Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0001862

Physical Location & Seq

Genomic Coordinates Reverse (-)

12633818 .. 12644110

Gene Structure 10293 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00090980.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3132 bp

ATGGCCAAAGGAGGTGGACTCTCCATTGATTCAGATCCAATTGGCTACTTGTCCCTCCACAAACCCATAGTCCTCAACTCCTTTCAAGATCAACACCCTAATTCTAGTTCTACTACCCACAAACAACACCTCTCTGGGTTTTCAATGGATCATCACACCCACCAAGCCAGGTCACCTCCACCGCCCCAGACTCTGCAATTCTCAGTCAACCTCAACTGCACCGACGATAACGACGACCGAGAAAACTCGCCGCCGCAATCGGACGACAACCAGCGCAAGGTCGTAGACGAGAGGGACTTCTTTGCTGATAACAACAACAAGGCCGAGGAAGCTTCCACTGAGAAAATGGACTTCGACGATGGCCCTTCCGGCATGGAATTCAACGTAAACACTGGGTTAAATCTTCTCATCACAAACACTAGTAGTGACCAGTCAGTTGTGGATGATAGCATTTCGTCGAGCATGGAGGATAAAAGAGCCAAAAGTGAGCTGGCTGTTCTTCAAGCTGAGCTGGAGAGGATGAACGCGGAGAATCAGAGGCTGAGAGAGATGCTTAATCAGGTCACTACCAATTACAGTGCTCTACAAGTGCATTTGCTGAGTTTGATGCAAAACCAGAAGGCTGAGAACAGTGTGGAAGTGGAAGAGAAAAAGCTTAATAATGGCAGTGGATCGCAGATGGTGGTTCCGAGGCAATTTATGGATCTTGGTTTGGCTGCAAATGGGGATGCCGAAGAGCCATCACATTCTTCTTCGGATGAGAGAAGCCGGGAACGGTCTGGATCAGGAGATAATGGAAAGGTTGGTGCTGGGAATGTTGGTGATGAGGGGCCTGCGTTTGAACAGGAGAAGAAGGAATTTGGTCGGGGGATTAGTGGGAGAGAGGAGAGTCCTGATCAACAAACGCAAAGTTGGGGACCTAACAAAGTTCCGAGACTCAATTCTCCAAAGGAGGTTGATCAAACTGAGGCTACAATGAGAAAGGCTCGGGTTTCTGTTAGAGCTCGATCAGAGGCACCCATGATCACTGATGGATGTCAATGGCGTAAGTACGGGCAAAAGATGGCGAAAGGAAACCCATGTCCTCGAGCTTATTATAGATGCACCATGGCTGCTGGTTGCCCGGTTCGAAAACAAGTACAACGATGTGCTGAAGATAGGACAATCCTCATCACAACATATGAAGGCAATCACAACCACCCATTGCCACCAGCCGCCATGGCAATGGCGTCGACCACTTCATCTGCAGCCCGGATGCTGCTTTCCGGGTCTATGCCAAGCGCGGATGGCCTAATGGACTCAAACTTCCTCACTAGGACCATGCTCCCATGCTCCTCAAGCATGGCCACAATCTCAGCCTCAGCTCCATTCCCCACTGTTACATTGGATTTAACACAGTCCCCAAACCCTTTACAGTTCCAAAGGCCACCAGGCCAATTCAACATCCCATTCCCAAACCCGTCTCAGAACTTGACCAATGGACCTGTCTCATTGCTGCCCCAAATCTTCAGTCAGGCGCTCTACAACCAGTCCAAGTTTTCTGGCCTTCAAATGTCACAGGACATGGACCTGTTCGTCTTCTCCCAGGAGCCCAACTCTGATCACTCAGAATTTCTGAAAATGGGTACCAAAATTAGGGCTTGTTTGGTGACTCTATTTCAGTTTCTGCTTCTGTCCCATGTGGTGCTCTCCACACCCAATGATGGGTTTGTTAGAATTGGACTTAAAAAGGTGAAATTGGATCAGACATCTCAGCTCCATAGGGAGCCTAATGCTAAGAATGCACAAGGAAGTATGAGAGATCCTTTGAAGGAGTATCGTCTCCGCGGTGGGGATTCTTCTGGGGACACTGATATTGTTGAACTGAAGAACTATATGGATGCTCAGTATTTTGGGGAGATAGGTATTGGCACGCCTGCACAGAAGTTTACTGTGATATTTGATACTGGAAGTTCTAACCTTTGGGTGCCCTCGGCAAAGTGTTACTTCTCGGTGGCTTGCTTTTTTCACTCCAAGGACATCTGCGGCTATCCAGTACGGAACTGGCGCAATTGCAGGTTTTTTAGCCAGGACCATGTCACAGTTGGGAATCTTGTTGTTAAGGATCAAGATTTTATTGAGGCAACCAAGGAGCCTGGGATCACATTTGTGGCAGCTAAGTTTGATGGCATACTTGGACTTGGATTTCAAGAAATCTCAGTTGGAGGTGCTGTTCCTGTCTGGTATAATTTGGTCAATCAAGGTCTTGTTAAAGAACCAGTATTTTCATTTTGGTTAAATCGCAACACTGAAGGGGAAGAAGGGGGTGAGATTGTATTTGGTGGGATGGATTCTAACCATTACGTGGGTGAGCATACATATGTTCCAGTGACTCAAAAAGGCTATTGGCAGTTTGATATGGGGGATGTGCTAATTGGTGGTGAAACATCTGGACTTTGTGCTAGTGGTTGTTCAGCAATTGCTGATTCGGGAACTTCCCTATTGGCAGGGCCAACGACCATTATCACTCAAATAAATCATGCAATTGGAGCATCTGGTATTGTAAGCCAAGAGTGTAAGGCAGTTGTTGACCAGTATGGGAAAATTATACTGGAATTGTTACTGGCTCAGGTTAAACCCCAAAGAATCTGCTCTCAAATTGGTGTATGTACTTTTGACGGGACTCGTGGTGTGAGTATGGGCATTGAAACTGTGGTGGATGAGAATATGGAAACTACATCTGATGGCGTGCATGATGCTGCATGTACTGCTTGTGAGATGGCAGTAGTATGGATACAGAATCGGCTAAAGCTAAATCTTAATCAGACAGAGGAGCAGATCTTGAACTATGCAAATGAGCTCTGTGAACGACTGCCCAGTCCAAGTGGTGAATCAGCTGTTCAATGTAACGCTCTCTCCTCCTTGCCCATTATATCATTTACCATCGGTGGTAAAGTATTTGACCTTGCTCCAGAGCAGTACGTTCTCAAAGTGGGTGAGGGAGCTACGGCACAATGCCTTAGTGGATTTATAGCTCTAGATGTGGCTCCACCTCGAGGACCACTGTGGATTCTGGGAGATATTTTCATGGGTCAGTATCACACAGTGTTCGACTATGGGAAAGCTACTGTTGGATTTGCAGAAGCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

1043

Amino Acids

113.9

Weight (kDa)

5.12

Isoelectric Point (pI)

41.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	344 - 401	2.4e-25	WRKY DNA -binding domain
Asp	PF00026	629 - 671	3.3e-16	Eukaryotic aspartyl protease
Asp	PF00026	687 - 1042	5.1e-78	Eukaryotic aspartyl protease
SapB_2	PF03489	850 - 883	1.2e-11	Saposin-like type B, region 2
SapB_1	PF05184	914 - 952	7.8e-09	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11910 AT1G11910 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290
fragaria_vesca	FvH4_3g23140 FvH4_3g23140 FvH4_6g42880 FvH4_6g42880
malus_domestica	MD00G1159300.v1.1 MD09G1111100.v1.1 MD11G1213600.v1.1 MD15G1413400.v1.1 MD17G1098900.v1.1 MD17G1282600.v1.1 MD17G1282700.v1.1
prunus_persica	Prupe.3G214900_v2.0.a1 Prupe.3G214900_v2.0.a1 Prupe.4G217700_v2.0.a1
pyrus_communis	pycom03g14920 pycom09g03360 pycom11g18820 pycom17g09430
rosa_chinensis	RchiOBHm_Chr2g0158811 RchiOBHm_Chr5g0040781
rosa_laevigata	RLG00000021115 RLG00000034001
rosa_multiflora	Rmu_sc0001579.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039910 Rroxscaffold_2G00090980
rosa_rugosa	Rorug02G0472700 Rorug05G0187200 Rorug05G0187300
rosa_samantha	Rh2AG538500 Rh2BG551200 Rh2CG521800 Rh2DG561000 Rh5AG273000 Rh5BG278100 Rh5CG310100 Rh5DG286000
rosa_wichuraiana	Rw2G044570 Rw5G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1574, 2856
AbsI	CCTCGAGG	1 cut(s)	3033
Acc36I	ACCTGC	1 cut(s)	2046
Acc65I	GGTACC	1 cut(s)	1625
AccB1I	GGYRCC	3 cut(s)	1015, 1625, 1966
AccB7I	CCANNNNNTGG	1 cut(s)	2344
AccI	GTMKAC	2 cut(s)	285, 1232
AccII	CGCG	3 cut(s)	527, 1283, 1827
AciI	CCGC	9 cut(s)	182, 251, 254, 527, 1215, 1283, 1825, 1827, 2025
AclWI	GGATC	9 cut(s)	29, 156, 679, 711, 790, 1749, 1796, 2112, 2147
AcoI	YGGCCR	2 cut(s)	3, 1344
AcsI	RAATTY	3 cut(s)	377, 857, 1610
AcuI	CTGAAG	4 cut(s)	1173, 1492, 1886, 2310
AcyI	GRCGYC	1 cut(s)	1229
AdeI	CACNNNGTG	1 cut(s)	3085
AfaI	GTAC	7 cut(s)	1052, 1140, 1627, 2037, 2650, 2746, 2960
AfiI	CCNNNNNNNGG	7 cut(s)	277, 1635, 1703, 1704, 1831, 2344, 3035
AhlI	ACTAGT	1 cut(s)	419
AjnI	CCWGG	5 cut(s)	167, 1429, 1584, 2067, 2134
AjuI	GAANNNNNNNTTGG	2 cut(s)	2184, 2216
AleI	CACNNNNGTG	1 cut(s)	2147
AloI	GAACNNNNNNTCC	2 cut(s)	2196, 2228
Alw21I	GWGCWC	4 cut(s)	583, 1006, 1689, 2841
Alw26I	GTCTC	4 cut(s)	928, 1467, 1492, 1826
AlwI	GGATC	9 cut(s)	29, 156, 679, 711, 790, 1749, 1796, 2112, 2147
AlwNI	CAGNNNCTG	3 cut(s)	193, 541, 1666
Ama87I	CYCGRG	3 cut(s)	987, 1086, 3033
ApeKI	GCWGC	8 cut(s)	716, 1112, 1247, 1258, 1495, 2153, 2738, 3125
ApoI	RAATTY	3 cut(s)	377, 857, 1610
ArsI	GACNNNNNNTTYG	4 cut(s)	54, 86, 1495, 1527
Asp718I	GGTACC	1 cut(s)	1625
AspLEI	GCGC	4 cut(s)	276, 1283, 1519, 2049
AspS9I	GGNCC	9 cut(s)	362, 830, 917, 1317, 1481, 1567, 2071, 2489, 3038
AsuC2I	CCSGG	4 cut(s)	770, 1124, 1252, 1267
AsuHPI	GGTGA	9 cut(s)	165, 833, 1660, 1744, 2318, 2360, 2432, 2879, 2987
AsuII	TTCGAA	1 cut(s)	1129
AvaI	CYCGRG	3 cut(s)	987, 1086, 3033
AvaII	GGWCC	6 cut(s)	917, 1317, 1481, 1567, 2071, 3038
BaeGI	GKGCMC	1 cut(s)	1971
BalI	TGGCCA	2 cut(s)	5, 1346
BanI	GGYRCC	3 cut(s)	1015, 1625, 1966
BanII	GRGCYC	3 cut(s)	1006, 1593, 2841
BauI	CACGAG	1 cut(s)	2664
BbsI	GAAGAC	1 cut(s)	1570
Bbv12I	GWGCWC	4 cut(s)	583, 1006, 1689, 2841
BbvCI	CCTCAGC	1 cut(s)	1360
BbvI	GCAGC	7 cut(s)	703, 1099, 1245, 1259, 1482, 2165, 2725
BceAI	ACGGC	1 cut(s)	3003
BcgI	CGANNNNNNTGC	2 cut(s)	1212, 1246
BciT130I	CCWGG	5 cut(s)	169, 1431, 1586, 2069, 2136
BciVI	GTATCC	1 cut(s)	2766
BclI	TGATCA	4 cut(s)	895, 958, 1023, 1600
BcnI	CCSGG	4 cut(s)	770, 1124, 1252, 1267
BcoDI	GTCTC	4 cut(s)	928, 1467, 1492, 1826
BcuI	ACTAGT	1 cut(s)	419
BfaI	CTAG	5 cut(s)	105, 420, 1314, 2442, 3017
BfmI	CTRYAG	1 cut(s)	1245
BfoI	RGCGCY	1 cut(s)	1520
BfuAI	ACCTGC	1 cut(s)	2046
BfuI	GTATCC	1 cut(s)	2766
BglI	GCCNNNNNGGC	2 cut(s)	369, 1220
BglII	AGATCT	1 cut(s)	2817
BlpI	GCTNAGC	1 cut(s)	507
Bme1390I	CCNGG	9 cut(s)	169, 770, 1124, 1252, 1267, 1431, 1586, 2069, 2136
Bme18I	GGWCC	6 cut(s)	917, 1317, 1481, 1567, 2071, 3038
BmeT110I	CYCGRG	3 cut(s)	987, 1086, 3033
BmgT120I	GGNCC	9 cut(s)	362, 830, 917, 1317, 1481, 1567, 2071, 2489, 3038
BmrFI	CCNGG	9 cut(s)	169, 770, 1124, 1252, 1267, 1431, 1586, 2069, 2136
BmrI	ACTGGG	2 cut(s)	402, 2850
BmsI	GCATC	8 cut(s)	540, 597, 718, 1091, 1245, 1870, 2540, 2725
BmuI	ACTGGG	2 cut(s)	402, 2850
BpiI	GAAGAC	1 cut(s)	1570
BplI	GAGNNNNNCTC	5 cut(s)	35, 970, 1002, 1580, 1612
BpmI	CTGGAG	2 cut(s)	533, 2934
Bpu10I	CCTNAGC	2 cut(s)	1360, 2607
Bpu1102I	GCTNAGC	1 cut(s)	507
Bpu14I	TTCGAA	1 cut(s)	1129
BpuEI	CTTGAG	1 cut(s)	1321
BpuMI	CCSGG	4 cut(s)	770, 1124, 1252, 1267
BsaAI	YACGTR	1 cut(s)	2344
BsaBI	GATNNNNATC	1 cut(s)	33
BsaHI	GRCGYC	1 cut(s)	1229
BsaXI	ACNNNNNCTCC	4 cut(s)	2196, 2226, 2879, 2909
Bsc4I	CCNNNNNNNGG	7 cut(s)	277, 1635, 1703, 1704, 1831, 2344, 3035
Bse3DI	GCAATG	3 cut(s)	1202, 1230, 1490
Bse8I	GATNNNNATC	1 cut(s)	33
BseBI	CCWGG	5 cut(s)	169, 1431, 1586, 2069, 2136
BseJI	GATNNNNATC	1 cut(s)	33
BseLI	CCNNNNNNNGG	7 cut(s)	277, 1635, 1703, 1704, 1831, 2344, 3035
BseMI	GCAATG	3 cut(s)	1202, 1230, 1490
BseRI	GAGGAG	4 cut(s)	899, 1324, 2825, 2887
BseSI	GKGCMC	1 cut(s)	1971
BseXI	GCAGC	7 cut(s)	703, 1099, 1245, 1259, 1482, 2165, 2725
BseYI	CCCAGC	1 cut(s)	809
BsgI	GTGCAG	2 cut(s)	202, 1902
Bsh1236I	CGCG	3 cut(s)	527, 1283, 1827
Bsh1285I	CGRYCG	1 cut(s)	238
BshNI	GGYRCC	3 cut(s)	1015, 1625, 1966
BsiEI	CGRYCG	1 cut(s)	238
BsiHKAI	GWGCWC	4 cut(s)	583, 1006, 1689, 2841
BsiHKCI	CYCGRG	3 cut(s)	987, 1086, 3033
BsiSI	CCGG	5 cut(s)	369, 769, 1124, 1252, 1266
BslFI	GGGAC	7 cut(s)	37, 308, 930, 1384, 1660, 1859, 2674
BslI	CCNNNNNNNGG	7 cut(s)	277, 1635, 1703, 1704, 1831, 2344, 3035
BsmAI	GTCTC	4 cut(s)	928, 1467, 1492, 1826
BsmBI	CGTCTC	2 cut(s)	1467, 1826
BsmFI	GGGAC	7 cut(s)	37, 308, 930, 1384, 1660, 1859, 2674
BsmI	GAATGC	1 cut(s)	1786
BsoBI	CYCGRG	3 cut(s)	987, 1086, 3033
Bsp119I	TTCGAA	1 cut(s)	1129
Bsp1286I	GDGCHC	6 cut(s)	583, 1006, 1593, 1689, 1971, 2841
Bsp1720I	GCTNAGC	1 cut(s)	507
Bsp19I	CCATGG	2 cut(s)	1107, 1218
BspACI	CCGC	9 cut(s)	182, 251, 254, 527, 1215, 1283, 1825, 1827, 2025
BspFNI	CGCG	3 cut(s)	527, 1283, 1827
BspMAI	CTGCAG	1 cut(s)	1249
BspMI	ACCTGC	1 cut(s)	2046
BspPI	GGATC	9 cut(s)	29, 156, 679, 711, 790, 1749, 1796, 2112, 2147
BspQI	GCTCTTC	1 cut(s)	729
BspT104I	TTCGAA	1 cut(s)	1129
BspT107I	GGYRCC	3 cut(s)	1015, 1625, 1966
BsrDI	GCAATG	3 cut(s)	1202, 1230, 1490
BssNI	GRCGYC	1 cut(s)	1229
BssSI	CACGAG	1 cut(s)	2664
BssT1I	CCWWGG	4 cut(s)	1107, 1218, 2013, 2127
Bst2BI	CACGAG	1 cut(s)	2664
Bst2UI	CCWGG	5 cut(s)	169, 1431, 1586, 2069, 2136
Bst6I	CTCTTC	2 cut(s)	639, 729
BstACI	GRCGYC	1 cut(s)	1229
BstAPI	GCANNNNNTGC	1 cut(s)	2747
BstBAI	YACGTR	1 cut(s)	2344
BstBI	TTCGAA	1 cut(s)	1129
BstC8I	GCNNGC	6 cut(s)	492, 834, 1913, 1917, 1999, 2729
BstDSI	CCRYGG	3 cut(s)	1107, 1218, 1825
BstEII	GGTNACC	1 cut(s)	171
BstFNI	CGCG	3 cut(s)	527, 1283, 1827
BstH2I	RGCGCY	1 cut(s)	1520
BstHHI	GCGC	4 cut(s)	276, 1283, 1519, 2049
BstMAI	GTCTC	4 cut(s)	928, 1467, 1492, 1826
BstMCI	CGRYCG	1 cut(s)	238
BstMWI	GCNNNNNNNGC	9 cut(s)	329, 369, 1220, 1287, 1338, 1781, 2528, 2747, 3125
BstNI	CCWGG	5 cut(s)	169, 1431, 1586, 2069, 2136
BstNSI	RCATGY	1 cut(s)	2745
BstPI	GGTNACC	1 cut(s)	171
BstSCI	CCNGG	9 cut(s)	167, 768, 1122, 1250, 1265, 1429, 1584, 2067, 2134
BstSFI	CTRYAG	1 cut(s)	1245
BstSLI	GKGCMC	1 cut(s)	1971
BstUI	CGCG	3 cut(s)	527, 1283, 1827
BstV1I	GCAGC	7 cut(s)	703, 1099, 1245, 1259, 1482, 2165, 2725
BstV2I	GAAGAC	1 cut(s)	1570
BstX2I	RGATCY	4 cut(s)	34, 703, 1801, 2817
BstXI	CCANNNNNNTGG	1 cut(s)	1225
BstYI	RGATCY	4 cut(s)	34, 703, 1801, 2817
BsuI	GTATCC	1 cut(s)	2766
BtgI	CCRYGG	3 cut(s)	1107, 1218, 1825
BtsI	GCAGTG	1 cut(s)	673
BveI	ACCTGC	1 cut(s)	2046
Cac8I	GCNNGC	6 cut(s)	492, 834, 1913, 1917, 1999, 2729
CaiI	CAGNNNCTG	3 cut(s)	193, 541, 1666
CfoI	GCGC	4 cut(s)	276, 1283, 1519, 2049
Cfr13I	GGNCC	9 cut(s)	362, 830, 917, 1317, 1481, 1567, 2071, 2489, 3038
Cfr42I	CCGCGG	1 cut(s)	1828
CseI	GACGC	1 cut(s)	1218
Csp6I	GTAC	7 cut(s)	1051, 1139, 1626, 2036, 2649, 2745, 2959
CspCI	CAANNNNNGTGG	1 cut(s)	30
CviQI	GTAC	7 cut(s)	1051, 1139, 1626, 2036, 2649, 2745, 2959
DraIII	CACNNNGTG	1 cut(s)	3085
DrdI	GACNNNNNNGTC	2 cut(s)	1574, 2856
DseDI	GACNNNNNNGTC	2 cut(s)	1574, 2856
EaeI	YGGCCR	2 cut(s)	3, 1344
Eam1104I	CTCTTC	2 cut(s)	639, 729
EarI	CTCTTC	2 cut(s)	639, 729
Ecl136II	GAGCTC	2 cut(s)	1004, 2839
Eco130I	CCWWGG	4 cut(s)	1107, 1218, 2013, 2127
Eco24I	GRGCYC	3 cut(s)	1006, 1593, 2841
Eco47I	GGWCC	6 cut(s)	917, 1317, 1481, 1567, 2071, 3038
Eco53kI	GAGCTC	2 cut(s)	1004, 2839
Eco57I	CTGAAG	4 cut(s)	1173, 1492, 1886, 2310
Eco88I	CYCGRG	3 cut(s)	987, 1086, 3033
Eco91I	GGTNACC	1 cut(s)	171
EcoICRI	GAGCTC	2 cut(s)	1004, 2839
EcoO109I	RGGNCCY	2 cut(s)	830, 917
EcoO65I	GGTNACC	1 cut(s)	171
EcoRI	GAATTC	1 cut(s)	377
EcoRII	CCWGG	5 cut(s)	167, 1429, 1584, 2067, 2134
EcoT14I	CCWWGG	4 cut(s)	1107, 1218, 2013, 2127
EcoT38I	GRGCYC	3 cut(s)	1006, 1593, 2841
ErhI	CCWWGG	4 cut(s)	1107, 1218, 2013, 2127
Esp3I	CGTCTC	2 cut(s)	1467, 1826
FalI	AAGNNNNNCTT	2 cut(s)	1970, 2002
FaqI	GGGAC	7 cut(s)	37, 308, 930, 1384, 1660, 1859, 2674
FauNDI	CATATG	2 cut(s)	1180, 2359
FbaI	TGATCA	4 cut(s)	895, 958, 1023, 1600
FblI	GTMKAC	2 cut(s)	285, 1232
FriOI	GRGCYC	3 cut(s)	1006, 1593, 2841
FspBI	CTAG	5 cut(s)	105, 420, 1314, 2442, 3017
GlaI	GCGC	4 cut(s)	275, 1282, 1518, 2048
GsaI	CCCAGC	1 cut(s)	813
GsuI	CTGGAG	2 cut(s)	533, 2934
HaeII	RGCGCY	1 cut(s)	1520
HapII	CCGG	5 cut(s)	369, 769, 1124, 1252, 1266
HgaI	GACGC	1 cut(s)	1218
HhaI	GCGC	4 cut(s)	276, 1283, 1519, 2049
Hin1I	GRCGYC	1 cut(s)	1229
Hin6I	GCGC	4 cut(s)	274, 1281, 1517, 2047
HinP1I	GCGC	4 cut(s)	274, 1281, 1517, 2047
HincII	GTYRAC	3 cut(s)	208, 1233, 2569
HindII	GTYRAC	3 cut(s)	208, 1233, 2569
HindIII	AAGCTT	2 cut(s)	330, 653
HpaII	CCGG	5 cut(s)	369, 769, 1124, 1252, 1266
HphI	GGTGA	9 cut(s)	165, 833, 1660, 1744, 2318, 2360, 2432, 2879, 2987
Hpy166II	GTNNAC	8 cut(s)	17, 208, 286, 388, 1233, 1929, 2569, 2846
Hpy8I	GTNNAC	8 cut(s)	17, 208, 286, 388, 1233, 1929, 2569, 2846
Hpy99I	CGWCG	5 cut(s)	227, 236, 359, 460, 1234
HpyAV	CCTTC	8 cut(s)	375, 613, 847, 1178, 1556, 1804, 2285, 2294
HpyCH4IV	ACGT	3 cut(s)	384, 2343, 2961
HpyF10VI	GCNNNNNNNGC	9 cut(s)	329, 369, 1220, 1287, 1338, 1781, 2528, 2747, 3125
HpySE526I	ACGT	3 cut(s)	384, 2343, 2961
Hsp92I	GRCGYC	1 cut(s)	1229
HspAI	GCGC	4 cut(s)	274, 1281, 1517, 2047
KpnI	GGTACC	1 cut(s)	1629
Ksp22I	TGATCA	4 cut(s)	895, 958, 1023, 1600
KspI	CCGCGG	1 cut(s)	1828
LguI	GCTCTTC	1 cut(s)	729
Lsp1109I	GCAGC	7 cut(s)	703, 1099, 1245, 1259, 1482, 2165, 2725
LweI	GCATC	8 cut(s)	540, 597, 718, 1091, 1245, 1870, 2540, 2725
MaeI	CTAG	5 cut(s)	105, 420, 1314, 2442, 3017
MaeII	ACGT	3 cut(s)	384, 2343, 2961
MfeI	CAATTG	4 cut(s)	39, 2050, 2457, 2523
MflI	RGATCY	4 cut(s)	34, 703, 1801, 2817
MhlI	GDGCHC	6 cut(s)	583, 1006, 1593, 1689, 1971, 2841
MlsI	TGGCCA	2 cut(s)	5, 1346
MluNI	TGGCCA	2 cut(s)	5, 1346
MlyI	GAGTC	8 cut(s)	12, 184, 898, 930, 1292, 1645, 2365, 2656
MmeI	TCCRAC	2 cut(s)	2182, 3091
Mox20I	TGGCCA	2 cut(s)	5, 1346
MscI	TGGCCA	2 cut(s)	5, 1346
MslI	CAYNNNNRTG	3 cut(s)	1223, 2147, 2358
Msp20I	TGGCCA	2 cut(s)	5, 1346
MspA1I	CMGCKG	2 cut(s)	1827, 2876
MspI	CCGG	5 cut(s)	369, 769, 1124, 1252, 1266
MspR9I	CCNGG	9 cut(s)	169, 770, 1124, 1252, 1267, 1431, 1586, 2069, 2136
MunI	CAATTG	4 cut(s)	39, 2050, 2457, 2523
Mva1269I	GAATGC	1 cut(s)	1786
MvaI	CCWGG	5 cut(s)	169, 1431, 1586, 2069, 2136
MvnI	CGCG	3 cut(s)	527, 1283, 1827
MwoI	GCNNNNNNNGC	9 cut(s)	329, 369, 1220, 1287, 1338, 1781, 2528, 2747, 3125
NciI	CCSGG	4 cut(s)	770, 1124, 1252, 1267
NcoI	CCATGG	2 cut(s)	1107, 1218
NdeI	CATATG	2 cut(s)	1180, 2359
NmeAIII	GCCGAG	2 cut(s)	349, 1952
NmuCI	GTSAC	7 cut(s)	171, 425, 562, 1554, 1648, 2077, 2368
NspI	RCATGY	1 cut(s)	2745
NspV	TTCGAA	1 cut(s)	1129
OliI	CACNNNNGTG	1 cut(s)	2147
PaeR7I	CTCGAG	2 cut(s)	1086, 3033
PciSI	GCTCTTC	1 cut(s)	729
PcsI	WCGNNNNNNNCGW	1 cut(s)	231
PctI	GAATGC	1 cut(s)	1786
PflFI	GACNNNGTC	1 cut(s)	2075
PflMI	CCANNNNNTGG	1 cut(s)	2344
PleI	GAGTC	8 cut(s)	12, 184, 897, 930, 1292, 1645, 2365, 2656
PpsI	GAGTC	8 cut(s)	12, 184, 897, 930, 1292, 1645, 2365, 2656
Ppu21I	YACGTR	1 cut(s)	2344
PpuMI	RGGWCCY	1 cut(s)	917
Psp124BI	GAGCTC	2 cut(s)	1006, 2841
Psp5II	RGGWCCY	1 cut(s)	917
Psp6I	CCWGG	5 cut(s)	167, 1429, 1584, 2067, 2134
PspEI	GGTNACC	1 cut(s)	171
PspFI	CCCAGC	1 cut(s)	809
PspGI	CCWGG	5 cut(s)	167, 1429, 1584, 2067, 2134
PspPI	GGNCC	9 cut(s)	362, 830, 917, 1317, 1481, 1567, 2071, 2489, 3038
PspPPI	RGGWCCY	1 cut(s)	917
PspXI	VCTCGAGB	2 cut(s)	1086, 3033
PstI	CTGCAG	1 cut(s)	1249
PstNI	CAGNNNCTG	3 cut(s)	193, 541, 1666
PsuI	RGATCY	4 cut(s)	34, 703, 1801, 2817
PsyI	GACNNNGTC	1 cut(s)	2075
PvuII	CAGCTG	1 cut(s)	2876
RsaI	GTAC	7 cut(s)	1052, 1140, 1627, 2037, 2650, 2746, 2960
RsaNI	GTAC	7 cut(s)	1051, 1139, 1626, 2036, 2649, 2745, 2959
RseI	CAYNNNNRTG	3 cut(s)	1223, 2147, 2358
SacI	GAGCTC	2 cut(s)	1006, 2841
SacII	CCGCGG	1 cut(s)	1828
SalI	GTCGAC	1 cut(s)	1231
SapI	GCTCTTC	1 cut(s)	729
Sau96I	GGNCC	9 cut(s)	362, 830, 917, 1317, 1481, 1567, 2071, 2489, 3038
SchI	GAGTC	8 cut(s)	12, 184, 898, 930, 1292, 1645, 2365, 2656
ScrFI	CCNGG	9 cut(s)	169, 770, 1124, 1252, 1267, 1431, 1586, 2069, 2136
SduI	GDGCHC	6 cut(s)	583, 1006, 1593, 1689, 1971, 2841
SfaNI	GCATC	8 cut(s)	540, 597, 718, 1091, 1245, 1870, 2540, 2725
SfcI	CTRYAG	1 cut(s)	1245
Sfr274I	CTCGAG	2 cut(s)	1086, 3033
Sfr303I	CCGCGG	1 cut(s)	1828
SfuI	TTCGAA	1 cut(s)	1129
SgrBI	CCGCGG	1 cut(s)	1828
SinI	GGWCC	6 cut(s)	917, 1317, 1481, 1567, 2071, 3038
SlaI	CTCGAG	2 cut(s)	1086, 3033
SmiMI	CAYNNNNRTG	3 cut(s)	1223, 2147, 2358
SmlI	CTYRAG	3 cut(s)	1086, 1336, 3033
SmoI	CTYRAG	3 cut(s)	1086, 1336, 3033
SpeI	ACTAGT	1 cut(s)	419
SsiI	CCGC	9 cut(s)	182, 251, 254, 527, 1215, 1283, 1825, 1827, 2025
SspMI	CTAG	5 cut(s)	105, 420, 1314, 2442, 3017
SstI	GAGCTC	2 cut(s)	1006, 2841
StyD4I	CCNGG	9 cut(s)	167, 768, 1122, 1250, 1265, 1429, 1584, 2067, 2134
StyI	CCWWGG	4 cut(s)	1107, 1218, 2013, 2127
TaiI	ACGT	3 cut(s)	387, 2346, 2964
TaqI	TCGA	8 cut(s)	354, 458, 1006, 1087, 1129, 1232, 3034, 3090
TaqII	GACCGA	1 cut(s)	252
TatI	WGTACW	3 cut(s)	1138, 2648, 2744
TauI	GCSGC	4 cut(s)	253, 256, 1217, 2028
TseFI	GTSAC	7 cut(s)	171, 425, 562, 1554, 1648, 2077, 2368
TseI	GCWGC	8 cut(s)	716, 1112, 1247, 1258, 1495, 2153, 2738, 3125
Tsp45I	GTSAC	7 cut(s)	171, 425, 562, 1554, 1648, 2077, 2368
TspDTI	ATGAA	5 cut(s)	536, 1197, 1230, 2256, 3055
TspGWI	ACGGA	1 cut(s)	2053
Tth111I	GACNNNGTC	1 cut(s)	2075
Van91I	CCANNNNNTGG	1 cut(s)	2344
VpaK11BI	GGWCC	6 cut(s)	917, 1317, 1481, 1567, 2071, 3038
XapI	RAATTY	3 cut(s)	377, 857, 1610
XbaI	TCTAGA	1 cut(s)	3016
XceI	RCATGY	1 cut(s)	2745
XcmI	CCANNNNNNNNNTGG	5 cut(s)	343, 487, 1381, 2040, 2081
XhoI	CTCGAG	2 cut(s)	1086, 3033
XmiI	GTMKAC	2 cut(s)	285, 1232
XspI	CTAG	5 cut(s)	105, 420, 1314, 2442, 3017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.