Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001862

Physical Location & Seq

Genomic Coordinates Forward (+)

71210384 .. 71215722

Gene Structure 5339 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G044570.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGGGTACCAAAATTAGGGTGACTCTATTTCAGTTTCTGCTTCTGTCCCATGTGGTGCTCTCCACACCCAATGATGGGTTGGTTAGAATTGGACTTAAAAAGGTGAAATTGGATCAGACATCTCAGCTTCACAGGGAGACTAATGCTAAGAATGCACAAGGAAGTATGAGAGATCCTTTGAAGGAGTATCGTCTCCGCGGTGGGGATTCTTCTGGGGACACTGATATTGTTGAACTGAAGAACTATATGGATGCTCAGTATTTTGGGGAGATTGGTATTGGCACACCTGCTCAGAAGTTTACTGTGATATTTGATACTGGAAGTTCTAACCTTTGGGTGCCCTCGGCAAAGTGTTACTTCTCGGTGGCTTGCTTTTTTCACGCCAAGTATAAGTCTAGTCAGTCAAGTTCACACACAAAGAATGGGACATCTGCGGCTATCCAGTACGGAACTGGCGCAATTGCAGGTTTTTTTAGCCAGGACCATGTCACAGTTGGGAATCTTGTTGTCAAGGATCAAGATTTTATTGAGGCAACCAAGGAGCCTGGGATCACATTTGTGGCAGCTAAGTTTGATGGTATACTTGGACTTGGATTTCAAGAGATCTCAGTTGGAGGTGCTGTTCCTGTCTGGTATAATTTGGTCAATCAAGGTCTTGTTAAAGAACCAGTATTTTCATTTTGGTTAAATCGCAACACTGAAGGGGAAGAAGGGGGTGAGATTGTATTTGGTGGGATGGATTCTAACCATTACGTGGGTGAGCATACATATGTTCCAGTGACTCAAAAAGGCTATTGGCAGTTTGATATGGGGGATGTGCTAATTGGTGGTGAAACATCTGGACTTTGTGCTAGTGGTTGTTCAGCAATTGCTGATTCAGGAACTTCCCTATTGGCAGGGCCAACGACCATTATCACTCAAATAAATCATGCAATTGGAGCATCTGGTATTGTAAGCCAAGAGTGTAAGGCAGTTGTTGACCAGTATGGGAAAATTATACTGGAATTGTTGCTGGCTCAGGTTAAACCCCAAAGAATCTGCTCTCAAATTGGTGTATGTACTTTTGACGGGACTCGTGGTGTGAGTATGGGCATTGAAACTGTGGTGGATGAGAATATGGAAACTACATCTGATGGTGTGCATGATGCTGCATGTACTGCTTGTGAGATGGCAGTAGTATGGATACAGAATCAGCTAAAGCTAAATCTTAATCAGACAGAGGAGCAGATCTTGAACTATGCAAATGAGCTCTGTGAACGACTGCCCAGTCCAAGTGGTGAATCAGCTGTTCAATGTAACGCTCTCTCCTCCTTGCCCAGTATATCATTTACCATCGGTGGTAAAGTATTTGACCTTGCTCCAGAGCAGTACGTTCTCAAAGTGGGTGAGGGAGCTACGGCACAATGCCTTAGTGGATTTATAGCTCTAGATGTGGCTCCACCTCGAGGACCACTGTGGATTCTGGGAGATATTTTCATGGGTCAGTATCACACAGTGTTCGACTATGGGAAAGCTACTGTTGGATTTGCAGAAGCAGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0044238 GO:0070001 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Asp SapB_1 SapB_2

Protein Analysis

513

Amino Acids

55.1

Weight (kDa)

4.99

Isoelectric Point (pI)

30.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	86 - 512	6.6e-116	Eukaryotic aspartyl protease
TAXi_N	PF14543	87 - 245	5.9e-11	Xylanase inhibitor N-terminal
SapB_2	PF03489	320 - 353	5.1e-12	Saposin-like type B, region 2
SapB_1	PF05184	384 - 422	9e-10	Saposin-like type B, region 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001862)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G11910 AT1G11910 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290 AT1G62290
fragaria_vesca	FvH4_3g23140 FvH4_3g23140 FvH4_6g42880 FvH4_6g42880
malus_domestica	MD00G1159300.v1.1 MD09G1111100.v1.1 MD11G1213600.v1.1 MD15G1413400.v1.1 MD17G1098900.v1.1 MD17G1282600.v1.1 MD17G1282700.v1.1
prunus_persica	Prupe.3G214900_v2.0.a1 Prupe.3G214900_v2.0.a1 Prupe.4G217700_v2.0.a1
pyrus_communis	pycom03g14920 pycom09g03360 pycom11g18820 pycom17g09430
rosa_chinensis	RchiOBHm_Chr2g0158811 RchiOBHm_Chr5g0040781
rosa_laevigata	RLG00000021115 RLG00000034001
rosa_multiflora	Rmu_sc0001579.1_g000001
rosa_roxburghii	Rroxscaffold_1G00039910 Rroxscaffold_2G00090980
rosa_rugosa	Rorug02G0472700 Rorug05G0187200 Rorug05G0187300
rosa_samantha	Rh2AG538500 Rh2BG551200 Rh2CG521800 Rh2DG561000 Rh5AG273000 Rh5BG278100 Rh5CG310100 Rh5DG286000
rosa_wichuraiana	Rw2G044570 Rw5G025670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	295
AasI	GACNNNNNNGTC	1 cut(s)	1266
AbsI	CCTCGAGG	1 cut(s)	1443
Acc36I	ACCTGC	2 cut(s)	295, 455
Acc65I	GGTACC	1 cut(s)	5
AccB1I	GGYRCC	2 cut(s)	5, 337
AccB7I	CCANNNNNTGG	1 cut(s)	754
AccI	GTMKAC	1 cut(s)	580
AccII	CGCG	1 cut(s)	198
AciI	CCGC	3 cut(s)	196, 198, 434
AclWI	GGATC	4 cut(s)	120, 167, 522, 557
AcuI	CTGAAG	2 cut(s)	257, 720
AdeI	CACNNNGTG	1 cut(s)	1495
AfaI	GTAC	5 cut(s)	7, 446, 1060, 1156, 1370
AfiI	CCNNNNNNNGG	6 cut(s)	15, 74, 75, 202, 754, 1445
AgsI	TTSAA	6 cut(s)	181, 233, 599, 1097, 1234, 1292
AjnI	CCWGG	2 cut(s)	477, 544
AleI	CACNNNNGTG	1 cut(s)	557
AloI	GAACNNNNNNTCC	2 cut(s)	606, 638
Alw21I	GWGCWC	2 cut(s)	60, 1251
Alw26I	GTCTC	2 cut(s)	131, 197
AlwI	GGATC	4 cut(s)	120, 167, 522, 557
AlwNI	CAGNNNCTG	1 cut(s)	37
Ama87I	CYCGRG	1 cut(s)	1443
AoxI	GGCC	1 cut(s)	899
ApeKI	GCWGC	3 cut(s)	563, 1148, 1535
Asp718I	GGTACC	1 cut(s)	5
AspLEI	GCGC	1 cut(s)	458
AspS9I	GGNCC	3 cut(s)	481, 899, 1448
AsuHPI	GGTGA	7 cut(s)	31, 115, 728, 770, 842, 1289, 1397
AvaI	CYCGRG	1 cut(s)	1443
AvaII	GGWCC	2 cut(s)	481, 1448
BaeGI	GKGCMC	1 cut(s)	342
BanI	GGYRCC	2 cut(s)	5, 337
BanII	GRGCYC	1 cut(s)	1251
BauI	CACGAG	1 cut(s)	1074
Bbv12I	GWGCWC	2 cut(s)	60, 1251
BbvI	GCAGC	2 cut(s)	575, 1135
BccI	CCATC	6 cut(s)	68, 569, 730, 1127, 1162, 1340
BceAI	ACGGC	1 cut(s)	1413
BciT130I	CCWGG	2 cut(s)	479, 546
BciVI	GTATCC	1 cut(s)	1176
BcoDI	GTCTC	2 cut(s)	131, 197
BfaI	CTAG	3 cut(s)	396, 852, 1427
BfuAI	ACCTGC	2 cut(s)	295, 455
BfuI	GTATCC	1 cut(s)	1176
BglII	AGATCT	2 cut(s)	603, 1227
BisI	GCNGC	4 cut(s)	435, 564, 1149, 1536
BlsI	GCNGC	4 cut(s)	436, 565, 1150, 1537
Bme1390I	CCNGG	2 cut(s)	479, 546
Bme18I	GGWCC	2 cut(s)	481, 1448
BmeT110I	CYCGRG	1 cut(s)	1443
BmgT120I	GGNCC	3 cut(s)	481, 899, 1448
BmiI	GGNNCC	4 cut(s)	7, 339, 543, 1437
BmrFI	CCNGG	2 cut(s)	479, 546
BmrI	ACTGGG	2 cut(s)	1260, 1311
BmsI	GCATC	3 cut(s)	241, 950, 1135
BmuI	ACTGGG	2 cut(s)	1260, 1311
BpmI	CTGGAG	1 cut(s)	1344
Bpu10I	CCTNAGC	1 cut(s)	1017
BsaAI	YACGTR	1 cut(s)	754
BsaJI	CCNNGG	4 cut(s)	196, 342, 537, 545
BsaXI	ACNNNNNCTCC	4 cut(s)	606, 636, 1289, 1319
Bsc4I	CCNNNNNNNGG	6 cut(s)	15, 74, 75, 202, 754, 1445
Bse1I	ACTGG	9 cut(s)	322, 442, 457, 668, 776, 982, 1005, 1266, 1317
BseBI	CCWGG	2 cut(s)	479, 546
BseDI	CCNNGG	4 cut(s)	196, 342, 537, 545
BseGI	GGATG	4 cut(s)	256, 741, 820, 1114
BseLI	CCNNNNNNNGG	6 cut(s)	15, 74, 75, 202, 754, 1445
BseMII	CTCAG	5 cut(s)	137, 269, 305, 621, 1031
BseNI	ACTGG	9 cut(s)	322, 442, 457, 668, 776, 982, 1005, 1266, 1317
BseRI	GAGGAG	2 cut(s)	1235, 1297
BseSI	GKGCMC	1 cut(s)	342
BseXI	GCAGC	2 cut(s)	575, 1135
Bsh1236I	CGCG	1 cut(s)	198
BshFI	GGCC	1 cut(s)	901
BshNI	GGYRCC	2 cut(s)	5, 337
BsiHKAI	GWGCWC	2 cut(s)	60, 1251
BsiHKCI	CYCGRG	1 cut(s)	1443
BslFI	GGGAC	4 cut(s)	31, 230, 439, 1084
BslI	CCNNNNNNNGG	6 cut(s)	15, 74, 75, 202, 754, 1445
BsmAI	GTCTC	2 cut(s)	131, 197
BsmBI	CGTCTC	1 cut(s)	197
BsmFI	GGGAC	4 cut(s)	31, 230, 439, 1084
BsmI	GAATGC	1 cut(s)	157
BsnI	GGCC	1 cut(s)	901
BsoBI	CYCGRG	1 cut(s)	1443
Bsp1286I	GDGCHC	3 cut(s)	60, 342, 1251
Bsp143I	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
BspACI	CCGC	3 cut(s)	196, 198, 434
BspANI	GGCC	1 cut(s)	901
BspCNI	CTCAG	5 cut(s)	136, 268, 304, 620, 1030
BspFNI	CGCG	1 cut(s)	198
BspLI	GGNNCC	4 cut(s)	7, 339, 543, 1437
BspMI	ACCTGC	2 cut(s)	295, 455
BspPI	GGATC	4 cut(s)	120, 167, 522, 557
BspT107I	GGYRCC	2 cut(s)	5, 337
BsrI	ACTGG	9 cut(s)	322, 442, 457, 668, 776, 982, 1005, 1266, 1317
BssECI	CCNNGG	4 cut(s)	196, 342, 537, 545
BssMI	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
BssNAI	GTATAC	1 cut(s)	581
BssSI	CACGAG	1 cut(s)	1074
BssT1I	CCWWGG	1 cut(s)	537
Bst1107I	GTATAC	1 cut(s)	581
Bst2BI	CACGAG	1 cut(s)	1074
Bst2UI	CCWGG	2 cut(s)	479, 546
Bst4CI	ACNGT	6 cut(s)	304, 493, 1102, 1455, 1495, 1519
BstAPI	GCANNNNNTGC	1 cut(s)	1157
BstBAI	YACGTR	1 cut(s)	754
BstC8I	GCNNGC	2 cut(s)	370, 1014
BstDEI	CTNAG	9 cut(s)	123, 147, 255, 291, 567, 607, 1017, 1409, 1539
BstDSI	CCRYGG	1 cut(s)	196
BstF5I	GGATG	4 cut(s)	256, 741, 820, 1114
BstFNI	CGCG	1 cut(s)	198
BstHHI	GCGC	1 cut(s)	458
BstKTI	GATC	6 cut(s)	115, 175, 517, 552, 606, 1230
BstMAI	GTCTC	2 cut(s)	131, 197
BstMBI	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
BstMWI	GCNNNNNNNGC	4 cut(s)	152, 938, 1157, 1535
BstNI	CCWGG	2 cut(s)	479, 546
BstNSI	RCATGY	1 cut(s)	1155
BstSCI	CCNGG	2 cut(s)	477, 544
BstSLI	GKGCMC	1 cut(s)	342
BstUI	CGCG	1 cut(s)	198
BstV1I	GCAGC	2 cut(s)	575, 1135
BstX2I	RGATCY	3 cut(s)	172, 603, 1227
BstYI	RGATCY	3 cut(s)	172, 603, 1227
BstZ17I	GTATAC	1 cut(s)	581
BsuI	GTATCC	1 cut(s)	1176
BsuRI	GGCC	1 cut(s)	901
BtgI	CCRYGG	1 cut(s)	196
BtsCI	GGATG	4 cut(s)	256, 741, 820, 1114
BtsIMutI	CAGTG	5 cut(s)	219, 696, 783, 1451, 1500
BveI	ACCTGC	2 cut(s)	295, 455
Cac8I	GCNNGC	2 cut(s)	370, 1014
CaiI	CAGNNNCTG	1 cut(s)	37
CfoI	GCGC	1 cut(s)	458
Cfr13I	GGNCC	3 cut(s)	481, 899, 1448
Cfr42I	CCGCGG	1 cut(s)	199
Csp6I	GTAC	5 cut(s)	6, 445, 1059, 1155, 1369
CviAII	CATG	6 cut(s)	50, 485, 929, 1142, 1152, 1477
CviQI	GTAC	5 cut(s)	6, 445, 1059, 1155, 1369
DdeI	CTNAG	9 cut(s)	123, 147, 255, 291, 567, 607, 1017, 1409, 1539
DpnI	GATC	6 cut(s)	114, 174, 516, 551, 605, 1229
DpnII	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
DraIII	CACNNNGTG	1 cut(s)	1495
DrdI	GACNNNNNNGTC	1 cut(s)	1266
DseDI	GACNNNNNNGTC	1 cut(s)	1266
Ecl136II	GAGCTC	1 cut(s)	1249
Eco130I	CCWWGG	1 cut(s)	537
Eco24I	GRGCYC	1 cut(s)	1251
Eco47I	GGWCC	2 cut(s)	481, 1448
Eco53kI	GAGCTC	1 cut(s)	1249
Eco57I	CTGAAG	2 cut(s)	257, 720
Eco88I	CYCGRG	1 cut(s)	1443
EcoICRI	GAGCTC	1 cut(s)	1249
EcoRII	CCWGG	2 cut(s)	477, 544
EcoT14I	CCWWGG	1 cut(s)	537
EcoT38I	GRGCYC	1 cut(s)	1251
ErhI	CCWWGG	1 cut(s)	537
Esp3I	CGTCTC	1 cut(s)	197
FaeI	CATG	6 cut(s)	53, 488, 932, 1145, 1155, 1480
FalI	AAGNNNNNCTT	2 cut(s)	341, 373
FaqI	GGGAC	4 cut(s)	31, 230, 439, 1084
FatI	CATG	6 cut(s)	49, 484, 928, 1141, 1151, 1476
FauNDI	CATATG	1 cut(s)	769
FblI	GTMKAC	1 cut(s)	580
Fnu4HI	GCNGC	4 cut(s)	435, 564, 1149, 1536
FokI	GGATG	4 cut(s)	263, 748, 827, 1121
FriOI	GRGCYC	1 cut(s)	1251
Fsp4HI	GCNGC	4 cut(s)	435, 564, 1149, 1536
FspBI	CTAG	3 cut(s)	396, 852, 1427
GlaI	GCGC	1 cut(s)	457
GluI	GCNGC	4 cut(s)	435, 564, 1149, 1536
GsuI	CTGGAG	1 cut(s)	1344
HaeIII	GGCC	1 cut(s)	901
HhaI	GCGC	1 cut(s)	458
Hin1II	CATG	6 cut(s)	53, 488, 932, 1145, 1155, 1480
Hin6I	GCGC	1 cut(s)	456
HinP1I	GCGC	1 cut(s)	456
HincII	GTYRAC	1 cut(s)	979
HindII	GTYRAC	1 cut(s)	979
HphI	GGTGA	7 cut(s)	31, 115, 728, 770, 842, 1289, 1397
Hpy166II	GTNNAC	5 cut(s)	300, 410, 581, 979, 1256
Hpy188I	TCNGA	4 cut(s)	117, 294, 1132, 1215
Hpy188III	TCNNGA	7 cut(s)	518, 599, 840, 879, 1231, 1361, 1427
Hpy8I	GTNNAC	5 cut(s)	300, 410, 581, 979, 1256
HpyAV	CCTTC	3 cut(s)	175, 695, 704
HpyCH4III	ACNGT	6 cut(s)	304, 493, 1102, 1455, 1495, 1519
HpyCH4IV	ACGT	2 cut(s)	753, 1371
HpyCH4V	TGCA	7 cut(s)	155, 464, 932, 1141, 1151, 1241, 1529
HpyF10VI	GCNNNNNNNGC	4 cut(s)	152, 938, 1157, 1535
HpyF3I	CTNAG	9 cut(s)	123, 147, 255, 291, 567, 607, 1017, 1409, 1539
HpySE526I	ACGT	2 cut(s)	753, 1371
Hsp92II	CATG	6 cut(s)	53, 488, 932, 1145, 1155, 1480
HspAI	GCGC	1 cut(s)	456
KpnI	GGTACC	1 cut(s)	9
KspI	CCGCGG	1 cut(s)	199
Kzo9I	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
LmnI	GCTCC	6 cut(s)	541, 938, 1222, 1363, 1391, 1441
Lsp1109I	GCAGC	2 cut(s)	575, 1135
LweI	GCATC	3 cut(s)	241, 950, 1135
MaeI	CTAG	3 cut(s)	396, 852, 1427
MaeII	ACGT	2 cut(s)	753, 1371
MaeIII	GTNAC	5 cut(s)	19, 353, 487, 778, 1295
MalI	GATC	6 cut(s)	114, 174, 516, 551, 605, 1229
MboI	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
MboII	GAAGA	3 cut(s)	201, 250, 719
MfeI	CAATTG	3 cut(s)	459, 867, 933
MflI	RGATCY	3 cut(s)	172, 603, 1227
MhlI	GDGCHC	3 cut(s)	60, 342, 1251
MlyI	GAGTC	3 cut(s)	16, 775, 1066
MmeI	TCCRAC	2 cut(s)	592, 1501
MnlI	CCTC	8 cut(s)	352, 523, 608, 1213, 1318, 1381, 1439, 1452
MseI	TTAA	5 cut(s)	96, 660, 686, 1023, 1209
MslI	CAYNNNNRTG	2 cut(s)	557, 768
MspA1I	CMGCKG	2 cut(s)	198, 1286
MspR9I	CCNGG	2 cut(s)	479, 546
MunI	CAATTG	3 cut(s)	459, 867, 933
Mva1269I	GAATGC	1 cut(s)	157
MvaI	CCWGG	2 cut(s)	479, 546
MvnI	CGCG	1 cut(s)	198
MwoI	GCNNNNNNNGC	4 cut(s)	152, 938, 1157, 1535
NdeI	CATATG	1 cut(s)	769
NdeII	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
NlaIII	CATG	6 cut(s)	53, 488, 932, 1145, 1155, 1480
NlaIV	GGNNCC	4 cut(s)	7, 339, 543, 1437
NmeAIII	GCCGAG	1 cut(s)	323
NmuCI	GTSAC	3 cut(s)	19, 487, 778
NspI	RCATGY	1 cut(s)	1155
OliI	CACNNNNGTG	1 cut(s)	557
PaeR7I	CTCGAG	1 cut(s)	1443
PaqCI	CACCTGC	1 cut(s)	295
PctI	GAATGC	1 cut(s)	157
PfeI	GAWTC	8 cut(s)	206, 499, 740, 875, 1035, 1189, 1280, 1459
PflFI	GACNNNGTC	1 cut(s)	485
PflMI	CCANNNNNTGG	1 cut(s)	754
PkrI	GCNGC	4 cut(s)	436, 565, 1150, 1537
PleI	GAGTC	3 cut(s)	16, 775, 1066
PpsI	GAGTC	3 cut(s)	16, 775, 1066
Ppu21I	YACGTR	1 cut(s)	754
Psp124BI	GAGCTC	1 cut(s)	1251
Psp6I	CCWGG	2 cut(s)	477, 544
PspGI	CCWGG	2 cut(s)	477, 544
PspN4I	GGNNCC	4 cut(s)	7, 339, 543, 1437
PspPI	GGNCC	3 cut(s)	481, 899, 1448
PspXI	VCTCGAGB	1 cut(s)	1443
PstNI	CAGNNNCTG	1 cut(s)	37
PsuI	RGATCY	3 cut(s)	172, 603, 1227
PsyI	GACNNNGTC	1 cut(s)	485
PvuII	CAGCTG	1 cut(s)	1286
RsaI	GTAC	5 cut(s)	7, 446, 1060, 1156, 1370
RsaNI	GTAC	5 cut(s)	6, 445, 1059, 1155, 1369
RseI	CAYNNNNRTG	2 cut(s)	557, 768
SacI	GAGCTC	1 cut(s)	1251
SacII	CCGCGG	1 cut(s)	199
SaqAI	TTAA	5 cut(s)	96, 660, 686, 1023, 1209
SatI	GCNGC	4 cut(s)	435, 564, 1149, 1536
Sau3AI	GATC	6 cut(s)	112, 172, 514, 549, 603, 1227
Sau96I	GGNCC	3 cut(s)	481, 899, 1448
SchI	GAGTC	3 cut(s)	16, 775, 1066
ScrFI	CCNGG	2 cut(s)	479, 546
SduI	GDGCHC	3 cut(s)	60, 342, 1251
SfaNI	GCATC	3 cut(s)	241, 950, 1135
Sfr274I	CTCGAG	1 cut(s)	1443
Sfr303I	CCGCGG	1 cut(s)	199
SgrBI	CCGCGG	1 cut(s)	199
SinI	GGWCC	2 cut(s)	481, 1448
SlaI	CTCGAG	1 cut(s)	1443
SmiMI	CAYNNNNRTG	2 cut(s)	557, 768
SmlI	CTYRAG	1 cut(s)	1443
SmoI	CTYRAG	1 cut(s)	1443
SsiI	CCGC	3 cut(s)	196, 198, 434
SspMI	CTAG	3 cut(s)	396, 852, 1427
SstI	GAGCTC	1 cut(s)	1251
StyD4I	CCNGG	2 cut(s)	477, 544
StyI	CCWWGG	1 cut(s)	537
TaaI	ACNGT	6 cut(s)	304, 493, 1102, 1455, 1495, 1519
TaiI	ACGT	2 cut(s)	756, 1374
TaqI	TCGA	2 cut(s)	1444, 1500
TatI	WGTACW	2 cut(s)	1058, 1154
TauI	GCSGC	1 cut(s)	437
TfiI	GAWTC	8 cut(s)	206, 499, 740, 875, 1035, 1189, 1280, 1459
Tru1I	TTAA	5 cut(s)	96, 660, 686, 1023, 1209
Tru9I	TTAA	5 cut(s)	96, 660, 686, 1023, 1209
TscAI	CASTG	5 cut(s)	226, 703, 783, 1458, 1500
TseFI	GTSAC	3 cut(s)	19, 487, 778
TseI	GCWGC	3 cut(s)	563, 1148, 1535
Tsp45I	GTSAC	3 cut(s)	19, 487, 778
TspDTI	ATGAA	2 cut(s)	666, 1465
TspGWI	ACGGA	1 cut(s)	462
TspRI	CASTG	5 cut(s)	226, 703, 783, 1458, 1500
Tth111I	GACNNNGTC	1 cut(s)	485
Van91I	CCANNNNNTGG	1 cut(s)	754
VpaK11BI	GGWCC	2 cut(s)	481, 1448
XbaI	TCTAGA	1 cut(s)	1426
XceI	RCATGY	1 cut(s)	1155
XcmI	CCANNNNNNNNNTGG	3 cut(s)	76, 449, 491
XhoI	CTCGAG	1 cut(s)	1443
XmiI	GTMKAC	1 cut(s)	580
XspI	CTAG	3 cut(s)	396, 852, 1427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.