCTTCCCATTAGAAATGGTTTACGGCAACACAAGCGAGTCAATCATTTCTATCAAGTACTTATGCCTGTTGAGCTGCTTTCTCCTTGCCTTTTCGTTCTTCGTTCAATCTGCGAGGCACTTTGTCCATTCAAACTACCTATTGAGCACTCCAGGAGCCACGAAAGAAGAGGATGTGGAAAAAGTGCAGAAAGCGGTTGAAAGAGGAAGTATCTTTTGGTCATTGGGACTCAGAGCACTATATTTTGCTCTTAATTTTCTGCTGTGGTTCTTCGGGCCTATACCAATGTGTGCTTGCTCTATTGTAATGGTATTTGTCCTGTACTGCCATGATTTCAGGAAGGACTCGAAACAGGGGAGTTACAAGGTATACAAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

231

Amino Acids

26.01

Weight (kDa)

7.58

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 12 - 219 4.6e-57 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 253
AccI GTMKAC 1 cut(s) 684
AciI CCGC 1 cut(s) 509
AcsI RAATTY 1 cut(s) 144
AfaI GTAC 5 cut(s) 20, 92, 172, 374, 638
AgsI TTSAA 6 cut(s) 88, 97, 306, 422, 447, 515
AjnI CCWGG 1 cut(s) 466
AjuI GAANNNNNNNTTGG 4 cut(s) 302, 334, 514, 546
AloI GAACNNNNNNTCC 2 cut(s) 286, 318
AluBI AGCT 1 cut(s) 390
AluI AGCT 1 cut(s) 390
Alw21I GWGCWC 2 cut(s) 464, 553
AoxI GGCC 1 cut(s) 590
ApeKI GCWGC 2 cut(s) 152, 390
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 2 cut(s) 239, 278
AspS9I GGNCC 2 cut(s) 37, 590
AvaII GGWCC 1 cut(s) 37
Bbv12I GWGCWC 2 cut(s) 464, 553
BbvI GCAGC 2 cut(s) 139, 377
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 1 cut(s) 468
BciVI GTATCC 1 cut(s) 116
BclI TGATCA 1 cut(s) 54
BfaI CTAG 1 cut(s) 62
BfuI GTATCC 1 cut(s) 116
BisI GCNGC 2 cut(s) 153, 391
BlsI GCNGC 2 cut(s) 154, 392
BmcAI AGTACT 2 cut(s) 92, 374
Bme1390I CCNGG 1 cut(s) 468
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 2 cut(s) 37, 590
BmiI GGNNCC 2 cut(s) 39, 472
BmrFI CCNGG 1 cut(s) 468
BmsI GCATC 1 cut(s) 172
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 1 cut(s) 250
BseBI CCWGG 1 cut(s) 468
BseGI GGATG 2 cut(s) 113, 493
BseMII CTCAG 1 cut(s) 559
BseRI GAGGAG 2 cut(s) 26, 29
BseXI GCAGC 2 cut(s) 139, 377
BseYI CCCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 521
BshFI GGCC 1 cut(s) 592
BsiHKAI GWGCWC 2 cut(s) 464, 553
BslFI GGGAC 3 cut(s) 23, 206, 555
BsmFI GGGAC 3 cut(s) 23, 206, 555
BsnI GGCC 1 cut(s) 592
Bsp1286I GDGCHC 2 cut(s) 464, 553
Bsp143I GATC 1 cut(s) 54
BspACI CCGC 1 cut(s) 509
BspANI GGCC 1 cut(s) 592
BspCNI CTCAG 1 cut(s) 558
BspLI GGNNCC 2 cut(s) 39, 472
BssMI GATC 1 cut(s) 54
BssNAI GTATAC 1 cut(s) 685
Bst1107I GTATAC 1 cut(s) 685
Bst2UI CCWGG 1 cut(s) 468
Bst6I CTCTTC 2 cut(s) 80, 477
BstC8I GCNNGC 1 cut(s) 610
BstDEI CTNAG 3 cut(s) 212, 250, 545
BstF5I GGATG 2 cut(s) 113, 493
BstHHI GCGC 2 cut(s) 239, 278
BstKTI GATC 1 cut(s) 57
BstMBI GATC 1 cut(s) 54
BstMWI GCNNNNNNNGC 2 cut(s) 348, 387
BstNI CCWGG 1 cut(s) 468
BstSCI CCNGG 1 cut(s) 466
BstV1I GCAGC 2 cut(s) 139, 377
BstZ17I GTATAC 1 cut(s) 685
BsuI GTATCC 1 cut(s) 116
BsuRI GGCC 1 cut(s) 592
BtsCI GGATG 2 cut(s) 113, 493
Cac8I GCNNGC 1 cut(s) 610
CfoI GCGC 2 cut(s) 239, 278
Cfr13I GGNCC 2 cut(s) 37, 590
CseI GACGC 1 cut(s) 243
Csp6I GTAC 5 cut(s) 19, 91, 171, 373, 637
CviAII CATG 3 cut(s) 58, 244, 644
CviJI RGCY 5 cut(s) 103, 152, 390, 473, 592
CviKI_1 RGCY 5 cut(s) 103, 152, 390, 473, 592
CviQI GTAC 5 cut(s) 19, 91, 171, 373, 637
DdeI CTNAG 3 cut(s) 212, 250, 545
DpnI GATC 1 cut(s) 56
DpnII GATC 1 cut(s) 54
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
Eam1104I CTCTTC 2 cut(s) 80, 477
EarI CTCTTC 2 cut(s) 80, 477
Eco47I GGWCC 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 466
FaeI CATG 3 cut(s) 61, 247, 647
FaiI YATR 9 cut(s) 23, 59, 119, 245, 379, 556, 596, 645, 685
FaqI GGGAC 3 cut(s) 23, 206, 555
FatI CATG 3 cut(s) 57, 243, 643
FbaI TGATCA 1 cut(s) 54
FblI GTMKAC 1 cut(s) 684
Fnu4HI GCNGC 2 cut(s) 153, 391
FokI GGATG 2 cut(s) 100, 500
Fsp4HI GCNGC 2 cut(s) 153, 391
FspBI CTAG 1 cut(s) 62
GlaI GCGC 2 cut(s) 238, 277
GluI GCNGC 2 cut(s) 153, 391
GsaI CCCAGC 1 cut(s) 48
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 1 cut(s) 592
HgaI GACGC 1 cut(s) 243
HhaI GCGC 2 cut(s) 239, 278
Hin1II CATG 3 cut(s) 61, 247, 647
Hin6I GCGC 2 cut(s) 237, 276
HinP1I GCGC 2 cut(s) 237, 276
HinfI GANTC 4 cut(s) 288, 353, 543, 659
Hpy166II GTNNAC 3 cut(s) 67, 337, 685
Hpy188I TCNGA 2 cut(s) 287, 548
Hpy188III TCNNGA 2 cut(s) 292, 652
Hpy8I GTNNAC 3 cut(s) 67, 337, 685
HpyAV CCTTC 1 cut(s) 649
HpyCH4V TGCA 5 cut(s) 155, 166, 185, 299, 502
HpyF10VI GCNNNNNNNGC 2 cut(s) 348, 387
HpyF3I CTNAG 3 cut(s) 212, 250, 545
Hsp92II CATG 3 cut(s) 61, 247, 647
HspAI GCGC 2 cut(s) 237, 276
Ksp22I TGATCA 1 cut(s) 54
Kzo9I GATC 1 cut(s) 54
LmnI GCTCC 1 cut(s) 470
Lsp1109I GCAGC 2 cut(s) 139, 377
LweI GCATC 1 cut(s) 172
MaeI CTAG 1 cut(s) 62
MaeIII GTNAC 2 cut(s) 48, 674
MalI GATC 1 cut(s) 56
MboI GATC 1 cut(s) 54
MboII GAAGA 5 cut(s) 67, 310, 406, 494, 577
MhlI GDGCHC 2 cut(s) 464, 553
MluCI AATT 2 cut(s) 144, 568
MlyI GAGTC 4 cut(s) 297, 362, 537, 653
MnlI CCTC 9 cut(s) 4, 7, 83, 89, 114, 235, 423, 478, 512
MseI TTAA 2 cut(s) 266, 567
MslI CAYNNNNRTG 1 cut(s) 26
MspR9I CCNGG 1 cut(s) 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 2 cut(s) 348, 387
NdeII GATC 1 cut(s) 54
NlaIII CATG 3 cut(s) 61, 247, 647
NlaIV GGNNCC 2 cut(s) 39, 472
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PfoI TCCNGGA 1 cut(s) 466
PkrI GCNGC 2 cut(s) 154, 392
PleI GAGTC 4 cut(s) 296, 361, 537, 653
PpsI GAGTC 4 cut(s) 296, 361, 537, 653
Psp6I CCWGG 1 cut(s) 466
PspFI CCCAGC 1 cut(s) 44
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 2 cut(s) 39, 472
PspPI GGNCC 2 cut(s) 37, 590
RsaI GTAC 5 cut(s) 20, 92, 172, 374, 638
RsaNI GTAC 5 cut(s) 19, 91, 171, 373, 637
RseI CAYNNNNRTG 1 cut(s) 26
SaqAI TTAA 2 cut(s) 266, 567
SatI GCNGC 2 cut(s) 153, 391
Sau3AI GATC 1 cut(s) 54
Sau96I GGNCC 2 cut(s) 37, 590
ScaI AGTACT 2 cut(s) 92, 374
SchI GAGTC 4 cut(s) 297, 362, 537, 653
ScrFI CCNGG 1 cut(s) 468
SduI GDGCHC 2 cut(s) 464, 553
SetI ASST 6 cut(s) 75, 251, 392, 456, 684, 695
SfaNI GCATC 1 cut(s) 172
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 1 cut(s) 26
Sse9I AATT 2 cut(s) 144, 568
SsiI CCGC 1 cut(s) 509
SspI AATATT 1 cut(s) 7
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 466
TaqI TCGA 1 cut(s) 662
TasI AATT 2 cut(s) 144, 568
TatI WGTACW 4 cut(s) 18, 90, 372, 636
Tru1I TTAA 2 cut(s) 266, 567
Tru9I TTAA 2 cut(s) 266, 567
TseI GCWGC 2 cut(s) 152, 390
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 684
XspI CTAG 1 cut(s) 62
ZrmI AGTACT 2 cut(s) 92, 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.