Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0001862

Physical Location & Seq

Genomic Coordinates Reverse (-)

16203139 .. 16208079

Gene Structure 4941 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g23140.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGGAAGCCAAACTTAAGTCTGTTACCGCGACGCTGTATCTCTGTTTCCTCTTGTTTCCCTTGGTTTTTTGTGCATCCAATGATGGATTGCTCCGTGTTGGACTGAAAAAGAAGAAGTTTGATCAGAATAACCGGGTTGCTGCTAATCTCCAGGCCAAGAATGGAGATGCTGTGAGAGCTGTTATCCACAAATATAATCTTCGTGGAACCTATGGAGATGACCAGGACATTGATATTGTGTCTCTCAAGAATTACATGGATGCTCAGTACTTTGGGGAGATTGGTATTGGTACTCCTCCTCAGAAGTTCACTGTGATCTTTGACACTGGTAGCTCAAATTTGTGGGTGCCTTCTTCCAAGTGTTATTTCTCGATTGCCTGCTATCTACACCCAAAGTACAAATCAAGCAGTTCAAGCACCTATAGCAAAAATGGTAAGCCTGCAGCAATCCAGTATGGAACTGGTGCAATCTCTGGCTTCTTTAGTGAAGACCATGTTACAGTTGGTGATCTTGTGGTGCAAGATCAGGAATTTATCGAGGCGACCAAGGAGCCTGGCATCACATTCTTGGTAGCCAAATTTGATGGCATACTTGGCCTTGGATTTCAAGAAATTTCAGTTGGAAATGCTGTTCCTGTCTGGTATAACATGGTTAAGCAGGGTCTTGTTAAGGAAGCTGTTTTCTCATTCTGGTTTAACCGCAATGCTGACGAAGAAGAAGGAGGTGAACTTGTCTTTGGTGGAGTGGATCCTAATCATTATGTCGGAGAGCACACATATGTTCCTGTTACTCAGAAGGGCTATTGGCAGTTTGACATGGGTGATGTTTTGATCGACGGTAAAACAACTGGATTTTGTGCCGGTGGATGTGCAGCAATTGCTGATTCTGGAACTTCTCTGTTGGTTGGCCCAACGACAATTATTACTGAACTCAACCATGCCATTGGAGCCACTGGCATTGTGAGTCAAGAATGCAAGACTGTGGTTGCAGAATATGGAGATACCATAATAAAGATGATTTTAGCAAAGGACCAACCACAGAAGATCTGTTCTCAGATTGGGTTATGCACATTTGATGGGACTCGTGGTGTAAGTGTTGGAATCAAGAGTGTTGTGGATGAGAACAATCACAAGTCATCTGCTGGCTTATCTGATGCTATGTGTTCTGCTTGTGAGATGACTGTTGTATGGATGCAAAATCAGCTCAAGCAGAATCAAACACAGGATCGCATACTTGACTATGTGAATCAGCTCTGTGACCGGCTGCCTAGCCCAATGGGAGAATCTGCTGTTGATTGTGCTGGTTTGTCTTCCATGCCTAGTGTTTCCTTCACAATTGGTGGAAAGCAATTTGATCTAGCCCCTGAGCAGTATGTGCTCAAAGTAGGCGAGGGTGAGGTAGCACAATGCATCAGTGGATTTACTGCTTTGGATGTACCACCTCCTCGTGGACCACTCTGGATTCTGGGAGATGTGTTCATGGGTCAGTACCACACAGTGTTTGACTTTGGCAAGGAGAGAATTGGATTTGCAGAAGCTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

515

Amino Acids

55.73

Weight (kDa)

5.04

Isoelectric Point (pI)

31.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	89 - 513	1e-117	Eukaryotic aspartyl protease
TAXi_N	PF14543	90 - 248	1.8e-11	Xylanase inhibitor N-terminal
SapB_2	PF03489	323 - 356	2.2e-12	Saposin-like type B, region 2
SapB_1	PF05184	387 - 423	5.7e-14	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11910 AT1G11910 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290
fragaria_vesca	FvH4_3g23140 FvH4_3g23140 FvH4_6g42880 FvH4_6g42880
malus_domestica	MD00G1159300.v1.1 MD09G1111100.v1.1 MD11G1213600.v1.1 MD15G1413400.v1.1 MD17G1098900.v1.1 MD17G1282600.v1.1 MD17G1282700.v1.1
prunus_persica	Prupe.3G214900_v2.0.a1 Prupe.3G214900_v2.0.a1 Prupe.4G217700_v2.0.a1
pyrus_communis	pycom03g14920 pycom09g03360 pycom11g18820 pycom17g09430
rosa_chinensis	RchiOBHm_Chr2g0158811 RchiOBHm_Chr5g0040781
rosa_laevigata	RLG00000021115 RLG00000034001
rosa_multiflora	Rmu_sc0001579.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039910 Rroxscaffold_2G00090980
rosa_rugosa	Rorug02G0472700 Rorug05G0187200 Rorug05G0187300
rosa_samantha	Rh2AG538500 Rh2BG551200 Rh2CG521800 Rh2DG561000 Rh5AG273000 Rh5BG278100 Rh5CG310100 Rh5DG286000
rosa_wichuraiana	Rw2G044570 Rw5G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	346
AccII	CGCG	1 cut(s)	29
AciI	CCGC	2 cut(s)	27, 700
AclWI	GGATC	3 cut(s)	743, 756, 1233
AcsI	RAATTY	4 cut(s)	337, 530, 578, 612
AdeI	CACNNNGTG	1 cut(s)	1498
AfaI	GTAC	5 cut(s)	269, 292, 398, 1437, 1490
AfiI	CCNNNNNNNGG	1 cut(s)	1448
AflII	CTTAAG	1 cut(s)	14
AgsI	TTSAA	2 cut(s)	414, 608
AjnI	CCWGG	3 cut(s)	150, 222, 553
AjuI	GAANNNNNNNTTGG	6 cut(s)	350, 382, 603, 635, 720, 752
AloI	GAACNNNNNNTCC	2 cut(s)	615, 647
AluBI	AGCT	6 cut(s)	179, 333, 677, 1204, 1252, 1538
AluI	AGCT	6 cut(s)	179, 333, 677, 1204, 1252, 1538
Alw21I	GWGCWC	2 cut(s)	774, 1380
Alw26I	GTCTC	1 cut(s)	246
AlwI	GGATC	3 cut(s)	743, 756, 1233
AlwNI	CAGNNNCTG	1 cut(s)	1538
AoxI	GGCC	3 cut(s)	153, 595, 907
ApeKI	GCWGC	5 cut(s)	140, 443, 872, 1264, 1538
ApoI	RAATTY	4 cut(s)	337, 530, 578, 612
AspS9I	GGNCC	3 cut(s)	908, 1030, 1451
AsuC2I	CCSGG	1 cut(s)	134
AsuHPI	GGTGA	4 cut(s)	518, 737, 833, 1406
AvaII	GGWCC	2 cut(s)	1030, 1451
BamHI	GGATCC	1 cut(s)	748
BanI	GGYRCC	1 cut(s)	346
BauI	CACGAG	2 cut(s)	1083, 1446
BbsI	GAAGAC	2 cut(s)	495, 1302
Bbv12I	GWGCWC	2 cut(s)	774, 1380
BbvI	GCAGC	5 cut(s)	127, 455, 884, 1251, 1525
BccI	CCATC	3 cut(s)	77, 578, 1070
BciT130I	CCWGG	3 cut(s)	152, 224, 555
BclI	TGATCA	1 cut(s)	121
BcnI	CCSGG	1 cut(s)	134
BcoDI	GTCTC	1 cut(s)	246
BfaI	CTAG	3 cut(s)	1269, 1320, 1358
BfmI	CTRYAG	2 cut(s)	421, 441
BfrI	CTTAAG	1 cut(s)	14
BglII	AGATCT	1 cut(s)	1044
BisI	GCNGC	5 cut(s)	141, 444, 873, 1265, 1539
BlsI	GCNGC	5 cut(s)	142, 445, 874, 1266, 1540
BmcAI	AGTACT	1 cut(s)	269
Bme1390I	CCNGG	4 cut(s)	134, 152, 224, 555
Bme18I	GGWCC	2 cut(s)	1030, 1451
BmgT120I	GGNCC	3 cut(s)	908, 1030, 1451
BmiI	GGNNCC	5 cut(s)	208, 348, 552, 750, 949
BmrFI	CCNGG	4 cut(s)	134, 152, 224, 555
BmsI	GCATC	7 cut(s)	83, 157, 250, 567, 1144, 1182, 1419
BpiI	GAAGAC	2 cut(s)	495, 1302
BpmI	CTGGAG	1 cut(s)	134
Bpu10I	CCTNAGC	1 cut(s)	1365
BpuEI	CTTGAG	2 cut(s)	230, 1190
BpuMI	CCSGG	1 cut(s)	134
BsaBI	GATNNNNATC	1 cut(s)	753
BsaJI	CCNNGG	3 cut(s)	60, 546, 598
BsaXI	ACNNNNNCTCC	2 cut(s)	156, 186
Bsc4I	CCNNNNNNNGG	1 cut(s)	1448
Bse118I	RCCGGY	2 cut(s)	860, 1260
Bse1I	ACTGG	5 cut(s)	331, 451, 466, 853, 958
Bse3DI	GCAATG	1 cut(s)	709
Bse8I	GATNNNNATC	1 cut(s)	753
BseBI	CCWGG	3 cut(s)	152, 224, 555
BseDI	CCNNGG	3 cut(s)	60, 546, 598
BseGI	GGATG	6 cut(s)	74, 265, 872, 1123, 1197, 1438
BseJI	GATNNNNATC	1 cut(s)	753
BseLI	CCNNNNNNNGG	1 cut(s)	1448
BseMI	GCAATG	1 cut(s)	709
BseMII	CTCAG	5 cut(s)	278, 314, 806, 1067, 1356
BseNI	ACTGG	5 cut(s)	331, 451, 466, 853, 958
BseRI	GAGGAG	3 cut(s)	285, 288, 1434
BseXI	GCAGC	5 cut(s)	127, 455, 884, 1251, 1525
BsgI	GTGCAG	1 cut(s)	891
Bsh1236I	CGCG	1 cut(s)	29
BshFI	GGCC	3 cut(s)	155, 597, 909
BshNI	GGYRCC	1 cut(s)	346
BsiHKAI	GWGCWC	2 cut(s)	774, 1380
BsiSI	CCGG	3 cut(s)	133, 861, 1261
BslFI	GGGAC	1 cut(s)	1093
BslI	CCNNNNNNNGG	1 cut(s)	1448
BsmAI	GTCTC	1 cut(s)	246
BsmFI	GGGAC	1 cut(s)	1093
BsmI	GAATGC	1 cut(s)	977
BsnI	GGCC	3 cut(s)	155, 597, 909
Bsp1286I	GDGCHC	2 cut(s)	774, 1380
Bsp143I	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
BspACI	CCGC	2 cut(s)	27, 700
BspANI	GGCC	3 cut(s)	155, 597, 909
BspCNI	CTCAG	5 cut(s)	277, 313, 805, 1066, 1357
BspFNI	CGCG	1 cut(s)	29
BspLI	GGNNCC	5 cut(s)	208, 348, 552, 750, 949
BspMAI	CTGCAG	1 cut(s)	445
BspPI	GGATC	3 cut(s)	743, 756, 1233
BspT107I	GGYRCC	1 cut(s)	346
BspTI	CTTAAG	1 cut(s)	14
BsrDI	GCAATG	1 cut(s)	709
BsrFI	RCCGGY	2 cut(s)	860, 1260
BsrI	ACTGG	5 cut(s)	331, 451, 466, 853, 958
BssAI	RCCGGY	2 cut(s)	860, 1260
BssECI	CCNNGG	3 cut(s)	60, 546, 598
BssMI	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
BssSI	CACGAG	2 cut(s)	1083, 1446
BssT1I	CCWWGG	3 cut(s)	60, 546, 598
Bst2BI	CACGAG	2 cut(s)	1083, 1446
Bst2UI	CCWGG	3 cut(s)	152, 224, 555
Bst4CI	ACNGT	6 cut(s)	313, 502, 839, 982, 1183, 1498
BstAFI	CTTAAG	1 cut(s)	14
BstAPI	GCANNNNNTGC	3 cut(s)	878, 1375, 1538
BstC8I	GCNNGC	3 cut(s)	379, 441, 1144
BstDEI	CTNAG	5 cut(s)	264, 300, 792, 1053, 1365
BstF5I	GGATG	6 cut(s)	74, 265, 872, 1123, 1197, 1438
BstFNI	CGCG	1 cut(s)	29
BstKTI	GATC	9 cut(s)	124, 318, 511, 526, 751, 834, 1047, 1228, 1357
BstMAI	GTCTC	1 cut(s)	246
BstMBI	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
BstMWI	GCNNNNNNNGC	9 cut(s)	176, 414, 423, 594, 878, 947, 1201, 1375, 1538
BstNI	CCWGG	3 cut(s)	152, 224, 555
BstSCI	CCNGG	4 cut(s)	132, 150, 222, 553
BstSFI	CTRYAG	2 cut(s)	421, 441
BstUI	CGCG	1 cut(s)	29
BstV1I	GCAGC	5 cut(s)	127, 455, 884, 1251, 1525
BstV2I	GAAGAC	2 cut(s)	495, 1302
BstX2I	RGATCY	2 cut(s)	748, 1044
BstXI	CCANNNNNNTGG	1 cut(s)	944
BstYI	RGATCY	2 cut(s)	748, 1044
BsuRI	GGCC	3 cut(s)	155, 597, 909
BtsCI	GGATG	6 cut(s)	74, 265, 872, 1123, 1197, 1438
BtsIMutI	CAGTG	5 cut(s)	309, 324, 951, 1420, 1503
Cac8I	GCNNGC	3 cut(s)	379, 441, 1144
CaiI	CAGNNNCTG	1 cut(s)	1538
Cfr10I	RCCGGY	2 cut(s)	860, 1260
Cfr13I	GGNCC	3 cut(s)	908, 1030, 1451
CseI	GACGC	1 cut(s)	40
Csp6I	GTAC	5 cut(s)	268, 291, 397, 1436, 1489
CspCI	CAANNNNNGTGG	2 cut(s)	940, 975
CviAII	CATG	7 cut(s)	256, 494, 649, 817, 938, 1315, 1480
CviQI	GTAC	5 cut(s)	268, 291, 397, 1436, 1489
DdeI	CTNAG	5 cut(s)	264, 300, 792, 1053, 1365
DpnI	GATC	9 cut(s)	123, 317, 510, 525, 750, 833, 1046, 1227, 1356
DpnII	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
DraIII	CACNNNGTG	1 cut(s)	1498
Eco130I	CCWWGG	3 cut(s)	60, 546, 598
Eco47I	GGWCC	2 cut(s)	1030, 1451
EcoRII	CCWGG	3 cut(s)	150, 222, 553
EcoT14I	CCWWGG	3 cut(s)	60, 546, 598
EcoT22I	ATGCAT	1 cut(s)	1412
ErhI	CCWWGG	3 cut(s)	60, 546, 598
FaeI	CATG	7 cut(s)	259, 497, 652, 820, 941, 1318, 1483
FalI	AAGNNNNNCTT	1 cut(s)	29
FaqI	GGGAC	1 cut(s)	1093
FatI	CATG	7 cut(s)	255, 493, 648, 816, 937, 1314, 1479
FauNDI	CATATG	1 cut(s)	778
FbaI	TGATCA	1 cut(s)	121
Fnu4HI	GCNGC	5 cut(s)	141, 444, 873, 1265, 1539
FokI	GGATG	6 cut(s)	61, 272, 879, 1130, 1204, 1445
Fsp4HI	GCNGC	5 cut(s)	141, 444, 873, 1265, 1539
FspBI	CTAG	3 cut(s)	1269, 1320, 1358
GluI	GCNGC	5 cut(s)	141, 444, 873, 1265, 1539
GsuI	CTGGAG	1 cut(s)	134
HaeIII	GGCC	3 cut(s)	155, 597, 909
HapII	CCGG	3 cut(s)	133, 861, 1261
HgaI	GACGC	1 cut(s)	40
Hin1II	CATG	7 cut(s)	259, 497, 652, 820, 941, 1318, 1483
HinfI	GANTC	8 cut(s)	884, 964, 1081, 1101, 1213, 1246, 1283, 1462
HpaII	CCGG	3 cut(s)	133, 861, 1261
HphI	GGTGA	4 cut(s)	518, 737, 833, 1406
Hpy166II	GTNNAC	3 cut(s)	309, 728, 1451
Hpy188I	TCNGA	6 cut(s)	126, 303, 767, 795, 1056, 1153
Hpy188III	TCNNGA	8 cut(s)	247, 370, 527, 608, 888, 968, 1105, 1459
Hpy8I	GTNNAC	3 cut(s)	309, 728, 1451
Hpy99I	CGWCG	2 cut(s)	34, 839
HpyAV	CCTTC	4 cut(s)	360, 713, 790, 1339
HpyCH4III	ACNGT	6 cut(s)	313, 502, 839, 982, 1183, 1498
HpyF10VI	GCNNNNNNNGC	9 cut(s)	176, 414, 423, 594, 878, 947, 1201, 1375, 1538
HpyF3I	CTNAG	5 cut(s)	264, 300, 792, 1053, 1365
Hsp92II	CATG	7 cut(s)	259, 497, 652, 820, 941, 1318, 1483
Ksp22I	TGATCA	1 cut(s)	121
Kzo9I	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
LmnI	GCTCC	3 cut(s)	96, 550, 947
Lsp1109I	GCAGC	5 cut(s)	127, 455, 884, 1251, 1525
LweI	GCATC	7 cut(s)	83, 157, 250, 567, 1144, 1182, 1419
MaeI	CTAG	3 cut(s)	1269, 1320, 1358
MaeIII	GTNAC	4 cut(s)	22, 496, 787, 1256
MalI	GATC	9 cut(s)	123, 317, 510, 525, 750, 833, 1046, 1227, 1356
MboI	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
MboII	GAAGA	8 cut(s)	124, 191, 345, 500, 725, 728, 1054, 1302
MfeI	CAATTG	2 cut(s)	876, 1335
MflI	RGATCY	2 cut(s)	748, 1044
MhlI	GDGCHC	2 cut(s)	774, 1380
MlyI	GAGTC	2 cut(s)	973, 1075
MmeI	TCCRAC	4 cut(s)	79, 601, 745, 1078
MnlI	CCTC	9 cut(s)	59, 306, 309, 532, 716, 1384, 1390, 1452, 1455
Mph1103I	ATGCAT	1 cut(s)	1412
MseI	TTAA	4 cut(s)	15, 654, 669, 696
MslI	CAYNNNNRTG	1 cut(s)	777
MspCI	CTTAAG	1 cut(s)	14
MspI	CCGG	3 cut(s)	133, 861, 1261
MspR9I	CCNGG	4 cut(s)	134, 152, 224, 555
MunI	CAATTG	2 cut(s)	876, 1335
Mva1269I	GAATGC	1 cut(s)	977
MvaI	CCWGG	3 cut(s)	152, 224, 555
MvnI	CGCG	1 cut(s)	29
MwoI	GCNNNNNNNGC	9 cut(s)	176, 414, 423, 594, 878, 947, 1201, 1375, 1538
NciI	CCSGG	1 cut(s)	134
NdeI	CATATG	1 cut(s)	778
NdeII	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
NlaIII	CATG	7 cut(s)	259, 497, 652, 820, 941, 1318, 1483
NlaIV	GGNNCC	5 cut(s)	208, 348, 552, 750, 949
NmuCI	GTSAC	1 cut(s)	1256
NsiI	ATGCAT	1 cut(s)	1412
PctI	GAATGC	1 cut(s)	977
PfeI	GAWTC	6 cut(s)	884, 1101, 1213, 1246, 1283, 1462
PkrI	GCNGC	5 cut(s)	142, 445, 874, 1266, 1540
PleI	GAGTC	2 cut(s)	972, 1075
PpsI	GAGTC	2 cut(s)	972, 1075
Psp6I	CCWGG	3 cut(s)	150, 222, 553
PspGI	CCWGG	3 cut(s)	150, 222, 553
PspN4I	GGNNCC	5 cut(s)	208, 348, 552, 750, 949
PspPI	GGNCC	3 cut(s)	908, 1030, 1451
PstI	CTGCAG	1 cut(s)	445
PstNI	CAGNNNCTG	1 cut(s)	1538
PsuI	RGATCY	2 cut(s)	748, 1044
RsaI	GTAC	5 cut(s)	269, 292, 398, 1437, 1490
RsaNI	GTAC	5 cut(s)	268, 291, 397, 1436, 1489
RseI	CAYNNNNRTG	1 cut(s)	777
SaqAI	TTAA	4 cut(s)	15, 654, 669, 696
SatI	GCNGC	5 cut(s)	141, 444, 873, 1265, 1539
Sau3AI	GATC	9 cut(s)	121, 315, 508, 523, 748, 831, 1044, 1225, 1354
Sau96I	GGNCC	3 cut(s)	908, 1030, 1451
ScaI	AGTACT	1 cut(s)	269
SchI	GAGTC	2 cut(s)	973, 1075
ScrFI	CCNGG	4 cut(s)	134, 152, 224, 555
SduI	GDGCHC	2 cut(s)	774, 1380
SfaNI	GCATC	7 cut(s)	83, 157, 250, 567, 1144, 1182, 1419
SfcI	CTRYAG	2 cut(s)	421, 441
SinI	GGWCC	2 cut(s)	1030, 1451
SmiMI	CAYNNNNRTG	1 cut(s)	777
SmlI	CTYRAG	3 cut(s)	14, 245, 1205
SmoI	CTYRAG	3 cut(s)	14, 245, 1205
SsiI	CCGC	2 cut(s)	27, 700
SspMI	CTAG	3 cut(s)	1269, 1320, 1358
StyD4I	CCNGG	4 cut(s)	132, 150, 222, 553
StyI	CCWWGG	3 cut(s)	60, 546, 598
TaaI	ACNGT	6 cut(s)	313, 502, 839, 982, 1183, 1498
TaqI	TCGA	3 cut(s)	371, 537, 834
TatI	WGTACW	2 cut(s)	267, 396
TfiI	GAWTC	6 cut(s)	884, 1101, 1213, 1246, 1283, 1462
Tru1I	TTAA	4 cut(s)	15, 654, 669, 696
Tru9I	TTAA	4 cut(s)	15, 654, 669, 696
TscAI	CASTG	5 cut(s)	316, 331, 958, 1420, 1503
TseFI	GTSAC	1 cut(s)	1256
TseI	GCWGC	5 cut(s)	140, 443, 872, 1264, 1538
Tsp45I	GTSAC	1 cut(s)	1256
TspDTI	ATGAA	1 cut(s)	1468
TspGWI	ACGGA	1 cut(s)	83
TspRI	CASTG	5 cut(s)	316, 331, 958, 1420, 1503
Vha464I	CTTAAG	1 cut(s)	14
VpaK11BI	GGWCC	2 cut(s)	1030, 1451
XapI	RAATTY	4 cut(s)	337, 530, 578, 612
XcmI	CCANNNNNNNNNTGG	3 cut(s)	158, 458, 500
XspI	CTAG	3 cut(s)	1269, 1320, 1358
ZrmI	AGTACT	1 cut(s)	269
Zsp2I	ATGCAT	1 cut(s)	1412

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.