MD17G1282700.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001862
Physical Location & Seq
Forward (+)
34171261 .. 34171787
527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1282700.v1.1.491

Sequence Viewer

Length: 447 bp
ATGAAAAAGGAGATTCAATGTGCGGCGATCCAGTATGGAACTGGTGCAATTTCAGGTTTCTTCCTCCACCAGGACCATGTCCAAGTCGGTGATCTTGTTGTTAAGGATCAGGACTTTATTGAGGCAACCAAAGAGCCTGGAGTCACATTTGTGGCAGCTAAGTTTGATGGTATTCTTGGACTTGGATTTCAAGATATCTCAGTTGGAGATGCTGTTCCTGTCTGGTATAATTTGGTCAATCAAGGTCTTGTTAAAGAACCAGTTTTCTCATTTTGGTTAAATCGCAATACAGAAGGGGAAGAAAGGGGTGAGATTGTCTTTGGTGGGGTTGATTCTAGTCATTTCAAGGGTGAGCATACATATGTTCCCGTGACTCAGAAAGGCTACTGGCAGCACCTCCCTATTGGCTGGACCAACGGAAGTATGGAGAGGTGGTGGAGGCTGTGA

Protein Analysis

149

Amino Acids

16.65

Weight (kDa)

5.26

Isoelectric Point (pI)

25.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 5 - 131 3.1e-44 Eukaryotic aspartyl protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 23
AclWI GGATC 2 cut(s) 22, 114
AfiI CCNNNNNNNGG 1 cut(s) 70
AgsI TTSAA 3 cut(s) 17, 191, 346
AjnI CCWGG 2 cut(s) 69, 136
AleI CACNNNNGTG 1 cut(s) 149
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
AluBI AGCT 1 cut(s) 158
AluI AGCT 1 cut(s) 158
AlwI GGATC 2 cut(s) 22, 114
ApeKI GCWGC 2 cut(s) 155, 391
AspS9I GGNCC 2 cut(s) 73, 411
AsuHPI GGTGA 3 cut(s) 101, 320, 362
AvaII GGWCC 2 cut(s) 73, 411
BbvI GCAGC 2 cut(s) 167, 403
BccI CCATC 1 cut(s) 161
BciT130I CCWGG 2 cut(s) 71, 138
BfaI CTAG 1 cut(s) 336
BisI GCNGC 3 cut(s) 24, 156, 392
BlsI GCNGC 3 cut(s) 25, 157, 393
Bme1390I CCNGG 2 cut(s) 71, 138
Bme18I GGWCC 2 cut(s) 73, 411
BmgT120I GGNCC 2 cut(s) 73, 411
BmrFI CCNGG 2 cut(s) 71, 138
BmsI GCATC 1 cut(s) 199
BpmI CTGGAG 1 cut(s) 159
BsaXI ACNNNNNCTCC 3 cut(s) 198, 228, 419
Bsc4I CCNNNNNNNGG 1 cut(s) 70
Bse1I ACTGG 4 cut(s) 31, 46, 260, 392
BseBI CCWGG 2 cut(s) 71, 138
BseLI CCNNNNNNNGG 1 cut(s) 70
BseMII CTCAG 2 cut(s) 213, 389
BseNI ACTGG 4 cut(s) 31, 46, 260, 392
BseXI GCAGC 2 cut(s) 167, 403
BslI CCNNNNNNNGG 1 cut(s) 70
Bsp143I GATC 3 cut(s) 27, 91, 106
BspACI CCGC 1 cut(s) 23
BspCNI CTCAG 2 cut(s) 212, 388
BspPI GGATC 2 cut(s) 22, 114
BsrI ACTGG 4 cut(s) 31, 46, 260, 392
BssMI GATC 3 cut(s) 27, 91, 106
Bst2UI CCWGG 2 cut(s) 71, 138
BstDEI CTNAG 3 cut(s) 159, 199, 375
BstENI CCTNNNNNAGG 1 cut(s) 68
BstKTI GATC 3 cut(s) 30, 94, 109
BstMBI GATC 3 cut(s) 27, 91, 106
BstNI CCWGG 2 cut(s) 71, 138
BstSCI CCNGG 2 cut(s) 69, 136
BstV1I GCAGC 2 cut(s) 167, 403
Cfr13I GGNCC 2 cut(s) 73, 411
CviAII CATG 1 cut(s) 77
CviJI RGCY 5 cut(s) 136, 158, 384, 408, 442
CviKI_1 RGCY 5 cut(s) 136, 158, 384, 408, 442
DdeI CTNAG 3 cut(s) 159, 199, 375
DpnI GATC 3 cut(s) 29, 93, 108
DpnII GATC 3 cut(s) 27, 91, 106
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 2 cut(s) 73, 411
EcoNI CCTNNNNNAGG 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 69, 136
EcoRV GATATC 1 cut(s) 196
FaeI CATG 1 cut(s) 80
FaiI YATR 7 cut(s) 36, 78, 228, 357, 361, 363, 425
FatI CATG 1 cut(s) 76
FauNDI CATATG 1 cut(s) 361
Fnu4HI GCNGC 3 cut(s) 24, 156, 392
Fsp4HI GCNGC 3 cut(s) 24, 156, 392
FspBI CTAG 1 cut(s) 336
GluI GCNGC 3 cut(s) 24, 156, 392
GsuI CTGGAG 1 cut(s) 159
Hin1II CATG 1 cut(s) 80
HinfI GANTC 4 cut(s) 13, 141, 332, 373
HphI GGTGA 3 cut(s) 101, 320, 362
Hpy188I TCNGA 1 cut(s) 378
Hpy188III TCNNGA 2 cut(s) 110, 191
HpyAV CCTTC 1 cut(s) 287
HpyCH4V TGCA 1 cut(s) 47
HpyF3I CTNAG 3 cut(s) 159, 199, 375
Hsp92II CATG 1 cut(s) 80
Kzo9I GATC 3 cut(s) 27, 91, 106
Lsp1109I GCAGC 2 cut(s) 167, 403
LweI GCATC 1 cut(s) 199
MaeI CTAG 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 142, 370
MalI GATC 3 cut(s) 29, 93, 108
MboI GATC 3 cut(s) 27, 91, 106
MboII GAAGA 2 cut(s) 52, 311
MluCI AATT 2 cut(s) 48, 229
MlyI GAGTC 2 cut(s) 150, 367
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 5 cut(s) 74, 115, 407, 423, 432
MseI TTAA 3 cut(s) 102, 252, 278
MslI CAYNNNNRTG 2 cut(s) 149, 360
MspR9I CCNGG 2 cut(s) 71, 138
MvaI CCWGG 2 cut(s) 71, 138
NdeI CATATG 1 cut(s) 361
NdeII GATC 3 cut(s) 27, 91, 106
NlaIII CATG 1 cut(s) 80
NmuCI GTSAC 2 cut(s) 142, 370
OliI CACNNNNGTG 1 cut(s) 149
PfeI GAWTC 2 cut(s) 13, 332
PflFI GACNNNGTC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 25, 157, 393
PleI GAGTC 2 cut(s) 149, 367
PpsI GAGTC 2 cut(s) 149, 367
Psp6I CCWGG 2 cut(s) 69, 136
PspGI CCWGG 2 cut(s) 69, 136
PspPI GGNCC 2 cut(s) 73, 411
PsyI GACNNNGTC 1 cut(s) 77
RseI CAYNNNNRTG 2 cut(s) 149, 360
SaqAI TTAA 3 cut(s) 102, 252, 278
SatI GCNGC 3 cut(s) 24, 156, 392
Sau3AI GATC 3 cut(s) 27, 91, 106
Sau96I GGNCC 2 cut(s) 73, 411
SchI GAGTC 2 cut(s) 150, 367
ScrFI CCNGG 2 cut(s) 71, 138
SetI ASST 5 cut(s) 58, 160, 247, 399, 434
SfaNI GCATC 1 cut(s) 199
SinI GGWCC 2 cut(s) 73, 411
SmiMI CAYNNNNRTG 2 cut(s) 149, 360
Sse9I AATT 2 cut(s) 48, 229
SsiI CCGC 1 cut(s) 23
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 69, 136
TasI AATT 2 cut(s) 48, 229
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 2 cut(s) 13, 332
Tru1I TTAA 3 cut(s) 102, 252, 278
Tru9I TTAA 3 cut(s) 102, 252, 278
TseFI GTSAC 2 cut(s) 142, 370
TseI GCWGC 2 cut(s) 155, 391
Tsp45I GTSAC 2 cut(s) 142, 370
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 432
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 2 cut(s) 73, 411
XagI CCTNNNNNAGG 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 2 cut(s) 38, 421
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.