Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0001862

Physical Location & Seq

Genomic Coordinates Reverse (-)

35217716 .. 35221711

Gene Structure 3996 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034001

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGAGTCATCGCTTACACGTGTACGGCGAAGGCCTGGAGTTGTCCGCTTCGAGATACAAGTATCTTTCCAAACCCCCACAACAGTTATCTGGCAAAAAGCTCTGGTTGTCTCTCTCTCTCTCTCTCCCAACTGTGAATAACATGGAAGCCAAGCTTAAGTCTGTTACAGCGACGCTGTTTCTTTGTTTCCTCCTGTTTCCCTTGGTTTTTTCTGCTTCCAATGATGGGTTGCTCCGGGTTGGACTGAAAAAGAGGAAGTTTGATCAGAATAACCGGGTTGCTGCTAATCTTCAATCCAAGAATGGGGATGCTGTGAGAGCTGTTATCCGGAAATATAATCTTCGTGGAACTTTGGGAGATGATCAGGACATTGATATTGTATCTCTCAAGAATTACATGGATGCTCAGTACTTTGGGGAGATTGGTATTGGTACTCCCCCCCAGAAGTTCACTGTGATCTTTGATACTGGTAGCTCGAATTTGTGGGTGCCTTCTTCCAAGTGTTATTTCTCGATTGCATGCTATCTACATCCAAAGTACAAGTCGAGCAGTTCAACCACCTACAGCAAAAATGGTAAACCTGCAGCAATCCAATATGGAACTGGTGCAATCTCTGGCTTCTTCAGTGAAGACCATGTTACAGTTGGTGACCTCGTGGTGAAAGATCAGGAATTTATTGAGGCAACCAAGGAGCCCGGCATCACATTCTTGGTAGCCAAATTTGATGGAATACTTGGCCTTGGATTTCAAGAAATTTCAGTTGGAAATGCTGTTCCTGTGTGGTATAACATGGTCAAGCAGGGTCTTCTTAAGGAACCTGTTTTCTCATTCTGGTTTAACCGCAATGCTGATGAAGAAGTAGGAGGTGAAATTGTTTTTGGTGGAGTGGATCCTGATCATTATGTCGGAAAGCACACATATGTTCCTGTTACACAGAAAGGCTATTGGCAGTTTGACATGGGTGATGTTTTGATTGATGGTCAAACAACTGGATTTTGTGCCGGAGGCTGTTCAGCGATTGCTGATTCTGGAACTTCTCTGTTGGTTGGCCCAACGACAATTATTACTGAACTCAACCATGCCATTGGAGCCACCGGGATTGTAAGTCAAGAATGCAAGACTGTGGTTGCAGAATATGGAGATACCATAATTAAGATGATTTTAGCAAAGGACCAACCACAGAAAATCTGCTCTCAGATTGGGTTATGCACATTTGATGGGACTCGCGGTGTAAGTGTTGGAATCAAGAGTGTTGTGGACGAGGACAATCACAAGTCATCCGCTGGCTTATCTGATGCAATGTGTTCTGCTTGTGAGATGACTGTTGTATGGATGCAAAATCAGCTCAAGCAGAATCAAACACAGGATCGCATACTTGACTATGTGAATCAGCTCTGTGATCGGCTGCCTAGCCCAATGGGAGAATCTGCTGTTGATTGCGCTGGTTTGTCTTCCATGCCTAGTGTGTCCTTCACAATTGGTGGAAAACAATTTGATCTAGCCCCTGAGCAGTACGTGCTCAAAGTAGGCGAGGGAGAGGTAGCACAATGCATCAGTGGATTTACTGCTTTGGATGTCCCACCTCCTCGTGGACCACTCTGGATTCTGGGAGATGTGTTCATGGGTCAGTACCACACTGTGTTTGACTACGGCAATGAGAGAATTGGGTTTGCAGAAGCTGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

562

Amino Acids

61.06

Weight (kDa)

5.49

Isoelectric Point (pI)

32.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	136 - 560	7.3e-118	Eukaryotic aspartyl protease
TAXi_N	PF14543	137 - 294	1.4e-10	Xylanase inhibitor N-terminal
SapB_2	PF03489	370 - 403	2.5e-12	Saposin-like type B, region 2
SapB_1	PF05184	434 - 470	6.4e-14	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11910 AT1G11910 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290
fragaria_vesca	FvH4_3g23140 FvH4_3g23140 FvH4_6g42880 FvH4_6g42880
malus_domestica	MD00G1159300.v1.1 MD09G1111100.v1.1 MD11G1213600.v1.1 MD15G1413400.v1.1 MD17G1098900.v1.1 MD17G1282600.v1.1 MD17G1282700.v1.1
prunus_persica	Prupe.3G214900_v2.0.a1 Prupe.3G214900_v2.0.a1 Prupe.4G217700_v2.0.a1
pyrus_communis	pycom03g14920 pycom09g03360 pycom11g18820 pycom17g09430
rosa_chinensis	RchiOBHm_Chr2g0158811 RchiOBHm_Chr5g0040781
rosa_laevigata	RLG00000021115 RLG00000034001
rosa_multiflora	Rmu_sc0001579.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039910 Rroxscaffold_2G00090980
rosa_rugosa	Rorug02G0472700 Rorug05G0187200 Rorug05G0187300
rosa_samantha	Rh2AG538500 Rh2BG551200 Rh2CG521800 Rh2DG561000 Rh5AG273000 Rh5BG278100 Rh5CG310100 Rh5DG286000
rosa_wichuraiana	Rw2G044570 Rw5G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	589
AccB1I	GGYRCC	1 cut(s)	487
AccII	CGCG	1 cut(s)	1227
AccIII	TCCGGA	1 cut(s)	327
AciI	CCGC	4 cut(s)	45, 841, 1227, 1281
AclWI	GGATC	3 cut(s)	884, 897, 1374
AcsI	RAATTY	4 cut(s)	478, 671, 719, 753
AcuI	CTGAAG	1 cut(s)	607
AcvI	CACGTG	1 cut(s)	19
AdeI	CACNNNGTG	1 cut(s)	1639
AfaI	GTAC	6 cut(s)	23, 410, 433, 539, 1514, 1631
AfiI	CCNNNNNNNGG	3 cut(s)	225, 303, 1589
AflII	CTTAAG	2 cut(s)	155, 809
AflIII	ACRYGT	2 cut(s)	16, 18
AgsI	TTSAA	3 cut(s)	293, 555, 749
AjnI	CCWGG	1 cut(s)	33
AjuI	GAANNNNNNNTTGG	6 cut(s)	491, 523, 744, 776, 861, 893
AloI	GAACNNNNNNTCC	2 cut(s)	756, 788
AluBI	AGCT	7 cut(s)	100, 154, 320, 474, 1345, 1393, 1679
AluI	AGCT	7 cut(s)	100, 154, 320, 474, 1345, 1393, 1679
Alw21I	GWGCWC	1 cut(s)	1521
Alw26I	GTCTC	1 cut(s)	114
AlwI	GGATC	3 cut(s)	884, 897, 1374
AlwNI	CAGNNNCTG	1 cut(s)	1679
Aor13HI	TCCGGA	1 cut(s)	327
AoxI	GGCC	3 cut(s)	31, 736, 1048
ApeKI	GCWGC	4 cut(s)	281, 584, 1405, 1679
ApoI	RAATTY	4 cut(s)	478, 671, 719, 753
ArsI	GACNNNNNNTTYG	2 cut(s)	527, 559
AspLEI	GCGC	1 cut(s)	1442
AspS9I	GGNCC	3 cut(s)	1049, 1171, 1592
AsuC2I	CCSGG	4 cut(s)	236, 275, 696, 1096
AsuHPI	GGTGA	4 cut(s)	659, 670, 878, 974
AvaII	GGWCC	2 cut(s)	1171, 1592
BamHI	GGATCC	1 cut(s)	889
BanI	GGYRCC	1 cut(s)	487
BanII	GRGCYC	1 cut(s)	696
BauI	CACGAG	2 cut(s)	653, 1587
BbrPI	CACGTG	1 cut(s)	19
BbsI	GAAGAC	3 cut(s)	636, 797, 1443
Bbv12I	GWGCWC	1 cut(s)	1521
BbvI	GCAGC	4 cut(s)	268, 596, 1392, 1666
BccI	CCATC	4 cut(s)	218, 719, 971, 1211
BceAI	ACGGC	2 cut(s)	40, 1666
BciT130I	CCWGG	1 cut(s)	35
BclI	TGATCA	3 cut(s)	262, 361, 895
BcnI	CCSGG	4 cut(s)	236, 275, 696, 1096
BcoDI	GTCTC	1 cut(s)	114
BfaI	CTAG	3 cut(s)	1410, 1461, 1499
BfmI	CTRYAG	2 cut(s)	562, 582
BfrI	CTTAAG	2 cut(s)	155, 809
BfuAI	ACCTGC	1 cut(s)	589
BisI	GCNGC	4 cut(s)	282, 585, 1406, 1680
BlsI	GCNGC	4 cut(s)	283, 586, 1407, 1681
BmcAI	AGTACT	1 cut(s)	410
Bme1390I	CCNGG	5 cut(s)	35, 236, 275, 696, 1096
Bme18I	GGWCC	2 cut(s)	1171, 1592
BmgT120I	GGNCC	3 cut(s)	1049, 1171, 1592
BmiI	GGNNCC	5 cut(s)	489, 693, 816, 891, 1090
BmrFI	CCNGG	5 cut(s)	35, 236, 275, 696, 1096
BmsI	GCATC	6 cut(s)	298, 391, 708, 1285, 1323, 1560
BpiI	GAAGAC	3 cut(s)	636, 797, 1443
BpmI	CTGGAG	1 cut(s)	56
Bpu10I	CCTNAGC	1 cut(s)	1506
BpuEI	CTTGAG	2 cut(s)	371, 1331
BpuMI	CCSGG	4 cut(s)	236, 275, 696, 1096
BsaAI	YACGTR	2 cut(s)	19, 1516
BsaBI	GATNNNNATC	1 cut(s)	894
BsaJI	CCNNGG	3 cut(s)	201, 687, 739
BsaWI	WCCGGW	1 cut(s)	327
Bsc4I	CCNNNNNNNGG	3 cut(s)	225, 303, 1589
Bse1I	ACTGG	3 cut(s)	472, 607, 994
Bse3DI	GCAATG	3 cut(s)	850, 1305, 1660
Bse8I	GATNNNNATC	1 cut(s)	894
BseAI	TCCGGA	1 cut(s)	327
BseBI	CCWGG	1 cut(s)	35
BseDI	CCNNGG	3 cut(s)	201, 687, 739
BseGI	GGATG	6 cut(s)	313, 406, 529, 1277, 1338, 1579
BseJI	GATNNNNATC	1 cut(s)	894
BseLI	CCNNNNNNNGG	3 cut(s)	225, 303, 1589
BseMI	GCAATG	3 cut(s)	850, 1305, 1660
BseMII	CTCAG	3 cut(s)	419, 1208, 1497
BseNI	ACTGG	3 cut(s)	472, 607, 994
BseRI	GAGGAG	1 cut(s)	1575
BseXI	GCAGC	4 cut(s)	268, 596, 1392, 1666
Bsh1236I	CGCG	1 cut(s)	1227
BshFI	GGCC	3 cut(s)	33, 738, 1050
BshNI	GGYRCC	1 cut(s)	487
BsiHKAI	GWGCWC	1 cut(s)	1521
BsiSI	CCGG	6 cut(s)	235, 274, 328, 696, 1002, 1095
BslFI	GGGAC	2 cut(s)	1234, 1562
BslI	CCNNNNNNNGG	3 cut(s)	225, 303, 1589
BsmAI	GTCTC	1 cut(s)	114
BsmFI	GGGAC	2 cut(s)	1234, 1562
BsmI	GAATGC	1 cut(s)	1118
BsnI	GGCC	3 cut(s)	33, 738, 1050
Bsp1286I	GDGCHC	2 cut(s)	696, 1521
Bsp13I	TCCGGA	1 cut(s)	327
Bsp143I	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
BspACI	CCGC	4 cut(s)	45, 841, 1227, 1281
BspANI	GGCC	3 cut(s)	33, 738, 1050
BspCNI	CTCAG	3 cut(s)	418, 1207, 1498
BspEI	TCCGGA	1 cut(s)	327
BspFNI	CGCG	1 cut(s)	1227
BspLI	GGNNCC	5 cut(s)	489, 693, 816, 891, 1090
BspMAI	CTGCAG	1 cut(s)	586
BspMI	ACCTGC	1 cut(s)	589
BspPI	GGATC	3 cut(s)	884, 897, 1374
BspT107I	GGYRCC	1 cut(s)	487
BspTI	CTTAAG	2 cut(s)	155, 809
BsrDI	GCAATG	3 cut(s)	850, 1305, 1660
BsrI	ACTGG	3 cut(s)	472, 607, 994
BssECI	CCNNGG	3 cut(s)	201, 687, 739
BssMI	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
BssSI	CACGAG	2 cut(s)	653, 1587
BssT1I	CCWWGG	3 cut(s)	201, 687, 739
Bst2BI	CACGAG	2 cut(s)	653, 1587
Bst2UI	CCWGG	1 cut(s)	35
Bst4CI	ACNGT	7 cut(s)	84, 133, 454, 643, 1123, 1324, 1639
BstAFI	CTTAAG	2 cut(s)	155, 809
BstAPI	GCANNNNNTGC	2 cut(s)	1516, 1679
BstBAI	YACGTR	2 cut(s)	19, 1516
BstC8I	GCNNGC	2 cut(s)	520, 1285
BstDEI	CTNAG	3 cut(s)	405, 1194, 1506
BstEII	GGTNACC	1 cut(s)	647
BstF5I	GGATG	6 cut(s)	313, 406, 529, 1277, 1338, 1579
BstFNI	CGCG	1 cut(s)	1227
BstHHI	GCGC	1 cut(s)	1442
BstKTI	GATC	9 cut(s)	265, 364, 459, 667, 892, 898, 1369, 1402, 1498
BstMAI	GTCTC	1 cut(s)	114
BstMBI	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
BstMWI	GCNNNNNNNGC	5 cut(s)	317, 1088, 1342, 1516, 1679
BstNI	CCWGG	1 cut(s)	35
BstNSI	RCATGY	1 cut(s)	522
BstPI	GGTNACC	1 cut(s)	647
BstSCI	CCNGG	5 cut(s)	33, 234, 273, 694, 1094
BstSFI	CTRYAG	2 cut(s)	562, 582
BstUI	CGCG	1 cut(s)	1227
BstV1I	GCAGC	4 cut(s)	268, 596, 1392, 1666
BstV2I	GAAGAC	3 cut(s)	636, 797, 1443
BstX2I	RGATCY	1 cut(s)	889
BstXI	CCANNNNNNTGG	1 cut(s)	1085
BstYI	RGATCY	1 cut(s)	889
BsuRI	GGCC	3 cut(s)	33, 738, 1050
BtsCI	GGATG	6 cut(s)	313, 406, 529, 1277, 1338, 1579
BtsIMutI	CAGTG	4 cut(s)	450, 631, 1561, 1635
BveI	ACCTGC	1 cut(s)	589
Cac8I	GCNNGC	2 cut(s)	520, 1285
CaiI	CAGNNNCTG	1 cut(s)	1679
CfoI	GCGC	1 cut(s)	1442
Cfr13I	GGNCC	3 cut(s)	1049, 1171, 1592
CseI	GACGC	1 cut(s)	181
Csp6I	GTAC	6 cut(s)	22, 409, 432, 538, 1513, 1630
CspCI	CAANNNNNGTGG	2 cut(s)	1081, 1116
CviAII	CATG	9 cut(s)	142, 397, 519, 635, 790, 958, 1079, 1456, 1621
CviQI	GTAC	6 cut(s)	22, 409, 432, 538, 1513, 1630
DdeI	CTNAG	3 cut(s)	405, 1194, 1506
DpnI	GATC	9 cut(s)	264, 363, 458, 666, 891, 897, 1368, 1401, 1497
DpnII	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
DraIII	CACNNNGTG	1 cut(s)	1639
Eco130I	CCWWGG	3 cut(s)	201, 687, 739
Eco147I	AGGCCT	1 cut(s)	33
Eco24I	GRGCYC	1 cut(s)	696
Eco47I	GGWCC	2 cut(s)	1171, 1592
Eco57I	CTGAAG	1 cut(s)	607
Eco72I	CACGTG	1 cut(s)	19
Eco91I	GGTNACC	1 cut(s)	647
EcoO65I	GGTNACC	1 cut(s)	647
EcoRII	CCWGG	1 cut(s)	33
EcoT14I	CCWWGG	3 cut(s)	201, 687, 739
EcoT22I	ATGCAT	1 cut(s)	1553
EcoT38I	GRGCYC	1 cut(s)	696
ErhI	CCWWGG	3 cut(s)	201, 687, 739
FaeI	CATG	9 cut(s)	145, 400, 522, 638, 793, 961, 1082, 1459, 1624
FalI	AAGNNNNNCTT	2 cut(s)	138, 170
FaqI	GGGAC	2 cut(s)	1234, 1562
FatI	CATG	9 cut(s)	141, 396, 518, 634, 789, 957, 1078, 1455, 1620
FauNDI	CATATG	1 cut(s)	919
FbaI	TGATCA	3 cut(s)	262, 361, 895
Fnu4HI	GCNGC	4 cut(s)	282, 585, 1406, 1680
FokI	GGATG	6 cut(s)	320, 413, 516, 1264, 1345, 1586
FriOI	GRGCYC	1 cut(s)	696
Fsp4HI	GCNGC	4 cut(s)	282, 585, 1406, 1680
FspBI	CTAG	3 cut(s)	1410, 1461, 1499
GlaI	GCGC	1 cut(s)	1441
GluI	GCNGC	4 cut(s)	282, 585, 1406, 1680
GsuI	CTGGAG	1 cut(s)	56
HaeIII	GGCC	3 cut(s)	33, 738, 1050
HapII	CCGG	6 cut(s)	235, 274, 328, 696, 1002, 1095
HgaI	GACGC	1 cut(s)	181
HhaI	GCGC	1 cut(s)	1442
Hin1II	CATG	9 cut(s)	145, 400, 522, 638, 793, 961, 1082, 1459, 1624
Hin6I	GCGC	1 cut(s)	1440
HinP1I	GCGC	1 cut(s)	1440
HindIII	AAGCTT	1 cut(s)	152
HinfI	GANTC	8 cut(s)	4, 1025, 1222, 1242, 1354, 1387, 1424, 1603
HpaII	CCGG	6 cut(s)	235, 274, 328, 696, 1002, 1095
HphI	GGTGA	4 cut(s)	659, 670, 878, 974
Hpy166II	GTNNAC	5 cut(s)	22, 450, 578, 1258, 1592
Hpy188I	TCNGA	4 cut(s)	267, 908, 1197, 1294
Hpy8I	GTNNAC	5 cut(s)	22, 450, 578, 1258, 1592
Hpy99I	CGWCG	1 cut(s)	175
HpyAV	CCTTC	3 cut(s)	23, 501, 1480
HpyCH4III	ACNGT	7 cut(s)	84, 133, 454, 643, 1123, 1324, 1639
HpyCH4IV	ACGT	2 cut(s)	18, 1515
HpyF10VI	GCNNNNNNNGC	5 cut(s)	317, 1088, 1342, 1516, 1679
HpyF3I	CTNAG	3 cut(s)	405, 1194, 1506
HpySE526I	ACGT	2 cut(s)	18, 1515
Hsp92II	CATG	9 cut(s)	145, 400, 522, 638, 793, 961, 1082, 1459, 1624
HspAI	GCGC	1 cut(s)	1440
Kpn2I	TCCGGA	1 cut(s)	327
Ksp22I	TGATCA	3 cut(s)	262, 361, 895
Kzo9I	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
LmnI	GCTCC	3 cut(s)	237, 691, 1088
Lsp1109I	GCAGC	4 cut(s)	268, 596, 1392, 1666
LweI	GCATC	6 cut(s)	298, 391, 708, 1285, 1323, 1560
MaeI	CTAG	3 cut(s)	1410, 1461, 1499
MaeII	ACGT	2 cut(s)	18, 1515
MaeIII	GTNAC	4 cut(s)	163, 637, 647, 928
MalI	GATC	9 cut(s)	264, 363, 458, 666, 891, 897, 1368, 1401, 1497
MboI	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
MboII	GAAGA	8 cut(s)	281, 332, 486, 613, 641, 797, 866, 1443
MfeI	CAATTG	1 cut(s)	1476
MflI	RGATCY	1 cut(s)	889
MhlI	GDGCHC	2 cut(s)	696, 1521
MlyI	GAGTC	2 cut(s)	13, 1216
MmeI	TCCRAC	4 cut(s)	220, 742, 886, 1219
Mph1103I	ATGCAT	1 cut(s)	1553
MroI	TCCGGA	1 cut(s)	327
MseI	TTAA	4 cut(s)	156, 810, 837, 1152
MslI	CAYNNNNRTG	1 cut(s)	918
MspA1I	CMGCKG	1 cut(s)	1283
MspCI	CTTAAG	2 cut(s)	155, 809
MspI	CCGG	6 cut(s)	235, 274, 328, 696, 1002, 1095
MspR9I	CCNGG	5 cut(s)	35, 236, 275, 696, 1096
MunI	CAATTG	1 cut(s)	1476
Mva1269I	GAATGC	1 cut(s)	1118
MvaI	CCWGG	1 cut(s)	35
MvnI	CGCG	1 cut(s)	1227
MwoI	GCNNNNNNNGC	5 cut(s)	317, 1088, 1342, 1516, 1679
NciI	CCSGG	4 cut(s)	236, 275, 696, 1096
NdeI	CATATG	1 cut(s)	919
NdeII	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
NlaIII	CATG	9 cut(s)	145, 400, 522, 638, 793, 961, 1082, 1459, 1624
NlaIV	GGNNCC	5 cut(s)	489, 693, 816, 891, 1090
NmuCI	GTSAC	1 cut(s)	647
NsiI	ATGCAT	1 cut(s)	1553
NspI	RCATGY	1 cut(s)	522
PaeI	GCATGC	1 cut(s)	522
PceI	AGGCCT	1 cut(s)	33
PcsI	WCGNNNNNNNCGW	1 cut(s)	24
PctI	GAATGC	1 cut(s)	1118
PfeI	GAWTC	6 cut(s)	1025, 1242, 1354, 1387, 1424, 1603
PkrI	GCNGC	4 cut(s)	283, 586, 1407, 1681
PleI	GAGTC	2 cut(s)	12, 1216
PmaCI	CACGTG	1 cut(s)	19
PmlI	CACGTG	1 cut(s)	19
PpsI	GAGTC	2 cut(s)	12, 1216
Ppu21I	YACGTR	2 cut(s)	19, 1516
Psp6I	CCWGG	1 cut(s)	33
PspCI	CACGTG	1 cut(s)	19
PspEI	GGTNACC	1 cut(s)	647
PspGI	CCWGG	1 cut(s)	33
PspN4I	GGNNCC	5 cut(s)	489, 693, 816, 891, 1090
PspPI	GGNCC	3 cut(s)	1049, 1171, 1592
PstI	CTGCAG	1 cut(s)	586
PstNI	CAGNNNCTG	1 cut(s)	1679
PsuI	RGATCY	1 cut(s)	889
RsaI	GTAC	6 cut(s)	23, 410, 433, 539, 1514, 1631
RsaNI	GTAC	6 cut(s)	22, 409, 432, 538, 1513, 1630
RseI	CAYNNNNRTG	1 cut(s)	918
SaqAI	TTAA	4 cut(s)	156, 810, 837, 1152
SatI	GCNGC	4 cut(s)	282, 585, 1406, 1680
Sau3AI	GATC	9 cut(s)	262, 361, 456, 664, 889, 895, 1366, 1399, 1495
Sau96I	GGNCC	3 cut(s)	1049, 1171, 1592
ScaI	AGTACT	1 cut(s)	410
SchI	GAGTC	2 cut(s)	13, 1216
ScrFI	CCNGG	5 cut(s)	35, 236, 275, 696, 1096
SduI	GDGCHC	2 cut(s)	696, 1521
SfaNI	GCATC	6 cut(s)	298, 391, 708, 1285, 1323, 1560
SfcI	CTRYAG	2 cut(s)	562, 582
SinI	GGWCC	2 cut(s)	1171, 1592
SmiMI	CAYNNNNRTG	1 cut(s)	918
SmlI	CTYRAG	4 cut(s)	155, 386, 809, 1346
SmoI	CTYRAG	4 cut(s)	155, 386, 809, 1346
SphI	GCATGC	1 cut(s)	522
SseBI	AGGCCT	1 cut(s)	33
SsiI	CCGC	4 cut(s)	45, 841, 1227, 1281
SspMI	CTAG	3 cut(s)	1410, 1461, 1499
StuI	AGGCCT	1 cut(s)	33
StyD4I	CCNGG	5 cut(s)	33, 234, 273, 694, 1094
StyI	CCWWGG	3 cut(s)	201, 687, 739
TaaI	ACNGT	7 cut(s)	84, 133, 454, 643, 1123, 1324, 1639
TaiI	ACGT	2 cut(s)	21, 1518
TaqI	TCGA	4 cut(s)	50, 476, 512, 545
TatI	WGTACW	2 cut(s)	408, 537
TfiI	GAWTC	6 cut(s)	1025, 1242, 1354, 1387, 1424, 1603
Tru1I	TTAA	4 cut(s)	156, 810, 837, 1152
Tru9I	TTAA	4 cut(s)	156, 810, 837, 1152
TscAI	CASTG	4 cut(s)	457, 631, 1561, 1642
TseFI	GTSAC	1 cut(s)	647
TseI	GCWGC	4 cut(s)	281, 584, 1405, 1679
Tsp45I	GTSAC	1 cut(s)	647
TspDTI	ATGAA	2 cut(s)	867, 1609
TspRI	CASTG	4 cut(s)	457, 631, 1561, 1642
Vha464I	CTTAAG	2 cut(s)	155, 809
VpaK11BI	GGWCC	2 cut(s)	1171, 1592
XapI	RAATTY	4 cut(s)	478, 671, 719, 753
XceI	RCATGY	1 cut(s)	522
XcmI	CCANNNNNNNNNTGG	2 cut(s)	599, 641
XspI	CTAG	3 cut(s)	1410, 1461, 1499
ZrmI	AGTACT	1 cut(s)	410
Zsp2I	ATGCAT	1 cut(s)	1553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.