Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001862

Physical Location & Seq

Genomic Coordinates Forward (+)

74994577 .. 74999675

Gene Structure 5099 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ52748

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1551 bp

ATGGGTACCAAAATTAGGGCTTGTTTGGTGACTCTATTTCAGTTTCTGCTTCTGTCCCATGTGGTGCTCTCCACACCCAATGATGGGTTGGTTAGAATTGGACTTAAAAAGGTGAAATTGGATCAGACATCTCAGCTTCACAGGGAGACTAATGCTAAGAATGCACAAGGAAGTATGAGAGATCCTTTGAAGGAGTATCGTCTCCGCGGTGGGGATTCTTCTGGGGACACTGATATTGTTGAACTGAAGAACTATATGGATGCTCAGTATTTTGGGGAGATTGGTATTGGCACACCTGCTCAGAAGTTTACTGTGATATTTGATACTGGAAGTTCTAACCTTTGGGTGCCCTCGGCAAAGTGTTACTTCTCGGTGGCTTGCTTTTTTCACGCCAAGTATAAGTCTAGTCAGTCAAGTTCACACACAAAGAATGGGACATCTGCGGCTATCCAGTACGGAACTGGCGCAATTGCAGGTTTTTTTAGCCAGGACCATGTCACAGTTGGGAATCTTGTTGTTAAGGATCAAGATTTTATTGAGGCAACCAAGGAGCCTGGGATCACATTTGTGGCAGCTAAGTTTGATGGTATACTTGGACTTGGATTTCAAGAGATCTCAGTTGGAGGTGCTGTTCCTGTCTGGTATAATTTGGTCAATCAAGGTCTTGTTAAAGAACCAGTATTTTCATTTTGGTTAAATCGCAACACTGAAGGGGAAGAAGGGGGTGAGATTGTATTTGGTGGGATGGATTCTAACCATTACGTGGGTGAGCATACATATGTTCCAGTGACTCAAAAAGGCTATTGGCAGTTTGATATGGGGGATGTGCTAATTGGTGGTGAAACATCTGGACTTTGTGCTAGTGGTTGTTCAGCAATTGCTGATTCAGGAACTTCCCTATTGGCAGGGCCAACGACCATTATCACTCAAATAAATCATGCAATTGGAGCATCTGGTATTGTAAGCCAAGAGTGTAAGGCAGTTGTTGATCAGTATGGGAAAATTATACTGGAATTGTTGCTGGCTCAGGTTAAACCCCAAAGAATCTGCTCTCAAATTGGTGTATGTACTTTTGACGGGACTCGTGGTGTGAGTATGGGCATTGAAACTGTGGTGGATGAGAATATGGAAACTACATCTGATGGCGTGCATGATGCTGCATGTACTGCTTGTGAGATGGCAGTAGTATGGATACAGAATCAGCTAAAGCTAAATCTTAATCAGACAGAGGAGCAGATCTTGAACTATGCAAATGAGCTCTGTGAACGACTGCCCAGTCCAAGTGGTGAATCAGCTGTTCAATGTAACGCTCTCTCCTCCTTGCCCAGTATATCATTTACCATTGGTGGTAAAGTATTTGACCTTGCTCCAGAGCAGTACGTTCTCAAAGTGGGTGAGGGAGCTACGGCACAATGCCTTAGTGGATTTATAGCTCTAGATGTGGCTCCACCTCGAGGACCACTGTGGATTCTGGGAGATATTTTCATGGGTCAGTATCACACAGTGTTCGACTATGGGAAAGCTACTGTTGGATTTGCAGAAGCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

516

Amino Acids

55.38

Weight (kDa)

4.99

Isoelectric Point (pI)

31.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	89 - 515	6.7e-116	Eukaryotic aspartyl protease
TAXi_N	PF14543	90 - 248	5.9e-11	Xylanase inhibitor N-terminal
SapB_2	PF03489	323 - 356	5.1e-12	Saposin-like type B, region 2
SapB_1	PF05184	387 - 425	9.1e-10	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11910 AT1G11910 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290
fragaria_vesca	FvH4_3g23140 FvH4_3g23140 FvH4_6g42880 FvH4_6g42880
malus_domestica	MD00G1159300.v1.1 MD09G1111100.v1.1 MD11G1213600.v1.1 MD15G1413400.v1.1 MD17G1098900.v1.1 MD17G1282600.v1.1 MD17G1282700.v1.1
prunus_persica	Prupe.3G214900_v2.0.a1 Prupe.3G214900_v2.0.a1 Prupe.4G217700_v2.0.a1
pyrus_communis	pycom03g14920 pycom09g03360 pycom11g18820 pycom17g09430
rosa_chinensis	RchiOBHm_Chr2g0158811 RchiOBHm_Chr5g0040781
rosa_laevigata	RLG00000021115 RLG00000034001
rosa_multiflora	Rmu_sc0001579.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039910 Rroxscaffold_2G00090980
rosa_rugosa	Rorug02G0472700 Rorug05G0187200 Rorug05G0187300
rosa_samantha	Rh2AG538500 Rh2BG551200 Rh2CG521800 Rh2DG561000 Rh5AG273000 Rh5BG278100 Rh5CG310100 Rh5DG286000
rosa_wichuraiana	Rw2G044570 Rw5G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	304
AasI	GACNNNNNNGTC	1 cut(s)	1275
AbsI	CCTCGAGG	1 cut(s)	1452
Acc36I	ACCTGC	2 cut(s)	304, 464
Acc65I	GGTACC	1 cut(s)	5
AccB1I	GGYRCC	2 cut(s)	5, 346
AccB7I	CCANNNNNTGG	1 cut(s)	763
AccI	GTMKAC	1 cut(s)	589
AccII	CGCG	1 cut(s)	207
AciI	CCGC	3 cut(s)	205, 207, 443
AclWI	GGATC	4 cut(s)	129, 176, 531, 566
AcuI	CTGAAG	2 cut(s)	266, 729
AdeI	CACNNNGTG	1 cut(s)	1504
AfaI	GTAC	5 cut(s)	7, 455, 1069, 1165, 1379
AfiI	CCNNNNNNNGG	6 cut(s)	15, 83, 84, 211, 763, 1454
AgsI	TTSAA	6 cut(s)	190, 242, 608, 1106, 1243, 1301
AjnI	CCWGG	2 cut(s)	486, 553
AleI	CACNNNNGTG	1 cut(s)	566
AloI	GAACNNNNNNTCC	2 cut(s)	615, 647
Alw21I	GWGCWC	2 cut(s)	69, 1260
Alw26I	GTCTC	2 cut(s)	140, 206
AlwI	GGATC	4 cut(s)	129, 176, 531, 566
AlwNI	CAGNNNCTG	1 cut(s)	46
Ama87I	CYCGRG	1 cut(s)	1452
AoxI	GGCC	1 cut(s)	908
ApeKI	GCWGC	3 cut(s)	572, 1157, 1544
Asp718I	GGTACC	1 cut(s)	5
AspLEI	GCGC	1 cut(s)	467
AspS9I	GGNCC	3 cut(s)	490, 908, 1457
AsuHPI	GGTGA	7 cut(s)	40, 124, 737, 779, 851, 1298, 1406
AvaI	CYCGRG	1 cut(s)	1452
AvaII	GGWCC	2 cut(s)	490, 1457
BaeGI	GKGCMC	1 cut(s)	351
BanI	GGYRCC	2 cut(s)	5, 346
BanII	GRGCYC	1 cut(s)	1260
BauI	CACGAG	1 cut(s)	1083
Bbv12I	GWGCWC	2 cut(s)	69, 1260
BbvI	GCAGC	2 cut(s)	584, 1144
BccI	CCATC	5 cut(s)	77, 578, 739, 1136, 1171
BceAI	ACGGC	1 cut(s)	1422
BciT130I	CCWGG	2 cut(s)	488, 555
BciVI	GTATCC	1 cut(s)	1185
BclI	TGATCA	1 cut(s)	988
BcoDI	GTCTC	2 cut(s)	140, 206
BfaI	CTAG	3 cut(s)	405, 861, 1436
BfuAI	ACCTGC	2 cut(s)	304, 464
BfuI	GTATCC	1 cut(s)	1185
BglII	AGATCT	2 cut(s)	612, 1236
BisI	GCNGC	4 cut(s)	444, 573, 1158, 1545
BlsI	GCNGC	4 cut(s)	445, 574, 1159, 1546
Bme1390I	CCNGG	2 cut(s)	488, 555
Bme18I	GGWCC	2 cut(s)	490, 1457
BmeT110I	CYCGRG	1 cut(s)	1452
BmgT120I	GGNCC	3 cut(s)	490, 908, 1457
BmiI	GGNNCC	4 cut(s)	7, 348, 552, 1446
BmrFI	CCNGG	2 cut(s)	488, 555
BmrI	ACTGGG	2 cut(s)	1269, 1320
BmsI	GCATC	3 cut(s)	250, 959, 1144
BmuI	ACTGGG	2 cut(s)	1269, 1320
BpmI	CTGGAG	1 cut(s)	1353
Bpu10I	CCTNAGC	1 cut(s)	1026
BsaAI	YACGTR	1 cut(s)	763
BsaJI	CCNNGG	4 cut(s)	205, 351, 546, 554
BsaXI	ACNNNNNCTCC	4 cut(s)	615, 645, 1298, 1328
Bsc4I	CCNNNNNNNGG	6 cut(s)	15, 83, 84, 211, 763, 1454
Bse1I	ACTGG	8 cut(s)	331, 451, 466, 677, 785, 1014, 1275, 1326
BseBI	CCWGG	2 cut(s)	488, 555
BseDI	CCNNGG	4 cut(s)	205, 351, 546, 554
BseGI	GGATG	4 cut(s)	265, 750, 829, 1123
BseLI	CCNNNNNNNGG	6 cut(s)	15, 83, 84, 211, 763, 1454
BseMII	CTCAG	5 cut(s)	146, 278, 314, 630, 1040
BseNI	ACTGG	8 cut(s)	331, 451, 466, 677, 785, 1014, 1275, 1326
BseRI	GAGGAG	2 cut(s)	1244, 1306
BseSI	GKGCMC	1 cut(s)	351
BseXI	GCAGC	2 cut(s)	584, 1144
Bsh1236I	CGCG	1 cut(s)	207
BshFI	GGCC	1 cut(s)	910
BshNI	GGYRCC	2 cut(s)	5, 346
BsiHKAI	GWGCWC	2 cut(s)	69, 1260
BsiHKCI	CYCGRG	1 cut(s)	1452
BslFI	GGGAC	4 cut(s)	40, 239, 448, 1093
BslI	CCNNNNNNNGG	6 cut(s)	15, 83, 84, 211, 763, 1454
BsmAI	GTCTC	2 cut(s)	140, 206
BsmBI	CGTCTC	1 cut(s)	206
BsmFI	GGGAC	4 cut(s)	40, 239, 448, 1093
BsmI	GAATGC	1 cut(s)	166
BsnI	GGCC	1 cut(s)	910
BsoBI	CYCGRG	1 cut(s)	1452
Bsp1286I	GDGCHC	3 cut(s)	69, 351, 1260
Bsp143I	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
BspACI	CCGC	3 cut(s)	205, 207, 443
BspANI	GGCC	1 cut(s)	910
BspCNI	CTCAG	5 cut(s)	145, 277, 313, 629, 1039
BspFNI	CGCG	1 cut(s)	207
BspLI	GGNNCC	4 cut(s)	7, 348, 552, 1446
BspMI	ACCTGC	2 cut(s)	304, 464
BspPI	GGATC	4 cut(s)	129, 176, 531, 566
BspT107I	GGYRCC	2 cut(s)	5, 346
BsrI	ACTGG	8 cut(s)	331, 451, 466, 677, 785, 1014, 1275, 1326
BssECI	CCNNGG	4 cut(s)	205, 351, 546, 554
BssMI	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
BssNAI	GTATAC	1 cut(s)	590
BssSI	CACGAG	1 cut(s)	1083
BssT1I	CCWWGG	1 cut(s)	546
Bst1107I	GTATAC	1 cut(s)	590
Bst2BI	CACGAG	1 cut(s)	1083
Bst2UI	CCWGG	2 cut(s)	488, 555
Bst4CI	ACNGT	6 cut(s)	313, 502, 1111, 1464, 1504, 1528
BstAPI	GCANNNNNTGC	1 cut(s)	1166
BstBAI	YACGTR	1 cut(s)	763
BstC8I	GCNNGC	3 cut(s)	379, 1023, 1148
BstDEI	CTNAG	9 cut(s)	132, 156, 264, 300, 576, 616, 1026, 1418, 1548
BstDSI	CCRYGG	1 cut(s)	205
BstF5I	GGATG	4 cut(s)	265, 750, 829, 1123
BstFNI	CGCG	1 cut(s)	207
BstHHI	GCGC	1 cut(s)	467
BstKTI	GATC	7 cut(s)	124, 184, 526, 561, 615, 991, 1239
BstMAI	GTCTC	2 cut(s)	140, 206
BstMBI	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
BstMWI	GCNNNNNNNGC	4 cut(s)	161, 947, 1166, 1544
BstNI	CCWGG	2 cut(s)	488, 555
BstNSI	RCATGY	1 cut(s)	1164
BstSCI	CCNGG	2 cut(s)	486, 553
BstSLI	GKGCMC	1 cut(s)	351
BstUI	CGCG	1 cut(s)	207
BstV1I	GCAGC	2 cut(s)	584, 1144
BstX2I	RGATCY	3 cut(s)	181, 612, 1236
BstYI	RGATCY	3 cut(s)	181, 612, 1236
BstZ17I	GTATAC	1 cut(s)	590
BsuI	GTATCC	1 cut(s)	1185
BsuRI	GGCC	1 cut(s)	910
BtgI	CCRYGG	1 cut(s)	205
BtsCI	GGATG	4 cut(s)	265, 750, 829, 1123
BtsIMutI	CAGTG	5 cut(s)	228, 705, 792, 1460, 1509
BveI	ACCTGC	2 cut(s)	304, 464
Cac8I	GCNNGC	3 cut(s)	379, 1023, 1148
CaiI	CAGNNNCTG	1 cut(s)	46
CfoI	GCGC	1 cut(s)	467
Cfr13I	GGNCC	3 cut(s)	490, 908, 1457
Cfr42I	CCGCGG	1 cut(s)	208
Csp6I	GTAC	5 cut(s)	6, 454, 1068, 1164, 1378
CviAII	CATG	6 cut(s)	59, 494, 938, 1151, 1161, 1486
CviQI	GTAC	5 cut(s)	6, 454, 1068, 1164, 1378
DdeI	CTNAG	9 cut(s)	132, 156, 264, 300, 576, 616, 1026, 1418, 1548
DpnI	GATC	7 cut(s)	123, 183, 525, 560, 614, 990, 1238
DpnII	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
DraIII	CACNNNGTG	1 cut(s)	1504
DrdI	GACNNNNNNGTC	1 cut(s)	1275
DseDI	GACNNNNNNGTC	1 cut(s)	1275
Ecl136II	GAGCTC	1 cut(s)	1258
Eco130I	CCWWGG	1 cut(s)	546
Eco24I	GRGCYC	1 cut(s)	1260
Eco47I	GGWCC	2 cut(s)	490, 1457
Eco53kI	GAGCTC	1 cut(s)	1258
Eco57I	CTGAAG	2 cut(s)	266, 729
Eco88I	CYCGRG	1 cut(s)	1452
EcoICRI	GAGCTC	1 cut(s)	1258
EcoRII	CCWGG	2 cut(s)	486, 553
EcoT14I	CCWWGG	1 cut(s)	546
EcoT38I	GRGCYC	1 cut(s)	1260
ErhI	CCWWGG	1 cut(s)	546
Esp3I	CGTCTC	1 cut(s)	206
FaeI	CATG	6 cut(s)	62, 497, 941, 1154, 1164, 1489
FalI	AAGNNNNNCTT	2 cut(s)	350, 382
FaqI	GGGAC	4 cut(s)	40, 239, 448, 1093
FatI	CATG	6 cut(s)	58, 493, 937, 1150, 1160, 1485
FauNDI	CATATG	1 cut(s)	778
FbaI	TGATCA	1 cut(s)	988
FblI	GTMKAC	1 cut(s)	589
Fnu4HI	GCNGC	4 cut(s)	444, 573, 1158, 1545
FokI	GGATG	4 cut(s)	272, 757, 836, 1130
FriOI	GRGCYC	1 cut(s)	1260
Fsp4HI	GCNGC	4 cut(s)	444, 573, 1158, 1545
FspBI	CTAG	3 cut(s)	405, 861, 1436
GlaI	GCGC	1 cut(s)	466
GluI	GCNGC	4 cut(s)	444, 573, 1158, 1545
GsuI	CTGGAG	1 cut(s)	1353
HaeIII	GGCC	1 cut(s)	910
HhaI	GCGC	1 cut(s)	467
Hin1II	CATG	6 cut(s)	62, 497, 941, 1154, 1164, 1489
Hin6I	GCGC	1 cut(s)	465
HinP1I	GCGC	1 cut(s)	465
HphI	GGTGA	7 cut(s)	40, 124, 737, 779, 851, 1298, 1406
Hpy166II	GTNNAC	4 cut(s)	309, 419, 590, 1265
Hpy188I	TCNGA	4 cut(s)	126, 303, 1141, 1224
Hpy188III	TCNNGA	7 cut(s)	527, 608, 849, 888, 1240, 1370, 1436
Hpy8I	GTNNAC	4 cut(s)	309, 419, 590, 1265
HpyAV	CCTTC	3 cut(s)	184, 704, 713
HpyCH4III	ACNGT	6 cut(s)	313, 502, 1111, 1464, 1504, 1528
HpyCH4IV	ACGT	2 cut(s)	762, 1380
HpyCH4V	TGCA	7 cut(s)	164, 473, 941, 1150, 1160, 1250, 1538
HpyF10VI	GCNNNNNNNGC	4 cut(s)	161, 947, 1166, 1544
HpyF3I	CTNAG	9 cut(s)	132, 156, 264, 300, 576, 616, 1026, 1418, 1548
HpySE526I	ACGT	2 cut(s)	762, 1380
Hsp92II	CATG	6 cut(s)	62, 497, 941, 1154, 1164, 1489
HspAI	GCGC	1 cut(s)	465
KpnI	GGTACC	1 cut(s)	9
Ksp22I	TGATCA	1 cut(s)	988
KspI	CCGCGG	1 cut(s)	208
Kzo9I	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
LmnI	GCTCC	6 cut(s)	550, 947, 1231, 1372, 1400, 1450
Lsp1109I	GCAGC	2 cut(s)	584, 1144
LweI	GCATC	3 cut(s)	250, 959, 1144
MaeI	CTAG	3 cut(s)	405, 861, 1436
MaeII	ACGT	2 cut(s)	762, 1380
MaeIII	GTNAC	5 cut(s)	28, 362, 496, 787, 1304
MalI	GATC	7 cut(s)	123, 183, 525, 560, 614, 990, 1238
MboI	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
MboII	GAAGA	3 cut(s)	210, 259, 728
MfeI	CAATTG	3 cut(s)	468, 876, 942
MflI	RGATCY	3 cut(s)	181, 612, 1236
MhlI	GDGCHC	3 cut(s)	69, 351, 1260
MlyI	GAGTC	3 cut(s)	25, 784, 1075
MmeI	TCCRAC	2 cut(s)	601, 1510
MnlI	CCTC	8 cut(s)	361, 532, 617, 1222, 1327, 1390, 1448, 1461
MseI	TTAA	6 cut(s)	105, 519, 669, 695, 1032, 1218
MslI	CAYNNNNRTG	2 cut(s)	566, 777
MspA1I	CMGCKG	2 cut(s)	207, 1295
MspR9I	CCNGG	2 cut(s)	488, 555
MunI	CAATTG	3 cut(s)	468, 876, 942
Mva1269I	GAATGC	1 cut(s)	166
MvaI	CCWGG	2 cut(s)	488, 555
MvnI	CGCG	1 cut(s)	207
MwoI	GCNNNNNNNGC	4 cut(s)	161, 947, 1166, 1544
NdeI	CATATG	1 cut(s)	778
NdeII	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
NlaIII	CATG	6 cut(s)	62, 497, 941, 1154, 1164, 1489
NlaIV	GGNNCC	4 cut(s)	7, 348, 552, 1446
NmeAIII	GCCGAG	1 cut(s)	332
NmuCI	GTSAC	3 cut(s)	28, 496, 787
NspI	RCATGY	1 cut(s)	1164
OliI	CACNNNNGTG	1 cut(s)	566
PaeR7I	CTCGAG	1 cut(s)	1452
PaqCI	CACCTGC	1 cut(s)	304
PctI	GAATGC	1 cut(s)	166
PfeI	GAWTC	8 cut(s)	215, 508, 749, 884, 1044, 1198, 1289, 1468
PflFI	GACNNNGTC	1 cut(s)	494
PflMI	CCANNNNNTGG	1 cut(s)	763
PkrI	GCNGC	4 cut(s)	445, 574, 1159, 1546
PleI	GAGTC	3 cut(s)	25, 784, 1075
PpsI	GAGTC	3 cut(s)	25, 784, 1075
Ppu21I	YACGTR	1 cut(s)	763
Psp124BI	GAGCTC	1 cut(s)	1260
Psp6I	CCWGG	2 cut(s)	486, 553
PspGI	CCWGG	2 cut(s)	486, 553
PspN4I	GGNNCC	4 cut(s)	7, 348, 552, 1446
PspPI	GGNCC	3 cut(s)	490, 908, 1457
PspXI	VCTCGAGB	1 cut(s)	1452
PstNI	CAGNNNCTG	1 cut(s)	46
PsuI	RGATCY	3 cut(s)	181, 612, 1236
PsyI	GACNNNGTC	1 cut(s)	494
PvuII	CAGCTG	1 cut(s)	1295
RsaI	GTAC	5 cut(s)	7, 455, 1069, 1165, 1379
RsaNI	GTAC	5 cut(s)	6, 454, 1068, 1164, 1378
RseI	CAYNNNNRTG	2 cut(s)	566, 777
SacI	GAGCTC	1 cut(s)	1260
SacII	CCGCGG	1 cut(s)	208
SaqAI	TTAA	6 cut(s)	105, 519, 669, 695, 1032, 1218
SatI	GCNGC	4 cut(s)	444, 573, 1158, 1545
Sau3AI	GATC	7 cut(s)	121, 181, 523, 558, 612, 988, 1236
Sau96I	GGNCC	3 cut(s)	490, 908, 1457
SchI	GAGTC	3 cut(s)	25, 784, 1075
ScrFI	CCNGG	2 cut(s)	488, 555
SduI	GDGCHC	3 cut(s)	69, 351, 1260
SfaNI	GCATC	3 cut(s)	250, 959, 1144
Sfr274I	CTCGAG	1 cut(s)	1452
Sfr303I	CCGCGG	1 cut(s)	208
SgrBI	CCGCGG	1 cut(s)	208
SinI	GGWCC	2 cut(s)	490, 1457
SlaI	CTCGAG	1 cut(s)	1452
SmiMI	CAYNNNNRTG	2 cut(s)	566, 777
SmlI	CTYRAG	1 cut(s)	1452
SmoI	CTYRAG	1 cut(s)	1452
SsiI	CCGC	3 cut(s)	205, 207, 443
SspMI	CTAG	3 cut(s)	405, 861, 1436
SstI	GAGCTC	1 cut(s)	1260
StyD4I	CCNGG	2 cut(s)	486, 553
StyI	CCWWGG	1 cut(s)	546
TaaI	ACNGT	6 cut(s)	313, 502, 1111, 1464, 1504, 1528
TaiI	ACGT	2 cut(s)	765, 1383
TaqI	TCGA	2 cut(s)	1453, 1509
TatI	WGTACW	2 cut(s)	1067, 1163
TauI	GCSGC	1 cut(s)	446
TfiI	GAWTC	8 cut(s)	215, 508, 749, 884, 1044, 1198, 1289, 1468
Tru1I	TTAA	6 cut(s)	105, 519, 669, 695, 1032, 1218
Tru9I	TTAA	6 cut(s)	105, 519, 669, 695, 1032, 1218
TscAI	CASTG	5 cut(s)	235, 712, 792, 1467, 1509
TseFI	GTSAC	3 cut(s)	28, 496, 787
TseI	GCWGC	3 cut(s)	572, 1157, 1544
Tsp45I	GTSAC	3 cut(s)	28, 496, 787
TspDTI	ATGAA	2 cut(s)	675, 1474
TspGWI	ACGGA	1 cut(s)	471
TspRI	CASTG	5 cut(s)	235, 712, 792, 1467, 1509
Tth111I	GACNNNGTC	1 cut(s)	494
Van91I	CCANNNNNTGG	1 cut(s)	763
VpaK11BI	GGWCC	2 cut(s)	490, 1457
XbaI	TCTAGA	1 cut(s)	1435
XceI	RCATGY	1 cut(s)	1164
XcmI	CCANNNNNNNNNTGG	3 cut(s)	85, 458, 500
XhoI	CTCGAG	1 cut(s)	1452
XmiI	GTMKAC	1 cut(s)	589
XspI	CTAG	3 cut(s)	405, 861, 1436

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.