MD10G1287800.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001932
Physical Location & Seq
Forward (+)
37688897 .. 37689871
975 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1287800.v1.1.491

Sequence Viewer

Length: 729 bp
ATGGCTTTTCTGCATTTTCTGGAGGAGTACTTAGACGTGGTGTTGGTCCCAACCGGGATCTTGATCATGCTAATCTACCACCTCTTCCTCCTCTACAAATACATCAATCACCCTCTCGCCACCGCTATTGGATCTGAAAACAACGACAAGAAAACTTGGGTTGGAAAAATCTTGCATGGTAAAGATGTCGAAGTTGCAAAGAAAGTTAAAACAGCTATTGATGTGATTTCCTCCAATACAAGTGCAACTACGACCTTAGCATCAATCTCTCTAACTCTCTGCTCTCTCATCGGAGCTTGGATGGCAAACTCCAACAACTTCTTCCCAAAAGCAATAATATATGGCAACACAAACCCTACAACTATTTCCATCAAGTACATCTGCCTCTTGACATGCTTTCTCCTTGCTTTTTCATGCTTTGTTCAATCGGCCAGGCACTTTGTCCACTCAAACTACCTGTTAACCACGCCAGGAGCTACTGCCGAAAGCGACGTGAAGAAAGCCAAGAAAGCAGTTCAAAGGGGAAGTGAGTTTTGGTCATTGGGTCTTCGAGCACTCTACTTTGCTCTCAATTTTCTGTTGTGGTTTTTTGGACCAATTCCCATGTTTGCTTCGTCTGTCGTAACAGTCGTGATCCTATCATGCCACGACTTTAAGAAGTCGTGCGGCGACCAAAAGTGGCCGGCGTCGGCCAAGGCTAGGAGTGAACATTTATTATCGTTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

243

Amino Acids

26.87

Weight (kDa)

9.18

Isoelectric Point (pI)

23.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 14 - 220 1.6e-58 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 123, 666
AclWI GGATC 3 cut(s) 65, 139, 628
AcoI YGGCCR 3 cut(s) 429, 680, 690
AcyI GRCGYC 1 cut(s) 686
AfaI GTAC 2 cut(s) 29, 377
AfiI CCNNNNNNNGG 1 cut(s) 699
AgsI TTSAA 2 cut(s) 425, 518
AjiI CACGTC 2 cut(s) 37, 493
AjnI CCWGG 2 cut(s) 431, 469
AjuI GAANNNNNNNTTGG 4 cut(s) 305, 337, 517, 549
AluBI AGCT 3 cut(s) 215, 296, 476
AluI AGCT 3 cut(s) 215, 296, 476
Alw21I GWGCWC 1 cut(s) 556
AlwI GGATC 3 cut(s) 65, 139, 628
AoxI GGCC 3 cut(s) 429, 680, 690
AspS9I GGNCC 2 cut(s) 46, 593
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 101
AvaII GGWCC 2 cut(s) 46, 593
BbsI GAAGAC 1 cut(s) 539
Bbv12I GWGCWC 1 cut(s) 556
BccI CCATC 2 cut(s) 295, 377
BciT130I CCWGG 2 cut(s) 433, 471
BclI TGATCA 1 cut(s) 63
BcnI CCSGG 1 cut(s) 55
BfaI CTAG 1 cut(s) 699
BisI GCNGC 1 cut(s) 667
BlsI GCNGC 1 cut(s) 668
BmcAI AGTACT 1 cut(s) 29
Bme1390I CCNGG 3 cut(s) 55, 433, 471
Bme18I GGWCC 2 cut(s) 46, 593
BmgBI CACGTC 2 cut(s) 37, 493
BmgT120I GGNCC 2 cut(s) 46, 593
BmiI GGNNCC 1 cut(s) 48
BmrFI CCNGG 3 cut(s) 55, 433, 471
BmsI GCATC 1 cut(s) 269
BpiI GAAGAC 1 cut(s) 539
BpmI CTGGAG 1 cut(s) 41
Bpu10I CCTNAGC 1 cut(s) 256
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 62
BsaHI GRCGYC 1 cut(s) 686
BsaJI CCNNGG 1 cut(s) 693
Bsc4I CCNNNNNNNGG 1 cut(s) 699
Bse118I RCCGGY 1 cut(s) 682
Bse8I GATNNNNATC 1 cut(s) 62
BseBI CCWGG 2 cut(s) 433, 471
BseDI CCNNGG 1 cut(s) 693
BseGI GGATG 1 cut(s) 306
BseJI GATNNNNATC 1 cut(s) 62
BseLI CCNNNNNNNGG 1 cut(s) 699
BseRI GAGGAG 2 cut(s) 38, 80
BshFI GGCC 3 cut(s) 431, 682, 692
BsiHKAI GWGCWC 1 cut(s) 556
BsiSI CCGG 2 cut(s) 54, 683
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 699
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 3 cut(s) 431, 682, 692
Bsp1286I GDGCHC 1 cut(s) 556
Bsp143I GATC 4 cut(s) 57, 63, 131, 633
BspACI CCGC 2 cut(s) 123, 666
BspANI GGCC 3 cut(s) 431, 682, 692
BspLI GGNNCC 1 cut(s) 48
BspPI GGATC 3 cut(s) 65, 139, 628
BsrFI RCCGGY 1 cut(s) 682
BssAI RCCGGY 1 cut(s) 682
BssECI CCNNGG 1 cut(s) 693
BssMI GATC 4 cut(s) 57, 63, 131, 633
BssNI GRCGYC 1 cut(s) 686
BssT1I CCWWGG 1 cut(s) 693
Bst2UI CCWGG 2 cut(s) 433, 471
Bst4CI ACNGT 1 cut(s) 628
Bst6I CTCTTC 1 cut(s) 89
BstACI GRCGYC 1 cut(s) 686
BstC8I GCNNGC 1 cut(s) 684
BstDEI CTNAG 2 cut(s) 31, 256
BstF5I GGATG 1 cut(s) 306
BstKTI GATC 4 cut(s) 60, 66, 134, 636
BstMBI GATC 4 cut(s) 57, 63, 131, 633
BstMWI GCNNNNNNNGC 2 cut(s) 302, 509
BstNI CCWGG 2 cut(s) 433, 471
BstNSI RCATGY 1 cut(s) 396
BstSCI CCNGG 3 cut(s) 53, 431, 469
BstV2I GAAGAC 1 cut(s) 539
BstX2I RGATCY 2 cut(s) 57, 131
BstYI RGATCY 2 cut(s) 57, 131
BsuRI GGCC 3 cut(s) 431, 682, 692
BtrI CACGTC 2 cut(s) 37, 493
BtsCI GGATG 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 684
Cfr10I RCCGGY 1 cut(s) 682
Cfr13I GGNCC 2 cut(s) 46, 593
CseI GACGC 1 cut(s) 675
Csp6I GTAC 2 cut(s) 28, 376
CspCI CAANNNNNGTGG 2 cut(s) 109, 144
CviAII CATG 6 cut(s) 67, 176, 393, 414, 604, 642
CviJI RGCY 9 cut(s) 5, 215, 296, 431, 476, 503, 682, 692, 698
CviKI_1 RGCY 9 cut(s) 5, 215, 296, 431, 476, 503, 682, 692, 698
CviQI GTAC 2 cut(s) 28, 376
DdeI CTNAG 2 cut(s) 31, 256
DpnI GATC 4 cut(s) 59, 65, 133, 635
DpnII GATC 4 cut(s) 57, 63, 131, 633
EaeI YGGCCR 3 cut(s) 429, 680, 690
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 693
Eco47I GGWCC 2 cut(s) 46, 593
EcoRII CCWGG 2 cut(s) 431, 469
EcoT14I CCWWGG 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 6 cut(s) 70, 179, 396, 417, 607, 645
FaiI YATR 8 cut(s) 68, 177, 340, 342, 394, 415, 605, 643
FaqI GGGAC 1 cut(s) 32
FatI CATG 6 cut(s) 66, 175, 392, 413, 603, 641
FbaI TGATCA 1 cut(s) 63
Fnu4HI GCNGC 1 cut(s) 667
FokI GGATG 1 cut(s) 313
Fsp4HI GCNGC 1 cut(s) 667
FspBI CTAG 1 cut(s) 699
GluI GCNGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 41
HaeIII GGCC 3 cut(s) 431, 682, 692
HapII CCGG 2 cut(s) 54, 683
HgaI GACGC 1 cut(s) 675
Hin1I GRCGYC 1 cut(s) 686
Hin1II CATG 6 cut(s) 70, 179, 396, 417, 607, 645
HincII GTYRAC 1 cut(s) 462
HindII GTYRAC 1 cut(s) 462
HpaI GTTAAC 1 cut(s) 462
HpaII CCGG 2 cut(s) 54, 683
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 3 cut(s) 445, 462, 707
Hpy188I TCNGA 2 cut(s) 136, 293
Hpy188III TCNNGA 5 cut(s) 20, 61, 388, 631, 726
Hpy8I GTNNAC 3 cut(s) 445, 462, 707
Hpy99I CGWCG 2 cut(s) 494, 691
HpyCH4III ACNGT 1 cut(s) 628
HpyCH4IV ACGT 2 cut(s) 36, 492
HpyCH4V TGCA 4 cut(s) 13, 175, 197, 245
HpyF10VI GCNNNNNNNGC 2 cut(s) 302, 509
HpyF3I CTNAG 2 cut(s) 31, 256
HpySE526I ACGT 2 cut(s) 36, 492
Hsp92I GRCGYC 1 cut(s) 686
Hsp92II CATG 6 cut(s) 70, 179, 396, 417, 607, 645
KroI GCCGGC 1 cut(s) 682
KroNI GCCGGC 1 cut(s) 684
Ksp22I TGATCA 1 cut(s) 63
KspAI GTTAAC 1 cut(s) 462
Kzo9I GATC 4 cut(s) 57, 63, 131, 633
LmnI GCTCC 2 cut(s) 293, 473
LpnPI CCDG 8 cut(s) 5, 67, 418, 445, 456, 470, 483, 696
LweI GCATC 1 cut(s) 269
MaeI CTAG 1 cut(s) 699
MaeII ACGT 2 cut(s) 36, 492
MaeIII GTNAC 1 cut(s) 622
MalI GATC 4 cut(s) 59, 65, 133, 635
MboI GATC 4 cut(s) 57, 63, 131, 633
MboII GAAGA 4 cut(s) 76, 313, 508, 539
MflI RGATCY 2 cut(s) 57, 131
MhlI GDGCHC 1 cut(s) 556
MluCI AATT 2 cut(s) 571, 597
MmeI TCCRAC 2 cut(s) 142, 336
MnlI CCTC 7 cut(s) 16, 92, 98, 101, 123, 241, 395
MroNI GCCGGC 1 cut(s) 682
MseI TTAA 3 cut(s) 207, 461, 654
MspI CCGG 2 cut(s) 54, 683
MspR9I CCNGG 3 cut(s) 55, 433, 471
MvaI CCWGG 2 cut(s) 433, 471
MwoI GCNNNNNNNGC 2 cut(s) 302, 509
NaeI GCCGGC 1 cut(s) 684
NciI CCSGG 1 cut(s) 55
NdeII GATC 4 cut(s) 57, 63, 131, 633
NgoMIV GCCGGC 1 cut(s) 682
NlaIII CATG 6 cut(s) 70, 179, 396, 417, 607, 645
NlaIV GGNNCC 1 cut(s) 48
NspI RCATGY 1 cut(s) 396
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PdiI GCCGGC 1 cut(s) 684
PkrI GCNGC 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 431, 469
PspGI CCWGG 2 cut(s) 431, 469
PspN4I GGNNCC 1 cut(s) 48
PspPI GGNCC 2 cut(s) 46, 593
PsuI RGATCY 2 cut(s) 57, 131
RsaI GTAC 2 cut(s) 29, 377
RsaNI GTAC 2 cut(s) 28, 376
SaqAI TTAA 3 cut(s) 207, 461, 654
SatI GCNGC 1 cut(s) 667
Sau3AI GATC 4 cut(s) 57, 63, 131, 633
Sau96I GGNCC 2 cut(s) 46, 593
ScaI AGTACT 1 cut(s) 29
ScrFI CCNGG 3 cut(s) 55, 433, 471
SduI GDGCHC 1 cut(s) 556
SetI ASST 8 cut(s) 39, 84, 217, 257, 298, 459, 478, 495
SfaNI GCATC 1 cut(s) 269
SinI GGWCC 2 cut(s) 46, 593
Sse9I AATT 2 cut(s) 571, 597
SsiI CCGC 2 cut(s) 123, 666
SspMI CTAG 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 53, 431, 469
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 1 cut(s) 628
TaiI ACGT 2 cut(s) 39, 495
TaqI TCGA 2 cut(s) 189, 550
TasI AATT 2 cut(s) 571, 597
TatI WGTACW 2 cut(s) 27, 375
TauI GCSGC 1 cut(s) 669
Tru1I TTAA 3 cut(s) 207, 461, 654
Tru9I TTAA 3 cut(s) 207, 461, 654
TspDTI ATGAA 1 cut(s) 402
VpaK11BI GGWCC 2 cut(s) 46, 593
XceI RCATGY 1 cut(s) 396
XspI CTAG 1 cut(s) 699
ZrmI AGTACT 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.