Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0001862

Physical Location & Seq

Genomic Coordinates Reverse (-)

35181228 .. 35186948

Gene Structure 5721 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1159300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1566 bp

ATGGGTTTCTTCATGCAGTTGAATATGGAAACCAAACTCAAGTCTGTAACAGCGACGCTCTTTCTGTGCTTCCTCGTGTTTCCGTTGGTTTTTTGCGAGTCCAACGATGGGTTGCTCCGGATTGGGCTCAAAAAGAGGAAATTGGACCAAAATAACATGGTTGCTTCCCAGATTGATTCCAAGGAAGGAGAGTCTCTGAGAGCTACGCTCCAAAAGTATCGTCTGGGTGGAAACTTGGCTGATTCTCAGGACATTGATATTGTATCTCTCAAAAATTATATGGATGCTCAATACTTTGGTGAGATCGGTATTGGCACTCCCCCTCAGAAGTTCACTGTGATATTTGATACTGGTAGTGCAAATTTGTGGGTGCCTTCGTCTAAGTGTTTTTTCTCGATTGCATGCTATTTACATCCAAAATACAAGTCAAGCAGCTCCAGCACCTACAACAAAAATGGTAAGCCTGCAGCCATCCAGTATGGAACTGGAGCTATTTCTGGGTTCTTCAGTGAAGATCATGTAACAGTTGGTGATCTTGTGGTTAAAGATCAGGAATTTATCGAGGCAACCAAGGAACCCGGCGTCACATTTGTGGTAGCCAAATTTGATGGCATACTTGGACTTGGATTTCAAGAGATTTCAGTTGGAAATGCTGTACCTGTGTGGTATAACATGGTCAATCAGGGTCTTCTGAAGGAACCAGTTTTCTCATTCTGGTTTAATCGTAATGCTGACGAAGAAGAAGGAGGTGAAATTGTTTTTGGTGGAGTGGATCCCAATCACTACAAGGGAGAGCACACATATGTTCCTGTTACACAGAAAGGCTATTGGCAGTTTGACATGGGTGATGTTATGATTGATGGTCAAACTACTGGATTTTGTGCTAGTGGCTGTTCGGCTATTGCTGATTCTGGAACTTCTTTGTTGGTTGGCCCAACGACTATTATTACTGAACTCAATCATGCCATTGGAGCCTCTGGCATTGTAAGTCAAGAATGCAAGACTGTAGTTGCAGAATATGGGGAAACCATAATTGAGAGAATTTTAGCAAAGGATCAACCAGCAAAAATCTGCTCTCAGATTGGTTTGTGCTCATTTGATGGGACTCATGGTGTTAGTATCGGAATCAAGAGTGTTGTGGAGAACACTCACAAGGTATCTGGTGACTTATCTGATGCAACGTGTTCTGCTTGTGAGATGACGGTTGTTTGGATGCAAAACCAGCTCAAGCAGAATCAGACACAGGAGCGAATTCTTGACTATGTGAATCAGCTCTGTGATCGGTTGCCTAGCCCAATGGGAGAATCTGCAGTTGATTGTGCTGGTTTGTCATCAATGCCCAGTGTTTCCTTCACTATCGGTGGAAGAACATTTGATCTCACCCCTGAACAGTACGTGCTCAAAGTTGGTGAGGGAGATGCAGCACAATGCATTAGTGGATTTACTGCCTTGGATGTCCCACCTCCCCGCGGACCTCTCTGGATCTTGGGTGATGTATTCATGGGTCGCTACCACACCGTATTTGACTATGGCAATGAGAGGATTGGGTTTGCAGAAGCTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

522

Amino Acids

56.68

Weight (kDa)

4.84

Isoelectric Point (pI)

32.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	97 - 520	4.5e-119	Eukaryotic aspartyl protease
TAXi_N	PF14543	98 - 255	1.8e-10	Xylanase inhibitor N-terminal
SapB_2	PF03489	331 - 364	1.3e-10	Saposin-like type B, region 2
SapB_1	PF05184	394 - 430	4.4e-14	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11910 AT1G11910 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290
fragaria_vesca	FvH4_3g23140 FvH4_3g23140 FvH4_6g42880 FvH4_6g42880
malus_domestica	MD00G1159300.v1.1 MD09G1111100.v1.1 MD11G1213600.v1.1 MD15G1413400.v1.1 MD17G1098900.v1.1 MD17G1282600.v1.1 MD17G1282700.v1.1
prunus_persica	Prupe.3G214900_v2.0.a1 Prupe.3G214900_v2.0.a1 Prupe.4G217700_v2.0.a1
pyrus_communis	pycom03g14920 pycom09g03360 pycom11g18820 pycom17g09430
rosa_chinensis	RchiOBHm_Chr2g0158811 RchiOBHm_Chr5g0040781
rosa_laevigata	RLG00000021115 RLG00000034001
rosa_multiflora	Rmu_sc0001579.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039910 Rroxscaffold_2G00090980
rosa_rugosa	Rorug02G0472700 Rorug05G0187200 Rorug05G0187300
rosa_samantha	Rh2AG538500 Rh2BG551200 Rh2CG521800 Rh2DG561000 Rh5AG273000 Rh5BG278100 Rh5CG310100 Rh5DG286000
rosa_wichuraiana	Rw2G044570 Rw5G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	370
AccII	CGCG	1 cut(s)	1470
AccIII	TCCGGA	1 cut(s)	117
AciI	CCGC	2 cut(s)	1468, 1470
AclWI	GGATC	4 cut(s)	767, 780, 1062, 1490
AcsI	RAATTY	5 cut(s)	361, 554, 602, 1041, 1251
AcuI	CTGAAG	2 cut(s)	490, 713
AcyI	GRCGYC	1 cut(s)	582
AfaI	GTAC	2 cut(s)	657, 1394
AfiI	CCNNNNNNNGG	2 cut(s)	108, 1469
AflIII	ACRYGT	1 cut(s)	1181
AgsI	TTSAA	2 cut(s)	22, 632
AjuI	GAANNNNNNNTTGG	2 cut(s)	744, 776
AleI	CACNNNNGTG	1 cut(s)	590
AluBI	AGCT	6 cut(s)	203, 435, 491, 1225, 1273, 1559
AluI	AGCT	6 cut(s)	203, 435, 491, 1225, 1273, 1559
Alw21I	GWGCWC	3 cut(s)	798, 1094, 1401
Alw26I	GTCTC	1 cut(s)	198
AlwI	GGATC	4 cut(s)	767, 780, 1062, 1490
AlwNI	CAGNNNCTG	1 cut(s)	1559
Aor13HI	TCCGGA	1 cut(s)	117
AoxI	GGCC	1 cut(s)	931
ApeKI	GCWGC	4 cut(s)	432, 467, 1421, 1559
ApoI	RAATTY	5 cut(s)	361, 554, 602, 1041, 1251
ArsI	GACNNNNNNTTYG	4 cut(s)	205, 237, 410, 442
AspS9I	GGNCC	3 cut(s)	145, 932, 1472
AsuC2I	CCSGG	1 cut(s)	579
AsuHPI	GGTGA	8 cut(s)	311, 542, 761, 857, 1175, 1372, 1421, 1502
AvaII	GGWCC	2 cut(s)	145, 1472
BamHI	GGATCC	1 cut(s)	772
BanI	GGYRCC	1 cut(s)	370
BanII	GRGCYC	1 cut(s)	129
BauI	CACGAG	1 cut(s)	74
BbsI	GAAGAC	1 cut(s)	680
Bbv12I	GWGCWC	3 cut(s)	798, 1094, 1401
BbvI	GCAGC	4 cut(s)	444, 479, 1433, 1546
BccI	CCATC	5 cut(s)	101, 479, 602, 854, 1094
BcnI	CCSGG	1 cut(s)	579
BcoDI	GTCTC	1 cut(s)	198
BfaI	CTAG	2 cut(s)	885, 1290
BfmI	CTRYAG	3 cut(s)	465, 1005, 1308
BisI	GCNGC	4 cut(s)	433, 468, 1422, 1560
BlsI	GCNGC	4 cut(s)	434, 469, 1423, 1561
Bme1390I	CCNGG	1 cut(s)	579
Bme18I	GGWCC	2 cut(s)	145, 1472
BmgT120I	GGNCC	3 cut(s)	145, 932, 1472
BmiI	GGNNCC	5 cut(s)	372, 576, 699, 774, 973
BmrFI	CCNGG	1 cut(s)	579
BmrI	ACTGGG	1 cut(s)	1335
BmsI	GCATC	4 cut(s)	274, 1165, 1203, 1408
BmuI	ACTGGG	1 cut(s)	1335
BpiI	GAAGAC	1 cut(s)	680
BplI	GAGNNNNNCTC	2 cut(s)	192, 224
BpmI	CTGGAG	2 cut(s)	421, 507
BpuEI	CTTGAG	2 cut(s)	23, 1211
BpuMI	CCSGG	1 cut(s)	579
BsaAI	YACGTR	1 cut(s)	1396
BsaBI	GATNNNNATC	1 cut(s)	777
BsaHI	GRCGYC	1 cut(s)	582
BsaJI	CCNNGG	4 cut(s)	180, 570, 1449, 1468
BsaWI	WCCGGW	1 cut(s)	117
Bsc4I	CCNNNNNNNGG	2 cut(s)	108, 1469
Bse1I	ACTGG	6 cut(s)	355, 475, 490, 701, 877, 1341
Bse3DI	GCAATG	1 cut(s)	1540
Bse8I	GATNNNNATC	1 cut(s)	777
BseAI	TCCGGA	1 cut(s)	117
BseDI	CCNNGG	4 cut(s)	180, 570, 1449, 1468
BseGI	GGATG	5 cut(s)	289, 412, 471, 1218, 1459
BseJI	GATNNNNATC	1 cut(s)	777
BseLI	CCNNNNNNNGG	2 cut(s)	108, 1469
BseMI	GCAATG	1 cut(s)	1540
BseMII	CTCAG	4 cut(s)	188, 260, 338, 1091
BseNI	ACTGG	6 cut(s)	355, 475, 490, 701, 877, 1341
BseXI	GCAGC	4 cut(s)	444, 479, 1433, 1546
Bsh1236I	CGCG	1 cut(s)	1470
BshFI	GGCC	1 cut(s)	933
BshNI	GGYRCC	1 cut(s)	370
BsiHKAI	GWGCWC	3 cut(s)	798, 1094, 1401
BsiSI	CCGG	2 cut(s)	118, 579
BslFI	GGGAC	2 cut(s)	1117, 1442
BslI	CCNNNNNNNGG	2 cut(s)	108, 1469
BsmAI	GTCTC	1 cut(s)	198
BsmFI	GGGAC	2 cut(s)	1117, 1442
BsmI	GAATGC	1 cut(s)	1001
BsnI	GGCC	1 cut(s)	933
Bsp1286I	GDGCHC	4 cut(s)	129, 798, 1094, 1401
Bsp13I	TCCGGA	1 cut(s)	117
Bsp143I	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
BspACI	CCGC	2 cut(s)	1468, 1470
BspANI	GGCC	1 cut(s)	933
BspCNI	CTCAG	4 cut(s)	189, 259, 337, 1090
BspEI	TCCGGA	1 cut(s)	117
BspFNI	CGCG	1 cut(s)	1470
BspLI	GGNNCC	5 cut(s)	372, 576, 699, 774, 973
BspMAI	CTGCAG	2 cut(s)	469, 1312
BspPI	GGATC	4 cut(s)	767, 780, 1062, 1490
BspT107I	GGYRCC	1 cut(s)	370
BsrDI	GCAATG	1 cut(s)	1540
BsrI	ACTGG	6 cut(s)	355, 475, 490, 701, 877, 1341
BssECI	CCNNGG	4 cut(s)	180, 570, 1449, 1468
BssMI	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
BssNI	GRCGYC	1 cut(s)	582
BssSI	CACGAG	1 cut(s)	74
BssT1I	CCWWGG	3 cut(s)	180, 570, 1449
Bst2BI	CACGAG	1 cut(s)	74
Bst4CI	ACNGT	6 cut(s)	337, 526, 1006, 1204, 1392, 1519
BstACI	GRCGYC	1 cut(s)	582
BstAPI	GCANNNNNTGC	1 cut(s)	1559
BstBAI	YACGTR	1 cut(s)	1396
BstC8I	GCNNGC	2 cut(s)	403, 465
BstDEI	CTNAG	5 cut(s)	197, 246, 324, 381, 1077
BstDSI	CCRYGG	1 cut(s)	1468
BstF5I	GGATG	5 cut(s)	289, 412, 471, 1218, 1459
BstFNI	CGCG	1 cut(s)	1470
BstKTI	GATC	9 cut(s)	306, 517, 535, 550, 775, 1057, 1282, 1378, 1485
BstMAI	GTCTC	1 cut(s)	198
BstMBI	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
BstMWI	GCNNNNNNNGC	4 cut(s)	438, 971, 1222, 1559
BstNSI	RCATGY	1 cut(s)	405
BstSCI	CCNGG	1 cut(s)	577
BstSFI	CTRYAG	3 cut(s)	465, 1005, 1308
BstUI	CGCG	1 cut(s)	1470
BstV1I	GCAGC	4 cut(s)	444, 479, 1433, 1546
BstV2I	GAAGAC	1 cut(s)	680
BstX2I	RGATCY	2 cut(s)	772, 1482
BstYI	RGATCY	2 cut(s)	772, 1482
BsuRI	GGCC	1 cut(s)	933
BtgI	CCRYGG	1 cut(s)	1468
BtsCI	GGATG	5 cut(s)	289, 412, 471, 1218, 1459
BtsIMutI	CAGTG	3 cut(s)	333, 514, 1348
Cac8I	GCNNGC	2 cut(s)	403, 465
CaiI	CAGNNNCTG	1 cut(s)	1559
Cfr13I	GGNCC	3 cut(s)	145, 932, 1472
Cfr42I	CCGCGG	1 cut(s)	1471
CseI	GACGC	2 cut(s)	64, 571
Csp6I	GTAC	2 cut(s)	656, 1393
CviAII	CATG	9 cut(s)	13, 157, 402, 518, 673, 841, 962, 1109, 1501
CviQI	GTAC	2 cut(s)	656, 1393
DdeI	CTNAG	5 cut(s)	197, 246, 324, 381, 1077
DpnI	GATC	9 cut(s)	305, 516, 534, 549, 774, 1056, 1281, 1377, 1484
DpnII	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
Eco130I	CCWWGG	3 cut(s)	180, 570, 1449
Eco24I	GRGCYC	1 cut(s)	129
Eco47I	GGWCC	2 cut(s)	145, 1472
Eco57I	CTGAAG	2 cut(s)	490, 713
EcoRI	GAATTC	1 cut(s)	1251
EcoT14I	CCWWGG	3 cut(s)	180, 570, 1449
EcoT22I	ATGCAT	1 cut(s)	1433
EcoT38I	GRGCYC	1 cut(s)	129
ErhI	CCWWGG	3 cut(s)	180, 570, 1449
FaeI	CATG	9 cut(s)	16, 160, 405, 521, 676, 844, 965, 1112, 1504
FaqI	GGGAC	2 cut(s)	1117, 1442
FatI	CATG	9 cut(s)	12, 156, 401, 517, 672, 840, 961, 1108, 1500
FauI	CCCGC	1 cut(s)	1475
FauNDI	CATATG	1 cut(s)	802
Fnu4HI	GCNGC	4 cut(s)	433, 468, 1422, 1560
FokI	GGATG	5 cut(s)	296, 399, 458, 1225, 1466
FriOI	GRGCYC	1 cut(s)	129
Fsp4HI	GCNGC	4 cut(s)	433, 468, 1422, 1560
FspBI	CTAG	2 cut(s)	885, 1290
GluI	GCNGC	4 cut(s)	433, 468, 1422, 1560
GsuI	CTGGAG	2 cut(s)	421, 507
HaeIII	GGCC	1 cut(s)	933
HapII	CCGG	2 cut(s)	118, 579
HgaI	GACGC	2 cut(s)	64, 571
Hin1I	GRCGYC	1 cut(s)	582
Hin1II	CATG	9 cut(s)	16, 160, 405, 521, 676, 844, 965, 1112, 1504
HpaII	CCGG	2 cut(s)	118, 579
HphI	GGTGA	8 cut(s)	311, 542, 761, 857, 1175, 1372, 1421, 1502
Hpy166II	GTNNAC	1 cut(s)	333
Hpy188I	TCNGA	7 cut(s)	198, 327, 693, 1080, 1124, 1174, 1239
Hpy8I	GTNNAC	1 cut(s)	333
Hpy99I	CGWCG	1 cut(s)	58
HpyAV	CCTTC	5 cut(s)	179, 384, 688, 737, 1360
HpyCH4III	ACNGT	6 cut(s)	337, 526, 1006, 1204, 1392, 1519
HpyCH4IV	ACGT	2 cut(s)	1181, 1395
HpyF10VI	GCNNNNNNNGC	4 cut(s)	438, 971, 1222, 1559
HpyF3I	CTNAG	5 cut(s)	197, 246, 324, 381, 1077
HpySE526I	ACGT	2 cut(s)	1181, 1395
Hsp92I	GRCGYC	1 cut(s)	582
Hsp92II	CATG	9 cut(s)	16, 160, 405, 521, 676, 844, 965, 1112, 1504
Kpn2I	TCCGGA	1 cut(s)	117
KspI	CCGCGG	1 cut(s)	1471
Kzo9I	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
LmnI	GCTCC	6 cut(s)	120, 213, 440, 488, 971, 1246
Lsp1109I	GCAGC	4 cut(s)	444, 479, 1433, 1546
LweI	GCATC	4 cut(s)	274, 1165, 1203, 1408
MaeI	CTAG	2 cut(s)	885, 1290
MaeII	ACGT	2 cut(s)	1181, 1395
MaeIII	GTNAC	5 cut(s)	46, 520, 583, 811, 1163
MalI	GATC	9 cut(s)	305, 516, 534, 549, 774, 1056, 1281, 1377, 1484
MboI	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
MboII	GAAGA	6 cut(s)	496, 524, 680, 749, 752, 1377
MflI	RGATCY	2 cut(s)	772, 1482
MhlI	GDGCHC	4 cut(s)	129, 798, 1094, 1401
MluCI	AATT	9 cut(s)	140, 274, 361, 554, 602, 753, 1032, 1041, 1251
MlyI	GAGTC	3 cut(s)	107, 200, 1099
MmeI	TCCRAC	2 cut(s)	126, 625
Mph1103I	ATGCAT	1 cut(s)	1433
MroI	TCCGGA	1 cut(s)	117
MseI	TTAA	2 cut(s)	543, 720
MslI	CAYNNNNRTG	2 cut(s)	590, 801
MspA1I	CMGCKG	1 cut(s)	1470
MspI	CCGG	2 cut(s)	118, 579
MspR9I	CCNGG	1 cut(s)	579
Mva1269I	GAATGC	1 cut(s)	1001
MvnI	CGCG	1 cut(s)	1470
MwoI	GCNNNNNNNGC	4 cut(s)	438, 971, 1222, 1559
NciI	CCSGG	1 cut(s)	579
NdeI	CATATG	1 cut(s)	802
NdeII	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
NlaIII	CATG	9 cut(s)	16, 160, 405, 521, 676, 844, 965, 1112, 1504
NlaIV	GGNNCC	5 cut(s)	372, 576, 699, 774, 973
NmuCI	GTSAC	2 cut(s)	583, 1163
NsiI	ATGCAT	1 cut(s)	1433
NspI	RCATGY	1 cut(s)	405
OliI	CACNNNNGTG	1 cut(s)	590
PaeI	GCATGC	1 cut(s)	405
PctI	GAATGC	1 cut(s)	1001
PfeI	GAWTC	7 cut(s)	176, 242, 908, 1125, 1234, 1267, 1304
PkrI	GCNGC	4 cut(s)	434, 469, 1423, 1561
PleI	GAGTC	3 cut(s)	106, 199, 1099
PpsI	GAGTC	3 cut(s)	106, 199, 1099
Ppu21I	YACGTR	1 cut(s)	1396
PspN4I	GGNNCC	5 cut(s)	372, 576, 699, 774, 973
PspPI	GGNCC	3 cut(s)	145, 932, 1472
PstI	CTGCAG	2 cut(s)	469, 1312
PstNI	CAGNNNCTG	1 cut(s)	1559
PsuI	RGATCY	2 cut(s)	772, 1482
RsaI	GTAC	2 cut(s)	657, 1394
RsaNI	GTAC	2 cut(s)	656, 1393
RseI	CAYNNNNRTG	2 cut(s)	590, 801
SacII	CCGCGG	1 cut(s)	1471
SaqAI	TTAA	2 cut(s)	543, 720
SatI	GCNGC	4 cut(s)	433, 468, 1422, 1560
Sau3AI	GATC	9 cut(s)	303, 514, 532, 547, 772, 1054, 1279, 1375, 1482
Sau96I	GGNCC	3 cut(s)	145, 932, 1472
SchI	GAGTC	3 cut(s)	107, 200, 1099
ScrFI	CCNGG	1 cut(s)	579
SduI	GDGCHC	4 cut(s)	129, 798, 1094, 1401
SfaNI	GCATC	4 cut(s)	274, 1165, 1203, 1408
SfcI	CTRYAG	3 cut(s)	465, 1005, 1308
Sfr303I	CCGCGG	1 cut(s)	1471
SgrBI	CCGCGG	1 cut(s)	1471
SinI	GGWCC	2 cut(s)	145, 1472
SmiMI	CAYNNNNRTG	2 cut(s)	590, 801
SmlI	CTYRAG	2 cut(s)	38, 1226
SmoI	CTYRAG	2 cut(s)	38, 1226
SphI	GCATGC	1 cut(s)	405
Sse9I	AATT	9 cut(s)	140, 274, 361, 554, 602, 753, 1032, 1041, 1251
SsiI	CCGC	2 cut(s)	1468, 1470
SspMI	CTAG	2 cut(s)	885, 1290
StyD4I	CCNGG	1 cut(s)	577
StyI	CCWWGG	3 cut(s)	180, 570, 1449
TaaI	ACNGT	6 cut(s)	337, 526, 1006, 1204, 1392, 1519
TaiI	ACGT	2 cut(s)	1184, 1398
TaqI	TCGA	2 cut(s)	395, 561
TasI	AATT	9 cut(s)	140, 274, 361, 554, 602, 753, 1032, 1041, 1251
TfiI	GAWTC	7 cut(s)	176, 242, 908, 1125, 1234, 1267, 1304
Tru1I	TTAA	2 cut(s)	543, 720
Tru9I	TTAA	2 cut(s)	543, 720
TscAI	CASTG	3 cut(s)	340, 514, 1348
TseFI	GTSAC	2 cut(s)	583, 1163
TseI	GCWGC	4 cut(s)	432, 467, 1421, 1559
Tsp45I	GTSAC	2 cut(s)	583, 1163
TspDTI	ATGAA	1 cut(s)	1489
TspGWI	ACGGA	1 cut(s)	72
TspRI	CASTG	3 cut(s)	340, 514, 1348
VpaK11BI	GGWCC	2 cut(s)	145, 1472
XapI	RAATTY	5 cut(s)	361, 554, 602, 1041, 1251
XceI	RCATGY	1 cut(s)	405
XcmI	CCANNNNNNNNNTGG	1 cut(s)	482
XspI	CTAG	2 cut(s)	885, 1290
Zsp2I	ATGCAT	1 cut(s)	1433

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.