Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001862

Physical Location & Seq

Genomic Coordinates Forward (+)

72089213 .. 72094769

Gene Structure 5557 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000021115

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGTTTCCTCGGAAGCAACTCTCAGTGTTCGTCTTCTCCCAGGAGCCCAACTCTGATCACTCAGAATTTCTGAAAATGGGTACCAAAATTAGGGCTTGTGTGGTGACTCTATTTCAGTTTCTGCTTCTGTCCCATGTGGTGCTCTCCACACCCAATGATGGGTTGGTTAGAATTGGACTTAAAAAGGTGAAATTGGATCAGACATCTCAGCTTCACAGGGAGACTAATGCTAAGAATGCACAAGGAAGTATGAGAGATCCTTTGAAGGTGTATCGTCTCCGCGGTGGGGATTCTTCTGGGGACACTGATATTGTTGAACTGAAAAACTATATGGATGCTCAGTATTTTGGGGAGATTGGTATTGGCACACCTGCTCAGAAGTTTACTGTGATATTTGATACTGGAAGTTCTAACCTTTGGGTGCCCTCGGCAAAGTGTTACTTCTCGGTGGCTTGCTTTTTTCACTCCAAGTATAAGTCTAGTCAGTCAAGTTCACACACAAAGAATGGGACATCTGCGGCTATCCAGTATGGAACTGGCGCAATTGCAGGTTTTTTTAGCCAGGACCATGTCACAGTTGGGAATCTTGTTGTTAAGGATCAAGATTTTATTGAGGCAACCAAGGAGCCTGGGATCACATTTGTGGCAGCTAAGTTTGATGGCATACTTGGACTTGGATTTCAAGAGATCTCAGTTGGAGGTGCTGTTCCAGTCTGGTATAATTTGGTCAATCAAGGTCTTGTTAAAGAACCAGTATTTTCATTTTGGTTAAATCGCAACACTGAAGGGGAAGAAGGGGGTGAGATTGTATTTGGTGGGATGGATTCTAACCATTACGTGGGTGAGCATACATATGTTCCAGTGACTCAAAAAGGCTATTGGCAGTTTGATATGGGGGATGTGCTAATTGGTGGTGAAACATCTGGACTTTGTGCTAGTGGTTGTTCAGCAATTGCTGATTCAGGAACTTCCCTATTGGCAGGGCCAACGACCATTATCACTCAAATAAATCATGCAATTGGAGCATCTGGTATTGTAAGCCAAGAGTGTAAGGCAGTTGTTGACCAGTATGGGAAAATTATACTGGAATTGTTGCTGGCTCAGGTTAAACCCCAAAGAATCTGCTCTCAAATTGGTGTATGTACTTTTGACGGGACTCGTGGTGTGAGTATGGGCATTGAAACTGTGGTGGATGAGAATATGGAAACTACATCTGATGGTGTGCATGATGCTGCATGTACTGCTTGTGAGATGGCAGTAGTATGGATACAGAATCAGCTAAAGCTAAATCTTAATCAGACAGAGGAGCAGATCTTGAACTATGCAAATGAGCTCTGTGAACGACTGCCCAGTCCAAGTGGTGAATCAGCTGTTCAATGTAACGCTCTCTCCTCCTTGCCCAGTATATCATTTACCATCGGTGGTAAAGTATTTGACCTTGCTCCAGAGCAGTATGTTCTCAAAGTGGGTGAGGGAGCTACGGCACAATGCCTTAGTGGATTTATAGCTCTAGATGTGGCTCCACCTCGAGGACCACTGTGGATTCTGGGAGATATTTTCATGGGTCCATATCACACAGTGTTCGACTATGGGAAAGCTACTGTTGGATTTGCAGAAGCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

542

Amino Acids

58.3

Weight (kDa)

5.11

Isoelectric Point (pI)

32.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	114 - 540	1.1e-115	Eukaryotic aspartyl protease
TAXi_N	PF14543	115 - 273	5.6e-11	Xylanase inhibitor N-terminal
SapB_2	PF03489	348 - 381	5.5e-12	Saposin-like type B, region 2
SapB_1	PF05184	412 - 450	9.7e-10	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11910 AT1G11910 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290
fragaria_vesca	FvH4_3g23140 FvH4_3g23140 FvH4_6g42880 FvH4_6g42880
malus_domestica	MD00G1159300.v1.1 MD09G1111100.v1.1 MD11G1213600.v1.1 MD15G1413400.v1.1 MD17G1098900.v1.1 MD17G1282600.v1.1 MD17G1282700.v1.1
prunus_persica	Prupe.3G214900_v2.0.a1 Prupe.3G214900_v2.0.a1 Prupe.4G217700_v2.0.a1
pyrus_communis	pycom03g14920 pycom09g03360 pycom11g18820 pycom17g09430
rosa_chinensis	RchiOBHm_Chr2g0158811 RchiOBHm_Chr5g0040781
rosa_laevigata	RLG00000021115 RLG00000034001
rosa_multiflora	Rmu_sc0001579.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039910 Rroxscaffold_2G00090980
rosa_rugosa	Rorug02G0472700 Rorug05G0187200 Rorug05G0187300
rosa_samantha	Rh2AG538500 Rh2BG551200 Rh2CG521800 Rh2DG561000 Rh5AG273000 Rh5BG278100 Rh5CG310100 Rh5DG286000
rosa_wichuraiana	Rw2G044570 Rw5G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	379
AasI	GACNNNNNNGTC	1 cut(s)	1350
AbsI	CCTCGAGG	1 cut(s)	1527
Acc36I	ACCTGC	2 cut(s)	379, 539
Acc65I	GGTACC	1 cut(s)	80
AccB1I	GGYRCC	2 cut(s)	80, 421
AccB7I	CCANNNNNTGG	1 cut(s)	838
AccII	CGCG	1 cut(s)	282
AciI	CCGC	3 cut(s)	280, 282, 518
AclWI	GGATC	4 cut(s)	204, 251, 606, 641
AcsI	RAATTY	1 cut(s)	65
AcuI	CTGAAG	1 cut(s)	804
AdeI	CACNNNGTG	1 cut(s)	1579
AfaI	GTAC	3 cut(s)	82, 1144, 1240
AfiI	CCNNNNNNNGG	6 cut(s)	90, 158, 159, 286, 838, 1529
AgsI	TTSAA	6 cut(s)	265, 317, 683, 1181, 1318, 1376
AjnI	CCWGG	3 cut(s)	39, 561, 628
AleI	CACNNNNGTG	1 cut(s)	641
AloI	GAACNNNNNNTCC	2 cut(s)	690, 722
Alw21I	GWGCWC	2 cut(s)	144, 1335
Alw26I	GTCTC	2 cut(s)	215, 281
AlwI	GGATC	4 cut(s)	204, 251, 606, 641
AlwNI	CAGNNNCTG	1 cut(s)	121
Ama87I	CYCGRG	1 cut(s)	1527
AoxI	GGCC	1 cut(s)	983
ApeKI	GCWGC	3 cut(s)	647, 1232, 1619
ApoI	RAATTY	1 cut(s)	65
Asp718I	GGTACC	1 cut(s)	80
AspLEI	GCGC	1 cut(s)	542
AspS9I	GGNCC	4 cut(s)	565, 983, 1532, 1565
AsuHPI	GGTGA	7 cut(s)	115, 199, 812, 854, 926, 1373, 1481
AvaI	CYCGRG	1 cut(s)	1527
AvaII	GGWCC	3 cut(s)	565, 1532, 1565
BaeGI	GKGCMC	1 cut(s)	426
BanI	GGYRCC	2 cut(s)	80, 421
BanII	GRGCYC	2 cut(s)	48, 1335
BauI	CACGAG	1 cut(s)	1158
BbsI	GAAGAC	1 cut(s)	25
Bbv12I	GWGCWC	2 cut(s)	144, 1335
BbvI	GCAGC	2 cut(s)	659, 1219
BccI	CCATC	6 cut(s)	152, 653, 814, 1211, 1246, 1424
BceAI	ACGGC	1 cut(s)	1497
BciT130I	CCWGG	3 cut(s)	41, 563, 630
BciVI	GTATCC	1 cut(s)	1260
BclI	TGATCA	1 cut(s)	55
BcoDI	GTCTC	2 cut(s)	215, 281
BfaI	CTAG	3 cut(s)	480, 936, 1511
BfuAI	ACCTGC	2 cut(s)	379, 539
BfuI	GTATCC	1 cut(s)	1260
BglII	AGATCT	2 cut(s)	687, 1311
BisI	GCNGC	4 cut(s)	519, 648, 1233, 1620
BlsI	GCNGC	4 cut(s)	520, 649, 1234, 1621
Bme1390I	CCNGG	3 cut(s)	41, 563, 630
Bme18I	GGWCC	3 cut(s)	565, 1532, 1565
BmeT110I	CYCGRG	1 cut(s)	1527
BmgT120I	GGNCC	4 cut(s)	565, 983, 1532, 1565
BmiI	GGNNCC	6 cut(s)	45, 82, 423, 627, 1521, 1566
BmrFI	CCNGG	3 cut(s)	41, 563, 630
BmrI	ACTGGG	2 cut(s)	1344, 1395
BmsI	GCATC	3 cut(s)	325, 1034, 1219
BmuI	ACTGGG	2 cut(s)	1344, 1395
BpiI	GAAGAC	1 cut(s)	25
BplI	GAGNNNNNCTC	2 cut(s)	35, 67
BpmI	CTGGAG	1 cut(s)	1428
Bpu10I	CCTNAGC	1 cut(s)	1101
BsaAI	YACGTR	1 cut(s)	838
BsaJI	CCNNGG	6 cut(s)	8, 39, 280, 426, 621, 629
BsaXI	ACNNNNNCTCC	4 cut(s)	690, 720, 1373, 1403
Bsc4I	CCNNNNNNNGG	6 cut(s)	90, 158, 159, 286, 838, 1529
BseBI	CCWGG	3 cut(s)	41, 563, 630
BseDI	CCNNGG	6 cut(s)	8, 39, 280, 426, 621, 629
BseGI	GGATG	4 cut(s)	340, 825, 904, 1198
BseLI	CCNNNNNNNGG	6 cut(s)	90, 158, 159, 286, 838, 1529
BseMII	CTCAG	7 cut(s)	36, 75, 221, 353, 389, 705, 1115
BseRI	GAGGAG	2 cut(s)	1319, 1381
BseSI	GKGCMC	1 cut(s)	426
BseXI	GCAGC	2 cut(s)	659, 1219
Bsh1236I	CGCG	1 cut(s)	282
BshFI	GGCC	1 cut(s)	985
BshNI	GGYRCC	2 cut(s)	80, 421
BsiHKAI	GWGCWC	2 cut(s)	144, 1335
BsiHKCI	CYCGRG	1 cut(s)	1527
BslFI	GGGAC	4 cut(s)	115, 314, 523, 1168
BslI	CCNNNNNNNGG	6 cut(s)	90, 158, 159, 286, 838, 1529
BsmAI	GTCTC	2 cut(s)	215, 281
BsmBI	CGTCTC	1 cut(s)	281
BsmFI	GGGAC	4 cut(s)	115, 314, 523, 1168
BsmI	GAATGC	1 cut(s)	241
BsnI	GGCC	1 cut(s)	985
BsoBI	CYCGRG	1 cut(s)	1527
Bsp1286I	GDGCHC	4 cut(s)	48, 144, 426, 1335
Bsp143I	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
BspACI	CCGC	3 cut(s)	280, 282, 518
BspANI	GGCC	1 cut(s)	985
BspCNI	CTCAG	7 cut(s)	35, 74, 220, 352, 388, 704, 1114
BspFNI	CGCG	1 cut(s)	282
BspLI	GGNNCC	6 cut(s)	45, 82, 423, 627, 1521, 1566
BspMI	ACCTGC	2 cut(s)	379, 539
BspPI	GGATC	4 cut(s)	204, 251, 606, 641
BspT107I	GGYRCC	2 cut(s)	80, 421
BssECI	CCNNGG	6 cut(s)	8, 39, 280, 426, 621, 629
BssMI	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
BssSI	CACGAG	1 cut(s)	1158
BssT1I	CCWWGG	1 cut(s)	621
Bst2BI	CACGAG	1 cut(s)	1158
Bst2UI	CCWGG	3 cut(s)	41, 563, 630
Bst4CI	ACNGT	6 cut(s)	388, 577, 1186, 1539, 1579, 1603
BstAPI	GCANNNNNTGC	1 cut(s)	1241
BstBAI	YACGTR	1 cut(s)	838
BstC8I	GCNNGC	2 cut(s)	454, 1098
BstDSI	CCRYGG	1 cut(s)	280
BstF5I	GGATG	4 cut(s)	340, 825, 904, 1198
BstFNI	CGCG	1 cut(s)	282
BstHHI	GCGC	1 cut(s)	542
BstKTI	GATC	7 cut(s)	58, 199, 259, 601, 636, 690, 1314
BstMAI	GTCTC	2 cut(s)	215, 281
BstMBI	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
BstMWI	GCNNNNNNNGC	4 cut(s)	236, 1022, 1241, 1619
BstNI	CCWGG	3 cut(s)	41, 563, 630
BstNSI	RCATGY	1 cut(s)	1239
BstSCI	CCNGG	3 cut(s)	39, 561, 628
BstSLI	GKGCMC	1 cut(s)	426
BstUI	CGCG	1 cut(s)	282
BstV1I	GCAGC	2 cut(s)	659, 1219
BstV2I	GAAGAC	1 cut(s)	25
BstX2I	RGATCY	3 cut(s)	256, 687, 1311
BstYI	RGATCY	3 cut(s)	256, 687, 1311
BsuI	GTATCC	1 cut(s)	1260
BsuRI	GGCC	1 cut(s)	985
BtgI	CCRYGG	1 cut(s)	280
BtsCI	GGATG	4 cut(s)	340, 825, 904, 1198
BtsIMutI	CAGTG	6 cut(s)	30, 303, 780, 867, 1535, 1584
BveI	ACCTGC	2 cut(s)	379, 539
Cac8I	GCNNGC	2 cut(s)	454, 1098
CaiI	CAGNNNCTG	1 cut(s)	121
CfoI	GCGC	1 cut(s)	542
Cfr13I	GGNCC	4 cut(s)	565, 983, 1532, 1565
Cfr42I	CCGCGG	1 cut(s)	283
Csp6I	GTAC	3 cut(s)	81, 1143, 1239
CviAII	CATG	6 cut(s)	134, 569, 1013, 1226, 1236, 1561
CviQI	GTAC	3 cut(s)	81, 1143, 1239
DpnI	GATC	7 cut(s)	57, 198, 258, 600, 635, 689, 1313
DpnII	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
DraIII	CACNNNGTG	1 cut(s)	1579
DrdI	GACNNNNNNGTC	1 cut(s)	1350
DseDI	GACNNNNNNGTC	1 cut(s)	1350
Ecl136II	GAGCTC	1 cut(s)	1333
Eco130I	CCWWGG	1 cut(s)	621
Eco24I	GRGCYC	2 cut(s)	48, 1335
Eco47I	GGWCC	3 cut(s)	565, 1532, 1565
Eco53kI	GAGCTC	1 cut(s)	1333
Eco57I	CTGAAG	1 cut(s)	804
Eco88I	CYCGRG	1 cut(s)	1527
EcoICRI	GAGCTC	1 cut(s)	1333
EcoRII	CCWGG	3 cut(s)	39, 561, 628
EcoT14I	CCWWGG	1 cut(s)	621
EcoT38I	GRGCYC	2 cut(s)	48, 1335
ErhI	CCWWGG	1 cut(s)	621
Esp3I	CGTCTC	1 cut(s)	281
FaeI	CATG	6 cut(s)	137, 572, 1016, 1229, 1239, 1564
FalI	AAGNNNNNCTT	2 cut(s)	425, 457
FaqI	GGGAC	4 cut(s)	115, 314, 523, 1168
FatI	CATG	6 cut(s)	133, 568, 1012, 1225, 1235, 1560
FauNDI	CATATG	1 cut(s)	853
FbaI	TGATCA	1 cut(s)	55
Fnu4HI	GCNGC	4 cut(s)	519, 648, 1233, 1620
FokI	GGATG	4 cut(s)	347, 832, 911, 1205
FriOI	GRGCYC	2 cut(s)	48, 1335
Fsp4HI	GCNGC	4 cut(s)	519, 648, 1233, 1620
FspBI	CTAG	3 cut(s)	480, 936, 1511
GlaI	GCGC	1 cut(s)	541
GluI	GCNGC	4 cut(s)	519, 648, 1233, 1620
GsuI	CTGGAG	1 cut(s)	1428
HaeIII	GGCC	1 cut(s)	985
HhaI	GCGC	1 cut(s)	542
Hin1II	CATG	6 cut(s)	137, 572, 1016, 1229, 1239, 1564
Hin6I	GCGC	1 cut(s)	540
HinP1I	GCGC	1 cut(s)	540
HincII	GTYRAC	1 cut(s)	1063
HindII	GTYRAC	1 cut(s)	1063
HphI	GGTGA	7 cut(s)	115, 199, 812, 854, 926, 1373, 1481
Hpy166II	GTNNAC	4 cut(s)	384, 494, 1063, 1340
Hpy188I	TCNGA	8 cut(s)	12, 55, 64, 72, 201, 378, 1216, 1299
Hpy188III	TCNNGA	7 cut(s)	602, 683, 924, 963, 1315, 1445, 1511
Hpy8I	GTNNAC	4 cut(s)	384, 494, 1063, 1340
HpyAV	CCTTC	3 cut(s)	259, 779, 788
HpyCH4III	ACNGT	6 cut(s)	388, 577, 1186, 1539, 1579, 1603
HpyCH4IV	ACGT	1 cut(s)	837
HpyCH4V	TGCA	7 cut(s)	239, 548, 1016, 1225, 1235, 1325, 1613
HpyF10VI	GCNNNNNNNGC	4 cut(s)	236, 1022, 1241, 1619
HpySE526I	ACGT	1 cut(s)	837
Hsp92II	CATG	6 cut(s)	137, 572, 1016, 1229, 1239, 1564
HspAI	GCGC	1 cut(s)	540
KpnI	GGTACC	1 cut(s)	84
Ksp22I	TGATCA	1 cut(s)	55
KspI	CCGCGG	1 cut(s)	283
Kzo9I	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
LmnI	GCTCC	7 cut(s)	43, 625, 1022, 1306, 1447, 1475, 1525
Lsp1109I	GCAGC	2 cut(s)	659, 1219
LweI	GCATC	3 cut(s)	325, 1034, 1219
MaeI	CTAG	3 cut(s)	480, 936, 1511
MaeII	ACGT	1 cut(s)	837
MaeIII	GTNAC	5 cut(s)	103, 437, 571, 862, 1379
MalI	GATC	7 cut(s)	57, 198, 258, 600, 635, 689, 1313
MboI	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
MboII	GAAGA	3 cut(s)	25, 285, 803
MfeI	CAATTG	3 cut(s)	543, 951, 1017
MflI	RGATCY	3 cut(s)	256, 687, 1311
MhlI	GDGCHC	4 cut(s)	48, 144, 426, 1335
MlyI	GAGTC	3 cut(s)	100, 859, 1150
MmeI	TCCRAC	2 cut(s)	676, 1585
MnlI	CCTC	9 cut(s)	18, 436, 607, 692, 1297, 1402, 1465, 1523, 1536
MseI	TTAA	6 cut(s)	180, 594, 744, 770, 1107, 1293
MslI	CAYNNNNRTG	2 cut(s)	641, 852
MspA1I	CMGCKG	2 cut(s)	282, 1370
MspR9I	CCNGG	3 cut(s)	41, 563, 630
MunI	CAATTG	3 cut(s)	543, 951, 1017
Mva1269I	GAATGC	1 cut(s)	241
MvaI	CCWGG	3 cut(s)	41, 563, 630
MvnI	CGCG	1 cut(s)	282
MwoI	GCNNNNNNNGC	4 cut(s)	236, 1022, 1241, 1619
NdeI	CATATG	1 cut(s)	853
NdeII	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
NlaIII	CATG	6 cut(s)	137, 572, 1016, 1229, 1239, 1564
NlaIV	GGNNCC	6 cut(s)	45, 82, 423, 627, 1521, 1566
NmeAIII	GCCGAG	1 cut(s)	407
NmuCI	GTSAC	3 cut(s)	103, 571, 862
NspI	RCATGY	1 cut(s)	1239
OliI	CACNNNNGTG	1 cut(s)	641
PaeR7I	CTCGAG	1 cut(s)	1527
PaqCI	CACCTGC	1 cut(s)	379
PctI	GAATGC	1 cut(s)	241
PfeI	GAWTC	8 cut(s)	290, 583, 824, 959, 1119, 1273, 1364, 1543
PflFI	GACNNNGTC	1 cut(s)	569
PflMI	CCANNNNNTGG	1 cut(s)	838
PkrI	GCNGC	4 cut(s)	520, 649, 1234, 1621
PleI	GAGTC	3 cut(s)	100, 859, 1150
PpsI	GAGTC	3 cut(s)	100, 859, 1150
Ppu21I	YACGTR	1 cut(s)	838
Psp124BI	GAGCTC	1 cut(s)	1335
Psp6I	CCWGG	3 cut(s)	39, 561, 628
PspGI	CCWGG	3 cut(s)	39, 561, 628
PspN4I	GGNNCC	6 cut(s)	45, 82, 423, 627, 1521, 1566
PspPI	GGNCC	4 cut(s)	565, 983, 1532, 1565
PspXI	VCTCGAGB	1 cut(s)	1527
PstNI	CAGNNNCTG	1 cut(s)	121
PsuI	RGATCY	3 cut(s)	256, 687, 1311
PsyI	GACNNNGTC	1 cut(s)	569
PvuII	CAGCTG	1 cut(s)	1370
RsaI	GTAC	3 cut(s)	82, 1144, 1240
RsaNI	GTAC	3 cut(s)	81, 1143, 1239
RseI	CAYNNNNRTG	2 cut(s)	641, 852
SacI	GAGCTC	1 cut(s)	1335
SacII	CCGCGG	1 cut(s)	283
SaqAI	TTAA	6 cut(s)	180, 594, 744, 770, 1107, 1293
SatI	GCNGC	4 cut(s)	519, 648, 1233, 1620
Sau3AI	GATC	7 cut(s)	55, 196, 256, 598, 633, 687, 1311
Sau96I	GGNCC	4 cut(s)	565, 983, 1532, 1565
SchI	GAGTC	3 cut(s)	100, 859, 1150
ScrFI	CCNGG	3 cut(s)	41, 563, 630
SduI	GDGCHC	4 cut(s)	48, 144, 426, 1335
SfaNI	GCATC	3 cut(s)	325, 1034, 1219
Sfr274I	CTCGAG	1 cut(s)	1527
Sfr303I	CCGCGG	1 cut(s)	283
SgrBI	CCGCGG	1 cut(s)	283
SinI	GGWCC	3 cut(s)	565, 1532, 1565
SlaI	CTCGAG	1 cut(s)	1527
SmiMI	CAYNNNNRTG	2 cut(s)	641, 852
SmlI	CTYRAG	1 cut(s)	1527
SmoI	CTYRAG	1 cut(s)	1527
SsiI	CCGC	3 cut(s)	280, 282, 518
SspMI	CTAG	3 cut(s)	480, 936, 1511
SstI	GAGCTC	1 cut(s)	1335
StyD4I	CCNGG	3 cut(s)	39, 561, 628
StyI	CCWWGG	1 cut(s)	621
TaaI	ACNGT	6 cut(s)	388, 577, 1186, 1539, 1579, 1603
TaiI	ACGT	1 cut(s)	840
TaqI	TCGA	2 cut(s)	1528, 1584
TatI	WGTACW	2 cut(s)	1142, 1238
TauI	GCSGC	1 cut(s)	521
TfiI	GAWTC	8 cut(s)	290, 583, 824, 959, 1119, 1273, 1364, 1543
Tru1I	TTAA	6 cut(s)	180, 594, 744, 770, 1107, 1293
Tru9I	TTAA	6 cut(s)	180, 594, 744, 770, 1107, 1293
TscAI	CASTG	6 cut(s)	30, 310, 787, 867, 1542, 1584
TseFI	GTSAC	3 cut(s)	103, 571, 862
TseI	GCWGC	3 cut(s)	647, 1232, 1619
Tsp45I	GTSAC	3 cut(s)	103, 571, 862
TspDTI	ATGAA	2 cut(s)	750, 1549
TspRI	CASTG	6 cut(s)	30, 310, 787, 867, 1542, 1584
Tth111I	GACNNNGTC	1 cut(s)	569
Van91I	CCANNNNNTGG	1 cut(s)	838
VpaK11BI	GGWCC	3 cut(s)	565, 1532, 1565
XapI	RAATTY	1 cut(s)	65
XbaI	TCTAGA	1 cut(s)	1510
XceI	RCATGY	1 cut(s)	1239
XcmI	CCANNNNNNNNNTGG	3 cut(s)	160, 533, 575
XhoI	CTCGAG	1 cut(s)	1527
XspI	CTAG	3 cut(s)	480, 936, 1511

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.